LOCUS       Exported                7611 bp ds-DNA     circular UNA 21-NOV-2019
DEFINITION  natural circular DNA
ACCESSION   urn.local...3t-b851spn
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Nov 22, 2019 from SnapGene Viewer 5.0.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7611
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     promoter        275..356
                     /standard_name="CMV prom"
                     /label=CMV prom promoter
                     /note="Geneious type: Promoter"
     promoter        377..395
                     /standard_name="T7 prom"
                     /label=T7 prom promoter
                     /note="Geneious type: Promoter"
     RBS             403..408
                     /standard_name="mRBS"
                     /label=mRBS
     CDS             409..4617
                     /standard_name="SpCas9"
                     /label=SpCas9
     sig_peptide     412..465
                     /standard_name="BP_NLS"
                     /label=BP_NLS
     CDS             466..4566
                     /standard_name="SpCas9"
                     /label=SpCas9
                     /note="hSpCas9"
                     /note="Geneious type: gene"
     misc_feature    1426..1428
                     /standard_name="I322V"
                     /label=I322V
                     /note="Geneious type: Mutation"
     misc_feature    1687..1689
                     /standard_name="S409I"
                     /label=S409I
                     /note="Geneious type: Mutation"
     misc_feature    1741..1743
                     /standard_name="E427G"
                     /label=E427G
                     /note="Geneious type: Mutation"
     misc_feature    2422..2424
                     /standard_name="R654L"
                     /label=R654L
                     /note="Geneious type: Mutation"
     misc_feature    2719..2721
                     /standard_name="R753G"
                     /label=R753G
                     /note="Geneious type: Mutation"
     misc_feature    3802..3804
                     /standard_name="R1114G"
                     /label=R1114G
                     /note="Geneious type: Mutation"
     misc_feature    3865..3867
                     /standard_name="D1135N"
                     /label=D1135N
                     /note="Geneious type: Mutation"
     misc_feature    4117..4119
                     /standard_name="E1219V"
                     /label=E1219V
                     /note="Geneious type: Mutation"
     misc_feature    4456..4458
                     /standard_name="D1332N"
                     /label=D1332N
                     /note="Geneious type: Mutation"
     misc_feature    4465..4467
                     /standard_name="R1335Q"
                     /label=R1335Q
                     /note="Geneious type: Mutation"
     misc_feature    4471..4473
                     /standard_name="T1337N"
                     /label=T1337N
                     /note="Geneious type: Mutation"
     misc_feature    4474..4476
                     /standard_name="S1339T"
                     /label=S1339T
                     /note="Geneious type: Mutation"
     misc_feature    4507..4509
                     /standard_name="H1349R"
                     /label=H1349R
                     /note="Geneious type: Mutation"
     sig_peptide     4567..4617
                     /standard_name="BP_NLS"
                     /label=BP_NLS
     terminator      4669..4896
                     /standard_name="bGH_PA term"
                     /label=bGH_PA term terminator
                     /note="Geneious type: Terminator"
     promoter        complement(5016..5045)
                     /standard_name="lac prom"
                     /label=lac prom promoter
                     /note="Geneious type: Promoter"
     misc_feature    complement(5354..5973)
                     /standard_name="pBR322 origin"
                     /label=pBR322 origin
                     /note="Geneious type: Origin of Replication"
     misc_feature    complement(6128..6988)
                     /standard_name="amp marker"
                     /label=amp marker
                     /note="Geneious type: Marker"
     promoter        complement(7030..7058)
                     /standard_name="amp prom"
                     /label=amp prom promoter
                     /note="Geneious type: Promoter"
     promoter        join(7379..7611,1..356)
                     /standard_name="CMV_Full"
                     /label=CMV_Full promoter
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca
      421 gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcgacaa gaagtacagc
      481 atcggcctgg acatcggcac caactctgtg ggctgggccg tgatcaccga cgagtacaag
      541 gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat caagaagaac
      601 ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg gctgaagaga
      661 accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca agagatcttc
      721 agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga gtccttcctg
      781 gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg
      841 gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt ggacagcacc
      901 gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa gttccggggc
      961 cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa gctgttcatc
     1021 cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg
     1081 gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga aaatctgatc
     1141 gcccagctgc ccggcgagaa gaagaatggc ctgttcggaa acctgattgc cctgagcctg
     1201 ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa actgcagctg
     1261 agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg cgaccagtac
     1321 gccgacctgt ttctggccgc caagaacctg tccgacgcca tcctgctgag cgacatcctg
     1381 agagtgaaca ccgagatcac caaggccccc ctgagcgcct ctatggtgaa gagatacgac
     1441 gagcaccacc aggacctgac cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag
     1501 tacaaagaga ttttcttcga ccagagcaag aacggctacg ccggctacat tgacggcgga
     1561 gccagccagg aagagttcta caagttcatc aagcccatcc tggaaaagat ggacggcacc
     1621 gaggaactgc tcgtgaagct gaacagagag gacctgctgc ggaagcagcg gaccttcgac
     1681 aacggcatta tcccccacca gatccacctg ggagagctgc acgccattct gcggcggcag
     1741 ggcgattttt acccattcct gaaggacaac cgggaaaaga tcgagaagat cctgaccttc
     1801 cgcatcccct actacgtggg ccctctggcc aggggaaaca gcagattcgc ctggatgacc
     1861 agaaagagcg aggaaaccat caccccctgg aacttcgagg aagtggtgga caagggcgct
     1921 tccgcccaga gcttcatcga gcggatgacc aacttcgata agaacctgcc caacgagaag
     1981 gtgctgccca agcacagcct gctgtacgag tacttcaccg tgtataacga gctgaccaaa
     2041 gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc tgagcggcga gcagaaaaag
     2101 gccatcgtgg acctgctgtt caagaccaac cggaaagtga ccgtgaagca gctgaaagag
     2161 gactacttca agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg
     2221 ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa ttatcaagga caaggacttc
     2281 ctggacaatg aggaaaacga ggacattctg gaagatatcg tgctgaccct gacactgttt
     2341 gaggacagag agatgatcga ggaacggctg aaaacctatg cccacctgtt cgacgacaaa
     2401 gtgatgaagc agctgaagcg gctgagatac accggctggg gcaggctgag ccggaagctg
     2461 atcaacggca tccgggacaa gcagtccggc aagacaatcc tggatttcct gaagtccgac
     2521 ggcttcgcca acagaaactt catgcagctg atccacgacg acagcctgac ctttaaagag
     2581 gacatccaga aagcccaggt gtccggccag ggcgatagcc tgcacgagca cattgccaat
     2641 ctggccggca gccccgccat taagaagggc atcctgcaga cagtgaaggt ggtggacgag
     2701 ctcgtgaaag tgatgggcgg ccacaagccc gagaacatcg tgatcgaaat ggccagagag
     2761 aaccagacca cccagaaggg acagaagaac agccgcgaga gaatgaagcg gatcgaagag
     2821 ggcatcaaag agctgggcag ccagatcctg aaagaacacc ccgtggaaaa cacccagctg
     2881 cagaacgaga agctgtacct gtactacctg cagaatgggc gggatatgta cgtggaccag
     2941 gaactggaca tcaaccggct gtccgactac gatgtggacc atatcgtgcc tcagagcttt
     3001 ctgaaggacg actccatcga caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag
     3061 agcgacaacg tgccctccga agaggtcgtg aagaagatga agaactactg gcggcagctg
     3121 ctgaacgcca agctgattac ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc
     3181 ggcctgagcg aactggataa ggccggcttc atcaagagac agctggtgga aacccggcag
     3241 atcacaaagc acgtggcaca gatcctggac tcccggatga acactaagta cgacgagaat
     3301 gacaagctga tccgggaagt gaaagtgatc accctgaagt ccaagctggt gtccgatttc
     3361 cggaaggatt tccagtttta caaagtgcgc gagatcaaca actaccacca cgcccacgac
     3421 gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc
     3481 gagttcgtgt acggcgacta caaggtgtac gacgtgcgga agatgatcgc caagagcgag
     3541 caggaaatcg gcaaggctac cgccaagtac ttcttctaca gcaacatcat gaactttttc
     3601 aagaccgaga ttaccctggc caacggcgag atccggaagc ggcctctgat cgagacaaac
     3661 ggcgaaaccg gggagatcgt gtgggataag ggccgggatt ttgccaccgt gcggaaagtg
     3721 ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc
     3781 aaagagtcta tcctgcccaa gggcaacagc gataagctga tcgccagaaa gaaggactgg
     3841 gaccctaaga agtacggcgg cttcaacagc cccaccgtgg cctattctgt gctggtggtg
     3901 gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct gctggggatc
     3961 accatcatgg aaagaagcag cttcgagaag aatcccatcg actttctgga agccaagggc
     4021 tacaaagaag tgaaaaagga cctgatcatc aagctgccta agtactccct gttcgagctg
     4081 gaaaacggcc ggaagagaat gctggcctct gccggcgtgc tgcagaaggg aaacgaactg
     4141 gccctgccct ccaaatatgt gaacttcctg tacctggcca gccactatga gaagctgaag
     4201 ggctcccccg aggataatga gcagaaacag ctgtttgtgg aacagcacaa gcactacctg
     4261 gacgagatca tcgagcagat cagcgagttc tccaagagag tgatcctggc cgacgctaat
     4321 ctggacaaag tgctgtccgc ctacaacaag caccgggata agcccatcag agagcaggcc
     4381 gagaatatca tccacctgtt taccctgacc aatctgggag cccctgccgc cttcaagtac
     4441 tttgacacca ccatcaaccg gaagcaatac aacacgacca aagaggtgct ggacgccacc
     4501 ctgatccgtc agagcatcac cggcctgtac gagacacgga tcgacctgtc tcagctggga
     4561 ggtgacaaaa gaaccgccga cggcagcgaa ttcgagccca agaagaagag gaaagtctaa
     4621 ccggtcatca tcaccatcac cattgagttt aaacccgctg atcagcctcg actgtgcctt
     4681 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     4741 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     4801 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     4861 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     4921 ggggctcgat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     4981 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     5041 gtaaagccta gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     5101 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     5161 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     5221 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     5281 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     5341 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     5401 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     5461 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     5521 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     5581 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     5641 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     5701 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     5761 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     5821 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     5881 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     5941 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     6001 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     6061 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     6121 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     6181 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     6241 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     6301 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     6361 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     6421 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     6481 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     6541 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     6601 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     6661 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     6721 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     6781 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     6841 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     6901 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     6961 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     7021 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     7081 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     7141 gatctcccga tcccctaggg tcgactctca gtacaatctg ctctgatgcc gcatagttaa
     7201 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt
     7261 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc
     7321 gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta
     7381 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     7441 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     7501 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     7561 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat c
//
