LOCUS       Exported                5409 bp ds-DNA     circular SYN 11-FEB-2020
DEFINITION  [pSC101] pro1 rpoZ-cas9 1-573 npuN bla.
ACCESSION   urn.local...g-b84xafm
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 11, 2020 from SnapGene 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5409
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(1..951)
                     /label=RepA
                     /note="RepA"
                     /note="pSC101 origin of replication protein"
     rep_origin      complement(955..1700)
                     /direction=LEFT
     terminator      1716..1760
                     /note="rrnB1 transcriptional terminator"
     promoter        1839..1879
                     /standard_name="pro5"
                     /label=pro5 promoter
                     /note="pro1 (Insulated Promoter - based on rrnB1 P1)"
     -35_signal      1845..1850
                     /standard_name="-35 "
                     /label=-35
                     /note="-35"
     -10_signal      1868..1873
                     /standard_name="-10"
                     /label=-10
     RBS             1942..1955
                     /standard_name="sd8"
                     /label=sd8
     CDS             1956..4259
     gene            1959..2228
                     /standard_name="rpoZ"
                     /label=rpoZ
     gene            2235..3950
                     /standard_name="SpCas9-m4 to 573"
                     /label=SpCas9-m4 to 573
     misc_feature    2260..2261
                     /standard_name="D10T"
                     /label=D10T
                     /note="Geneious type: Mutation"
     misc_feature    3196..3197
                     /standard_name="I322V"
                     /label=I322V
                     /note="Geneious type: Mutation"
     misc_feature    3456..3458
                     /standard_name="S409I"
                     /label=S409I
                     /note="Geneious type: Mutation"
     misc_feature    3510
                     /standard_name="E427G"
                     /label=E427G
                     /note="Geneious type: Mutation"
     gene            3951..4256
                     /standard_name="npuN"
                     /label=npuN
     terminator      complement(4283..4324)
                     /note="tetA/orfL bidirectional terminator"
                     /note="termination of bla transcript is slightly weaker
                     than opposite direction"
     CDS             complement(4338..5198)
                     /label=bla (AmpR)
                     /note="bla (AmpR)"
                     /note="from pUC19"
     promoter        complement(5199..5393)
                     /note="bla promoter"
                     /note="from pUC19"
     modified_base   5394..5409
                     /label=USER junction
                     /note="USER junction"
ORIGIN
        1 tcagatcctt ccgtatttag ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag
       61 ccatgagaac gaaccattga gatcatgctt actttgcatg tcactcaaaa attttgcctc
      121 aaaactggtg agctgaattt ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt
      181 acgtaggtag gaatctgatg taatggttgt tggtattttg tcaccattca tttttatctg
      241 gttgttctca agttcggtta cgagatccat ttgtctatct agttcaactt ggaaaatcaa
      301 cgtatcagtc gggcggcctc gcttatcaac caccaatttc atattgctgt aagtgtttaa
      361 atctttactt attggtttca aaacccattg gttaagcctt ttaaactcat ggtagttatt
      421 ttcaagcatt aacatgaact taaattcatc aaggctaatc tctatatttg ccttgtgagt
      481 tttcttttgt gttagttctt ttaataacca ctcataaatc ctcatagagt atttgttttc
      541 aaaagactta acatgttcca gattatattt tatgaatttt tttaactgga aaagataagg
      601 caatatctct tcactaaaaa ctaattctaa tttttcgctt gagaacttgg catagtttgt
      661 ccactggaaa atctcaaagc ctttaaccaa aggattcctg atttccacag ttctcgtcat
      721 cagctctctg gttgctttag ctaatacacc ataagcattt tccctactga tgttcatcat
      781 ctgagcgtat tggttataag tgaacgatac cgtccgttct ttccttgtag ggttttcaat
      841 cgtggggttg agtagtgcca cacagcataa aattagcttg gtttcatgct ccgttaagtc
      901 atagcgacta atcgctagtt catttgcttt gaaaacaact aattcagaca tacatctcaa
      961 ttggtctagg tgattttaat cactatacca attgagatgg gctagtcaat gataattact
     1021 agtccttttc ctttgagttg tgggtatctg taaattctgc tagacctttg ctggaaaact
     1081 tgtaaattct gctagaccct ctgtaaattc cgctagacct ttgtgtgttt tttttgttta
     1141 tattcaagtg gttataattt atagaataaa gaaagaataa aaaaagataa aaagaataga
     1201 tcccagccct gtgtataact cactacttta gtcagttccg cagtattaca aaaggatgtc
     1261 gcaaacgctg tttgctcctc tacaaaacag accttaaaac cctaaaggct taagtagcac
     1321 cctcgcaagc tcgggcaaat cgctgaatat tccttttgtc tccgaccatc aggcacctga
     1381 gtcgctgtct ttttcgtgac attcagttcg ctgcgctcac ggctctggca gtgaatgggg
     1441 gtaaatggca ctacaggcgc cttttatgga ttcatgcaag gaaactaccc ataatacaag
     1501 aaaagcccgt cacgggcttc tcagggcgtt ttatggcggg tctgctatgt ggtgctatct
     1561 gactttttgc tgttcagcag ttcctgccct ctgattttcc agtctgacca cttcggatta
     1621 tcccgtgaca ggtcattcag actggctaat gcacccagta aggcagcggt atcatcaaca
     1681 ggcttacccg tcttactgtc aagaggacat ccggtcaaat aaaacgaaag gctcagtcga
     1741 aagactgggc ctttcgtttt gctgaggaga cttagggacc ctcacagcta acaccacgtc
     1801 gtccctatct gctgccctag gtctatgagt ggttgctgga taactttacg ggcatgcata
     1861 aggctcgtag gatatattca gggagaccac aacggtttcc ctctacaaat aattttgttt
     1921 aactttacct gcaggtgcag taaaggaaaa aaaaaatggc acgcgtaact gttcaggacg
     1981 ctgtagagaa aaatggtaac cgttttgacc tggtactggt cgccgcgcgt cgcgctcgtc
     2041 agatgcaggt aggcggaaag gatccgctgg taccggaaga aaacgataaa accactgtaa
     2101 tcgcgctgcg cgaaatcgaa gaaggtctga tcaacaacca gatcctcgac gttcgcgaac
     2161 gccaggaaca gcaagagcag gaagccgctg aattacaagc cgttaccgct attgctgaag
     2221 gtcgtcgtgc tgcagacaag aagtactcca ttgggctcgc tatcggcaca aacagcgtcg
     2281 gctgggccgt cattacggac gagtacaagg tgccgagcaa aaaattcaaa gttctgggca
     2341 ataccgatcg ccacagcata aagaagaacc tcattggcgc cctcctgttc gactccgggg
     2401 agacggccga agccacgcgg ctcaaaagaa cagcacggcg cagatatacc cgcagaaaga
     2461 atcggatctg ctacctgcag gagatcttta gtaatgagat ggctaaggtg gatgactctt
     2521 tcttccatag gctggaggag tcctttttgg tggaggagga taaaaagcac gagcgccacc
     2581 caatctttgg caatatcgtg gacgaggtgg cgtaccatga aaagtaccca accatatatc
     2641 atctgaggaa gaagcttgta gacagtactg ataaggctga cttgcggttg atctatctcg
     2701 cgctggcgca tatgatcaaa tttcggggac acttcctcat cgagggggac ctgaacccag
     2761 acaacagcga tgtcgacaaa ctctttatcc aactggttca gacttacaat cagcttttcg
     2821 aagagaaccc gatcaacgca tccggagttg acgccaaagc aatcctgagc gctaggctgt
     2881 ccaaatcccg gcggctcgaa aacctcatcg cacagctccc tggggagaag aagaacggcc
     2941 tgtttggtaa tcttatcgcc ctgtcactcg ggctgacccc caactttaaa tctaacttcg
     3001 acctggccga agatgccaag cttcaactga gcaaagacac ctacgatgat gatctcgaca
     3061 atctgctggc ccagatcggc gaccagtacg cagacctttt tttggcggca aagaacctgt
     3121 cagacgccat tctgctgagt gatattctgc gagtgaacac ggagatcacc aaagctccgc
     3181 tgagcgctag tatgatcaag cgctatgatg agcaccacca agacttgact ttgctgaagg
     3241 cccttgtcag acagcaactg cctgagaagt acaaggaaat tttcttcgat cagtctaaaa
     3301 atggctacgc cggatacatt gacggcggag caagccagga ggaattttac aaatttatta
     3361 agcccatctt ggaaaaaatg gacggcaccg aggagctgct ggtaaagctt aacagagaag
     3421 atctgttgcg caaacagcgc actttcgaca atggaagcat cccccaccag attcacctgg
     3481 gcgaactgca cgctatcctc aggcggcaag aggatttcta cccctttttg aaagataaca
     3541 gggaaaagat tgagaaaatc ctcacatttc ggatacccta ctatgtaggc cccctcgccc
     3601 ggggaaattc cagattcgcg tggatgactc gcaaatcaga agagaccatc actccctgga
     3661 acttcgagga agtcgtggat aagggggcct ctgcccagtc cttcatcgaa aggatgacta
     3721 actttgataa aaatctgcct aacgaaaagg tgcttcctaa acactctctg ctgtacgagt
     3781 acttcacagt ttataacgag ctcaccaagg tcaaatacgt cacagaaggg atgagaaagc
     3841 cagcattcct gtctggagag cagaagaaag ctatcgtgga cctcctcttc aagacgaacc
     3901 ggaaagttac cgtgaaacag ctcaaagaag actatttcaa aaagattgaa tgtctcagct
     3961 acgaaaccga aatcttgacc gtcgaatatg gtctgctgcc aatcggcaag attgttgaaa
     4021 aacgtattga atgtacggtc tactcagtgg ataacaacgg caatatctac acccagccgg
     4081 tggcccagtg gcatgaccgt ggtgaacagg aagtgttcga atattgtctg gaagacggat
     4141 ctttaatccg tgccacaaag gatcacaaat ttatgactgt agatggtcag atgctcccaa
     4201 tcgacgaaat ttttgaacgc gaattagacc tgatgcgcgt ggataatctc ccgaattaac
     4261 ttaattaacg gcactcctca gcaaatataa tgaccctctt gataacccaa gagggcattt
     4321 tttaatgccc atggcgttta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     4381 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     4441 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     4501 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     4561 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     4621 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     4681 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     4741 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     4801 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     4861 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     4921 atgcggcgac cgagttgatc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     4981 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     5041 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     5101 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     5161 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     5221 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     5281 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     5341 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc cttaggccaa
     5401 ataggccgt
//
