LOCUS       pTW170b2                2594 bp ds-DNA     circular     20-FEB-2020
DEFINITION  [p15A] SD8 gVI kan.
ACCESSION   urn.local...q-b84xbed
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     modified_base   1..12
                     /created_by="tinawang"
                     /label="USER junction (Tm 49.2)"
     terminator      17..62
                     /note="rrnB1 transcriptional terminator***"
                     /note="Modded to remove elements that cause sigma70
                     binding sites to arise with new binding sites"
     misc_feature    complement(84..86)
                     /created_by="User"
                     /modified_by="shannonmiller"
                     /label="PAM"
     gBlock          <87..>106
                     /created_by="User"
                     /modified_by="User"
                     /label="gJH40"
     source          <87..>106
                     /dnas_title="pTW001a - AP SC101 Ptet SD8 gIV bla"
     source          complement(<87..>106)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(87..106)
                     /created_by="tinawang"
                     /label="s13"
     protein_binding 118..134
                     /label="OR2 protein bind"
     promoter        135..185
                     /modified_by="tinawang"
                     /label="lac promoter (no lacO no CAP)"
     misc_feature    135..141
                     /label="from CAP"
     misc_feature    148
                     /label="Misc_Feature_16"
     -35_signal      152..157
                     /label="-35 Signal"
     -10_signal      176..181
                     /label="-10 Signal"
     modified_base   192..206
                     /note="USER linker"
                     /modified_by="tinawang"
                     /label="USER junction"
     misc_feature    193..200
                     /note="SbfI"
                     /note="Keep unique in all constructs to switch out
                     promoter or RBS"
     misc_feature    200..204
                     /note="BsgI"
                     /note="Keep unique in all constructs to swap out RBS"
     RBS             207..221
                     /note="SD8 RBS (from Ringquist and Gold  Mol. Micro.
                     1992)"
                     /modified_by="tinawang"
                     /label="SD8"
     CDS             222..560
                     /created_by="tinawang"
                     /label="VI"
     modified_base   561..573
                     /note="USER linker"
                     /modified_by="tinawang"
                     /label="USER junction"
     terminator      575..608
                     /note="BBa_B1002"
     terminator      complement(610..648)
                     /note="BBa_B1006"
     modified_base   649..663
                     /created_by="tinawang"
                     /label="USER junction"
     misc_feature    complement(666..748)
                     /modified_by="jhu"
                     /label="gRNA backbone"
     gBlock          <749..>768
                     /created_by="User"
                     /modified_by="User"
                     /label="gJH40"
     source          <749..>768
                     /dnas_title="pTW001a - AP SC101 Ptet SD8 gIV bla"
     source          complement(<749..>768)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(749..768)
                     /created_by="tinawang"
                     /label="s13"
     misc_feature    complement(769..807)
                     /label="lac promoter (no LacO)"
                     /note="Geneious type: promoter prokaryotic"
     -10_signal      complement(775..780)
     -35_signal      799..804
     misc_feature    complement(823..837)
                     /label="CAP binding site"
     misc_feature    complement(853..945)
                     /label="LacI fragment"
     misc_feature    946..1005
                     /label="Misc_Feature_13"
     misc_feature    946..962
                     /label="USER linker"
     terminator      complement(963..996)
                     /label="BBa_B1002 terminator"
     misc_feature    992..>1038
                     /label="Misc_Feature_14"
     terminator      complement(997..1038)
                     /label="tetA/orfL bidirectional terminator"
     modified_base   1039..1051
                     /note="USER junction"
                     /modified_by="tinawang"
                     /label="USER junction"
     CDS             complement(1059..1874)
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /label="KanR"
     misc_feature    complement(1875..1925)
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /label="minimal promoter?"
     misc_feature    1891..1935
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /label="pr"
     modified_base   1926..1935
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /label="USER junction 22"
     misc_feature    1926..1947
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /label="pr"
     rep_origin      complement(1943..2569)
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /label="p15A Ori"
     promoter        2376..2415
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /label="RNA I Promoter (0.96)"
     -35_signal      2381..2386
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /label="-35 -35 signal"
     -10_signal      2405..2410
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /label="-10 -10 signal"
     promoter        complement(2527..2566)
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /label="RNA II Promoter (0.92)"
     -10_signal      complement(2532..2537)
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /label="-10 -10 signal"
     -35_signal      complement(2555..2560)
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /label="-35 -35 signal"
     misc_feature    2570..2594
                     /created_by="tinawang"
                     /modified_by="tinawang"
                     /label="from pBT38a"
ORIGIN      
        1 acgagaggac atccgggcaa atgcaacgaa aggctcagtc gaaagactgg gcctttcgtt
       61 gcatggaaag aagagaaacc tgattaagga aaagctgtcc tgcgactagg gaccctacaa
      121 cacgcacggt gttacattag gcaccccggg ctttacactt tatgcttccg gctcgtatgt
      181 tgtgtcgacc gacctgcagg tgcagtaagg aggaaaaaaa aatgccagtt cttttgggta
      241 ttccgttatt attgcgtttc ctcggtttcc ttctggtaac tttgttcggc tatctgctta
      301 cttttcttaa aaagggcttc ggtaagatag ctattgctat ttcattgttt cttgctctta
      361 ttattgggct taactcaatt cttgtgggtt atctctctga tattagcgct caattaccct
      421 ctgactttgt tcagggtgtt cagttaattc tcccgtctaa tgcgcttccc tgtttttatg
      481 ttattctctc tgtaaaggct gctattttca tttttgacgt taaacaaaaa atcgtttctt
      541 atttggattg ggataaataa acggagccaa tgtacgcaaa aaaccccgct tcagcggggt
      601 tttttcgcca aaaaaaaccc cgccctgtca ggggcggggt ttttttttag ggccctactg
      661 actgtaaaaa aagcaccgac tcggtgccac tttttcaagt tgataacgga ctagccttat
      721 tttaacttgc tatttctagc tctaaaacag gaaaagctgt cctgcgaccc acacaacata
      781 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
      841 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
      901 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgcactta attaacggca
      961 ctgcgaaaaa accccgccga agcggggttt tttgcgaaat ataatgaccc tcttgataac
     1021 ccaagagggc attttttaat gcccatggcg tacggatctt agaaaaactc atcgagcatc
     1081 aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt
     1141 ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat
     1201 cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa
     1261 ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa
     1321 agtttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa
     1381 tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg
     1441 cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact
     1501 gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct
     1561 gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc
     1621 ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta
     1681 acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc
     1741 ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac
     1801 ccatataaat cagcatccat gttggaattt aatcgcggcc tagagcaaga cgtttcccgt
     1861 tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt
     1921 catgaagaga aacctacgga tcggggcgga gatttcctgg aagatgccag gaagatactt
     1981 aacagggaag tgagagggcc gcggcaaagc cgtttttcca taggctccgc ccccctgaca
     2041 agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga ctataaagat
     2101 accaggcgtt tccccctggc ggctccctcg tgcgctctcc tgttcctgcc tttcggttta
     2161 ccggtgtcat tccgctgtta tggccgcgtt tgtctcattc cacgcctgac actcagttcc
     2221 gggtaggcag ttcgctccaa gctggactgt atgcacgaac cccccgttca gtccgaccgc
     2281 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg aaagacatgc aaaagcacca
     2341 ctggcagcag ccactggtaa ttgatttaga ggagttagtc ttgaagtcat gcgccggtta
     2401 aggctaaact gaaaggacaa gttttggtga ctgcgctcct ccaagccagt tacctcggtt
     2461 caaagagttg gtagctcaga gaaccttcga aaaaccgccc tgcaaggcgg ttttttcgtt
     2521 ttcagagcaa gagattacgc gcagaccaaa acgatctcaa gaagatcatc ttattaagga
     2581 gccccatacg atat
//
