LOCUS       Exported                4273 bp ds-DNA     circular SYN 22-NOV-2019
DEFINITION  [ColE1] G7' GAT sd5 g3C aadA.
ACCESSION   urn.local...5q-b89f66t
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Nov 22, 2019 from SnapGene Viewer 5.0.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(1..792)
                     /standard_name="aadA"
                     /label=aadA
     promoter        793..877
                     /note="aadA promoter"
     rep_origin      894..1481
     CDS             complement(1909..2100)
                     /label=Rop
                     /note="Rop"
     terminator      2211..2255
                     /note="rrnB1 transcriptional terminator"
     misc_feature    complement(2284..2286)
                     /standard_name="PAM"
                     /label=PAM
     misc_feature    complement(2287..2306)
                     /standard_name="protospacer G7'"
                     /label=protospacer G7'
     misc_feature    2318..2334
                     /standard_name="OR2 protein bind"
                     /label=OR2 protein bind
                     /note="Geneious type: protein_binding"
     misc_feature    2335..2385
                     /standard_name="lac promoter (no lacO no CAP)"
                     /label=lac promoter (no lacO no CAP)
     misc_feature    2335..2341
                     /standard_name="from CAP"
                     /label=from CAP
     misc_feature    2348
                     /standard_name="Misc_Feature_16"
                     /label=Misc_Feature_16
     -35_signal      2352..2357
                     /standard_name="-35 Signal"
     -10_signal      2376..2381
                     /standard_name="-10 Signal"
     RBS             2407..2420
                     /standard_name="sd5 (0.048)"
                     /label=sd5 (0.048)
     CDS             2421..2561
                     /standard_name="NpuC wt/g3p SP"
                     /label=NpuC wt/g3p SP
     gene            2421..2528
                     /standard_name="NpuC wt"
                     /label=NpuC wt
     misc_difference 2529..2537
                     /standard_name="extein scar"
                     /label=extein scar
     sig_peptide     2538..2561
                     /standard_name="g3p SP"
                     /label=g3p SP
     CDS             2562..3782
                     /standard_name="NpuC wt/g3p SP/g3p"
                     /label=NpuC wt/g3p SP/g3p
     misc_feature    3650..3695
                     /label=modified to remove internal promoter
                     /note="modified to remove internal promoter"
     terminator      3797..3830
                     /note="BBa_B1002"
     terminator      complement(3832..3870)
                     /note="BBa_B1006"
     misc_feature    complement(3888..3970)
                     /standard_name="gRNA backbone"
                     /label=gRNA backbone
     misc_feature    complement(3971..3990)
                     /standard_name="G7'"
                     /label=G7'
     misc_feature    complement(3991..4029)
                     /standard_name="lac promoter (no LacO)"
                     /label=lac promoter (no LacO)
                     /note="Geneious type: promoter prokaryotic"
     -10_signal      complement(3997..4002)
     -35_signal      4021..4026
     misc_feature    complement(4045..4059)
                     /standard_name="CAP binding site"
                     /label=CAP binding site
     misc_feature    complement(4075..4167)
                     /standard_name="LacI fragment"
                     /label=LacI fragment
     misc_feature    4168..4227
                     /standard_name="Misc_Feature_13"
                     /label=Misc_Feature_13
     misc_feature    4168..4184
                     /standard_name="USER linker"
                     /label=USER linker
     terminator      complement(4185..4218)
                     /standard_name="BBa_B1002 terminator"
                     /label=BBa_B1002 terminator
     misc_feature    4214..4260
                     /standard_name="Misc_Feature_14"
                     /label=Misc_Feature_14
     terminator      complement(4219..4260)
                     /standard_name="tetA/orfL bidirectional terminator"
                     /label=tetA/orfL bidirectional terminator
ORIGIN
        1 ttatttgccg actaccttgg tgatctcgcc tttcacgtag tggacaaatt cttccaactg
       61 atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga taagcctgtc tagcttcaag
      121 tatgacgggc tgatactggg ccggcaggcg ctccattgcc cagtcggcag cgacatcctt
      181 aggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt
      241 tcgctcatcg ccagcccagt cgggcggcga gttccatagc gttaaggttt catttagcgc
      301 ctcaaataga tcctgttcag gaaccggatc aaagagttcc tccgccgctg gacctaccaa
      361 ggcaacgcta tgttctcttg cttttgtcag caagatagcc agatcaatgt cgatcgtggc
      421 tggctcgaag atacctgcaa gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg
      481 cttagctgga taacgccaag gaatgatgtc gtcgtgcaca acaatggtga cttctacagc
      541 gcggagaatc tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc
      601 tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc agcaaatcaa tatcactgtg
      661 tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt acggccagca acgtcggttc
      721 gagatggcgc tcgatgacgc caactacctc tgatagttga gtcgatactt cggcgatcac
      781 cgcttccctc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
      841 catgagcgga tacatatttg aatgtattta gaaaaatagg ccaaataggc cgttgagatc
      901 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
      961 tttgtttgcc ggatcaagag ctaccacctc tttttccgaa ggtaactggc ttcagcagag
     1021 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     1081 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     1141 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     1201 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     1261 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     1321 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     1381 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     1441 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     1501 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     1561 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     1621 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
     1681 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct
     1741 gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat
     1801 ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     1861 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     1921 accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag
     1981 cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt
     2041 taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac
     2101 tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga
     2161 gaggatgctc acgatacggg ttactgatga tgaacaagag gacatccggt caaataaaac
     2221 gaaaggctca gtcgaaagac tgggcctttc gttttgctga ggatggaaag aagagaaacc
     2281 tgagggttcg ttttgctgag gagacttagg gaccctacaa cacgcacggt gttacattag
     2341 gcaccccggg ctttacactt tatgcttccg gctcgtatgt tgtgtcgacc gacctgcagg
     2401 tgcagtaaaa aaggaaaaaa atgatcaaaa ttgccacgcg taaatattta ggcaaacaga
     2461 atgtttatga tatcggtgtc gagcgcgatc ataatttcgc gctgaaaaac ggctttatcg
     2521 ccagcaattg ttttaatgtt gttcctttct attctcactc cgctgaaact gttgaaagtt
     2581 gtttagcaaa accccataca gaaaattcat ttactaacgt ctggaaagac gacaaaactt
     2641 tagatcgtta cgctaactat gagggctgtc tgtggaatgc tacaggcgtt gtagtttgta
     2701 ctggtgacga aactcagtgt tacggtacat gggttcctat tgggcttgct atccctgaaa
     2761 atgagggtgg tggctctgag ggtggcggtt ctgagggtgg cggttctgag ggtggcggta
     2821 ctaaacctcc tgagtacggt gatacaccta ttccgggcta tacttatatc aaccctctcg
     2881 acggcactta tccgcctggt actgagcaaa accccgctaa tcctaatcct tctcttgagg
     2941 agtctcagcc tcttaatact ttcatgtttc agaataatag gttccgaaat aggcaggggg
     3001 cattaactgt ttatacgggc actgttactc aaggcactga ccccgttaaa acttattacc
     3061 agtacactcc tgtatcatca aaagccatgt atgacgctta ctggaacggt aaattcagag
     3121 actgcgcttt ccattctggc tttaatgagg atccattcgt ttgtgaatat caaggccaat
     3181 cgtctgacct gcctcaacct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg
     3241 gcggctctga gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag
     3301 gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta
     3361 ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca
     3421 aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt
     3481 ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg
     3541 ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt
     3601 ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cgctggtaaa ccttacgagt
     3661 tcagtatcga ctgcgataag atcaacctgt tccgcggtgt ctttgcgttt cttttatatg
     3721 ttgccacctt tatgtatgta ttttctacgt ttgctaacat actgcgtaat aaggagtctt
     3781 aaacggagcc aatgtacgca aaaaaccccg cttcagcggg gttttttcgc caaaaaaaac
     3841 cccgccctgt caggggcggg gttttttttt agggccctac tgactgtaaa aaaagcaccg
     3901 actcggtgcc actttttcaa gttgataacg gactagcctt attttaactt gctatttcta
     3961 gctctaaaac ttcgttttgc tgaggagact ccacacaaca tacgagccgg aagcataaag
     4021 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     4081 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     4141 gggagaggcg gtttgcgtat tgggcgcact taattaacgg cactgcgaaa aaaccccgcc
     4201 gaagcggggt tttttgcgaa atataatgac cctcttgata acccaagagg gcatttttta
     4261 atgcccatgg cgt
//
