LOCUS       Exported                3820 bp ds-DNA     circular SYN 11-FEB-2020
DEFINITION  [pSC101] GAT sd8 g3N bla.
ACCESSION   urn.local...5r-b89f66t
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 11, 2020 from SnapGene 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(1..951)
                     /label=RepA
                     /note="RepA"
                     /note="pSC101 origin of replication protein"
     rep_origin      complement(955..1700)
                     /direction=LEFT
     terminator      1715..1760
                     /note="rrnB1 transcriptional terminator***"
                     /note="Modded to remove elements that cause sigma70
                     binding sites to arise with new binding sites"
     misc_feature    complement(1782..1784)
                     /standard_name="PAM"
                     /label=PAM
     misc_feature    complement(1785..1804)
                     /standard_name="Doench On 2 - from pJH451"
                     /label=Doench On 2 - from pJH451
     misc_feature    1816..1832
                     /standard_name="OR2 protein bind"
                     /label=OR2 protein bind
                     /note="Geneious type: protein_binding"
     misc_feature    1833..1883
                     /standard_name="lac promoter (no lacO no CAP)"
                     /label=lac promoter (no lacO no CAP)
     misc_feature    1833..1839
                     /standard_name="from CAP"
                     /label=from CAP
     misc_feature    1846
                     /standard_name="Misc_Feature_16"
                     /label=Misc_Feature_16
     -35_signal      1850..1855
                     /standard_name="-35 Signal"
     -10_signal      1874..1879
                     /standard_name="-10 Signal"
     misc_feature    1891..1898
                     /label=SbfI
                     /note="SbfI"
                     /note="Keep unique in all constructs to switch out
                     promoter or RBS"
     misc_feature    1898..1902
                     /label=BsgI
                     /note="BsgI"
                     /note="Keep unique in all constructs to swap out RBS"
     RBS             1905..1918
                     /standard_name="sd8 (0.2)"
                     /label=sd8 (0.2)
     CDS             1919..2257
                     /standard_name="g3P SP 1-10/NpuN wt"
                     /label=g3P SP 1-10/NpuN wt
     sig_peptide     1919..1948
                     /standard_name="g3p SP 1-10"
                     /label=g3p SP 1-10
     terminator      2272..2305
                     /note="BBa_B1002"
     terminator      complement(2307..2345)
                     /note="BBa_B1006"
     misc_feature    complement(2363..2445)
                     /standard_name="gRNA backbone"
                     /label=gRNA backbone
     misc_feature    complement(2446..2465)
                     /standard_name="Doench On 2"
                     /label=Doench On 2
     misc_feature    complement(2466..2504)
                     /standard_name="lac promoter (no LacO)"
                     /label=lac promoter (no LacO)
                     /note="Geneious type: promoter prokaryotic"
     -10_signal      complement(2472..2477)
     -35_signal      2496..2501
     misc_feature    complement(2520..2534)
                     /standard_name="CAP binding site"
                     /label=CAP binding site
     misc_feature    complement(2550..2642)
                     /standard_name="LacI fragment"
                     /label=LacI fragment
     misc_feature    2643..2702
                     /standard_name="Misc_Feature_13"
                     /label=Misc_Feature_13
     misc_feature    2643..2659
                     /standard_name="USER linker"
                     /label=USER linker
     terminator      complement(2660..2693)
                     /standard_name="BBa_B1002 terminator"
                     /label=BBa_B1002 terminator
     misc_feature    2689..2735
                     /standard_name="Misc_Feature_14"
                     /label=Misc_Feature_14
     terminator      complement(2694..2735)
                     /standard_name="tetA/orfL bidirectional terminator"
                     /label=tetA/orfL bidirectional terminator
     CDS             complement(2749..3609)
                     /label=bla
                     /note="bla"
                     /note="from pUC19"
     promoter        complement(3610..3804)
                     /note="bla promoter"
                     /note="from pUC19"
     misc_feature    3806..3818
                     /label=SfiI
                     /note="SfiI"
                     /note="Keep unique in all constructs, can be used to
                     exchange antibiotic resistance markers or plasmid origins"
ORIGIN
        1 tcagatcctt ccgtatttag ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag
       61 ccatgagaac gaaccattga gatcatgctt actttgcatg tcactcaaaa attttgcctc
      121 aaaactggtg agctgaattt ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt
      181 acgtaggtag gaatctgatg taatggttgt tggtattttg tcaccattca tttttatctg
      241 gttgttctca agttcggtta cgagatccat ttgtctatct agttcaactt ggaaaatcaa
      301 cgtatcagtc gggcggcctc gcttatcaac caccaatttc atattgctgt aagtgtttaa
      361 atctttactt attggtttca aaacccattg gttaagcctt ttaaactcat ggtagttatt
      421 ttcaagcatt aacatgaact taaattcatc aaggctaatc tctatatttg ccttgtgagt
      481 tttcttttgt gttagttctt ttaataacca ctcataaatc ctcatagagt atttgttttc
      541 aaaagactta acatgttcca gattatattt tatgaatttt tttaactgga aaagataagg
      601 caatatctct tcactaaaaa ctaattctaa tttttcgctt gagaacttgg catagtttgt
      661 ccactggaaa atctcaaagc ctttaaccaa aggattcctg atttccacag ttctcgtcat
      721 cagctctctg gttgctttag ctaatacacc ataagcattt tccctactga tgttcatcat
      781 ctgagcgtat tggttataag tgaacgatac cgtccgttct ttccttgtag ggttttcaat
      841 cgtggggttg agtagtgcca cacagcataa aattagcttg gtttcatgct ccgttaagtc
      901 atagcgacta atcgctagtt catttgcttt gaaaacaact aattcagaca tacatctcaa
      961 ttggtctagg taattttaat cactatacca attgagatgg gctagtcaat gataattact
     1021 agtccttttc ctttgagttg tgggtatctg taaattctgc tagacctttg ctggaaaact
     1081 tgtaaattct gctagaccct ctgtaaattc cgctagacct ttgtgtgttt tttttgttta
     1141 tattcaagtg gttataattt atagaataaa gaaagaataa aaaaagataa aaagaataga
     1201 tcccagccct gtgtataact cactacttta gtcagttccg cagtattaca aaaggatgtc
     1261 gcaaacgctg tttgctcctc tacaaaacag accttaaaac cctaaaggct taagtagcac
     1321 cctcgcaagc tcgggcaaat cgctgaatat tccttttgtc tccgaccatc aggcacctga
     1381 gtcgctgtct ttttcgtgac attcagttcg ctgcgctcac ggctctggca gtgaatgggg
     1441 gtaaatggca ctacaggcgc cttttatgga ttcatgcaag gaaactaccc ataatacaag
     1501 aaaagcccgt cacgggcttc tcagggcgtt ttatggcggg tctgctatgt ggtgctatct
     1561 gactttttgc tgttcagcag ttcctgccct ctgcttttcc agtctgacca cttcggctta
     1621 tcccgtgaca ggtcattcag actggctaat gcacccagta aggcagcggg ctcatcaaca
     1681 ggcttacccg tcgtacggtc aagaggacat ccgggcaaat gcaacgaaag gctcagtcga
     1741 aagactgggc ctttcgttgc atggaaagaa gagaaacctg accgccaggt actcggtggg
     1801 ctcctaggga ccctacaaca cgcacggtgt tacattaggc accccgggct ttacacttta
     1861 tgcttccggc tcgtatgttg tgtcgaccga cctgcaggtg cagtaaagga aaaaaaaaat
     1921 gaaaaaatta ttattcgcaa ttcctttatg tctcagctac gaaaccgaaa tcttgaccgt
     1981 cgaatatggt ctgctgccaa tcggcaagat tgttgaaaaa cgtattgaat gtacggtcta
     2041 ctcagtggat aacaacggca atatctacac ccagccggtg gcccagtggc atgaccgtgg
     2101 tgaacaggaa gtgttcgaat attgtctgga agacggatct ttaatccgtg ccacaaagga
     2161 tcacaaattt atgactgtag atggtcagat gctcccaatc gacgaaattt ttgaacgcga
     2221 attagacctg atgcgcgtgg ataatctccc gaattaaacg gagccaatgt acgcaaaaaa
     2281 ccccgcttca gcggggtttt ttcgccaaaa aaaaccccgc cctgtcaggg gcggggtttt
     2341 tttttagggc cctactgact gtaaaaaaag caccgactcg gtgccacttt ttcaagttga
     2401 taacggacta gccttatttt aacttgctat ttctagctct aaaacccagg tactcggtgg
     2461 gctccccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     2521 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     2581 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     2641 gcacttaatt aacggcactg cgaaaaaacc ccgccgaagc ggggtttttt gcgaaatata
     2701 atgaccctct tgataaccca agagggcatt ttttaatgcc catggcgttt accaatgctt
     2761 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     2821 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     2881 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     2941 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     3001 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     3061 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     3121 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     3181 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     3241 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     3301 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccagc
     3361 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     3421 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     3481 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     3541 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     3601 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     3661 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     3721 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     3781 aaataggcgt atcacgaggc ccttaggcca aataggccgt
//
