LOCUS       pUC19_-_T7_-_diySpike1_ 3719 bp ds-DNA     circular     09-OCT-2019
DEFINITION  .
KEYWORDS    "accession:addgene_50005_74677"                                    
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     primer_bind     complement(2769..2787)
                     /label="pBRforEco"
     CDS             join(2960..3719,1..101)
                     /label="AmpR"
     misc_feature    1273..1274
                     /label="LEFTSITE"
     primer_bind     complement(2365..2382)
                     /label="M13 Forward"
     primer_bind     complement(2590..2609)
                     /label="pRS-marker"
     Promoter        1305..1322
                     /label="T7"
     promoter        1184..1214
                     /label="lac promoter"
     primer_bind     complement(3178..3197)
                     /label="Amp-R"
     primer_bind     1014..1031
                     /label="L4440"
     primer_bind     2709..2731
                     /label="pGEX 3'"
     primer_bind     complement(2365..2381)
                     /label="M13 fwd"
     misc_feature    1323..2322
                     /label="diySpike1"
     primer_bind     1246..1262
                     /label="M13 rev"
     primer_bind     complement(2374..2396)
                     /label="M13/pUC Forward"
     primer_bind     761..780
                     /label="pBR322ori-F"
     protein_bind    1222..1238
                     /label="lac operator"
     Other           2323..2352
                     /label="Hardcoded PolyA"
     protein_bind    1148..1169
                     /label="CAP binding site"
     misc_feature    complement(2390..2391)
                     /label="RIGHTSIDE"
     primer_bind     1227..1249
                     /label="M13/pUC Reverse"
     rep_origin      272..860
                     /label="ori"
     misc_feature    1245..2322
                     /label="diySpike1_GBlock"
     promoter        2855..2959
                     /label="AmpR promoter"
     primer_bind     1246..1262
                     /label="M13 Reverse"
ORIGIN
        1 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
       61 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
      121 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
      181 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
      241 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
      301 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
      361 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
      421 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
      481 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
      541 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
      601 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
      661 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      721 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      781 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      841 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
      901 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
      961 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1021 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     1081 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     1141 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     1201 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     1261 accatgatta cgccaagctt gcatgcctgc aggtcgactc tagaTAATAC GACTCACTAT
     1321 AGGGGTTGCC TTGCAAGACT AGTTGCTTCA TACCGCGGCG CTGTCTAGGG CCGGGGATTT
     1381 CGGGAAGTGT AGCTCGCTTG GCGACCTCGA TAGTGGGCCC GACCAAAGGT CGGAGCCATA
     1441 GGGTCATACT CGAAACTGGG TCTTGACCGC GGTTACGGCG AGGAGGGGAG ACCACCTTTC
     1501 GGACCTTTAG CATTATAGTT ACTTAATCAT GATCTGTCGG ACGTGGGTGA GAGATGCTGT
     1561 GTCGAGACAA CTCGCGCCGA TCTGTACAGA AGCTCCGGAC TGCGTATCTC CTTGGATGGT
     1621 GGAGCTGGCG GTTCTCGTGA CAAGAACACA AGGGCGGATC GAGCTCTGGT AAAGTATGTG
     1681 CACTGGGGAC GATCGAGCCG TCTTGCCGCG TGACAAGCAA ACTACCACCG TAAGAAGCCT
     1741 TAACCGAGTC AACCCCACGG GGTTTCCTGT TAATCGCTAG CGGCATGTCT ATCTCGTCAG
     1801 CTAGCATGAG TCACACGGAA CACCTGATCA TGAGGGCGCC CAAAGCAAGT ACGGACGTTG
     1861 TATGATTAGA CTAGGGACCC GAAGCTACCG AGATACCATT TGTGCCGACG ACATTCAACC
     1921 GTATGTGGCA ATCTGAGGAC GCGTGTGTCT CATTATCTTC AAGTCGTTAT TAAGCGGAGC
     1981 GCTTTCTCGT TGCGGTTTAA AAGATCTCCC TCGCCGTCGG AATCTGGGCA GTCTAACATA
     2041 GTTATGGGGG CGGAGTGATG GGCAGAATCC ACCCACGCAC GTCTCCGGAC GGAACATACG
     2101 AGAGGCGTGT GCCTGTGGTG CATGACGGTA CTTTTGTGCG GCGCGGTATT CATGACCTAT
     2161 GCCGCGGAGC GATTCACCTG ACCCGACTGT CGATAGGAGA CCGGAGCGAC TTTTTGTTAC
     2221 ACATAAGCGA TGACTGTGAA CCGTGTGAGG GGGTCCTCCT ATTAGGTTAC AACATTATGA
     2281 TACTACGAGC GGTCGGCCAA GTTGAGTTCC TGTACCAAAA CCAAAAAAAA AAAAAAAAAA
     2341 AAAAAAAAAA AAAAGCTTga attcactggc cgtcgtttta caacgtcgtg actgggaaaa
     2401 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     2461 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     2521 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
     2581 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     2641 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     2701 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     2761 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     2821 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     2881 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     2941 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     3001 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     3061 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     3121 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     3181 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     3241 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     3301 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     3361 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     3421 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     3481 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     3541 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     3601 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     3661 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccct
//