LOCUS       pUC19_-_T7_-_diySpike2_ 3719 bp ds-DNA     circular     09-OCT-2019
DEFINITION  .
KEYWORDS    "accession:addgene_50005_74677"                                    
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     primer_bind     complement(2446..2462)
                     /label="M13 fwd"
     misc_feature    1326..2403
                     /label="diySpike2_GBlock"
     Promoter        1386..1403
                     /label="T7"
     Other           2404..2433
                     /label="Hardcoded PolyA"
     primer_bind     complement(3259..3278)
                     /label="Amp-R"
     misc_feature    complement(2471..2472)
                     /label="RIGHTSIDE"
     primer_bind     1308..1330
                     /label="M13/pUC Reverse"
     protein_bind    1229..1250
                     /label="CAP binding site"
     primer_bind     1095..1112
                     /label="L4440"
     misc_feature    1404..2403
                     /label="diySpike2"
     rep_origin      353..941
                     /label="ori"
     primer_bind     1327..1343
                     /label="M13 rev"
     CDS             join(3041..3719,1..182)
                     /label="AmpR"
     primer_bind     complement(2446..2463)
                     /label="M13 Forward"
     protein_bind    1303..1319
                     /label="lac operator"
     primer_bind     complement(2850..2868)
                     /label="pBRforEco"
     promoter        1265..1295
                     /label="lac promoter"
     primer_bind     complement(2671..2690)
                     /label="pRS-marker"
     primer_bind     842..861
                     /label="pBR322ori-F"
     primer_bind     1327..1343
                     /label="M13 Reverse"
     primer_bind     complement(2455..2477)
                     /label="M13/pUC Forward"
     promoter        2936..3040
                     /label="AmpR promoter"
     primer_bind     2790..2812
                     /label="pGEX 3'"
     misc_feature    1354..1355
                     /label="LEFTSITE"
ORIGIN
        1 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
       61 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
      121 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
      181 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
      241 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
      301 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
      361 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
      421 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
      481 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
      541 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
      601 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
      661 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
      721 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
      781 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
      841 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
      901 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
      961 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     1021 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     1081 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     1141 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     1201 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     1261 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     1321 ttcacacagg aaacagctat gaccatgatt acgccaagct tgcatgcctg caggtcgact
     1381 ctagaTAATA CGACTCACTA TAGGGAGCCA GGTAATGATC ATGCATTGGG TTAGGTGCTT
     1441 AGACATGGGG ATCGGCATCT AGGGGCGCCT AGTTCCTAGG GCTACTGCCA ATCCATACAG
     1501 GTAGTAAAGC ACCCGGGTCC GATGAACTTG GACGACGAAA ACTGACAGAC CGCGCTGCAC
     1561 GAGGTCGTTC GTCATGGTGT GCGAGGAGCT AGCCGCAGGA GACTGTGTGG CAGTAGTCAG
     1621 CGAACGGCCC CCGGCCCCTG CCGGACTAAA TCCCCCTACC TGATCGCCGG GGGCACTCGA
     1681 ACCATGACGA GCTGGCGGCG AGCGTACTCA CACGCTATAT GCGCTCTTAG AGCCCGTTTC
     1741 TGAAGCAAGA TTACAGTGGC GTCCGCTTCG ACTCGTAGTT CTATGGCAGT GTGGAGTTCG
     1801 GGGTGCGGCT CTCCCTTGCG CGCCTCTGTT ACAAACCCGC CACTGAGTGC CGGCCGACGG
     1861 TCGGGTTACG CAGGAAATTC ATGCGGAACG CTAATGCGCA TCCGTCGTGG CGAATATTCA
     1921 CAATGCCGGG TCGCTGATAC GTGCTGGTAC CACTTACGTT ATTTACGGGG CCTTTAGCTC
     1981 AAGAGCACGT GTTGTCTAGG GAAGCCTTCG CTTTCATCGA CGGACCCATA GCCGGGACAA
     2041 TTCTGCATGA TGTTACCCTT ACCCGCACTG CGAGTCTATG GCCTCTCTGA CGGGCCTACT
     2101 CGACTGTGGC GCAAAGGACC GTGGTCTAGG TCGTCAGGAG CCACGGATCG TCTTATGTGT
     2161 AGAGTCCCAG GTCGCCACTA ACTGACAGCC GCGTGGACAG CGGTCGGGGA AGCCGGTACT
     2221 GCGAATTTAT CGACGTTCGA TTCAGCGGAT ACTCGCGCTC AGCAGTGTGG TTAATCGACC
     2281 GCAGAGAACC GTCATGTATG ATCTCTGCTA GTACTTAAGC TCGCGGAGCG CAAGAAATAC
     2341 GGACTTGTTG ATTCCTGATA CATACGCTGG GTCCTAATCC GGCATTTTCC ATACCCACTT
     2401 TCAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAGCTTg aattcactgg ccgtcgtttt
     2461 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     2521 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     2581 gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     2641 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     2701 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     2761 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     2821 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     2881 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     2941 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     3001 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     3061 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     3121 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     3181 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     3241 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     3301 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     3361 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     3421 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     3481 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     3541 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     3601 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     3661 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggct
//