LOCUS       pUC19_-_T7_-_diySpike3_ 3719 bp ds-DNA     circular     18-SEP-2019
DEFINITION  .
KEYWORDS    "accession:addgene_50005_74677"                                    
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     primer_bind     1047..1064
                     /label="L4440"
     protein_bind    1255..1271
                     /label="lac operator"
     primer_bind     complement(2398..2414)
                     /label="M13 fwd"
     primer_bind     complement(3211..3230)
                     /label="Amp-R"
     primer_bind     complement(2802..2820)
                     /label="pBRforEco"
     primer_bind     2742..2764
                     /label="pGEX 3'"
     misc_feature    1306..1307
                     /label="LEFTSITE"
     misc_feature    1356..2355
                     /label="diySpike3"
     primer_bind     794..813
                     /label="pBR322ori-F"
     primer_bind     complement(2398..2415)
                     /label="M13 Forward"
     primer_bind     1260..1282
                     /label="M13/pUC Reverse"
     primer_bind     complement(2407..2429)
                     /label="M13/pUC Forward"
     promoter        2888..2992
                     /label="AmpR promoter"
     Other           2356..2385
                     /label="Hardcoded PolyA"
     Promoter        1338..1355
                     /label="T7"
     CDS             join(2993..3719,1..134)
                     /label="AmpR"
     primer_bind     complement(2623..2642)
                     /label="pRS-marker"
     primer_bind     1279..1295
                     /label="M13 Reverse"
     rep_origin      305..893
                     /label="ori"
     misc_feature    1278..2355
                     /label="diySpike3_GBlock"
     misc_feature    complement(2423..2424)
                     /label="RIGHTSIDE"
     protein_bind    1181..1202
                     /label="CAP binding site"
     promoter        1217..1247
                     /label="lac promoter"
     primer_bind     1279..1295
                     /label="M13 rev"
ORIGIN
        1 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
       61 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
      121 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
      181 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
      241 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
      301 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
      361 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
      421 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
      481 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
      541 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
      601 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
      661 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
      721 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
      781 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
      841 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
      901 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
      961 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     1021 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     1081 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     1141 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     1201 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     1261 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cttgcatgcc
     1321 tgcaggtcga ctctagaTAA TACGACTCAC TATAGGCTTC TACTTCCCAG CCACAAACGA
     1381 GAAGGGTGAG CATATTACTC AAAATGTTTT CTATCCCCCC CTAGGTGGAA CTTATATTTC
     1441 GAATTAACGA CAAACCGGGA CGGCTCACCC CTCCATTTCG ATACCCACCG CCGAGAGGCC
     1501 GACGTATGCG ATGGCTAGTT CCGCCCACAG ATTATAATAC CAAGTCGCGC GAGGGTACCT
     1561 TGATTTTAAA TGTTCCTCCT TTACGTTATG TGTGCGGCCA CACCGTGTCC GTGTTTCGGT
     1621 CACACTTTAG TGCTATGAGT AACGTACACA GTCGCGCAGT GCACGTATAG ATGGAGCCCT
     1681 TATGTTGTTA AAGAAGTGCA GTACGCTTGA TCGGTAGAGC GACAACTCCC CGAGTTAAGT
     1741 GGCGTCGACC GACGAGTTAC CGCCGATGGG TAAAAATCTT TACAAGCACT TCCGAATATT
     1801 AGGATAATGC GCTCTTTGGC AAAATAAATG TCGGTGCTCC GAGTGGCTAC TAGCCTTCCC
     1861 GAAGAGTGGT GAACTAGATT GTGCTTAATG CACGACCTGC TTAGGTCACT AGTATCGTCC
     1921 GACCTGGCGG GTTTCTCATA CCAGTCGGCG TACGCTGACT AGTCACTCAA AGAGTAGTAG
     1981 GGTAACTCTC GAGGCAATAA GGTATTTGTG CCAGGTGCAT CCTCGGTGGG ACTATCGGTT
     2041 GCCACTACCC ATAGTGATGT CGATGTTCCG TTAGCACTGT TCGAAACGTT TCTTCCGGGT
     2101 ATCGCCAACC ATTGGACGGA CAATAAACTT CGCAAGAGGA AGTGGTGGTG TCTTAGTGCA
     2161 CAAGGGCCGA TATTGATGGA ACTCAGGGTC GGTGGGCCTC TGCGCAGATT GTCAAGTTTC
     2221 TTCCCTATTC GGTAACTGGA AAAGCACGCA TTCAGCTTGA CAAATCTCGT ATTGAATACA
     2281 TTCGTTAGCA CTAGACGTCA TAATGCTGGG TGCTAGGTAA ATTACTTAAC TAGTGTGACG
     2341 ACCTGATCAG AGTCTAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAGCT Tgaattcact
     2401 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     2461 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     2521 ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac
     2581 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
     2641 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     2701 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     2761 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     2821 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     2881 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     2941 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     3001 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     3061 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     3121 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     3181 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     3241 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     3301 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     3361 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     3421 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     3481 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     3541 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     3601 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     3661 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggta
//