LOCUS       Exported                4213 bp ds-DNA     circular SYN 25-NOV-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 25, 2019 from SnapGene Viewer 5.0.6
            https://www.snapgene.com
COMMENT     LOCUS       pGGB000	3749 bp 	DNA	SYN

            DEFINITION  pGGB000

            TITLE       pGGB000

            KEYWORDS    

            SOURCE      

              ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="synthetic DNA construct"
                     /label=pGGB000
     promoter        131..159
                     /label=AmpR_promoter
     gene            201..1061
                     /label=Ampicillin
     CDS             201..1061
                     /codon_start=1
                     /label=ORF frame 3
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             1216..1835
                     /codon_start=1
                     /label=pBR322_origin
                     /translation="RSKDLLEILFFCA*SAACKQKNHRYQRWFVCRIKSYQLFFRR*LA
                     SAERRYQILFF*CSRS*ATTSRTL*HRLHTSLC*SCYQWLLPVAISRVLPGWTQDDSYR
                     IRRSGRAERGVRAHSPAWSERPTPN*DTYSVSYEKAPRFPKGERRTGIR*AAGSEQESA
                     RGSFQGETPGIFIVLSGFATSDLSVDFCDARQGGGAYGKTPAT"
     misc_feature    2141..2162
                     /label=GG_seq_F
                     /note="GG_seq_F"
     CDS             2144..2173
                     /codon_start=1
                     /label=lac_promoter
                     /translation="FTLYASGSYV"
     CDS             2212..2229
                     /codon_start=1
                     /label=Sp6_primer
                     /translation="I*VTL"
     CDS             2252..3679
                     /codon_start=1
                     /label=dTomato (variant)
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     misc_feature    2252..2272
                     /label=tdTomato_GG2_F
                     /note="tdTomato_GG2_F"
     CDS             2420..2473
                     /codon_start=1
                     /label=Q66M (mCherry)
                     /translation="FAWDILSPQFMYGSKAYV"
     CDS             2847..2870
                     /codon_start=1
                     /label=dsRed1_C_primer
                     /translation="SWTSPPTT"
     CDS             3573..3596
                     /codon_start=1
                     /label=dsRed1_C_primer
                     /translation="SWTSPPTT"
     misc_feature    complement(3660..3683)
                     /label=tdTomato_GG2_R
                     /note="tdTomato_GG2_R"
     misc_feature    3680..3778
                     /label=linker
                     /note="linker"
     misc_feature    3680..3701
                     /label=linker_GG2_F_v2
                     /note="linker_GG2_F_v2"
     misc_feature    complement(3761..3782)
                     /label=linker_GG2_R_v2
                     /note="linker_GG2_R_v2"
     CDS             complement(3800..3818)
                     /codon_start=1
                     /label=T7_promoter
                     /translation="*YDSL"
     CDS             3828..3971
                     /codon_start=1
                     /label=lacZ_a
                     /translation="RFTTS*LGKPWRYPT*SPCSTSPFRQLA**RRGPHRSPFPTVAQP
                     EWR"
     CDS             complement(3828..3850)
                     /codon_start=1
                     /label=M13_pUC_fwd_primer
                     /translation="PSHDVVK"
     misc_feature    complement(3895..3916)
                     /label=GG_seq_R
                     /note="GG_seq_R"
     CDS             4163..4185
                     /codon_start=1
                     /label=pGEX_3_primer
                     /translation="PGAACVR"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaaac aatggtgagc aagggcgagg aggtcatcaa
     2281 agagttcatg cgcttcaagg tgcgcatgga gggctccatg aacggccacg agttcgagat
     2341 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
     2401 caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttca tgtacggctc
     2461 caaggcgtac gtgaagcacc ccgccgacat ccccgattac aagaagctgt ccttccccga
     2521 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggtctggtga ccgtgaccca
     2581 ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt
     2641 cccccccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg
     2701 cctgtacccc cgcgacggcg tgctgaaggg cgagatccac caggccctga agctgaagga
     2761 cggcggccac tacctggtgg agttcaagac catctacatg gccaagaagc ccgtgcaact
     2821 gcccggctac tactacgtgg acaccaagct ggacatcacc tcccacaacg aggactacac
     2881 catcgtggaa cagtacgagc gctccgaggg ccgccaccac ctgttcctgg ggcatggcac
     2941 cggcagcacc ggcagcggca gctccggcac cgcctcctcc gaggacaaca acatggccgt
     3001 catcaaagag ttcatgcgct tcaaggtgcg catggagggc tccatgaacg gccacgagtt
     3061 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
     3121 ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc agttcatgta
     3181 cggctccaag gcgtacgtga agcaccccgc cgacatcccc gattacaaga agctgtcctt
     3241 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggtc tggtgaccgt
     3301 gacccaggac tcctccctgc aggacggcac gctgatctac aaggtgaaga tgcgcggcac
     3361 caacttcccc cccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctccac
     3421 cgagcgcctg tacccccgcg acggcgtgct gaagggcgag atccaccagg ccctgaagct
     3481 gaaggacggc ggccactacc tggtggagtt caagaccatc tacatggcca agaagcccgt
     3541 gcaactgccc ggctactact acgtggacac caagctggac atcacctccc acaacgagga
     3601 ctacaccatc gtggaacagt acgagcgctc cgagggccgc caccacctgt tcctgtacgg
     3661 catggacgag ctgtacaagg cagcggccgc ttcagggagt ggttccggaa gcggctctgg
     3721 atcgggctca gggagtggtt ccggcagcgg ctctggatcg gctgccgcag caattgctgg
     3781 cttgagaccg aattctcgcc ctatagtgag tcgtattacc tggccgtcgt tttacaacgt
     3841 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     3901 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     3961 ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     4021 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     4081 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     4141 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     4201 ccgaaacgcg cga
//
