LOCUS       Exported                3384 bp ds-DNA     circular SYN 25-NOV-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 25, 2019 from SnapGene Viewer 5.0.6
            https://www.snapgene.com
COMMENT     LOCUS       pGGC000	3749 bp 	DNA	SYN

            DEFINITION  pGGC000

            ACCESSION   

            KEYWORDS    

            SOURCE      

              ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="synthetic DNA construct"
                     /label=pGGC000
     promoter        131..159
                     /label=AmpR_promoter
     gene            201..1061
                     /label=Ampicillin
     CDS             201..1061
                     /codon_start=1
                     /label=ORF frame 3
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             1216..1835
                     /codon_start=1
                     /label=pBR322_origin
                     /translation="RSKDLLEILFFCA*SAACKQKNHRYQRWFVCRIKSYQLFFRR*LA
                     SAERRYQILFF*CSRS*ATTSRTL*HRLHTSLC*SCYQWLLPVAISRVLPGWTQDDSYR
                     IRRSGRAERGVRAHSPAWSERPTPN*DTYSVSYEKAPRFPKGERRTGIR*AAGSEQESA
                     RGSFQGETPGIFIVLSGFATSDLSVDFCDARQGGGAYGKTPAT"
     misc_feature    2141..2162
                     /label=GG_seq_F
                     /note="GG_seq_F"
     CDS             2144..2173
                     /codon_start=1
                     /label=lac_promoter
                     /translation="FTLYASGSYV"
     CDS             2212..2229
                     /codon_start=1
                     /label=Sp6_primer
                     /translation="I*VTL"
     gene            2254..2949
                     /label=mScarlet
                     /note="mScarlet"
     CDS             2254..2949
                     /codon_start=1
                     /label=ORF frame 2
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     gene            2254..2949
                     /label=mScarlet
                     /note="mScarlet"
     misc_feature    2254..2275
                     /label=mScarlet_GG3_F
                     /note="mScarlet_GG3_F"
     CDS             2281..2949
                     /codon_start=1
                     /label=mCherry (36 - 704)
                     /translation="KEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGP
                     LPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGGAVTVTQDTS
                     LEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKMALRLKDGG
                     RYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHSTGGMDELY
                     K"
     misc_feature    complement(2929..2949)
                     /label=mScarlet_GG3_R
                     /note="mScarlet_GG3_R"
     CDS             complement(2971..2989)
                     /codon_start=1
                     /label=T7_promoter
                     /translation="*YDSL"
     misc_feature    complement(2991..3015)
                     /label=M13F
                     /note="M13F"
     CDS             2999..3142
                     /codon_start=1
                     /label=lacZ_a
                     /translation="RFTTS*LGKPWRYPT*SPCSTSPFRQLA**RRGPHRSPFPTVAQP
                     EWR"
     CDS             complement(2999..3021)
                     /codon_start=1
                     /label=M13_pUC_fwd_primer
                     /translation="PSHDVVK"
     misc_feature    complement(3066..3087)
                     /label=GG_seq_R
                     /note="GG_seq_R"
     CDS             3334..3356
                     /codon_start=1
                     /label=pGEX_3_primer
                     /translation="PGAACVR"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaggc tccatggtga gcaagggcga ggcagtgatc
     2281 aaggagttca tgcggttcaa ggtgcacatg gagggctcca tgaacggcca cgagttcgag
     2341 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
     2401 accaagggtg gccccctgcc cttctcctgg gacatcctgt cccctcagtt catgtacggc
     2461 tccagggcct tcaccaagca ccccgccgac atccccgact actataagca gtccttcccc
     2521 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgccgt gaccgtgacc
     2581 caggacacct ccctggagga cggcaccctg atctacaagg tgaagctccg cggcaccaac
     2641 ttccctcctg acggccccgt aatgcagaag aagacaatgg gctgggaagc gtccaccgag
     2701 cggttgtacc ccgaggacgg cgtgctgaag ggcgacatta agatggccct gcgcctgaag
     2761 gacggcggcc gctacctggc ggacttcaag accacctaca aggccaagaa gcccgtgcag
     2821 atgcccggcg cctacaacgt cgaccgcaag ttggacatca cctcccacaa cgaggactac
     2881 accgtggtgg aacagtacga acgctccgag ggccgccact ccaccggcgg catggacgag
     2941 ctgtacaagt cagtgagacc gaattctcgc cctatagtga gtcgtattac ctggccgtcg
     3001 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     3061 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     3121 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
     3181 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     3241 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     3301 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     3361 caccgtcatc accgaaacgc gcga
//
