LOCUS       Exported                4138 bp ds-DNA     circular SYN 25-NOV-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 25, 2019 from SnapGene Viewer 5.0.6
            https://www.snapgene.com
COMMENT     LOCUS       pGGC000	3749 bp 	DNA	SYN

            DEFINITION  pGGC000

            ACCESSION   

            KEYWORDS    

            SOURCE      

              ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="synthetic DNA construct"
                     /label=pGGC000
     promoter        131..159
                     /label=AmpR_promoter
     gene            201..1061
                     /label=Ampicillin
     CDS             201..1061
                     /codon_start=1
                     /label=ORF frame 3
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             1216..1835
                     /codon_start=1
                     /label=pBR322_origin
                     /translation="RSKDLLEILFFCA*SAACKQKNHRYQRWFVCRIKSYQLFFRR*LA
                     SAERRYQILFF*CSRS*ATTSRTL*HRLHTSLC*SCYQWLLPVAISRVLPGWTQDDSYR
                     IRRSGRAERGVRAHSPAWSERPTPN*DTYSVSYEKAPRFPKGERRTGIR*AAGSEQESA
                     RGSFQGETPGIFIVLSGFATSDLSVDFCDARQGGGAYGKTPAT"
     CDS             2144..2173
                     /codon_start=1
                     /label=lac_promoter
                     /translation="FTLYASGSYV"
     CDS             2212..2229
                     /codon_start=1
                     /label=Sp6_primer
                     /translation="I*VTL"
     CDS             2252..3703
                     /label=XVE
     primer_bind     complement(2381..2400)
                     /label=XVE_3R Seq primer
     misc_feature    complement(3059..3079)
                     /label=XVE_R2
                     /note="XVE_R2"
     misc_feature    3076..3095
                     /label=XVE_F2
                     /note="XVE_F2"
     misc_feature    3083..3088
                     /label=ggtctc to ggactc
                     /note="ggtctc to ggactc"
     misc_feature    3114
                     /label=SNP
                     /note="58_49, 157, LBII"
     misc_feature    complement(3683..3703)
                     /label=XVE_GG3_R
                     /note="XVE_GG3_R"
     CDS             complement(3725..3743)
                     /codon_start=1
                     /label=T7_promoter
                     /translation="*YDSL"
     misc_feature    complement(3745..3769)
                     /label=M13F
                     /note="M13F"
     CDS             3753..3896
                     /codon_start=1
                     /label=lacZ_a
                     /translation="RFTTS*LGKPWRYPT*SPCSTSPFRQLA**RRGPHRSPFPTVAQP
                     EWR"
     CDS             complement(3753..3775)
                     /codon_start=1
                     /label=M13_pUC_fwd_primer
                     /translation="PSHDVVK"
     misc_feature    complement(3820..3841)
                     /label=GG_seq_R
                     /note="GG_seq_R"
     CDS             4088..4110
                     /codon_start=1
                     /label=pGEX_3_primer
                     /translation="PGAACVR"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaggc tatgaaagcg ttaacggcca ggcaacaaga
     2281 ggtgtttgat ctcatccgtg atcacatcag ccagacaggt atgccgccga cgcgtgcgga
     2341 aatcgcgcag cgtttggggt tccgttcccc aaacgcggct gaagaacatc tgaaggcgct
     2401 ggcacgcaaa ggcgttattg aaattgtttc cggcgcatca cgcgggattc gtctgttgca
     2461 ggaagaggaa gaagggttgc cgctggtagg tcgtgtggct gccggtgaac cgtcgagcgc
     2521 ccccccgacc gatgtcagcc tgggggacga gctccactta gacggcgagg acgtggcgat
     2581 ggcgcatgcc gacgcgctag acgatttcga tctggacatg ttgggggacg gggattcccc
     2641 gggtccggga tttacccccc acgactccgc cccctacggc gctctggata tggccgactt
     2701 cgagtttgag cagatgttta ccgatgccct tggaattgac gagtacggtg gggatccgtc
     2761 tgctggagac atgagagctg ccaacctttg gccaagcccg ctcatgatca aacgctctaa
     2821 gaagaacagc ctggccttgt ccctgacggc cgaccagatg gtcagtgcct tgttggatgc
     2881 tgagcccccc atactctatt ccgagtatga tcctaccaga cccttcagtg aagcttcgat
     2941 gatgggctta ctgaccaacc tggcagacag ggagctggtt cacatgatca actgggcgaa
     3001 gagggtgcca ggctttgtgg atttgaccct ccatgatcag gtccaccttc tagaatgtgc
     3061 ctggctagag atcctgatga ttggactcgt ctggcgctcc atggagcacc cagggaagct
     3121 actgtttgct cctaacttgc tcttggacag gaaccaggga aaatgtgtag agggcatggt
     3181 ggagatcttc gacatgctgc tggctacatc atctcggttc cgcatgatga atctgcaggg
     3241 agaggagttt gtgtgcctca aatctattat tttgcttaat tctggagtgt acacatttct
     3301 gtccagcacc ctgaagtctc tggaagagaa ggaccatatc caccgagtcc tggacaagat
     3361 cacagacact ttgatccacc tgatggccaa ggcaggcctg accctgcagc agcagcacca
     3421 gcggctggcc cagctcctcc tcatcctctc ccacatcagg cacatgagta acaaaggcat
     3481 ggagcatctg tacagcatga agtgcaagaa cgtggtgccc ctctatgacc tgctgctgga
     3541 gatgctggac gcccaccgcc tacatgcgcc cactagccgt ggaggggcat ccgtggagga
     3601 gacggaccaa agccacttgg ccactgcggg ctctacttca tcgcattcct tgcaaaagta
     3661 ttacatcacg ggggaggcag agggtttccc tgccacagtc tgatcagtga gaccgaattc
     3721 tcgccctata gtgagtcgta ttacctggcc gtcgttttac aacgtcgtga ctgggaaaac
     3781 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     3841 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     3901 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
     3961 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     4021 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     4081 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcga
//
