LOCUS       Exported                3507 bp ds-DNA     circular SYN 25-NOV-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 25, 2019 from SnapGene Viewer 5.0.6
            https://www.snapgene.com
COMMENT     LOCUS       pGGD000	3749 bp 	DNA	SYN

            DEFINITION  pGGD000

            TITLE       pGGD000

            KEYWORDS    

            SOURCE      

              ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="synthetic DNA construct"
                     /label=pGGD000
     promoter        131..159
                     /label=AmpR_promoter
     gene            201..1061
                     /label=Ampicillin
     CDS             201..1061
                     /codon_start=1
                     /label=ORF frame 3
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             1216..1835
                     /codon_start=1
                     /label=pBR322_origin
                     /translation="RSKDLLEILFFCA*SAACKQKNHRYQRWFVCRIKSYQLFFRR*LA
                     SAERRYQILFF*CSRS*ATTSRTL*HRLHTSLC*SCYQWLLPVAISRVLPGWTQDDSYR
                     IRRSGRAERGVRAHSPAWSERPTPN*DTYSVSYEKAPRFPKGERRTGIR*AAGSEQESA
                     RGSFQGETPGIFIVLSGFATSDLSVDFCDARQGGGAYGKTPAT"
     misc_feature    2141..2162
                     /label=GG_seq_F
                     /note="GG_seq_F"
     CDS             2144..2173
                     /codon_start=1
                     /label=lac_promoter
                     /translation="FTLYASGSYV"
     CDS             2212..2229
                     /codon_start=1
                     /label=Sp6_primer
                     /translation="I*VTL"
     misc_feature    2248..2275
                     /label=Linker_GG4_F
                     /note="Linker_GG4_F"
     misc_feature    2254..2352
                     /label=linker
                     /note="linker"
     misc_feature    2353..3072
                     /label=mVENUS CDS
                     /note="mVENUS CDS"
     misc_feature    complement(3049..3069)
                     /label=venus_GG3_R
                     /note="venus_GG3_R"
     CDS             complement(3094..3112)
                     /codon_start=1
                     /label=T7_promoter
                     /translation="*YDSL"
     CDS             3122..3265
                     /codon_start=1
                     /label=lacZ_a
                     /translation="RFTTS*LGKPWRYPT*SPCSTSPFRQLA**RRGPHRSPFPTVAQP
                     EWR"
     CDS             complement(3122..3144)
                     /codon_start=1
                     /label=M13_pUC_fwd_primer
                     /translation="PSHDVVK"
     misc_feature    complement(3189..3210)
                     /label=GG_seq_R
                     /note="GG_seq_R"
     CDS             3457..3479
                     /codon_start=1
                     /label=pGEX_3_primer
                     /translation="PGAACVR"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcatca ggcgcagcgg ccgcttcagg gagtggttcc
     2281 ggaagcggct ctggatcggg ctcagggagt ggttccggca gcggctctgg atcggctgcc
     2341 gcagcaattg ctatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     2401 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     2461 gatgccacct acggcaagct gaccctgaag ctgatctgca ccaccggcaa gctgcccgtg
     2521 ccctggccca ccctcgtgac caccctgggc tacggcctgc agtgcttcgc ccgctacccc
     2581 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     2641 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     2701 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     2761 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat caccgccgac
     2821 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcggc
     2881 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     2941 cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc
     3001 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     3061 ctgtacaagt aactgctgag accgaattct cgccctatag tgagtcgtat tacctggccg
     3121 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     3181 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     3241 aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc
     3301 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat
     3361 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     3421 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     3481 tttcaccgtc atcaccgaaa cgcgcga
//
