LOCUS       N                       5775 bp    DNA     circular     26-NOV-2019
Sequeneced on 04/22/13, OK. EGFP OK- order No.  10-223188117
FEATURES             Location/Qualifiers
     promoter        1..589
                     /dnas_title="CMV E promotor"
                     /vntifkey="29"
                     /label=CMV E promotor
     polyA_signal    2594..2644
                     /dnas_title="SV40 early PolyA"
                     /vntifkey="25"
                     /label=SV40 early PolyA
     rep_origin      2691..3146
                     /dnas_title="f1 ori"
                     /vntifkey="33"
                     /label=f1 ori
     promoter        3320..3588
                     /dnas_title="SV40 E promoter"
                     /vntifkey="29"
                     /label=SV40 E promoter
     promoter        3208..3243
                     /dnas_title="Bac promotor of Kan gene"
                     /vntifkey="29"
                     /label=Bac promotor of Kan gene
     promoter        3671..4199
                     /dnas_title="Kanamycin"
                     /vntifkey="29"
                     /label=Kanamycin
     rep_origin      5050..5693
                     /dnas_title="pUC ori"
                     /vntifkey="33"
                     /label=pUC ori
     polyA_signal    4701..4719
                     /dnas_title="HSV TK PolyA"
                     /vntifkey="25"
                     /label=HSV TK PolyA
     polyA_signal    2371..2444
                     /dnas_title="MCS"
                     /vntifkey="25"
                     /label=MCS
     misc_feature    1348..2370
                     /note="CREB1 1-341"
     PCR_primer      2374..2399
                     /dnas_title="TCCGGACTCAGATCTCGAGCTCAAGC"
                     /pair=""
                     /primer="TCCGGACTCAGATCTCGAGCTCAAGC"
                     /current=0
     CDS             613..1329
                     /dnas_title="mVenus"
                     /vntifkey="4"
                     /label=mVenus
     PCR_primer      1734..1754
                     /dnas_title="AAGGAGGCCTGCCTACAGGAA"
                     /pair=""
                     /primer="AAGGAGGCCTGCCTACAGGAA"
                     /current=0
     PCR_primer      complement(1711..1733)
                     /dnas_title="GAAAGGATTTCCCTTCGTTTTTG"
                     /pair=""
                     /primer="GAAAGGATTTCCCTTCGTTTTTG"
                     /current=1
     misc_feature    1744..1746
                     /note="s133a TCC/GCC"
     source          1..5775
                     /dnas_title="N terminal tagged CREB S133A"
ORIGIN      
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ctgatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgggc tacggcctgc agtgcttcgc ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat caccgccgac
     1081 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcggc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag ctaccagtcc aagctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagc ctacccctac ccctaccatg accatggact ctggagcaga caaccagcag
     1381 agtggagatg ctgctgtaac agaagctgaa agtcaacaaa tgacagttca agcccagcca
     1441 cagattgcca cattagccca ggtatccatg ccagcagctc atgcgacgtc atctgctccc
     1501 actgtaacct tagtgcagct gcccaatggg cagacagtcc aggtccatgg ggtcatccag
     1561 gcggcccagc catcagttat tcagtctcca caagtccaaa cagttcagtc ttcctgtaag
     1621 gacttaaaaa gacttttctc cggaactcag atttcaacta ttgcagaaag tgaagattca
     1681 caggagtctg tggatagtgt aactgattcc caaaaacgaa gggaaatcct ttcaaggagg
     1741 cctgcctaca ggaaaatttt gaatgactta tcttctgatg caccaggggt gccaaggatt
     1801 gaagaagaaa aatcagaaga agagacttca gcccctgcca tcaccactgt aacagtgcca
     1861 accccgattt accaaactag cagtgggcag tatattgcca ttacccaggg aggagcaata
     1921 cagctggcta acaatggtac cgatggggta cagggcctgc agacattaac catgaccaat
     1981 gcagctgcca ctcagccggg tactaccatt ctacaatatg cacagaccac tgatggacag
     2041 cagattctag tgcccagcaa ccaagttgtt gttcaagctg cctctggtga tgtacaaaca
     2101 taccagattc gcacagcacc cactagcacc attgcccctg gagttgttat ggcgtcctcc
     2161 ccagcacttc ctacacagcc tgctgaagaa gcagcacgaa agagagaggt tcgtctaatg
     2221 aagaacaggg aagcagcaag agaatgtcgt agaaagaaga aagaatatgt gaaatgttta
     2281 gagaacagag tggcagtgct tgaaaaccaa aacaaaacat tgattgagga gctaaaagca
     2341 cttaaggacc tttactgcca caagtcagat tgatccggac tcagatctcg agctcaagct
     2401 tcgaattctg cagtcgacgg taccgcgggc ccgggatcca ccggatctag ataactgatc
     2461 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     2521 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
     2581 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     2641 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaacgcgt aaattgtaag
     2701 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     2761 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     2821 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     2881 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     2941 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     3001 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     3061 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     3121 gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac
     3181 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     3241 ctgataaatg cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg
     3301 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     3361 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     3421 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     3481 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     3541 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     3601 tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg
     3661 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     3721 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     3781 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     3841 gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     3901 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     3961 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     4021 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     4081 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     4141 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag
     4201 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     4261 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     4321 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     4381 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     4441 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
     4501 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     4561 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     4621 gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa
     4681 ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt
     4741 gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc
     4801 cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt
     4861 gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac
     4921 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     4981 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     5041 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     5101 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     5161 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     5221 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     5281 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     5341 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     5401 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     5461 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     5521 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     5581 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     5641 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     5701 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     5761 attaccgcca tgcat
//

