LOCUS       Exported                5650 bp ds-DNA     circular SYN 27-NOV-2019
DEFINITION  mCherry-KIX acceptor for G-CREB.
ACCESSION   .
VERSION     .
KEYWORDS    CMV-mCherry-KIX-mCherry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5650)
  TITLE     In vivo imaging of the coupling between neuronal and CREB activity 
            in the mouse brain
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 27, 2019 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5650
                     /dnas_title="addgene-plasmid-137004 UPDATE"
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong
                     initiation of translation (Kozak, 1987)"
     CDS             613..1320
                     /dnas_title="monomeric derivative of DsRed fluorescent
                     protein (Shaner et al., 2004)"
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent
                     protein (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE
                     GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM
                     NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG
                     ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ
                     YERAEGRHSTGGMDELYK"
     CDS             1606..2316
                     /dnas_title="monomeric derivative of DsRed fluorescent
                     protein (Shaner et al., 2004)"
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent
                     protein (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE
                     GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM
                     NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG
                     ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ
                     YERAEGRHSTGGMDELYK"
     polyA_signal    2438..2559
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2475..2494)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2529..2548
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2566..3021)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2703..2724)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2915..2934
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3048..3152
                     /dnas_title="bla"
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3154..3511
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(3177..3197)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3362..3497
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3424..3443
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3546..4340
                     /dnas_title="aph(3')-II (or nptII)"
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and
                     G418 (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3600..3619)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4210..4229
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4528..4547)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4572..4619
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4948..5536
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     primer_bind     5437..5456
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    1771..1773
                     /note=""
     misc_feature    2143..2145
                     /note=""
     misc_feature    2215..2217
                     /note=""
     misc_feature    2263..2265
                     /note=""
     misc_feature    778..780
                     /note=""
     misc_feature    1150..1152
                     /note=""
     misc_feature    1222..1224
                     /note=""
     misc_feature    1270..1272
                     /note=""
ORIGIN      
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
      661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggC
      781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
      841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
      901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     1141 cactacgacg cCgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     1201 gcctacaacg tcaacatcaa gCtggacatc acctcccaca acgaggacta caccatcgtg
     1261 gaacagtacg aGcgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     1321 tccggactca gatctcgagc tcaagcttcg aattggcatg aacatgtgac tcaggaccta
     1381 cggagtcatc tagtccataa actcgttcaa gccatcttcc caactccaga ccctgcagct
     1441 ctgaaagatc gccgcatgga gaacctggtt gcctatgcta agaaagtgga gggagacatg
     1501 tatgagtctg ctaatagcag ggatgaatac tatcatttat tagcagagaa aatctataaa
     1561 atacaaaaat ctgcagtcga cggtaccgcg ggcccgggat ccggaatggt gagcaagggc
     1621 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
     1681 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     1741 acccagaccg ccaagctgaa ggtgaccaag ggCggccccc tgcccttcgc ctgggacatc
     1801 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
     1861 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     1921 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
     1981 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
     2041 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
     2101 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgcCgaggt caagaccacc
     2161 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagCtggac
     2221 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaGcgcgc cgagggccgc
     2281 cactccaccg gcggcatgga cgagctgtac aagtaagcgg ccgcgactct agatcataat
     2341 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     2401 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     2461 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     2521 ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta
     2581 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     2641 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     2701 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     2761 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     2821 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     2881 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     2941 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     3001 atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     3061 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     3121 aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa
     3181 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     3241 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     3301 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     3361 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     3421 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     3481 aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt
     3541 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     3601 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     3661 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     3721 aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     3781 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     3841 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     3901 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     3961 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     4021 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc
     4081 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     4141 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     4201 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     4261 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     4321 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     4381 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     4441 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     4501 cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac
     4561 ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca
     4621 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg
     4681 gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg
     4741 cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata
     4801 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     4861 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     4921 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     4981 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     5041 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     5101 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     5161 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     5221 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     5281 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     5341 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     5401 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     5461 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     5521 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     5581 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     5641 cgccatgcat
//

