LOCUS       Exported                5490 bp ds-DNA     circular SYN 27-NOV-2019
DEFINITION  AAV backbone, mEGFP-CREB donor for G-CREB.
ACCESSION   .
VERSION     .
KEYWORDS    AAV-SYN_mEGFP-CREB
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5490)
  TITLE     In vivo imaging of the coupling between neuronal and CREB activity 
            in the mouse brain
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5490)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 27, 2019 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5490
                     /dnas_title="addgene-plasmid-137006 UPDATE"
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        210..657
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific
                     expression (Kugler et al., 2003)"
     regulatory      682..691
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong
                     initiation of translation (Kozak, 1987)"
     CDS             688..1404
                     /dnas_title="enhanced GFP"
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(733..754)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(994..1013)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1341..1362
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     repeat_region   2753..2893
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
     repeat_region   2753..2882
                     /label=AAV2 ITR
     rep_origin      2968..3423
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3055..3074)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3265..3286
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3440..3459)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
     primer_bind     3559..3581
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3619..3637)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward
                     primer"
     promoter        3705..3809
                     /dnas_title="bla"
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3810..4670
                     /dnas_title="bla"
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     primer_bind     complement(4028..4047)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4841..5429
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     primer_bind     5330..5349
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtattacc gccatgcatt agttattaat agtaatcaat
      181 tacggggtct gcagagggcc ctgcgtatga gtgcaagtgg gttttaggac caggatgagg
      241 cggggtgggg gtgcctacct gacgaccgac cccgacccac tggacaagca cccaaccccc
      301 attccccaaa ttgcgcatcc cctatcagag agggggaggg gaaacaggat gcggcgaggc
      361 gcgtgcgcac tgccagcttc agcaccgcgg acagtgcctt cgcccccgcc tggcggcgcg
      421 cgccaccgcc gcctcagcac tgaaggcgcg ctgacgtcac tcgccggtcc cccgcaaact
      481 ccccttcccg gccaccttgg tcgcgtccgc gccgccgccg gcccagccgg accgcaccac
      541 gcgaggcgcg agataggggg gcacgggcgc gaccatctgc gctgcggcgc cggcgactca
      601 gcgctgcctc agtctgcggt gggcagcgga ggagtcgtgt cgtgcctgag agcgcagtcg
      661 aattggctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
      721 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      781 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
      841 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
      901 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      961 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     1021 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     1081 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     1141 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     1201 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     1261 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccAAGct gagcaaagac
     1321 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     1381 ctcggcatgg acgagctgta caagcctacc cctaccccta ccatgaccat ggactctgga
     1441 gcagacaacc agcagagtgg agatgctgct gtaacagaag ctgaaagtca acaaatgaca
     1501 gttcaagccc agccacagat tgccacatta gcccaggtat ccatgccagc agctcatgcg
     1561 acgtcatctg ctcccactgt aaccttagtg cagctgccca atgggcagac agtccaggtc
     1621 catggggtca tccaggcggc ccagccatca gttattcagt ctccacaagt ccaaacagtt
     1681 cagtcttcct gtaaggactt aaaaagactt ttctccggaa ctcagatttc aactattgca
     1741 gaaagtgaag attcacagga gtctgtggat agtgtaactg attcccaaaa acgaagggaa
     1801 atcctttcaa ggaggccttc ctacaggaaa attttgaatg acttatcttc tgatgcacca
     1861 ggggtgccaa ggattgaaga agaaaaatca gaagaagaga cttcagcccc tgccatcacc
     1921 actgtaacag tgccaacccc gatttaccaa actagcagtg ggcagtatat tgccattacc
     1981 cagggaggag caatacagct ggctaacaat ggtaccgatg gggtacaggg cctgcagaca
     2041 ttaaccatga ccaatgcagc tgccactcag ccgggtacta ccattctaca atatgcacag
     2101 accactgatg gacagcagat tctagtgccc agcaaccaag ttgttgttca agctgcctct
     2161 ggtgatgtac aaacatacca gattcgcaca gcacccacta gcaccattgc ccctggagtt
     2221 gttatggcgt cctccccagc acttcctaca cagcctgctg aagaagcagc acgaaagaga
     2281 gaggttcgtc taatgaagaa cagggaagca gcaagagaat gtcgtagaaa gaagaaagaa
     2341 tatgtgaaat gtttagagaa cagagtggca gtgcttgaaa accaaaacaa aacattgatt
     2401 gaggagctaa aagcacttaa ggacctttac tgccacaagt cagattaaaa gcttgtggca
     2461 tccctgtgac ccctccccag tgcctctcct ggccctggaa gttgccactc cagtgcccac
     2521 cagccttgtc ctaataaaat taagttgcat cattttgtct gactaggtgt ccttctataa
     2581 tattatgggg tggagggggg tggtatggag caaggggcaa gttgggaaga caacctgtag
     2641 ggcctgcggg gtctattggg aacccaaatt gctgggatta caggcgtgaa ccactgctcc
     2701 cttccctgtc cttctgattt tgtaggtaac cacgtgcgga ccgagcggcc gcaggaaccc
     2761 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga
     2821 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc
     2881 agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     2941 caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
     3001 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     3061 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     3121 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     3181 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     3241 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     3301 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     3361 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
     3421 ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     3481 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     3541 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     3601 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     3661 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     3721 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     3781 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     3841 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     3901 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     3961 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     4021 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     4081 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     4141 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     4201 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     4261 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     4321 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     4381 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     4441 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     4501 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     4561 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     4621 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     4681 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     4741 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     4801 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     4861 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     4921 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     4981 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     5041 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     5101 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     5161 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     5221 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     5281 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     5341 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     5401 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     5461 tggccttttg ctggcctttt gctcacatgt
//

