LOCUS       SynapsinP-mMaroo        5498 bp    DNA     circular     26-NOV-2019
Vector pAAV-mG-CREB cut with NheI, HindIII 
Insert KIX-MAR-KIX C1 with PCR - Gibson.  
Sequenced on 4.14.17- clone 5 is maxi preped and processed on.
FEATURES             Location/Qualifiers
     promoter        4826..5493
                     /dnas_title="pUC origin"
                     /vntifkey="29"
                     /label=pUC origin
     promoter        3818..4675
                     /dnas_title="Amp"
                     /vntifkey="29"
                     /label=Amp
     promoter        2993..3293
                     /dnas_title="f1 origin"
                     /vntifkey="29"
                     /label=f1 origin
     misc_feature    1..130
                     /dnas_title="L-ITR"
                     /vntifkey="21"
                     /label=L-ITR
     misc_feature    2761..2901
                     /dnas_title="R-ITR"
                     /vntifkey="21"
                     /label=R-ITR
     polyA_signal    2463..2721
                     /dnas_title="hGH polyA"
                     /vntifkey="25"
                     /label=hGH polyA
     promoter        195..659
                     /dnas_title="Synapsin promoter"
                     /vntifkey="29"
                     /label=Synapsin promoter
     misc_feature    687..1418
                     /note="mMaroon0.9"
     misc_feature    1452..1667
                     /note="KIX domain (mouse CBP, 577-679)"
     PCR_primer      complement(1429..1451)
                     /dnas_title="ATTCGAAGCTTGAGCTCGAGATC"
                     /pair=""
                     /primer="ATTCGAAGCTTGAGCTCGAGATC"
                     /current=0
     PCR_primer      1430..1466
                     /dnas_title="ATCTCGAGCTCAAGCTTCGAATTGGCATGAACATGTG"
                     /pair=""
                     /primer="ATCTCGAGCTCAAGCTTCGAATTGGCATGAACATGTG"
                     /current=0
     misc_feature    1653..1655
                     /note="essential Y"
     misc_feature    1665..1667
                     /note="essential K"
     misc_feature    1704..2435
                     /note="mMaroon0.9"
     PCR_primer      complement(1679..1703)
                     /dnas_title="TCCGGATCCCGGGCCCGCGGTACCG"
                     /pair=""
                     /primer="TCCGGATCCCGGGCCCGCGGTACCG"
                     /current=0
     PCR_primer      1684..1727
                     /dnas_title="CCGCGGGCCCGGGATCCGGAATGGTGAGCAAGGGCGAGGAGCTG"
                     /pair=""
                     /primer="CCGCGGGCCCGGGATCCGGAATGGTGAGCAAGGGCGAGGAGCTG"
                     /current=0
     misc_feature    667..686
                     /note="5 prime overhang"
     PCR_primer      645..686
                     /dnas_title="CCTGAGAGCGCAGTCGAATTGGTAGCGCTACCGGTCGCCACC"
                     /pair=""
                     /primer="CCTGAGAGCGCAGTCGAATTGGTAGCGCTACCGGTCGCCACC"
                     /current=0
     misc_feature    2436..2457
                     /note="3 overhang"
     PCR_primer      complement(2436..2478)
                     /dnas_title="GTCACAGGGATGCCACAAGCTTATGGCTGATTATGATCAGTTA"
                     /pair=""
                     /primer="GTCACAGGGATGCCACAAGCTTATGGCTGATTATGATCAGTTA"
                     /current=1
     source          1..5498
                     /dnas_title="SynapsinP-mMaroon-KIX-mMaroon _pAAV"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtattacc gccatgcatt agttattaat agtaatcaat
      181 tacggggtct gcagagggcc ctgcgtatga gtgcaagtgg gttttaggac caggatgagg
      241 cggggtgggg gtgcctacct gacgaccgac cccgacccac tggacaagca cccaaccccc
      301 attccccaaa ttgcgcatcc cctatcagag agggggaggg gaaacaggat gcggcgaggc
      361 gcgtgcgcac tgccagcttc agcaccgcgg acagtgcctt cgcccccgcc tggcggcgcg
      421 cgccaccgcc gcctcagcac tgaaggcgcg ctgacgtcac tcgccggtcc cccgcaaact
      481 ccccttcccg gccaccttgg tcgcgtccgc gccgccgccg gcccagccgg accgcaccac
      541 gcgaggcgcg agataggggg gcacgggcgc gaccatctgc gctgcggcgc cggcgactca
      601 gcgctgcctc agtctgcggt gggcagcgga ggagtcgtgt cgtgcctgag agcgcagtcg
      661 aattggtagc gctaccggtc gccaccatgg tgagcaaggg cgaggagctg atcaaggaga
      721 acatgcacac caagctgtac ctgacaggca ccgtgaacaa ccactacttc gaatgcaccg
      781 ccgaagggga gggcaagccc tacgagggca cccagaccaa caggatcaag gttgtgcgcg
      841 gaggccccct gccgttcgca ttcgacatcc tggccccctg ctttatgtac gggagcaaga
      901 ccttcattaa ccatccacct gacattcccg attattttaa gcagtccttc cctgagggct
      961 tcacatggga gagaaccacc gtttacgaag acgggggcac actcactgct acacaggaca
     1021 ccagcctcca ggacggctgc ctgatctaca acgtccaagt cagaggggag aacttcccat
     1081 ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggccagtacc gagaccctgt
     1141 accccgctga cggcagcctg gaaggcagac tggactgggc cctgaagctc gtgggcgggg
     1201 gccacctgca ctgcagactg gaaaccacat acagatccaa gaaacccgca aagaacctca
     1261 agatgcccgg cgtctatttt attgacagga gactggaaag aatcaaggag gccgacaatg
     1321 agacctacgt cgagcagcac gaggtggctg tggccagata ctgcgacctc cctagcaaac
     1381 tggggcacaa acttaatggc atggacgagc tgtacaagtc cggactcaga tctcgagctc
     1441 aagcttcgaa ttggcatgaa catgtgactc aggacctacg gagtcatcta gtccataaac
     1501 tcgttcaagc catcttccca actccagacc ctgcagctct gaaagatcgc cgcatggaga
     1561 acctggttgc ctatgctaag aaagtggagg gagacatgta tgagtctgct aatagcaggg
     1621 atgaatacta tcatttatta gcagagaaaa tctataaaat acaaaaatct gcagtcgacg
     1681 gtaccgcggg cccgggatcc ggaatggtga gcaagggcga ggagctgatc aaggagaaca
     1741 tgcacaccaa gctgtacctg acaggcaccg tgaacaacca ctacttcgaa tgcaccgccg
     1801 aaggggaggg caagccctac gagggcaccc agaccaacag gatcaaggtt gtgcgcggag
     1861 gccccctgcc gttcgcattc gacatcctgg ccccctgctt tatgtacggg agcaagacct
     1921 tcattaacca tccacctgac attcccgatt attttaagca gtccttccct gagggcttca
     1981 catgggagag aaccaccgtt tacgaagacg ggggcacact cactgctaca caggacacca
     2041 gcctccagga cggctgcctg atctacaacg tccaagtcag aggggagaac ttcccatcca
     2101 acggccctgt gatgcagaag aaaacactcg gctgggaggc cagtaccgag accctgtacc
     2161 ccgctgacgg cagcctggaa ggcagactgg actgggccct gaagctcgtg ggcgggggcc
     2221 acctgcactg cagactggaa accacataca gatccaagaa acccgcaaag aacctcaaga
     2281 tgcccggcgt ctattttatt gacaggagac tggaaagaat caaggaggcc gacaatgaga
     2341 cctacgtcga gcagcacgag gtggctgtgg ccagatactg cgacctccct agcaaactgg
     2401 ggcacaaact taatggcatg gacgagctgt acaagtaact gatcataatc agccataagc
     2461 ttgtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca
     2521 gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc
     2581 ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca
     2641 acctgtaggg cctgcggggt ctattgggaa cccaaattgc tgggattaca ggcgtgaacc
     2701 actgctccct tccctgtcct tctgattttg taggtaacca cgtgcggacc gagcggccgc
     2761 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg
     2821 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc
     2881 gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg
     2941 gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag
     3001 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     3061 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     3121 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     3181 aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     3241 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     3301 actcaaccct atctcgggct attcttttga tttataaggg attttgccga tttcggccta
     3361 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     3421 gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     3481 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     3541 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     3601 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     3661 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     3721 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     3781 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     3841 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     3901 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     3961 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     4021 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     4081 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     4141 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     4201 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     4261 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     4321 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     4381 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     4441 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     4501 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     4561 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     4621 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     4681 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     4741 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     4801 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     4861 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     4921 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     4981 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     5041 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     5101 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     5161 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     5221 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     5281 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     5341 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     5401 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     5461 acggttcctg gccttttgct ggccttttgc tcacatgt
//

