LOCUS       GFPo_GLO_SEC_3xF       10485 bp ds-DNA     circular     14-APR-2015
DEFINITION  Cloning vector pUC19, complete sequence.
KEYWORDS    .
SOURCE      Cloning vector pUC19
  ORGANISM  Cloning vector pUC19 other sequences; artificial sequences;
            vectors.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   New England Biolabs.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2007) Research Department, New England Biolabs,
            240 County Road, Ipswich, MA 01938, USA
COMMENT     To prepare vector for cloning: 
COMMENT     -Digest with ClaI and SpeI to fuse 5' homology arm directly to mNG,
            or 
COMMENT     -Digest with AvrII and SpeI to include a flexible linker (for
            C-terminal tags).
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    404..1010
                     /label=This sequence will be replaced by the homology arm
                     /ApEinfo_fwdcolor=#cc9966
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     -10_signal      complement(8313..8318)
                     /note="Plac promoter (drives lacZalpha;
                     counter-clockwise); TATGTT"
                     /label=Plac promoter (drives lacZalpha; counter-clockwise
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             join(2776..2916,2964..3797)
                     /label=SQT-1
                     /ApEinfo_fwdcolor=#00cccc
                     /ApEinfo_revcolor=#006666
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             6106..7131
                     /note="hygromycinR"
                     /label=hygR
                     /ApEinfo_fwdcolor=#006666
                     /ApEinfo_revcolor=#006666
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     -35_signal      complement(8337..8342)
                     /note="Plac promoter (drives lacZalpha;
                     counter-clockwise); TTTACA"
                     /label=Plac promoter (drives lacZalpha;
                     counter-clockwise(1)
                     /ApEinfo_label=Plac promoter (drives lacZalpha;
                     counter-clockwise
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1992..2021
                     /label=myc
                     /ApEinfo_fwdcolor=#6666ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     protein_bind    complement(8362..8374)
                     /note="CAP protein binding site"
                     /bound_moiety="catabolite activator protein (CAP)"
                     /label=CAP protein binding site
                     /ApEinfo_fwdcolor=#990099
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             join(1038..1214,1266..1419,1471..1634,1686..1910)
                     /label=GFP (new)
                     /ApEinfo_fwdcolor=#33cc66
                     /ApEinfo_revcolor=#006666
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_RNA        complement(8666..9218)
                     /note="RNAII transcript (complementary strand)"
                     /label=RNAII transcript (complementary strand)
                     /ApEinfo_fwdcolor=#cccc33
                     /ApEinfo_revcolor=#999933
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_RNA        9108..9215
                     /note="RNAI transcript"
                     /label=RNAI transcript
                     /ApEinfo_fwdcolor=#cccc33
                     /ApEinfo_revcolor=#999933
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(join(4170..4411,4463..4833,4885..5300))
                     /label=Cre
                     /ApEinfo_fwdcolor=#6699cc
                     /ApEinfo_revcolor=#006666
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      complement(8666..9254)
                     /note="pMB1 origin of replication (counter-clockwise)
                     (RNAII -35 to RNA/DNA switch point)"
                     /label=pMB1 origin of replication (counter-clockwise) (RN
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     -35_signal      9072..9077
                     /note="RNAI promoter (clockwise); TTGAAG"
                     /label=RNAI promoter (clockwise); TTGAAG
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     -35_signal      complement(9249..9254)
                     /note="RNAII promoter (counter-clockwise); TTGAGA"
                     /label=RNAII promoter (counter-clockwise); TTGAGA
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     intron          1938..1948
                     /label=intron
                     /ApEinfo_fwdcolor=#cc66ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     -10_signal      9094..9099
                     /note="RNAI promoter (clockwise); GCTACA"
                     /label=RNAI promoter (clockwise); GCTACA
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1949..1982
                     /label=loxP
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     -10_signal      complement(9228..9233)
                     /note="RNAII promoter (counter-clockwise); CGTAAT"
                     /label=RNAII promoter (counter-clockwise); CGTAAT
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     -10_signal      complement(10329..10334)
                     /note="bla promoter (counter-clockwise); GAGACA"
                     /label=bla promoter (counter-clockwise); GAGACA
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(9428..10087)
                     /label=AmpR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1989..1991
                     /label=spacer
                     /ApEinfo_fwdcolor=#cccccc
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_difference 3027..3027
                     /label=e1350
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(9425..10285)
                     /gene="bla"
                     /note="ampR (confers resistance to ampicillin)"
                     /codon_start=1
                     /product="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /label=beta-lactamase
                     /ApEinfo_fwdcolor=#00cccc
                     /ApEinfo_revcolor=#006666
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_binding    complement(8286..8308)
                     /label=LacO
                     /ApEinfo_fwdcolor=#6495ed
                     /ApEinfo_revcolor=#6495ed
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     terminator      2025..2405
                     /label=let-858 Terminator
                     /ApEinfo_fwdcolor=#cc0000
                     /ApEinfo_revcolor=#cc0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     intron          1983..1988
                     /label=intron(1)
                     /ApEinfo_label=intron
                     /ApEinfo_fwdcolor=#cc66ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     -35_signal      complement(10350..10355)
                     /note="bla promoter (counter-clockwise); TTCAAA"
                     /label=bla promoter (counter-clockwise); TTCAAA
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(join(146..395,8260..8268))
                     /label=LacZalpha
                     /ApEinfo_fwdcolor=#00cccc
                     /ApEinfo_revcolor=#006666
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(8260..8280)
                     /label=M13-rev
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     5'UTR           join(2690..2717,2774..2775)
                     /label=sqt-1 5'UTR
                     /ApEinfo_fwdcolor=#ccffcc
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_signal     1911..1931
                     /label=TEV site
                     /ApEinfo_fwdcolor=#ff6600
                     /ApEinfo_revcolor=#ff6600
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    661..966
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
                     /label=ccdB
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      complement(8648..9330)
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     3'UTR           3798..3834
                     /label=sqt-1 3'UTR
                     /ApEinfo_fwdcolor=#cc3333
                     /ApEinfo_revcolor=#cc3333
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        complement(5301..5700)
                     /label=hsp16-41 promoter
                     /ApEinfo_fwdcolor=#0080c0
                     /ApEinfo_revcolor=#0080c0
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     protein_bind    7615..7641
                     /label=Flag
                     /ApEinfo_fwdcolor=#ff6699
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        2406..2775
                     /label=Psqt1
                     /ApEinfo_fwdcolor=#33cc00
                     /ApEinfo_revcolor=#33cc00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             754..963
                     /label=ccdB(1)
                     /ApEinfo_label=ccdB
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     protein_bind    7588..7614
                     /label=Flag(1)
                     /ApEinfo_label=Flag
                     /ApEinfo_fwdcolor=#ff3366
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7549..7560
                     /label=spacer(1)
                     /ApEinfo_label=spacer
                     /ApEinfo_fwdcolor=#cccccc
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     protein_bind    7561..7587
                     /label=Flag(2)
                     /ApEinfo_label=Flag
                     /ApEinfo_fwdcolor=#cc3366
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     3'UTR           complement(3835..4166)
                     /label=tbb-2 3'UTR
                     /ApEinfo_fwdcolor=#cc3333
                     /ApEinfo_revcolor=#993333
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     intron          7543..7548
                     /label=intron(2)
                     /ApEinfo_label=intron
                     /ApEinfo_fwdcolor=#cc66ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     3'UTR           7157..7508
                     /label=unc-54 3'UTR
                     /ApEinfo_fwdcolor=#990000
                     /ApEinfo_revcolor=#cc0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7509..7542
                     /label=loxP(1)
                     /ApEinfo_label=loxP
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        5701..6063
                     /note="Prps-0"
                     /note="/ApEinfo_fwdcolor=pink"
                     /note="/ApEinfo_revcolor=pink; /ApEinfo_fwdcolor=pink;
                     /ApEinfo_revcolor=pink; /ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} width 5 offset 0"
                     /label=Prps-0
                     /ApEinfo_fwdcolor=#339933
                     /ApEinfo_revcolor=#339933
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8249..8255
                     /label=This sequence will be deleted if ClaI is used
                     /ApEinfo_fwdcolor=#ffcc99
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7642..8248
                     /label=This sequence will be replaced by the homology
                     arm(1)
                     /ApEinfo_label=This sequence will be replaced by the
                     homology arm
                     /ApEinfo_fwdcolor=#cc9966
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(7686..7991)
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
                     /label=ccdB(2)
                     /ApEinfo_label=ccdB
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(7689..7898)
                     /label=ccdB(3)
                     /ApEinfo_label=ccdB
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_structure  1011..1037
                     /label=Flexible Linker (will be deleted if ClaI is used)
                     /ApEinfo_fwdcolor=#999999
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     378..395
                     /label=M13-fwd
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtcgccg gcactagtTA CCCGAAGTAT
      421 GTCAAAAAGA GGTATGCTAT GAAGCAGCGT ATTACAGTGA CAGTTGACAG CGACAGCTAT
      481 CAGTTGCTCA AGGCATATAT GATGTCAATA TCTCCGGTTC GGTAAGCACA ACCATGCAGA
      541 ATGAAGCCCG TCGTCTGCGT GCCGAACGCT GGAAAGCGGA AAATCAGGAA GGGATGGCTG
      601 AGGTCGCCCG GTTTATTGAA ATGAACGGCT CTTTTGCCGA CGAGAACAGG GGCTGGTGAA
      661 ATGCAGTTTA AGGTTTACAC CTATAAAAGA GAGAGCCGTT ATCGTCTGTT TGTGGATGTA
      721 CAGAGTGATA TTATTGACAC GCCCGGGCGA CGGATGGTGA TCCCCCTGGC CAGTGCACGT
      781 CTGCTGTCAG ATAAAGTCCC CCGTGAACTT TACCCGGTGG TGCATATCGG GGATGAAAGC
      841 TGGCGCATGA TGACCACCGA TATGGCCAGT GTGCCGGTCT CCGTTATCGG GGAAGAAGTG
      901 GCTGATCTCA GCCACCGCGA AAATGACATC AAAAACGCCA TTAACCTGAT GTTCTGGGGA
      961 ATATAAATGT CAGGCTCCCT TATACACAGC CAGTCTGCAG GTCGAcctag GGAGCATCGG
     1021 GAGCCTCAGG AGCATCGATG CGTTCTTCCA AAGGAGAAGA ACTCTTTACC GGAGTCGTCC
     1081 CAATTCTCGT CGAGCTCGAC GGAGACGTAA ACGGACACAA ATTCTCGGTT TCCGGAGAAG
     1141 GAGAAGGAGA TGCTACTTAT GGAAAACTCA CCCTTAAATT CATTTGCACC ACCGGAAAGC
     1201 TCCCAGTGCC GTGGgtaagt ttaaacatat atatactaac taaccctgat tatttaaatt
     1261 ttcagCCGAC CCTTGTGACT ACATTCTGCT ACGGAGTTCA ATGCTTTTCC CGCTACCCAG
     1321 ACCACATGAA GCGTCACGAT TTCTTCAAAT CAGCCATGCC AGAAGGATAC GTCCAGGAGC
     1381 GAACAATTTT CTTCAAGGAC GATGGAAACT ACAAGACTCg taagtttaaa cagttcggta
     1441 ctaactaacc atacatattt aaattttcag GTGCTGAAGT CAAGTTTGAA GGAGATACTC
     1501 TCGTGAACCG CATTGAGCTC AAGGGAATCG ACTTCAAAGA AGATGGAAAC ATTCTTGGAC
     1561 ACAAGCTCGA ATACAACTAT AACTCGCACA ACGTGTATAT CATGGCCGAC AAGCAAAAAA
     1621 ATGGAATCAA GGTCgtaagt ttaaacatga ttttactaac taactaatct gatttaaatt
     1681 ttcagAACTT CAAGATTCGC CACAACATCG AAGACGGGTC GGTTCAACTC GCTGATCACT
     1741 ACCAGCAGAA CACACCAATT GGAGATGGAC CAGTCCTCCT CCCTGATAAT CACTACCTTT
     1801 CCACTCAATC CGCTCTTAGC AAGGATCCAA ATGAGAAAAG AGATCACATG GTTCTTCTCG
     1861 AGTTTGTCAC CGCCGCCGGA ATCACCCACG GAATGGACGA GTTGTACAAA GAGAATCTGT
     1921 ACTTTCAATC CGGAAAGgta agtttaaaAT AACTTCGTAT AGCATACATT ATACGAAGTT
     1981 ATtttcagGG AGAGCAAAAG CTTATCTCCG AGGAGGACCT CTAAGGATGA TCGACGCCaA
     2041 CGTCGTTGAA TTTTCAAATT TTAAATACTG AATATTTGTT TTTTTTCCTA TTATTTATTT
     2101 ATTCTCTTTG TGTTTTTTTT CTTGCTTTCT AAAAAATTAA TTCAATCCAA ATCTAAacat
     2161 ttttttttct ctttccgtct cccaattcgt attccgctcc tctcatctga acacaatgtg
     2221 caagtttatt tatcttctcg ctttcatttc attaggacgt ggggggaatt ggtggaaggg
     2281 ggaaacacac aaaaggatga tggaaatgaa ataaggacac acaatatgca acaacattca
     2341 attcagaaat atggaggaag gtttaaaaga aaacataaaa atatatagag gaggaaggaa
     2401 actagctgcc ctcgattgtc tatcttccat tctcaccaag tttcaacaac tttattcagt
     2461 cattcttctc tggttcattt cccaaccaat cttcccgaat aaagcccatc tgtcctttcg
     2521 gccacattcc tcacctacat ccgtactcct tatctcccgt tcttatccag tcttttatcg
     2581 tctctgcgtc tcgcacactg tctcgtcggt aatgtaaaaa aaaaacaaaa aaaacttgaa
     2641 aacttgcgga aagatttcct aataaaagga gacgaatccg gtaggaaatc gacaagtctc
     2701 tgcttctcga agactaggtg agttacattt ctttggccga attattttga aatcctaaat
     2761 ttgataattt cagagATGTC TGTAAAACTT GCGTGTTATG TGACGGCTTC GGTCACTGTC
     2821 GCCACTCTTA TGGTATGCTT CATGACCATG TCAACCATCT ACTCGGAGGT TGATGGATTC
     2881 AGAGAGAAGC TTGATACCGA GATGAATGTG TTCAGAgtga gttaaattat attttgaatt
     2941 ttaaataatt tttaattttc tagCAATCTA CCAATGGATT GTGGAAGGAC ATAGTTGTCA
     3001 TCGGAAGATC TAGCAAGCGT GTCCGTTGTC AATATGAAGA GACCAACGCT ACCCCAACTC
     3061 CACATGCTGA TGGATCCCCA TCTGCTCCAC CAGGTCAACC ACCAGCAGTT CCACCAGTCT
     3121 TCAACCAGCC AAAGACTCCA AATGGAGCCA ATGGAAATGG ACCAACCTGC AACTGCAATG
     3181 CTGATAACAA GTGCCCAGCT GGACCATCCG GACCAAAGGG AGTTCCAGGA GTTCCAGGAC
     3241 TCGACGGAGT TCCAGGACTT GACGGTGTTC CAGGAGTTGG AGCTGATGAT ATCGCTCCAC
     3301 AACGCGAGTC TGTCGGATGC TTCACTTGCC CACAAGGACC AGTTGGACCA CCAGGAGCTC
     3361 TTGGAAGACC AGGACCACGT GGACTTCCAG GACCAAGAGG ACAAAATGGA AACCCAGGAA
     3421 GAGATGGACA ACCAGGACAT CCAGGAGAGC AAGGATCATC CGGTCAAATC GGAAAGATCG
     3481 GAGAGCCAGG ACCACCAGGA GAGAAGGGAC GCGACGCCGA GCATCCAATC GGAAGACCAG
     3541 GACCAAAGGG ACCAAGAGGA GATCAAGGAC CAACAGGACC AGCTGGACAG AACGGTCTTC
     3601 ACGGACCACC AGGAGAGCCA GGAACCGTTG GACCAGAAGG ACCATCTGGA AAGCAAGGAC
     3661 GTCAAGGACC AGACGGAACC CAGGGAGAGA CTGGACCAGA CGGAAGACCA GGAAAGGATG
     3721 CCGAGTACTG CCAGTGCCCA GACAAGTCTC CACCATCAGA GGCTGTCAAC GCCAACCGTG
     3781 GATACAGAAA TATCTAAatt gttggtgttt tctaataaaa tatttgagat tcagtgagac
     3841 ttttttcttg gcggcacaat aaagtttcct ctttttgttg ggtcactcaa cgaacacaat
     3901 acaaatattt gagcgctcgc atttttctca acctttcggg tttaaagcac ttcaaaatgg
     3961 aattgaagag agattgtgaa agtacatcag acagaatcac attttgatag cattcacttc
     4021 actcagatgc aaacttttca gtgactataa aaagtcataa aataataaca ttaaaaaagc
     4081 aaataaatta gcgagagaat tttttgacaa aaagaaagaa gagtgataga gaagaaggga
     4141 atgcttgaaa ggattttgca tttatcttaA TCACCGTCTT CGAGAAGACG AACCATGGCG
     4201 CCAGTTTCAG AATCCAAGTT TCTAATATAG TTCATCACAA TGTTGACGTT GGTCCAACCT
     4261 CCGGCTTGCA TAATCTCTGG GATGGAAACT CCAGCGCGAG CCATGTCACG GGCAGCTCCG
     4321 ACACGGGCGG AATGTCCAGA CCAAGCCAAG TAACGTTGTC CGGAATCATC CTTGGCTCCG
     4381 TAGATGAGAC GGTGGGTAGC TTCGAAGATT Cctgaaaatt tattttcagc ttattcttag
     4441 ctaatatgtt gtttaaactt acCTTCAAGA GCTCGAGTCG AGAGTTGTGA TGTTGCACTC
     4501 GGGGCAGCAA CTCCGTTCTT GCGAACACGA CAGAACAGGT AGTTGTTTGG ATCATCGGCG
     4561 ACTCCGGAAA CCGAAATCCA GCGCTCAACG AGCTTGGTAA CTCCAAGGGA GAGCGCTTTT
     4621 TCGACTCCGG CAGTCGAGAC CAGCGTTTTT GTACGTCCGA TGTGGATGAG CATTCTTCCT
     4681 CCGTCTGTGC GACTAATATC CTTGACGCGA ATGCGGGCAA TTTCGGCGAT ACGGAGAAGT
     4741 GTGTTGTAAG CAATTCCGAG GAAGGCGAGG TTTCGGATGT CCTGGCAACG ATCGGAATTT
     4801 TCCATAAGAG AACGGACCTG ATCGAAGTCG GTTctgaaaa tttcgtcaag tcttaggatt
     4861 gaatacatta atatttaaac ttacCTTTCG AAGGCGAGGG CTTGCTTGGC TCTCTCTCCA
     4921 GCATCAACAT TTTCCTTGCG GATACGTCTC ATTACGAGAG ATACGGCATT AGAATCTGAT
     4981 GGGCGTGGAA GTCCAGATCG GCGATGGAGC ATATTGAGCT GTCCGAGATG TTGTTGGATC
     5041 GTCTTCACCG CCAATCCACG AGCTTGGAGG TAGAGCAAGT AGTCACGAAC GTCTTCTGGC
     5101 TCGGCTGGGA ACCACTTGCG GTTGTTAAGC TTGCACCAGG CAGCCCAAGA ACGGCACACT
     5161 GAGAGAAGCA TCTTCCAGGT GTGCTCGGAG AAGGCTTGTC TGTCACGGAA CATATCCATG
     5221 AGGTTCTTGC GAACTTCATC ACTTGTCGCG TCAACTGGAA GTGCTGGAAG ATTTTGATGA
     5281 ACAGTGAGAA GATTTGACAT attttcgaag ttttttagat gcactagaac aaagcgtgtt
     5341 ggcttcctct gagcccgctt tccttatata cccgcattct gcagccttac agaatgttct
     5401 agaaggtcct agatgcattc gtttgaaaat actcccggtg ggtgcaaaga gacgcagacg
     5461 gaaaatgtat ctgggtctct ttattgtgta cactactttt ccatgtaccg aatgtgagtc
     5521 gccctccttt tgcaacaagc agctcgaatg ttctagaaaa aggtggaaaa tagtataaat
     5581 accgttgaaa ataaataccg aacaacattt gctctaattg tgaaattaga aatcttcaaa
     5641 ctataatcat gtcactttac cactatttcc gtccagctca acgttccgtt tttggtgatc
     5701 ATTTTTGCTT TCGTCGTAAA TCTACACACG CGTCTCTTCC GTGCGAGAGT CCAAGCCAGC
     5761 AGCCAAATTC GTTGACTGAG TATTCAACGT TTATACGTTG TCGGCAACGA GAAATAGGAA
     5821 AATGCATCGG GAAATGTTCT TTTTTCGATT TTTTCCAAGG TTTTGACAAA TTTTACCACG
     5881 AATTTTGCTA TGTTTTCAAT TAAAAAATAT GTTATTCAAC TGTTTCTATG AGGAAAATAA
     5941 GGCTTTGCAT GTAATTTTCT TATTCAGCAT AATTTTTAAT TAATTTGAAT TTTCTGTCCT
     6001 AACGTTTATT TTGTTTTCTT GGTTATGACT GATCTGAAAT TAATTTTTGA ATTTTAAGGT
     6061 AATATGTCAG GCGGTGCCGC AAGTTTGTAC AAAAAAGCAG GCTCCATGAA AAAGCCTGAA
     6121 CTCACCGCGA CGTCTGTCGA GAAGTTTCTG ATCGAAAAGT TCGACAGCGT CTCCGACCTG
     6181 ATGCAGCTCT CGGAGGGCGA AGAATCTCGT GCTTTCAGCT TCGATGTAGG AGGGCGTGGA
     6241 TATGTCCTGC GGGTAAATAG CTGCGCCGAT GGTTTCTACA AAGATCGTTA TGTTTATCGG
     6301 CACTTTGCAT CGGCCGCGCT CCCGATTCCG GAAGTGCTTG ACATTGGGGA ATTCAGCGAG
     6361 AGCCTGACCT ATTGCATCTC CCGCCGTGCA CAGGGTGTCA CGTTGCAAGA CCTGCCTGAA
     6421 ACCGAACTGC CCGCTGTTCT GCAGCCGGTC GCGGAGGCCA TGGATGCGAT CGCTGCGGCC
     6481 GATCTTAGCC AGACGAGCGG GTTCGGCCCA TTCGGACCGC AAGGAATCGG TCAATACACT
     6541 ACATGGCGTG ATTTCATATG CGCGATTGCT GATCCCCATG TGTATCACTG GCAAACTGTG
     6601 ATGGACGACA CCGTCAGTGC GTCCGTCGCG CAGGCTCTCG ATGAGCTGAT GCTTTGGGCC
     6661 GAGGACTGCC CCGAAGTCCG GCACCTCGTG CACGCGGATT TCGGCTCCAA CAATGTCCTG
     6721 ACGGACAATG GCCGCATAAC AGCGGTCATT GACTGGAGCG AGGCGATGTT CGGGGATTCC
     6781 CAATACGAGG TCGCCAACAT CTTCTTCTGG AGGCCGTGGT TGGCTTGTAT GGAGCAGCAG
     6841 ACGCGCTACT TCGAGCGGAG GCATCCGGAG CTTGCAGGAT CGCCGCGGCT CCGGGCGTAT
     6901 ATGCTCCGCA TTGGTCTTGA CCAACTCTAT CAGAGCTTGG TTGACGGCAA TTTCGATGAT
     6961 GCAGCTTGGG CGCAGGGTCG ATGCGACGCA ATCGTCCGAT CCGGAGCCGG GACTGTCGGG
     7021 CGTACACAAA TCGCCCGCAG AAGCGCGGCC GTCTGGACCG ATGGCTGTGT AGAAGTACTC
     7081 GCCGATAGTG GAAACCGACG CCCCAGCACT CGTCCGAGGG CAAAGGAATA GACCCAGCTT
     7141 TCTTGTACAA AGTGGGTCCA ATTACTCTTC AACATCCCTA CATGCTCTTT CTCCCTGTGC
     7201 TCCCACCCCC TATTTTTGTT ATTATCAAAA AACTTCTCTT AATTTCTTTG TTTTTTAGCT
     7261 TCTTTTAAGT CACCTCTAAC AATGAAATTG TGTAGATTCA AAAATAGAAT TAATTCGTAA
     7321 TAAAAAGTCG AAAAAAATTG TGCTCCCTCC CCCCATTAAT AATAATTCTA TCCCAAAATC
     7381 TACACAATGT TCTGTGTACA CTTCTTATGT TTTTTACTTC TGATAAATTT TTTTGAAACA
     7441 TCATAGAAAA AACCGCACAC AAAATACCTT ATCATATGTT ACGTTTCAGT TTATGACCGC
     7501 AATTTTTAAT AACTTCGTAT AGCATACATT ATACGAAGTT ATtttcagGG AGCCGGATCT
     7561 GATTATAAAG ACGATGACGA TAAGCGTGAC TACAAGGACG ACGACGACAA GCGTGATTAC
     7621 AAGGATGACG ATGACAAGAG ActagtTCGA CCTGCAGACT GGCTGTGTAT AAGGGAGCCT
     7681 GACATTTATA TTCCCCAGAA CATCAGGTTA ATGGCGTTTT TGATGTCATT TTCGCGGTGG
     7741 CTGAGATCAG CCACTTCTTC CCCGATAACG GAGACCGGCA CACTGGCCAT ATCGGTGGTC
     7801 ATCATGCGCC AGCTTTCATC CCCGATATGC ACCACCGGGT AAAGTTCACG GGGGACTTTA
     7861 TCTGACAGCA GACGTGCACT GGCCAGGGGG ATCACCATCC GTCGCCCGGG CGTGTCAATA
     7921 ATATCACTCT GTACATCCAC AAACAGACGA TAACGGCTCT CTCTTTTATA GGTGTAAACC
     7981 TTAAACTGCA TTTCACCAGC CCCTGTTCTC GTCGGCAAAA GAGCCGTTCA TTTCAATAAA
     8041 CCGGGCGACC TCAGCCATCC CTTCCTGATT TTCCGCTTTC CAGCGTTCGG CACGCAGACG
     8101 ACGGGCTTCA TTCTGCATGG TTGTGCTTAC CGAACCGGAG ATATTGACAT CATATATGCC
     8161 TTGAGCAACT GATAGCTGTC GCTGTCAACT GTCACTGTAA TACGCTGCTT CATAGCATAC
     8221 CTCTTTTTGA CATACTTCGG GTtcctagga aatcgataac atggtcatag ctgtttcctg
     8281 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     8341 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     8401 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     8461 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     8521 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     8581 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     8641 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     8701 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     8761 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     8821 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     8881 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     8941 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     9001 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     9061 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     9121 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     9181 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     9241 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     9301 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     9361 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     9421 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     9481 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     9541 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     9601 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     9661 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     9721 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     9781 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     9841 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     9901 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     9961 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    10021 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
    10081 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    10141 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    10201 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    10261 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    10321 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    10381 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
    10441 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtc 
//
