LOCUS       Pmyo2_GFP_donor         3433 bp ds-DNA     circular     04-MAY-2016
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     Insert from SapTrap FP 13 to 916
COMMENT     Backbone from 084_pDONR221 2897 to 651
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_recomb     652..668
                     /label=attB1
                     /ApEinfo_fwdcolor=#cccccc
                     /ApEinfo_revcolor=#cccccc
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     terminator      complement(268..295)
                     /label=rrnB_T2_terminator
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     1558..1567
                     /label=attB2r
                     /ApEinfo_fwdcolor=#cccccc
                     /ApEinfo_revcolor=#cccccc
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     terminator      complement(427..470)
                     /label=rrnB_T1_terminator
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    669..675
                     /label=SapI
                     /ApEinfo_fwdcolor=#ea17ff
                     /ApEinfo_revcolor=#ea17ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1551..1557
                     /label=SapI(1)
                     /ApEinfo_label=SapI
                     /ApEinfo_fwdcolor=#ea17ff
                     /ApEinfo_revcolor=#ea17ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    677..679
                     /label=ATG Overlap
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=cyan
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    570..651
                     /label=attP
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(1568..1656)
                     /label=attP(1)
                     /ApEinfo_label=attP
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             join(680..853,905..1058,1110..1273,1325..1546)
                     /label=GFP-GLO
                     /ApEinfo_fwdcolor=#33cc66
                     /ApEinfo_revcolor=#006666
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        complement(1675..1693)
                     /label=T7_promoter
                     /ApEinfo_fwdcolor=#33cc00
                     /ApEinfo_revcolor=#33cc00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(1694..1712)
                     /label=M13_reverse_primer
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      2767..3386
                     /label=pBR322_origin
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(1694..1714)
                     /label=M13-rev
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(1674..1695)
                     /label=T7
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             1827..2633
                     /label=KanR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     536..553
                     /label=M13-fwd
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1547..1549
                     /label=AAG overlap
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
      601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
      661 AGCAGGCTGC TCTTCgATGC GTTCTTCCAA AGGAGAAGAA CTCTTTACCG GAGTCGTCCC
      721 AATTCTCGTC GAGCTCGACG GAGACGTAAA CGGACACAAA TTCTCGGTTT CCGGAGAAGG
      781 AGAAGGAGAT GCTACTTATG GAAAACTCAC CCTTAAATTC ATTTGCACCA CCGGAAAGCT
      841 CCCAGTGCCG TGGgtaagtt taaacatata tatactaact aaccctgatt atttaaattt
      901 tcagCCGACC CTTGTGACTA CATTCTGCTA CGGAGTTCAA TGCTTTTCCC GCTACCCAGA
      961 CCACATGAAG CGTCACGATT TCTTCAAATC AGCCATGCCA GAAGGATACG TCCAGGAGCG
     1021 AACAATTTTC TTCAAGGACG ATGGAAACTA CAAGACTCgt aagtttaaac agttcggtac
     1081 taactaacca tacatattta aattttcagG TGCTGAAGTC AAGTTTGAAG GAGATACTCT
     1141 CGTGAACCGC ATTGAGCTCA AGGGAATCGA CTTCAAAGAA GATGGAAACA TTCTTGGACA
     1201 CAAGCTCGAA TACAACTATA ACTCGCACAA CGTGTATATC ATGGCCGACA AGCAAAAAAA
     1261 TGGAATCAAG GTCgtaagtt taaacatgat tttactaact aactaatctg atttaaattt
     1321 tcagAACTTC AAGATTCGCC ACAACATCGA AGACGGGTCG GTTCAACTCG CTGATCACTA
     1381 CCAGCAGAAC ACACCAATTG GAGATGGACC AGTCCTCCTC CCTGATAATC ACTACCTTTC
     1441 CACTCAATCC GCTCTTAGCA AGGATCCAAA TGAGAAAAGA GATCACATGG TTCTTCTCGA
     1501 GTTTGTCACC GCCGCCGGAA TCACCCACGG AATGGACGAG TTGTACAAGc GAAGAGCACC
     1561 CAGCTTTCTT GTACAAAGTT GGCATTATAA GAAAGCATTG CTTATCAATT TGTTGCAACG
     1621 AACAGGTCAC TATCAGTCAA AATAAAATCA TTATTTGCCA TCCAGCTGAT ATCCCCTATA
     1681 GTGAGTCGTA TTACATGGTC ATAGCTGTTT CCTGGCAGCT CTGGCCCGTG TCTCAAAATC
     1741 TCTGATGTTA CATTGCACAA GATAAAATAA TATCATCATG AACAATAAAA CTGTCTGCTT
     1801 ACATAAACAG TAATACAAGG GGTGTTATGA GCCATATTCA ACGGGAAACG TCGAGGCCGC
     1861 GATTAAATTC CAACATGGAT GCTGATTTAT ATGGGTATAA ATGGGCTCGC GATAATGTCG
     1921 GGCAATCAGG TGCGACAATC TATCGCTTGT ATGGGAAGCC CGATGCGCCA GAGTTGTTTC
     1981 TGAAACATGG CAAAGGTAGC GTTGCCAATG ATGTTACAGA TGAGATGGTC AGACTAAACT
     2041 GGCTGACGGA ATTTATGCCT CTTCCGACCA TCAAGCATTT TATCCGTACT CCTGATGATG
     2101 CATGGTTACT CACCACTGCG ATCCCCGGAA AAACAGCATT CCAGGTATTA GAAGAATATC
     2161 CTGATTCAGG TGAAAATATT GTTGATGCGC TGGCAGTGTT CCTGCGCCGG TTGCATTCGA
     2221 TTCCTGTTTG TAATTGTCCT TTTAACAGCG ATCGCGTATT TCGTCTCGCT CAGGCGCAAT
     2281 CACGAATGAA TAACGGTTTG GTTGATGCGA GTGATTTTGA TGACGAGCGT AATGGCTGGC
     2341 CTGTTGAACA AGTCTGGAAA GAAATGCATA AACTTTTGCC ATTCTCACCG GATTCAGTCG
     2401 TCACTCATGG TGATTTCTCA CTTGATAACC TTATTTTTGA CGAGGGGAAA TTAATAGGTT
     2461 GTATTGATGT TGGACGAGTC GGAATCGCAG ACCGATACCA GGATCTTGCC ATCCTATGGA
     2521 ACTGCCTCGG TGAGTTTTCT CCTTCATTAC AGAAACGGCT TTTTCAAAAA TATGGTATTG
     2581 ATAATCCTGA TATGAATAAA TTGCAGTTTC ATTTGATGCT CGATGAGTTT TTCTAATCAG
     2641 AATTGGTTAA TTGGTTGTAA CACTGGCAGA GCATTACGCT GACTTGACGG GACGGCGCAA
     2701 GCTCATGACC AAAATCCCTT AACGTGAGTT ACGCGTCGTT CCACTGAGCG TCAGACCCCG
     2761 TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC
     2821 AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC
     2881 TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTT CTTCTAGTGT
     2941 AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC
     3001 TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT
     3061 CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC
     3121 AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG
     3181 AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG
     3241 GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG
     3301 TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA
     3361 GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT
     3421 TTGCTCACAT GTT    
//
