LOCUS       SapTrap_mNG_dono        3607 bp ds-DNA     circular     28-MAR-2016
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     Insert from SapTrap FP 13 to 916
COMMENT     Backbone from 084_pDONR221 2897 to 651
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_recomb     652..668
                     /label=attB1
                     /ApEinfo_fwdcolor=#cccccc
                     /ApEinfo_revcolor=#cccccc
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     terminator      complement(268..295)
                     /label=rrnB_T2_terminator
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     1732..1741
                     /label=attB2r
                     /ApEinfo_fwdcolor=#cccccc
                     /ApEinfo_revcolor=#cccccc
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     terminator      complement(427..470)
                     /label=rrnB_T1_terminator
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    669..675
                     /label=SapI
                     /ApEinfo_fwdcolor=#ea17ff
                     /ApEinfo_revcolor=#ea17ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1725..1731
                     /label=SapI(1)
                     /ApEinfo_label=SapI
                     /ApEinfo_fwdcolor=#ea17ff
                     /ApEinfo_revcolor=#ea17ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    570..651
                     /label=attP
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1721..1723
                     /label=AAG overlap
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(1742..1830)
                     /label=attP(1)
                     /ApEinfo_label=attP
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        complement(1849..1867)
                     /label=T7_promoter
                     /ApEinfo_fwdcolor=#33cc00
                     /ApEinfo_revcolor=#33cc00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(1868..1886)
                     /label=M13_reverse_primer
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      2941..3560
                     /label=pBR322_origin
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(1868..1888)
                     /label=M13-rev
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(1848..1869)
                     /label=T7
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             2001..2807
                     /label=KanR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     536..553
                     /label=M13-fwd
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     1241..1259
                     /label=HaloTag-GLO Int Seq P987
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=#00cc99
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             join(677..869,921..1168,1220..1418,1470..1720)
                     /label=HaloTag
                     /ApEinfo_fwdcolor=#cc33cc
                     /ApEinfo_revcolor=#006666
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
      601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
      661 AGCAGGCTGC TCTTCgATGG CCGAAATCGG AACTGGATTC CCATTCGACC CCCATTATGT
      721 TGAGGTTCTT GGAGAACGCA TGCATTATGT TGATGTAGGA CCACGAGATG GAACTCCAGT
      781 TCTCTTCCTT CACGGAAATC CGACTTCTTC ATATGTCTGG AGAAACATCA TTCCACACGT
      841 TGCTCCAACA CACAGATGTA TTGCTCCAGg taagtttagg catatgtgca catctgttca
      901 tggacaccat gatttttcag ACCTCATCGG AATGGGAAAA TCGGATAAGC CAGATCTTGG
      961 ATACTTCTTC GATGATCATG TTCGATTCAT GGATGCCTTC ATCGAGGCTC TTGGACTCGA
     1021 AGAAGTCGTC CTCGTCATAC ATGATTGGGG ATCTGCTCTT GGATTCCATT GGGCCAAGAG
     1081 AAACCCGGAG CGTGTCAAGG GAATTGCTTT CATGGAGTTT ATTCGCCCAA TTCCAACTTG
     1141 GGATGAGTGG CCTGAATTCG CCCGCGAGgt aagtttgcta aagaataaac ctgatgtatt
     1201 tcatggtccg tattttcagA CCTTCCAAGC ATTCCGGACC ACCGATGTCG GAAGAAAGCT
     1261 TATCATTGAT CAAAACGTTT TCATTGAAGG AACTCTTCCG ATGGGAGTTG TCCGTCCACT
     1321 TACCGAAGTC GAAATGGACC ACTACAGAGA GCCGTTCCTC AACCCTGTCG ACAGAGAACC
     1381 ACTCTGGAGG TTTCCAAATG AGTTGCCGAT CGCTGGAGgt aagtttcttg ctattataat
     1441 aacacgatca aataatactt tcttttcagA GCCGGCTAAC ATTGTTGCTC TTGTTGAGGA
     1501 GTACATGGAC TGGCTGCACC AAAGTCCAGT TCCAAAACTC CTTTTCTGGG GAACTCCAGG
     1561 AGTCCTTATT CCACCTGCTG AAGCCGCCCG TCTTGCCAAG TCCCTCCCAA ACTGCAAGGC
     1621 CGTCGATATC GGACCGGGAC TCAACTTGCT CCAAGAAGAC AACCCAGACC TCATCGGATC
     1681 CGAAATTGCC CGCTGGCTCT CCACCCTTGA GATTTCTGGA AAGcGAAGAG CACCCAGCTT
     1741 TCTTGTACAA AGTTGGCATT ATAAGAAAGC ATTGCTTATC AATTTGTTGC AACGAACAGG
     1801 TCACTATCAG TCAAAATAAA ATCATTATTT GCCATCCAGC TGATATCCCC TATAGTGAGT
     1861 CGTATTACAT GGTCATAGCT GTTTCCTGGC AGCTCTGGCC CGTGTCTCAA AATCTCTGAT
     1921 GTTACATTGC ACAAGATAAA ATAATATCAT CATGAACAAT AAAACTGTCT GCTTACATAA
     1981 ACAGTAATAC AAGGGGTGTT ATGAGCCATA TTCAACGGGA AACGTCGAGG CCGCGATTAA
     2041 ATTCCAACAT GGATGCTGAT TTATATGGGT ATAAATGGGC TCGCGATAAT GTCGGGCAAT
     2101 CAGGTGCGAC AATCTATCGC TTGTATGGGA AGCCCGATGC GCCAGAGTTG TTTCTGAAAC
     2161 ATGGCAAAGG TAGCGTTGCC AATGATGTTA CAGATGAGAT GGTCAGACTA AACTGGCTGA
     2221 CGGAATTTAT GCCTCTTCCG ACCATCAAGC ATTTTATCCG TACTCCTGAT GATGCATGGT
     2281 TACTCACCAC TGCGATCCCC GGAAAAACAG CATTCCAGGT ATTAGAAGAA TATCCTGATT
     2341 CAGGTGAAAA TATTGTTGAT GCGCTGGCAG TGTTCCTGCG CCGGTTGCAT TCGATTCCTG
     2401 TTTGTAATTG TCCTTTTAAC AGCGATCGCG TATTTCGTCT CGCTCAGGCG CAATCACGAA
     2461 TGAATAACGG TTTGGTTGAT GCGAGTGATT TTGATGACGA GCGTAATGGC TGGCCTGTTG
     2521 AACAAGTCTG GAAAGAAATG CATAAACTTT TGCCATTCTC ACCGGATTCA GTCGTCACTC
     2581 ATGGTGATTT CTCACTTGAT AACCTTATTT TTGACGAGGG GAAATTAATA GGTTGTATTG
     2641 ATGTTGGACG AGTCGGAATC GCAGACCGAT ACCAGGATCT TGCCATCCTA TGGAACTGCC
     2701 TCGGTGAGTT TTCTCCTTCA TTACAGAAAC GGCTTTTTCA AAAATATGGT ATTGATAATC
     2761 CTGATATGAA TAAATTGCAG TTTCATTTGA TGCTCGATGA GTTTTTCTAA TCAGAATTGG
     2821 TTAATTGGTT GTAACACTGG CAGAGCATTA CGCTGACTTG ACGGGACGGC GCAAGCTCAT
     2881 GACCAAAATC CCTTAACGTG AGTTACGCGT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA
     2941 AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA
     3001 AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC
     3061 CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT
     3121 AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC
     3181 TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC
     3241 GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA
     3301 GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG
     3361 CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG
     3421 GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT
     3481 TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT
     3541 GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC
     3601 ACATGTT     
//
