LOCUS       pRSET-TEV-12His        3281 bp    DNA     circular     27-NOV-2019
DEFINITION  T7 driven prokaryotic expression vector (Invitrogen).
SOURCE      
  ORGANISM  
COMMENT     Vector idea from Debra T. Hansen; vector design and cloning by Felicia M. Craciunescu;
            for CASD projects.
            
            The "stuffer" DNA sequence encodes a 132 aa fragment from the 485 aa Bacillus
            anthracis conjugation protein, TraG-TraD family, protein access. no. GAF01267.1
            (however, the codon usage is different from the corresponding DNA access. no.
            BAZ_5751). This BseRI-flanked segment is removed upon cloning into the BseRI sites.
            
            
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|627388011|
COMMENT     VNTDBDATE|-13097749|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pRSET-TEV-12His|
COMMENT     VNTAUTHORNAME|Debra T Hansen|
COMMENT     VNTAUTHORTEL|000-000-0000|
COMMENT     VNTAUTHOREML|debra.hansen.1@asu.edu|
COMMENT     VNTOAUTHORNAME|Alex Borovkov|
COMMENT     VNTOAUTHOREML|ayboro@asu.edu|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT         25 26 55 91 27 28 29 30 31 92 32 34 35 33 53 58 93 56 94 37 105 57 38 
COMMENT         36 39 40 41 42 43 95 44 59 99 96 97 45 100 46) (CWordArray) 
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COMMENT                          "T7 driven prokaryotic expression vector (Invitrogen)." 
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COMMENT                          "Currently local object. Original author: Alex Borovkov" 
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FEATURES             Location/Qualifiers
     promoter        20..39
                     /vntifkey="30"
                     /label=T7\promoter
     rep_origin      840..1295
                     /vntifkey="33"
                     /label=f1\ori
     CDS             1426..2286
                     /vntifkey="4"
                     /label=AMP-R
     promoter        1327..1425
                     /vntifkey="30"
                     /label=bla\pr
     rep_origin      2431..3104
                     /vntifkey="33"
                     /label=pUC\ori
     RBS             85..92
                     /vntifkey="32"
                     /label=RBS
     terminator      640..769
                     /vntifkey="43"
                     /label=T7\term
     misc_feature    99..99
                     /vntifkey="21"
                     /label=upstream\In-Fusion\site
     misc_feature    553..588
                     /vntifkey="21"
                     /label=His12
     misc_feature    589..594
                     /vntifkey="21"
                     /label=stop\codons
     misc_feature    532..552
                     /vntifkey="21"
                     /label=TEV\protease\cleavage
     misc_feature    532..532
                     /vntifkey="21"
                     /label=downstream\In-Fusion\site
     misc_feature    100..531
                     /vntifkey="21"
                     /label=REMOVED\upon\In-Fusion\cloning\into\BseRI\sites
     primer          20..39
                     /vntifkey="27"
                     /label=PRIMER\T7
     primer          complement(682..700)
                     /vntifkey="27"
                     /label=PRIMER\T7-Ter
     misc_feature    110..115
                     /vntifkey="21"
                     /label=BseRI\recognition\site\1
     misc_feature    516..521
                     /vntifkey="21"
                     /label=BseRI\recognition\site\2
BASE COUNT      836 a       833 c       806 g       806 t 
ORIGIN
        1 gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct 
       61 agaaataatt ttgtttaact ttaagaagga gatatacata gatctgtcgc tcctcatgta 
      121 gacaccgaca ctgtgaacac cgccatcctg ggcgtttcaa ggtccggcaa aggcgagaca 
      181 atcgtagtgc caatgatcga caatctgagc cgcgcaaaga accagtctag catggtcgtg 
      241 aacgacccca agggcgagct ctactctgcc agcaaggaaa cacttgagaa acgcggatac 
      301 gacgtgcagg tgctgaacat cctggatccc ctgcagggca tgagttacaa cccactccag 
      361 ctcgtgatcg acgcctgggt gaacggcgac gaccaggagg ctgcaaagag ggcaaacaca 
      421 ctgactttct cactgtacaa caacccaaat gctggcgaca acgctttctt taacaccagc 
      481 gcccagaatg ctatcaacgg aatcatcctc gctatgagga ggcagaagct tgaaaatctt 
      541 tattttcaag gtcaccatca tcaccaccat caccatcatc accaccatta ataaaagggc 
      601 gaattccagc acactggcgg ccgttactag tggatccggc tgctaacaaa gcccgaaagg 
      661 aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta 
      721 aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatct ggcgtaatag 
      781 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga 
      841 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc 
      901 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac 
      961 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag 
     1021 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc 
     1081 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg 
     1141 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata 
     1201 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa 
     1261 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg 
     1321 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga 
     1381 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat 
     1441 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca 
     1501 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc 
     1561 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca 
     1621 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg 
     1681 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca 
     1741 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata 
     1801 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag 
     1861 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg 
     1921 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca 
     1981 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta 
     2041 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct 
     2101 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca 
     2161 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag 
     2221 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat 
     2281 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt 
     2341 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa 
     2401 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 
     2461 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 
     2521 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 
     2581 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag 
     2641 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 
     2701 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 
     2761 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 
     2821 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 
     2881 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 
     2941 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 
     3001 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 
     3061 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 
     3121 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc 
     3181 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc 
     3241 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca g 
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