LOCUS       pBBA57-TEV-His12        4091 bp    DNA     circular      3-DEC-2019
DEFINITION  T7 driven prokaryotic expression vector (Invitrogen).
SOURCE      
  ORGANISM  
COMMENT     Vector design and cloning by Debra T. Hansen and Felicia M. Craciunescu.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|627388011|
COMMENT     VNTDBDATE|-13106328|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pBBA57-TEV-His12|
COMMENT     VNTAUTHORNAME|Debra T Hansen|
COMMENT     VNTAUTHORTEL|000-000-0000|
COMMENT     VNTAUTHOREML|debra.hansen.1@asu.edu|
COMMENT     VNTOAUTHORNAME|Alex Borovkov|
COMMENT     VNTOAUTHOREML|ayboro@asu.edu|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT         "AcuI" "AfeI" "AflII" "AflIII" "AgeI" "AhdI" "AleI" "AloI" "AluI" 
COMMENT         "AlwI" "AlwNI" "ApaBI" "ApaI" "ApaLI" "ApoI" "AscI" "AseI" "AsiSI" 
COMMENT         "AvaI" "AvaII" "AvrII" "BaeI" "BamHI" "BanI" "BanII" "BbeI" "Bbr7I" 
COMMENT         "BbsI" "BbvCI" "BbvI" "BccI" "BceAI" "BcefI" "BcgI" "BciVI" "BclI" 
COMMENT         "BfaI" "BfrBI" "BglI" "BglII" "BlpI" "Bme1580I" "BmgBI" "BmrI" "BmtI" 
COMMENT         "BplI" "BpmI" "Bpu10I" "BpuEI" "BsaAI" "BsaBI" "BsaHI" "BsaI" "BsaJI" 
COMMENT         "BsaWI" "BsaXI" "BscAI" "BseMII" "BseRI" "BseYI" "BsgI" "BsiEI" 
COMMENT         "BsiHKAI" "BsiWI" "BslI" "BsmAI" "BsmBI" "BsmFI" "BsmI" "Bsp1286I" 
COMMENT         "Bsp24I" "BspCNI" "BspEI" "BspHI" "BspMI" "BsrBI" "BsrDI" "BsrFI" 
COMMENT         "BsrGI" "BsrI" "BssHII" "BssSI" "BstAPI" "BstBI" "BstEII" "BstF5I" 
COMMENT         "BstKTI" "BstNI" "BstUI" "BstXI" "BstYI" "BstZ17I" "Bsu36I" "BtgI" 
COMMENT         "BthCI" "BtsI" "Cac8I" "CdiI" "ChaI" "CjeI" "CjePI" "ClaI" "Csp6I" 
COMMENT         "CviAII" "CviJI" "DdeI" "DpnI" "DraI" "DraIII" "DrdI" "EaeI" "EagI" 
COMMENT         "EarI" "EciI" "Eco57MI" "EcoHI" "EcoICRI" "EcoNI" "EcoO109I" "EcoRI" 
COMMENT         "EcoRV" "EsaBC3I" "FalI" "FatI" "FauI" "FmuI" "Fnu4HI" "FokI" "FseI" 
COMMENT         "FspAI" "FspI" "GdiII" "HaeI" "HaeII" "HaeIII" "HaeIV" "HgaI" "HhaI" 
COMMENT         "Hin4I" "HincII" "HindIII" "HinfI" "HinP1I" "HpaI" "HpaII" "HphI" 
COMMENT         "Hpy188I" "Hpy188III" "Hpy8I" "Hpy99I" "HpyAV" "HpyCH4I" "HpyCH4III" 
COMMENT         "HpyCH4IV" "HpyCH4V" "HpyF10VI" "KasI" "KpnI" "LpnI" "MaeIII" "MboI" 
COMMENT         "MboII" "MfeI" "MluI" "MlyI" "MmeI" "MnlI" "MscI" "MseI" "MslI" 
COMMENT         "MspA1I" "MwoI" "NaeI" "NarI" "NciI" "NcoI" "NdeI" "NgoMIV" "NheI" 
COMMENT         "NlaIII" "NlaIV" "Nli3877I" "NotI" "NruI" "NsiI" "NspI" "PacI" "PciI" 
COMMENT         "PflMI" "PfoI" "PleI" "PmeI" "PmlI" "PpiI" "Ppu10I" "PpuMI" "PshAI" 
COMMENT         "PsiI" "Psp03I" "PspGI" "PspOMI" "PsrI" "PssI" "PstI" "PvuI" "PvuII" 
COMMENT         "RleAI" "RsaI" "RsrII" "SacI" "SacII" "SalI" "SanDI" "SapI" "Sau96I" 
COMMENT         "SbfI" "ScaI" "SciI" "ScrFI" "SelI" "SexAI" "SfaNI" "SfcI" "SfiI" 
COMMENT         "SfoI" "SgrAI" "SimI" "SmaI" "SmlI" "SnaBI" "SpeI" "SphI" "SrfI" 
COMMENT         "Sse232I" "Sse8647I" "SspD5I" "SspI" "Sth132I" "StsI" "StuI" "StyD4I" 
COMMENT         "StyI" "SwaI" "TaiI" "TaqI" "TaqII" "TatI" "TauI" "TfiI" "TseI" 
COMMENT         "Tsp45I" "Tsp509I" "TspDTI" "TspGWI" "TspRI" "Tth111I" "Tth111II" 
COMMENT         "UnbI" "UthSI" "VpaK11AI" "XbaI" "XcmI" "XhoI" "XmaI" "XmnI" "ZraI") 
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COMMENT                          "T7 driven prokaryotic expression vector (Invitrogen)." 
COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: Alex Borovkov" 
COMMENT                          1) 
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COMMENT                          "Created: 05/29/12 10:26AM" 1) 
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COMMENT                          "Last Modified: 12/03/19 01:03PM" 1) 
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FEATURES             Location/Qualifiers
     promoter        20..39
                     /vntifkey="30"
                     /label=T7\promoter
     rep_origin      1650..2105
                     /vntifkey="33"
                     /label=f1\ori
     CDS             2236..3096
                     /vntifkey="4"
                     /label=AMP-R
     promoter        2137..2235
                     /vntifkey="30"
                     /label=bla\pr
     rep_origin      3241..3914
                     /vntifkey="33"
                     /label=pUC\ori
     RBS             85..92
                     /vntifkey="32"
                     /label=RBS
     terminator      1450..1579
                     /vntifkey="43"
                     /label=T7\term
     CDS             100..1398
                     /vntifkey="4"
                     /label=EXPRESSED\BBA57-TEV-His12
     misc_feature    1342..1362
                     /vntifkey="21"
                     /label=TEV\protease\cleavage\ENLYFQ*G
     misc_feature    1363..1398
                     /vntifkey="21"
                     /label=His12
     misc_feature    1399..1404
                     /vntifkey="21"
                     /label=double\stop\codon
     CDS             100..1341
                     /vntifkey="4"
                     /label=BBA57\AAC66270.1,\expression\optimized\DNA
     primer          20..39
                     /vntifkey="27"
                     /label=PRIMER\T7
     primer          522..545
                     /vntifkey="27"
                     /label=PRIMER\A57\seq\F
     primer          complement(1492..1510)
                     /vntifkey="27"
                     /label=PRIMER\T7-Ter
BASE COUNT     1176 a      1011 c      1004 g       900 t 
ORIGIN
        1 gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct 
       61 agaaataatt ttgtttaact ttaagaagga gatatacata tgaacggcaa actgcgtaag 
      121 gcgctgaaaa tcgcgatttt caccaccctg ctgctggtga tcagctgcaa cgcgaacatg 
      181 gataccaacg acaaaaacaa ggcgctgaac gagtataaac tgaagaacat tagcgaagtt 
      241 atcaagaaca gcctgcagct ggagagcgac ccgaaactga agaaagagcc ggaaagcaac 
      301 atcaaccaaa gcaccccgcc gatcctggag attgaaaaaa tcgagccggg taaacaggaa 
      361 atgagcctga agagcgagtt cggcagcgaa agcctgatgc cgctggaaga gccggaagag 
      421 gcgaacatgg cgaaaagcga agaggaaatt gcgaaaatcc aggagaagct gctgctgatt 
      481 ggtgcgagcg atgaaatcac cgaccaggag ctgggcgaaa acatgcaaaa gtttctgaac 
      541 ccgaccaccg ttgagttcaa aatcagcacc accaccaacg aaaccattct gaccaccatc 
      601 gaggaagagg aaatcaacaa caacaaggat aagatcttcg accacaaaga ggagaacgtg 
      661 accctgggta acaacagcct ggagaacgcg accctgaaca agaacaccat taccctggcg 
      721 cagaaccaaa aatataccac ccacctgaaa aacgacgata agtttatcac caaagaatac 
      781 ctgaagcaag ttcgtgatag cctggacaag gcgctgaacg cgattaaaaa cctggagacc 
      841 agcaaggagt tccgtgagct ggaaaacctg ccgaaagatc aggagaacag cccgagcgcg 
      901 aagaaagaga gcaccgaaag caacattaac aacaccaaca ccgagaacat cgaaatcatt 
      961 aaaaaccgtc tgctggagga actgaacaag agcgagctga tcttcgaaga taccaaagac 
     1021 ccgctgggca ccagcaccat taagaaagtg atcgacgcgg cgaagaaatg gcaggagaag 
     1081 gaaaacagca gccaaatcga ctgggatctg ggcttcaaat ttcacccgaa cccgaaattt 
     1141 tataacaagc tgaccgcgca agagtacaaa gtgctggcgg aaaagttcac caaagttaag 
     1201 aacgagtaca agaacaccaa agagcagctg aaagcggaaa gcaagctgac caccaacagc 
     1261 atcagcaaaa tcattaacgc gaccaaggag ttcgcgaacc aagtgattaa cctgatcctg 
     1321 ctggttgaga agaactacca ggaaaatctt tattttcaag gtcaccatca tcaccaccat 
     1381 caccatcatc accaccatta ataaaagggc gaattccagc acactggcgg ccgttactag 
     1441 tggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc 
     1501 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag 
     1561 gaggaactat atccggatct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca 
     1621 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc 
     1681 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc 
     1741 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa 
     1801 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact 
     1861 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt 
     1921 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa 
     1981 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt 
     2041 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac 
     2101 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 
     2161 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 
     2221 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 
     2281 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 
     2341 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 
     2401 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 
     2461 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat 
     2521 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 
     2581 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 
     2641 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 
     2701 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 
     2761 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa 
     2821 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 
     2881 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 
     2941 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 
     3001 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 
     3061 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 
     3121 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 
     3181 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 
     3241 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 
     3301 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 
     3361 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 
     3421 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 
     3481 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 
     3541 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 
     3601 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 
     3661 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 
     3721 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 
     3781 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 
     3841 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 
     3901 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc 
     3961 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg 
     4021 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt 
     4081 cattaatgca g 
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