LOCUS       pBB0405-TEV-His12        3458 bp    DNA     circular      3-DEC-2019
DEFINITION  T7 driven prokaryotic expression vector (Invitrogen).
SOURCE      
  ORGANISM  
COMMENT     Vector design and cloning by Debra T. Hansen and Felicia M. Craciunescu.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|627388011|
COMMENT     VNTDBDATE|-13106325|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pBB0405-TEV-His12|
COMMENT     VNTAUTHORNAME|Debra T Hansen|
COMMENT     VNTAUTHORTEL|000-000-0000|
COMMENT     VNTAUTHOREML|debra.hansen.1@asu.edu|
COMMENT     VNTOAUTHORNAME|Alex Borovkov|
COMMENT     VNTOAUTHOREML|ayboro@asu.edu|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT         "AlwI" "AlwNI" "ApaBI" "ApaI" "ApaLI" "ApoI" "AscI" "AseI" "AsiSI" 
COMMENT         "AvaI" "AvaII" "AvrII" "BaeI" "BamHI" "BanI" "BanII" "BbeI" "Bbr7I" 
COMMENT         "BbsI" "BbvCI" "BbvI" "BccI" "BceAI" "BcefI" "BcgI" "BciVI" "BclI" 
COMMENT         "BfaI" "BfrBI" "BglI" "BglII" "BlpI" "Bme1580I" "BmgBI" "BmrI" "BmtI" 
COMMENT         "BplI" "BpmI" "Bpu10I" "BpuEI" "BsaAI" "BsaBI" "BsaHI" "BsaI" "BsaJI" 
COMMENT         "BsaWI" "BsaXI" "BscAI" "BseMII" "BseRI" "BseYI" "BsgI" "BsiEI" 
COMMENT         "BsiHKAI" "BsiWI" "BslI" "BsmAI" "BsmBI" "BsmFI" "BsmI" "Bsp1286I" 
COMMENT         "Bsp24I" "BspCNI" "BspEI" "BspHI" "BspMI" "BsrBI" "BsrDI" "BsrFI" 
COMMENT         "BsrGI" "BsrI" "BssHII" "BssSI" "BstAPI" "BstBI" "BstEII" "BstF5I" 
COMMENT         "BstKTI" "BstNI" "BstUI" "BstXI" "BstYI" "BstZ17I" "Bsu36I" "BtgI" 
COMMENT         "BthCI" "BtsI" "Cac8I" "CdiI" "ChaI" "CjeI" "CjePI" "ClaI" "Csp6I" 
COMMENT         "CviAII" "CviJI" "DdeI" "DpnI" "DraI" "DraIII" "DrdI" "EaeI" "EagI" 
COMMENT         "EarI" "EciI" "Eco57MI" "EcoHI" "EcoICRI" "EcoNI" "EcoO109I" "EcoRI" 
COMMENT         "EcoRV" "EsaBC3I" "FalI" "FatI" "FauI" "FmuI" "Fnu4HI" "FokI" "FseI" 
COMMENT         "FspAI" "FspI" "GdiII" "HaeI" "HaeII" "HaeIII" "HaeIV" "HgaI" "HhaI" 
COMMENT         "Hin4I" "HincII" "HindIII" "HinfI" "HinP1I" "HpaI" "HpaII" "HphI" 
COMMENT         "Hpy188I" "Hpy188III" "Hpy8I" "Hpy99I" "HpyAV" "HpyCH4I" "HpyCH4III" 
COMMENT         "HpyCH4IV" "HpyCH4V" "HpyF10VI" "KasI" "KpnI" "LpnI" "MaeIII" "MboI" 
COMMENT         "MboII" "MfeI" "MluI" "MlyI" "MmeI" "MnlI" "MscI" "MseI" "MslI" 
COMMENT         "MspA1I" "MwoI" "NaeI" "NarI" "NciI" "NcoI" "NdeI" "NgoMIV" "NheI" 
COMMENT         "NlaIII" "NlaIV" "Nli3877I" "NotI" "NruI" "NsiI" "NspI" "PacI" "PciI" 
COMMENT         "PflMI" "PfoI" "PleI" "PmeI" "PmlI" "PpiI" "Ppu10I" "PpuMI" "PshAI" 
COMMENT         "PsiI" "Psp03I" "PspGI" "PspOMI" "PsrI" "PssI" "PstI" "PvuI" "PvuII" 
COMMENT         "RleAI" "RsaI" "RsrII" "SacI" "SacII" "SalI" "SanDI" "SapI" "Sau96I" 
COMMENT         "SbfI" "ScaI" "SciI" "ScrFI" "SelI" "SexAI" "SfaNI" "SfcI" "SfiI" 
COMMENT         "SfoI" "SgrAI" "SimI" "SmaI" "SmlI" "SnaBI" "SpeI" "SphI" "SrfI" 
COMMENT         "Sse232I" "Sse8647I" "SspD5I" "SspI" "Sth132I" "StsI" "StuI" "StyD4I" 
COMMENT         "StyI" "SwaI" "TaiI" "TaqI" "TaqII" "TatI" "TauI" "TfiI" "TseI" 
COMMENT         "Tsp45I" "Tsp509I" "TspDTI" "TspGWI" "TspRI" "Tth111I" "Tth111II" 
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COMMENT                          "T7 driven prokaryotic expression vector (Invitrogen)." 
COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: Alex Borovkov" 
COMMENT                          1) 
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COMMENT                          "Created: 05/29/12 10:26AM" 1) 
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COMMENT                          "Last Modified: 12/03/19 01:00PM" 1) 
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FEATURES             Location/Qualifiers
     promoter        20..39
                     /vntifkey="30"
                     /label=T7\promoter
     rep_origin      1017..1472
                     /vntifkey="33"
                     /label=f1\ori
     CDS             1603..2463
                     /vntifkey="4"
                     /label=AMP-R
     promoter        1504..1602
                     /vntifkey="30"
                     /label=bla\pr
     rep_origin      2608..3281
                     /vntifkey="33"
                     /label=pUC\ori
     RBS             85..92
                     /vntifkey="32"
                     /label=RBS
     terminator      817..946
                     /vntifkey="43"
                     /label=T7\term
     CDS             100..765
                     /vntifkey="4"
                     /label=EXPRESSED\BB0405-TEV-His12
     misc_feature    709..729
                     /vntifkey="21"
                     /label=TEV\protease\cleavage\ENLYFQ*G
     misc_feature    730..765
                     /vntifkey="21"
                     /label=His12
     misc_feature    766..771
                     /vntifkey="21"
                     /label=double\stop\codon
     primer          20..39
                     /vntifkey="27"
                     /label=PRIMER\T7
     primer          complement(859..877)
                     /vntifkey="27"
                     /label=PRIMER\T7-Ter
     CDS             100..729
                     /vntifkey="4"
                     /label=BB0405\AAC66795.1,\expression\optimized\gene
BASE COUNT      857 a       847 c       882 g       872 t 
ORIGIN
        1 gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct 
       61 agaaataatt ttgtttaact ttaagaagga gatatacata tgcgtatgct gctggcgacc 
      121 atcattctga ttctgaccac cggtctgctg gcggcgcaga gcaaaagcaa gagcatgacc 
      181 gaggacgatt tcgactttga taaactgctg gcgaaagagg agagcgtgcg tcgtctgttt 
      241 ggcatcggtt tcggcgttgg ttacccgctg gcgaacatca ccattagcgt gccgtatgtt 
      301 gacattgatc tgggttacgg tggctttgtg ggcctgaagc cgaacaactt cctgccgtat 
      361 gtggttatgg gtgttgatct gctgttcaaa gacgaaattc acaagaacac catgatcagc 
      421 ggtggcatcg gcattggtgc ggactggagc aaaggcagcc cggagaaaag caacgaaaag 
      481 ctggaggaag aggaagagaa cgaggcgcag caagtggcga gcctgcagaa ccgtattggt 
      541 gtggttatcc gtctgccgct ggttattgaa tacagctttc tgaaaaacat cgtgattggc 
      601 ttcaaggcgg ttgcgaccat cggcaccacc atgctgctgg gcagcccgat gagctttgag 
      661 ggtgcgcgtt tcaactttct gggcaccggt ttcatcaaga tctacatcga aaatctttat 
      721 tttcaaggtc accatcatca ccaccatcac catcatcacc accattaata aaagggcgaa 
      781 ttccagcaca ctggcggccg ttactagtgg atccggctgc taacaaagcc cgaaaggaag 
      841 ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac 
      901 gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatctggc gtaatagcga 
      961 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc 
     1021 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac 
     1081 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt 
     1141 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc 
     1201 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc 
     1261 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact 
     1321 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg 
     1381 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc 
     1441 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc 
     1501 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa 
     1561 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc 
     1621 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa 
     1681 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa 
     1741 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg 
     1801 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa 
     1861 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc 
     1921 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc 
     1981 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta 
     2041 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag 
     2101 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca 
     2161 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata 
     2221 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc 
     2281 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca 
     2341 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca 
     2401 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg 
     2461 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa 
     2521 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt 
     2581 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 
     2641 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 
     2701 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 
     2761 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac 
     2821 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 
     2881 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 
     2941 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 
     3001 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag 
     3061 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 
     3121 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 
     3181 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 
     3241 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc 
     3301 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc 
     3361 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa 
     3421 ccgcctctcc ccgcgcgttg gccgattcat taatgcag 
//
