LOCUS       pelB-MBP-P66-ss        8297 bp    DNA     circular      3-DEC-2019
DEFINITION  pet26-MBP-ELIC.
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     TEV protease cleavage site is designed for use with ligation-independent cloning into the
            BseRI site.
            This TEV site contains only the amino acids ENLYFQ.
            Cleavage occurs after the Q, and requires a compatible amino acid C-terminal to the Q,
            such as S, G, A, M. See Kapust et al 2002 Biochem Biophys Res Commun 294:949.
            Vector designed by Debbie T. Hansen.
            Cloning performed by Felicia M. Craciunescu.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDBDATE|-13106348|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pelB-MBP-P66-ss|
COMMENT     VNTAUTHORNAME|Debra T Hansen|
COMMENT     VNTAUTHORTEL|000-000-0000|
COMMENT     VNTAUTHOREML|debra.hansen.1@asu.edu|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT         "AlwI" "AlwNI" "ApaBI" "ApaI" "ApaLI" "ApoI" "AscI" "AseI" "AsiSI" 
COMMENT         "AvaI" "AvaII" "AvrII" "BaeI" "BamHI" "BanI" "BanII" "BbeI" "Bbr7I" 
COMMENT         "BbsI" "BbvCI" "BbvI" "BccI" "BceAI" "BcefI" "BcgI" "BciVI" "BclI" 
COMMENT         "BfaI" "BfrBI" "BglI" "BglII" "BlpI" "Bme1580I" "BmgBI" "BmrI" "BmtI" 
COMMENT         "BplI" "BpmI" "Bpu10I" "BpuEI" "BsaAI" "BsaBI" "BsaHI" "BsaI" "BsaJI" 
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COMMENT         "BsiHKAI" "BsiWI" "BslI" "BsmAI" "BsmBI" "BsmFI" "BsmI" "Bsp1286I" 
COMMENT         "Bsp24I" "BspCNI" "BspEI" "BspHI" "BspMI" "BsrBI" "BsrDI" "BsrFI" 
COMMENT         "BsrGI" "BsrI" "BssHII" "BssSI" "BstAPI" "BstBI" "BstEII" "BstF5I" 
COMMENT         "BstKTI" "BstNI" "BstUI" "BstXI" "BstYI" "BstZ17I" "Bsu36I" "BtgI" 
COMMENT         "BthCI" "BtsI" "Cac8I" "CdiI" "ChaI" "CjeI" "CjePI" "ClaI" "Csp6I" 
COMMENT         "CviAII" "CviJI" "DdeI" "DpnI" "DraI" "DraIII" "DrdI" "EaeI" "EagI" 
COMMENT         "EarI" "EciI" "Eco57MI" "EcoHI" "EcoICRI" "EcoNI" "EcoO109I" "EcoRI" 
COMMENT         "EcoRV" "EsaBC3I" "FalI" "FatI" "FauI" "FmuI" "Fnu4HI" "FokI" "FseI" 
COMMENT         "FspAI" "FspI" "GdiII" "HaeI" "HaeII" "HaeIII" "HaeIV" "HgaI" "HhaI" 
COMMENT         "Hin4I" "HincII" "HindIII" "HinfI" "HinP1I" "HpaI" "HpaII" "HphI" 
COMMENT         "Hpy188I" "Hpy188III" "Hpy8I" "Hpy99I" "HpyAV" "HpyCH4I" "HpyCH4III" 
COMMENT         "HpyCH4IV" "HpyCH4V" "HpyF10VI" "KasI" "KpnI" "LpnI" "MaeIII" "MboI" 
COMMENT         "MboII" "MfeI" "MluI" "MlyI" "MmeI" "MnlI" "MscI" "MseI" "MslI" 
COMMENT         "MspA1I" "MwoI" "NaeI" "NarI" "NciI" "NcoI" "NdeI" "NgoMIV" "NheI" 
COMMENT         "NlaIII" "NlaIV" "Nli3877I" "NotI" "NruI" "NsiI" "NspI" "PacI" "PciI" 
COMMENT         "PflMI" "PfoI" "PleI" "PmeI" "PmlI" "PpiI" "Ppu10I" "PpuMI" "PshAI" 
COMMENT         "PsiI" "Psp03I" "PspGI" "PspOMI" "PsrI" "PssI" "PstI" "PvuI" "PvuII" 
COMMENT         "RleAI" "RsaI" "RsrII" "SacI" "SacII" "SalI" "SanDI" "SapI" "Sau96I" 
COMMENT         "SbfI" "ScaI" "SciI" "ScrFI" "SelI" "SexAI" "SfaNI" "SfcI" "SfiI" 
COMMENT         "SfoI" "SgrAI" "SimI" "SmaI" "SmlI" "SnaBI" "SpeI" "SphI" "SrfI" 
COMMENT         "Sse232I" "Sse8647I" "SspD5I" "SspI" "Sth132I" "StsI" "StuI" "StyD4I" 
COMMENT         "StyI" "SwaI" "TaiI" "TaqI" "TaqII" "TatI" "TauI" "TfiI" "TseI" 
COMMENT         "Tsp45I" "Tsp509I" "TspDTI" "TspGWI" "TspRI" "Tth111I" "Tth111II" 
COMMENT         "UnbI" "UthSI" "VpaK11AI" "XbaI" "XcmI" "XhoI" "XmaI" "XmnI" "ZraI") 
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COMMENT                          "Organism(s): <a href=\"ncbilink:taxonomy/other+sequences;+artificial+sequences;+vectors.\">other sequences; artificial sequences; vectors.(other sequences; artificial sequences; vectors.)</a>" 
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COMMENT                                   "@N" 1 -12.7504 -13.9386 0 -10 4.82044 
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FEATURES             Location/Qualifiers
     source          1..8297
                     /organism="pet26-MBP-ELIC"
                     /mol_type="other DNA"
                     /vntifkey="98"
     gene            complement(561..1376)
                     /gene="KanR2"
                     /vntifkey="60"
     rep_origin      1482..2101
                     /vntifkey="33"
                     /label=pBR322_origin
     primer          2500..2522
                     /vntifkey="27"
                     /label=PRIMER\pGEX\3
     misc_feature    complement(2516..2707)
                     /vntifkey="21"
                     /label=ROP
     gene            complement(3179..3454)
                     /gene="tet (611 - 336)"
                     /vntifkey="60"
     misc_feature    complement(3516..4607)
                     /vntifkey="21"
                     /label=lacI
     gene            complement(4699..4950)
                     /gene="tet (887 - 636)"
                     /vntifkey="60"
     promoter        4985..5003
                     /vntifkey="30"
                     /label=T7_promoter
     promoter        5003..5030
                     /vntifkey="30"
                     /label=lacO
     misc_feature    5048..5064
                     /vntifkey="21"
                     /label=RBS
     terminator      8170..1
                     /vntifkey="43"
                     /label=T7_terminator
     misc_feature    5073..5138
                     /vntifkey="21"
                     /label=pelB\signal\peptide\(M1-A22\AAA24848;\PAMA*)
     CDS             complement(564..1376)
                     /vntifkey="4"
                     /label=kanamycin-R
     misc_feature    5178..5207
                     /vntifkey="21"
                     /label=His10
     misc_feature    5214..6311
                     /vntifkey="21"
                     /label=MBP\(K27-T392\NP_290668)
     mutation        5114..5114
                     /vntifkey="62"
                     /label=C\to\T\removed\BseRI
     misc_feature    6321..6338
                     /vntifkey="21"
                     /label=TEV\protease\cleavage\ENFLYQ*
     CDS             6339..8129
                     /vntifkey="4"
                     /label=P66\(aa\22-618),\expression\optimized\gene
     primer          complement(8255..8276)
                     /vntifkey="27"
                     /label=PRIMER\pET26\pelB\seqR
     primer          6188..6212
                     /vntifkey="27"
                     /label=PRIMER\MBPpelBseqF
     primer          complement(8212..8230)
                     /vntifkey="27"
                     /label=PRIMER\T7-Ter
     primer          4985..5004
                     /vntifkey="27"
                     /label=PRIMER\T7
     CDS             5073..8129
                     /vntifkey="4"
                     /label=EXPRESSED\PROTEIN
     misc_feature    8130..8135
                     /vntifkey="21"
                     /label=double\stop\codon
     primer          6826..6846
                     /vntifkey="27"
                     /label=PRIMER\P66\seq\1\F
     primer          7386..7406
                     /vntifkey="27"
                     /label=PRIMER\P66\seq\2\F
BASE COUNT     2116 a      2195 c      2150 g      1836 t 
ORIGIN
        1 ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc 
       61 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt 
      121 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag 
      181 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt 
      241 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt 
      301 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt 
      361 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt 
      421 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttca ggtggcactt 
      481 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt 
      541 atccgctcat gaattaattc ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta 
      601 ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa 
      661 aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact 
      721 cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag 
      781 aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg catttctttc 
      841 cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa 
      901 ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct gttaaaagga 
      961 caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata 
     1021 ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc ggggatcgca 
     1081 gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc 
     1141 ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta 
     1201 cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa tcgatagatt 
     1261 gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc 
     1321 atgttggaat ttaatcgcgg cctagagcaa gacgtttccc gttgaatatg gctcataaca 
     1381 ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgacca aaatccctta 
     1441 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg 
     1501 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc 
     1561 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag 
     1621 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa 
     1681 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc 
     1741 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc 
     1801 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta 
     1861 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag 
     1921 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct 
     1981 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga 
     2041 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc 
     2101 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt 
     2161 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg 
     2221 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg 
     2281 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt 
     2341 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact 
     2401 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc 
     2461 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga 
     2521 ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt 
     2581 cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca 
     2641 gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt 
     2701 tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga 
     2761 aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac 
     2821 gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg 
     2881 gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc 
     2941 ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt 
     3001 acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc 
     3061 agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac 
     3121 cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggggccg 
     3181 ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga 
     3241 aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg 
     3301 cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta 
     3361 cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc 
     3421 accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct 
     3481 aatgagtgag ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 
     3541 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 
     3601 ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc 
     3661 accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga 
     3721 aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg 
     3781 tatcccacta ccgagatatc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt 
     3841 gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc 
     3901 agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct 
     3961 atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc 
     4021 gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga 
     4081 tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc 
     4141 tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg 
     4201 gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga 
     4261 ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg 
     4321 ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc 
     4381 agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt 
     4441 gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt 
     4501 ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg 
     4561 gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc 
     4621 tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg 
     4681 atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag 
     4741 gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc 
     4801 cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg 
     4861 gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt 
     4921 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg 
     4981 aaattaatac gactcactat aggggaattg tgagcggata acaattcccc tctagaaata 
     5041 attttgttta actttaagaa ggagatatac atatgaaata cctgctgccg accgctgctg 
     5101 ctggtctgct gcttctcgct gcccagccgg cgatggccat ggatatcgga attaattcgg 
     5161 atccgaattc gagctcacat caccatcacc atcaccatca ccatcacccc atgaaaatcg 
     5221 aagaaggtaa actggtaatc tggattaacg gcgataaagg ctataacggt ctcgctgaag 
     5281 tcggtaagaa attcgagaaa gataccggaa ttaaagtcac cgttgagcat ccggataaac 
     5341 tggaagagaa attcccacag gttgcggcaa ctggcgatgg ccctgacatt atcttctggg 
     5401 cacacgaccg ctttggtggc tacgctcaat ctggcctgtt ggctgaaatc accccggaca 
     5461 aagcgttcca ggacaagctg tatccgttta cctgggatgc cgtacgttac aacggcaagc 
     5521 tgattgctta cccgatcgct gttgaagcgt tatcgctgat ttataacaaa gatctgctgc 
     5581 cgaacccgcc aaaaacctgg gaagagatcc cggcgctgga taaagaactg aaagcgaaag 
     5641 gtaagagcgc gctgatgttc aacctgcaag aaccgtactt cacctggccg ctgattgctg 
     5701 ctgacggggg ttatgcgttc aagtatgaaa acggcaagta cgacattaaa gacgtgggcg 
     5761 tggataacgc tggcgcgaaa gcgggtctga ccttcctggt tgacctgatt aaaaacaaac 
     5821 acatgaatgc agacaccgat tactccatcg cagaagctgc ctttaataaa ggcgaaacag 
     5881 cgatgaccat caacggcccg tgggcatggt ccaacatcga caccagcaaa gtgaattatg 
     5941 gtgtaacggt actgccgacc ttcaagggtc aaccatccaa accgttcgtt ggcgtgctga 
     6001 gcgcaggtat taacgccgcc agtccgaaca aagagctggc aaaagagttc ctcgaaaact 
     6061 atctgctgac tgatgaaggt ctggaagcgg ttaataaaga caaaccgctg ggtgccgtag 
     6121 cgctgaagtc ttacgaggaa gagttggcga aagatccacg tattgccgcc actatggaaa 
     6181 acgcccagaa aggtgaaatc atgccgaaca tcccgcagat gtccgctttc tggtatgccg 
     6241 tgcgtactgc ggtgatcaac gccgccagcg gtcgtcagac tgtcgatgaa gccctgaaag 
     6301 acgcgcagac tccgggtagc gaaaacctgt acttccaagc ggatgcgctg aaagaaaagg 
     6361 acatcttcaa aattaacccg tggatgccga ccttcggttt tgagaacacc agcgaatttc 
     6421 gtctggacat ggatgagctg gtgccgggct tcgaaaacaa aagcaagatc accattaaac 
     6481 tgaagccgtt tgaggcgaac ccggaactgg gcaaggacga tccgttcagc gcgtacatta 
     6541 aagtggagga tctggcgctg aaggcggaag gtaagaaagg cgaccaattc aaaatcgatg 
     6601 ttggcgacat taccgcgcag atcaacatgt acgacttctt tatcaagatt agcaccatga 
     6661 ccgacttcga ttttaacaaa gagagcctgt tcagctttgc gccgatgacc ggttttaaga 
     6721 gcacctacta tggcttcccg agcaacgatc gtgcggttcg tggcaccatc ctggcgcgtg 
     6781 gcaccagcaa aaacatcggc accattcaac tgggctacaa actgccgaag ctggacctga 
     6841 cctttgcgat cggtggcacc ggcaccggta accgtaacca ggaaaacgac aaggataccc 
     6901 cgtacaacaa aacctatcag ggcattctgt atggtatcca agcgacctgg aaaccgatta 
     6961 agaacctgct ggatcagaac gaggacacca agagcgtgat cgcggagacc ccgtttgaac 
     7021 tgaacttcgg tctgagcggc gcgtatggta acgaaacctt taacaacagc agcatcacct 
     7081 acagcctgaa agataagagc gtggttggta acgacctgct gagcccgacc ctgagcaaca 
     7141 gcgcgattct ggcgagcttc ggtgcgaaat ataagctggg cctgaccaaa attaacgata 
     7201 agaacaccta cctgatcctg cagatgggca ccgattttgg tatcgacccg ttcgcgagcg 
     7261 actttagcat tttcggtcac atcagcaagg cggcgaactt caagaaagag accccgagcg 
     7321 atccgaacaa gaaagcggaa atctttgacc cgaacggtaa cgcgctgaat ttcagcaaaa 
     7381 acaccgagct gggcattgcg tttagcaccg gcgcgagcat cggtttcgcg tggaacaaag 
     7441 ataccggcga gaaggaaagc tgggcgatca agggcagcga tagctacagc acccgtctgt 
     7501 ttggtgaaca agacaagaaa agcggcgtgg cgctgggtat tagctatggc cagaacctgt 
     7561 accgtagcaa agacaccgag aaacgtctga agaccattag cgaaaacgcg ttccaaagcc 
     7621 tgaacgttga gatcagcagc tatgaagata acaagaaagg tatcatcaac ggtctgggct 
     7681 ggatcaccag cattggcctg tatgacattc tgcgtcagaa aagcgtggag aactacccga 
     7741 ccaccatcag cagcaccacc gagaacaacc agaccgaaca aagcagcacc agcaccaaga 
     7801 ccaccacccc gaacctgacc ttcgaggatg cgatgaaact gggtctggcg ctgtacctgg 
     7861 actatgcgat cccgattgcg agcatcagca ccgaagcgta cgtggttccg tatattggtg 
     7921 cgtacatcct gggcccgagc aacaagctga gcagcgatgc gaccaaaatc tacctgaaga 
     7981 ccggtctgag cctggagaaa ctgattcgtt ttaccaccat cagcctgggc tgggacagca 
     8041 acaacatcat tgaactggcg aacaagaaca ccaacaacgc ggcgattggt agcgcgtttc 
     8101 tgcaattcaa aatcgcgtac agcggtagct aataactcga gcaccaccac caccaccact 
     8161 gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc 
     8221 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag 
     8281 gaggaactat atccgga 
//
