LOCUS       pGL2313      6735 bp    DNA     circular UNA 05-Oct-2010
DEFINITION  Invitrogen MS/GW donor vector 221
FEATURES             Location/Qualifiers
     Gateway         570..668
                     /label=attL1
     5'UTR           679..873
                     /label="Actin 5' UTR"
     SAS             800^801
                     /label="Splice Leader Site"
     CDS             874..906
                     /cds_type=ORF
     misc_feature    907..1218
                     /feature_type="Rapamycin Binding Domain"
                     /label=FKBP12
     primer          complement(910..927)
                     /label=OL4102
     Linker          1219..1263
     misc_feature    1258..1277
                     /feature_type="RT PCR Primer"
                     /label=OL4691
     misc_feature    1264..1389
                     /feature_type="Cre Recombinase Dimer"
                     /label=Cre59
     misc_feature    complement(1315..1334)
                     /feature_type="RT PCR Primer"
                     /label=OL4692
     3'UTR           1390..2229
                     /label="Actin 3'UTR"
     Site            join(1565..1570,1565^1566)
                     /site_type="restriction site"
                     /note="Name: BamHI"
                     /note="Pattern: ggatcc"
                     /note="cut_0_on_positive_strand: 1526^1527"
                     /note="cut_0_on_negative_strand: 1530^1531"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=BamHI
     misc_feature    1571..2218
                     /feature_type=Insertion
                     /label="3'UTR Insertion site"
     misc_feature    2020^2021
                     /feature_type="PolyA Site"
                     /label="PolyA Site"
     Site            join(2219..2226,2220^2221)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 1547^1548"
                     /note="cut_0_on_negative_strand: 1551^1552"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
     5'UTR           2230..2427
                     /label="Beta Tubulin 5'UTR"
     SAS             2353
                     /label="Splice Leader Site"
     CDS             2428..2460
                     /label=ORF
     misc_feature    2461..2737
                     /feature_type="Rapamycin Binding Domain"
                     /label=FRB
     Linker          2738..2772
     misc_feature    2773..3627
                     /feature_type="Cre Recombinase Dimer"
                     /label=Cre60
     misc_feature    2796..2815
                     /feature_type="RT PCR Primer"
                     /label=OL4693
     misc_feature    complement(2851..2870)
                     /feature_type="RT PCR Primer"
                     /label=OL4694
     3'UTR           3628..3825
                     /label="Beta Tubulin 3'UTR"
     5'UTR           3847..4146
                     /label="BLAr 5'UTR"
     CDS             4147..4542
                     /label=BLAr
     primer          4523..4540
                     /label=OL4101
     3'UTR           4543..4842
                     /label="BLAr 3'UTR"
     Gateway         4858..4958
                     /label=attL2
     CDS             5129..5938
                     /label="Kanamycin Resistance"
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
      601 CTGTTCGTTG CAACAAATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
      661 AGCAGGCTTG ccCTCGAGGT TTTCGTCGCG CATCGGTGTA GGTCGAATAC CCTTGCCTTT
      721 CAGTGCGGGG TTTTTTCCTA TAAAGCATTT ATTGTCTCGA CGCTTCTCCT GCTTGGTTCT
      781 CTCTCGCACT TGCATGACAG ATTTCAAGAT ATCTTGCCTG TACACTCGTC ATTAAAAACA
      841 GCTTATTCCC GGTTATTTTT CTCAACGAAG AAAATGGCCC CTAAGAAGAA GAGAAAGGTG
      901 TCAAGAGGAG TGCAGGTGGA AACCATCTCC CCAGGAGACG GGCGCACCTT CCCCAAGCGC
      961 GGCCAGACCT GCGTGGTGCA CTACACCGGG ATGCTTGAAG ATGGAAAGAA ATTTGATTCC
     1021 TCCAGGGACA GAAACAAGCC CTTTAAGTTT ATGCTAGGCA AGCAGGAGGT GATCCGAGGC
     1081 TGGGAAGAAG GGGTTGCCCA GATGAGTGTG GGTCAGAGAG CCAAACTGAC TATATCTCCA
     1141 GATTATGCCT ATGGTGCCAC TGGGCACCCA GGCATCATCC CACCACATGC CACTCTCGTC
     1201 TTCGATGTGG AGCTTCTAAA ACTGGAAGCT AGCCCCAGCA ACCCCGGCGC CAGCAACGGC
     1261 TCCACCTCTG ATGAAGTCAG GAAGAACCTG ATGGACATGT TCAGGGACAG GCAGGCCTTC
     1321 TCTGAACACA CCTGGAAGAT GCTCCTGTCT GTGTGCAGAT CCTGGGCTGC CTGGTGCAAG
     1381 CTGAACTGAA GGTCCTCACC CGACTGGGCA GCTGGAGTGC AGAGAACTTT GCGCGACCGG
     1441 AAGACGCTAG TGAGATTGGG CACAGTGGAG CTAGCGTGCG CGCGGTAGCT TTGGCAGCAC
     1501 TGGGATTTTG TCCTCTGTAC TGCCTGGCTC TCGTGTTTAT TTTTGGTTAG AGTGTGCGTT
     1561 GCTCGGATCC CTGCCTGGTT CTCGTGCTTA TTTTTGGTTA GATGGTGCGT TGTTCTACTT
     1621 TCAATCATCG TTTTGTTTTG TTTTTCATAT CTGTTTTCCA AAGAACTCTG ATTGTCTTCA
     1681 TCCACCCGGT TTTCCCCGCC TGGTCTCGCT TTGAGATTGT TTTTATTCCT CTCCGGTTTT
     1741 TCCCCATTGG GATAAAAGTG CGTCCCGTGT GTCTACCCCT CCAGTCCCCA CAAGCGGCTC
     1801 AGATGTACAG AGCACCTGCT CTTCTCTGAC GTGCACTTTT TGCTCTTGCA GTCTTGCGCG
     1861 GCCTCTAGTC TCCCCTGCCA CGCACGGATG CTGACGCCAA GCAGCATTCT GCGCAGGAAA
     1921 GCGTGGTATC CCGACGAGGC AGATCGCGGG CTCGCATGTA CCGGCGACGG ACATCCTACA
     1981 CACATTATCC TCATGTAGAA CCTCAAAGCA AGGGAAGAAG AAAAGGGTTG ACACGTTGTT
     2041 ATGTTTTTTT TTGTGCCTGG GAGTAAGCGG GCGCCGATCT TCAAGTATCC TCTCAATTTC
     2101 ACTGGCAGGG CGCGAAGTGC GCGGGCTCCT CGCGTGCCGC CGCAATGTCA GTCGCGGCTC
     2161 TACCAGTGCT ACAGGGATGT CGGGTGGTGT CGTTGATGAT TTGGCATCAA GTGCACGCGC
     2221 GGCCGCTTTC TTCCATCTGC ACTCGCTCTG CGCTTCGTTT TCTTCTTCCC TTGCGCGCGC
     2281 GTGACGCCGC GCTCCTCACC TGCGCGCCCC GCCCCGACTT GGGTCCTCTC GTGCTGCAAC
     2341 GGACGCCGAC AGCTTCCTTC CACGCATTTC GTGCCTCCGC ACCGTTCTCC ACCGCCAGCG
     2401 CAGACTTGTA CATACGCACA CACCACCATG GCCCCTAAGA AGAAGAGAAA GGTGTCAAGA
     2461 ATCCTCTGGC ATGAGATGTG GCATGAAGGC CTGGAAGAGG CATCTCGTTT GTACTTTGGG
     2521 GAAAGGAACG TGAAAGGCAT GTTTGAGGTG CTGGAGCCCT TGCATGCTAT GATGGAACGG
     2581 GGCCCCCAGA CTCTGAAGGA AACATCCTTT AATCAGGCCT ATGGTCGAGA TTTAATGGAG
     2641 GCCCAAGAGT GGTGCAGGAA GTACATGAAA TCAGGGAATG TCAAGGACCT CCTCCAAGCC
     2701 TGGGACCTCT ATTATCATGT GTTCCGACGA ATCTCAGCTA GCCCCAGCAA CCCCGGCGCC
     2761 AGCAACGGCT CCAACAGGAA ATGGTTCCCT GCTGAACCTG AGGATGTGAG GGACTACCTC
     2821 CTGTACCTGC AAGCCAGAGG CCTGGCTGTG AAGACCATCC AACAGCACCT GGGCCAGCTC
     2881 AACATGCTGC ACAGGAGGTC TGGCCTGCCT CGCCCTTCTG ACTCCAATGC TGTGTCCCTG
     2941 GTGATGAGGA GAATCAGAAA GGAGAATGTG GATGCTGGGG AGAGAGCCAA GCAGGCCCTG
     3001 GCCTTTGAAC GCACTGACTT TGACCAAGTC AGATCCCTGA TGGAGAACTC TGACAGATGC
     3061 CAGGACATCA GGAACCTGGC CTTCCTGGGC ATTGCCTACA ACACCCTGCT GCGCATTGCC
     3121 GAAATTGCCA GAATCAGAGT GAAGGACATC TCCCGCACCG ATGGTGGGAG AATGCTGATC
     3181 CACATTGGCA GGACCAAGAC CCTGGTGTCC ACAGCTGGTG TGGAGAAGGC CCTGTCCCTG
     3241 GGGGTTACCA AGCTGGTGGA GAGATGGATC TCTGTGTCTG GTGTGGCTGA TGACCCCAAC
     3301 AACTACCTGT TCTGCCGGGT CAGAAAGAAT GGTGTGGCTG CCCCTTCTGC CACCTCCCAA
     3361 CTGTCCACCC GAGCCCTGGA AGGGATCTTT GAGGCCACCC ACCGCCTGAT CTATGGTGCC
     3421 AAGGATGACT CTGGGCAGAG ATACCTGGCC TGGTCTGGCC ACTCTGCCAG AGTGGGTGCT
     3481 GCCAGGGACA TGGCCAGGGC TGGTGTGTCC ATCCCTGAAA TCATGCAGGC TGGTGGCTGG
     3541 ACCAATGTGA ACATTGTGAT GAACTACATC AGAAACCTGG ACTCTGAGAC TGGGGCCATG
     3601 GTGAGGCTGC TAGAGGATGG GGACTGAAGT GTGCGTGAGT GTGCGCGTGC GTGTTGGTGC
     3661 GCACCGCGCT CGCTGTCCCC GCGGCGCACG CACACCTCCC TCGCTCTCTC CCCCTCTTTC
     3721 CGTGCCTTCT TTGTCTCCCT TCCTGTACGC TATTTTGGTT TCTTGTGCTT GCTTTATCTT
     3781 GTGGGCGGGT GTGGGCCCGA CCTGCACGTC TGTGACGGAG TGACGTCTAG AGAGCTCGGG
     3841 TCGACCACAC GTACACACGT ACACACACAC GTGCGCATGC TTGACGCATA CGCGACGAAT
     3901 TCGAAAGCTC ACCTCATTCC TCCCTCCTCA CACCATCATC GGCATCCATA GAGACACGTG
     3961 CGCGTAGAAC GATACAGCTC ACGCGCACAG AGAGGCGTGT TCTGGTCGTG CGCATCATCG
     4021 GCACAGCACT GGCGGACGAA ACTCGCACAC AGGCACGCCG CCTCCTTTCA CCCGTCATAG
     4081 ATAGTTGAAT TAGACGCCCT CCTCCTCCCT CATCATCGCC GTCGTCATCC GGGTCCGAGC
     4141 ACTAGTATGG CCAAGCCTTT GTCTCAAGAA GAATCCACCC TCATTGAAAG AGCAACGGCT
     4201 ACAATCAACA GCATCCCCAT CTCTGAAGAC TACAGCGTCG CCAGCGCAGC TCTCTCTAGC
     4261 GACGGCCGCA TCTTCACTGG TGTCAATGTA TATCATTTTA CTGGGGGACC TTGTGCAGAA
     4321 CTCGTGGTGC TGGGCACTGC TGCTGCTGCG GCAGCTGGCA ACCTGACTTG TATCGTCGCG
     4381 ATCGGAAATG AGAACAGGGG CATCTTGAGC CCCTGCGGAC GGTGCCGACA GGTGCTTCTC
     4441 GATCTGCATC CTGGGATCAA AGCCATAGTG AAGGACAGTG ATGGACAGCC GACGGCAGTT
     4501 GGGATTCGTG AATTGCTGCC CTCTGGTTAT GTGTGGGAGG GCTAAAGATC TTCTAGTGAT
     4561 CCCGCCCGCA CCCGCGCGCG CTCAGGCCGC GTGTCGTCGT GCTCTTTCCA TTTTTTTTTT
     4621 GTGTGTGGTG GTGATTTGAG CTGCTCTCCG TTGTGTGCTG GGGACCCTCC TTCCCTCGAT
     4681 CTCCTCGTGC GGGGTCTCCG ACGCGCAGAC GCGGTGCGTG CGAGCGTGCA CGTGCTGTCG
     4741 CCGTGGCTGT AGTTGCGAGC GGAGAGAGAG AGAGAGGGAG GGAGGGAGGG CAGAGGGCAG
     4801 AGGACATGCG GGTGGGAACG TGCACCGGCC TCGTCTCACG CAGGCCCGGG CGGTACCcTA
     4861 CCCAGCTTTC TTGTACAAAG TTGGCATTAT AAGAAAGCAT TGCTTATCAA TTTGTTGCAA
     4921 CGAACAGGTC ACTATCAGTC AAAATAAAAT CATTATTTGC CATCCAGCTG ATATCCCCTA
     4981 TAGTGAGTCG TATTACATGG TCATAGCTGT TTCCTGGCAG CTCTGGCCCG TGTCTCAAAA
     5041 TCTCTGATGT TACATTGCAC AAGATAAAAA TATATCATCA TGAACAATAA AACTGTCTGC
     5101 TTACATAAAC AGTAATACAA GGGGTGTTAT GAGCCATATT CAACGGGAAA CGTCGAGGCC
     5161 GCGATTAAAT TCCAACATGG ATGCTGATTT ATATGGGTAT AAATGGGCTC GCGATAATGT
     5221 CGGGCAATCA GGTGCGACAA TCTATCGCTT GTATGGGAAG CCCGATGCGC CAGAGTTGTT
     5281 TCTGAAACAT GGCAAAGGTA GCGTTGCCAA TGATGTTACA GATGAGATGG TCAGACTAAA
     5341 CTGGCTGACG GAATTTATGC CTCTTCCGAC CATCAAGCAT TTTATCCGTA CTCCTGATGA
     5401 TGCATGGTTA CTCACCACTG CGATCCCCGG AAAAACAGCA TTCCAGGTAT TAGAAGAATA
     5461 TCCTGATTCA GGTGAAAATA TTGTTGATGC GCTGGCAGTG TTCCTGCGCC GGTTGCATTC
     5521 GATTCCTGTT TGTAATTGTC CTTTTAACAG CGATCGCGTA TTTCGTCTCG CTCAGGCGCA
     5581 ATCACGAATG AATAACGGTT TGGTTGATGC GAGTGATTTT GATGACGAGC GTAATGGCTG
     5641 GCCTGTTGAA CAAGTCTGGA AAGAAATGCA TAAACTTTTG CCATTCTCAC CGGATTCAGT
     5701 CGTCACTCAT GGTGATTTCT CACTTGATAA CCTTATTTTT GACGAGGGGA AATTAATAGG
     5761 TTGTATTGAT GTTGGACGAG TCGGAATCGC AGACCGATAC CAGGATCTTG CCATCCTATG
     5821 GAACTGCCTC GGTGAGTTTT CTCCTTCATT ACAGAAACGG CTTTTTCAAA AATATGGTAT
     5881 TGATAATCCT GATATGAATA AATTGCAGTT TCATTTGATG CTCGATGAGT TTTTCTAATC
     5941 AGAATTGGTT AATTGGTTGT AACACTGGCA GAGCATTACG CTGACTTGAC GGGACGGCGC
     6001 AAGCTCATGA CCAAAATCCC TTAACGTGAG TTACGCGTCG TTCCACTGAG CGTCAGACCC
     6061 CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT
     6121 GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC
     6181 TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT
     6241 GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT
     6301 GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA
     6361 CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC
     6421 ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG
     6481 AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT
     6541 CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC
     6601 TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG
     6661 GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC
     6721 TTTTGCTCAC ATGTT
//
