LOCUS       pGL2314      5908 bp    DNA     circular UNA 01-APR-2009
DEFINITION  plasmid for L. major ISP cluster KO PURr
ACCESSION   pGL1160
KEYWORDS    3` & 5` flanks; ampicillin resistance; hygromycin resistance; mexicana.
SOURCE      Expression vector pRIBOTEX (japonica cultivar-group)
  ORGANISM  Expression vector pRIBOTEX
            Expression vector pRIBOTEX
            artificial sequences; vectors
FEATURES             Location/Qualifiers
     primer          537..552
                     /label="M13 F"
     Gateway         570..668
                     /label=attL1
     misc_feature    675..708
                     /feature_type="LoxP Site"
                     /label=LoxP
     misc_feature    709..895
                     /feature_type="Splice acceptor site"
                     /label="pX SL"
     misc_feature    896..925
                     /feature_type=Insertion
                     /label="GOI Insertion Site"
     protein_bind    932..1096
                     /label=6xHA
     misc_feature    1103..1136
                     /feature_type="LoxP Site"
                     /label=LoxP
     primer          1139..1162
                     /label=OL4065
     polyA_site      1145..1640
     primer          complement(1283..1297)
                     /label=OL2060
     misc_signal     1641..1846
                     /label="Splice Acceptor Site"
     CDS             1847..2443
                     /label=PAC
     Site            join(<2457..>2460,<2461..2461,2456^2457)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 380^381"
                     /note="cut_0_on_negative_strand: 384^385"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     misc_feature    2462..2649
                     /feature_type="Splice acceptor site"
                     /label=SAS
     CDS             2650..2735
                     /vntifkey="4"
                     /label="HASPB N-term"
     primer          complement(2727..2744)
                     /label=OL4290
     CDS             2753..3460
                     /vntifkey="21"
                     /label=mCherry
     Tag             3461..3478
                     /label="His Tag"
     Site            join(3489..>3490,complement(3491..>3494),3490^3491,complement(3490^3491))
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 1516^1517"
                     /note="cut_0_on_negative_strand: 1520^1521"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
     polyA_site      3500..4024
                     /label="Lmcpb Poly A"
     polyA_signal    3927..3941
                     /label=S1
     Gateway         4033..4131
                     /label=attL2
     primer          complement(4173..4189)
                     /label="M13 R"
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
      601 CTGTTCGTTG CAACAAATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
      661 AGCAGGCTTA GATCATAACT TCGTATAGCA TACATTATAC GAAGTTATAA TTGCCCGCTT
      721 TCCATTTCGT CACCTTCCGC CTCTCTCTCT CTCTCTCTCA CCATCTACGC GTGCACATCA
      781 TCAACTGTCT CTTGTCGGTG CTCACCACCC TCAACCACCC CTCACTTTCA AGGCTTCCCG
      841 AACGCACACA AAAGGCGTGA AAACCGCTCG CGTGTGTTGA GCCGTCCACC GTAGCCATAT
      901 GGAATTCAGT ACTGGTACCA TCGATACTAG TTACCCTTAC GATGTGCCTG ATTACGCGTA
      961 CCCATACGAC GTGCCAGACT ACGCATACCC GTACGATGTG CCCGATTACG CATACCCTTA
     1021 CGATGTGCCT GATTACGCGT ACCCATACGA CGTGCCAGAC TACGCATACC CGTACGATGT
     1081 GCCCGATTAC GCATGATCTA GAATAACTTC GTATAGCATA CATTATACGA AGTTATGGCT
     1141 GCAGCGCGTG GATGTCGCGC AGCGCAAGAC CCCGTGCGCC TGAAACGTGA AGGGAGGTCG
     1201 AGAAGCGTGC CGCATGAGGC CTTAAGGCAG GAAAGTGAAA AGAGCTCGAC GCGAGTGCAG
     1261 CACGCGCGAC ATCGAACAGG ACGGAATAGA CACTCAGCCC CTTCCCCTTT CAAAAACTGA
     1321 ATGGACGGAC ATCGTAACGC GCTTCTCTCC CCTCCACTCC CCCTGTCCCT GTCTCCTTGA
     1381 CGTGTGCTGC TCTCGTGAGC GACAGATGAA GCGAGGCGTA GAAGGAAAGG GGGGGGAGCG
     1441 AGGCCTGCAC ACACACACAC ACACACACAC ACACACACAC ACACACACAC ACACACACAC
     1501 ACACGAAAAG CAAGTTTGAG GCAGACGTGG TTTCACACGA TTCCTGTGCA AGAAGCGTGC
     1561 CTGCGTGACG GTGTGGCTGT GTATGCATGC GTGAAGGGTT ACCGCAACGA TGCGCAATGG
     1621 ACTCCCCCGC TTTCCATTTC GTCACCTTCC GCCTCTCTCT CTCTCTCTCT CACCATCTAC
     1681 GCGTGCACAT CATCAACTGT CTCTTGTCGG TGCTCACCAC CCTCAACCAC CCCTCACTTT
     1741 CAAGGCTTCC CGAACGCACA CAAAAGGCGT GAAAACCGCT CGCGTGTGTT GAGCCGTCCA
     1801 CCGTAGCCCT CCCCCTGTCC CCGGGGGATC CACTAGACGA CCTTCCATGA CCGAGTACAA
     1861 GCCCACGGTG CGCCTCGCCA CCCGCGACGA CGTCCCCCGG GCCGTACGCA CCCTCGCCGC
     1921 CGCGTTCGCC GACTACCCCG CCACGCGCCA CACCGTCGAC CCGGACCGCC ACATCGAGCG
     1981 GGTCACCGAG CTGCAAGAAC TCTTCCTCAC GCGCGTCGGG CTCGACATCG GCAAGGTGTG
     2041 GGTCGCGGAC GACGGCGCCG CGGTGGCGGT CTGGACCACG CCGGAGAGCG TCGAAGCGGG
     2101 GGCGGTGTTC GCCGAGATCG GCCCGCGCAT GGCCGAGTTG AGCGGTTCCC GGCTGGCCGC
     2161 GCAGCAACAG ATGGAAGGCC TCCTGGCGCC GCACCGGCCC AAGGAGCCCG CGTGGTTCCT
     2221 GGCCACCGTC GGCGTCTCGC CCGACCACCA GGGCAAGGGT CTGGGCAGCG CCGTCGTGCT
     2281 CCCCGGAGTG GAGGCGGCCG AGCGCGCCGG GGTGCCCGCC TTCCTGGAGA CCTCCGCGCC
     2341 CCGCAACCTC CCCTTCTACG AGCGGCTCGG CTTCACCGTC ACCGCCGACG TCGAGTGCCC
     2401 GAAGGACCGC GCGACCTGGT GCATGACCCG CAAGCCCGGT GCCTAGGCTA GCGCTCTCGA
     2461 GAATTGCCCG CTTTCCATTT CGTCACCTTC CGCCTCTCTC TCTCTCTCTC TCACCATCTA
     2521 CGCGTGCACA TCATCAACTG TCTCTTGTCG GTGCTCACCA CCCTCAACCA CCCCTCACTT
     2581 TCAAGGCTTC CCGAACGCAC ACAAAAGGCG TGAAAACCGC TCGCGGTGTG TTGAGCCGTC
     2641 CACCGTAGCT GATCCTATCT ATCTCCCCCG CTTATACACC ATGGGAAGCT CTTGCACGAA
     2701 GGACTCCGCA AAGGAGCCCC AGAAAAGTGC GGATGGATCT ATGGAGGATC CAATGGTTAG
     2761 TAAAGGAGAA GAAAATAACA TGGCAATCAT TAAGGAGTTC ATGAGATTCA AAGTTCACAT
     2821 GGAAGGTTCT GTAAATGGAC ATGAATTTGA AATAGAAGGT GAAGGAGAAG GAAGGCCTTA
     2881 TGAAGGAACC CAAACCGCGA AGCTAAAAGT TACTAAGGGT GGCCCATTAC CATTTGCATG
     2941 GGATATCCTT AGCCCTCAAT TCATGTATGG GTCAAAGGCT TATGTCAAGC ACCCCGCCGA
     3001 CATTCCAGAC TATCTAAAGT TATCTTTTCC CGAAGGGTTT AAGTGGGAGC GTGTGATGAA
     3061 CTTCGAAGAC GGTGGCGTGG TAACAGTGAC TCAGGATTCG TCCCTGCAAG ATGGTGAATT
     3121 TATCTACAAA GTCAAATTAA GAGGAACTAA CTTTCCATCT GGCGGCCCGG TTATGCAAAA
     3181 AAAGACAATG GGCTGGGAGG CCTCCTCAGA ACGAATGTAC CCTGAAGATG GTGCCTTGAA
     3241 GGGTGAGATT AAACAAAGAT TGAAATTGAA AGATGGTGGA CATTATGACG CTGAGGTTAA
     3301 AACGACATAC AAAGCTAAGA AACCTGTCCA GCTCCCAGGT GCTTACAATG TAAATATAAA
     3361 ACTTGATATT ACATCACATA ATGAAGATTA TACGATAGTT GAACAATACG AAAGGGCTGA
     3421 GGGGAGACAT AGTACTGGTG GCATGGATGA ACTATACAAA CATCATCATC ATCATCATTG
     3481 AGGATCCCGC GGCCGCTAGA TGTGTGCCCT TGTGTGCGTG TGTGTTTGGT GGTTTGCAGC
     3541 GATGCCCGGC GCGTGTGGGC ACCTCCTTGG GTGCGCGCCC GCCGTGGCAG CTGCGCGTGC
     3601 GTGCGAGATG TGAGGCAGAG GAAGAGGAAG GCGATGCGGG CGACAGCGCA GCGAGGTGCG
     3661 GCGGAGCGTA GGGGGGAAAT GGACGAGCAG GCGCGCTGTG AATCGGAGCT GCGGCACCAC
     3721 CCAAGTCGTG GTGCCCCGCG AATGGCTGTT CTGCCCGCCC TCGCTTCACG CCTCCCCCTC
     3781 CCCTCGCGTG CCCTCGCGTG GCCTCCCTTG TTATCCCTCT CTCTCGCACG CACACGCACA
     3841 CGCGTATACG CGAGCCCGCT ATTCTGCCTT CGTCTGGCTC TTTGTATTTT GCTTGCTTCT
     3901 TCAGCACACT TGTGTGCTGT GCGTTCAGCG ATATCTTCCA CTACTTTGTT TTCTCCTCCC
     3961 CCTCGGGAGG TGCTTCGCTT GTGCTTTGAC GGTGGTGCGT GGCTGCTGGG TTATGTGCCG
     4021 GGCGCCCATG GAACCCAGCT TTCTTGTACA AAGTTGGCAT TATAAGAAAG CATTGCTTAT
     4081 CAATTTGTTG CAACGAACAG GTCACTATCA GTCAAAATAA AATCATTATT TGCCATCCAG
     4141 CTGATATCCC CTATAGTGAG TCGTATTACA TGGTCATAGC TGTTTCCTGG CAGCTCTGGC
     4201 CCGTGTCTCA AAATCTCTGA TGTTACATTG CACAAGATAA AAATATATCA TCATGAACAA
     4261 TAAAACTGTC TGCTTACATA AACAGTAATA CAAGGGGTGT TATGAGCCAT ATTCAACGGG
     4321 AAACGTCGAG GCCGCGATTA AATTCCAACA TGGATGCTGA TTTATATGGG TATAAATGGG
     4381 CTCGCGATAA TGTCGGGCAA TCAGGTGCGA CAATCTATCG CTTGTATGGG AAGCCCGATG
     4441 CGCCAGAGTT GTTTCTGAAA CATGGCAAAG GTAGCGTTGC CAATGATGTT ACAGATGAGA
     4501 TGGTCAGACT AAACTGGCTG ACGGAATTTA TGCCTCTTCC GACCATCAAG CATTTTATCC
     4561 GTACTCCTGA TGATGCATGG TTACTCACCA CTGCGATCCC CGGAAAAACA GCATTCCAGG
     4621 TATTAGAAGA ATATCCTGAT TCAGGTGAAA ATATTGTTGA TGCGCTGGCA GTGTTCCTGC
     4681 GCCGGTTGCA TTCGATTCCT GTTTGTAATT GTCCTTTTAA CAGCGATCGC GTATTTCGTC
     4741 TCGCTCAGGC GCAATCACGA ATGAATAACG GTTTGGTTGA TGCGAGTGAT TTTGATGACG
     4801 AGCGTAATGG CTGGCCTGTT GAACAAGTCT GGAAAGAAAT GCATAAACTT TTGCCATTCT
     4861 CACCGGATTC AGTCGTCACT CATGGTGATT TCTCACTTGA TAACCTTATT TTTGACGAGG
     4921 GGAAATTAAT AGGTTGTATT GATGTTGGAC GAGTCGGAAT CGCAGACCGA TACCAGGATC
     4981 TTGCCATCCT ATGGAACTGC CTCGGTGAGT TTTCTCCTTC ATTACAGAAA CGGCTTTTTC
     5041 AAAAATATGG TATTGATAAT CCTGATATGA ATAAATTGCA GTTTCATTTG ATGCTCGATG
     5101 AGTTTTTCTA ATCAGAATTG GTTAATTGGT TGTAACACTG GCAGAGCATT ACGCTGACTT
     5161 GACGGGACGG CGCAAGCTCA TGACCAAAAT CCCTTAACGT GAGTTACGCG TCGTTCCACT
     5221 GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG
     5281 TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC
     5341 AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA
     5401 CTGTTCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA
     5461 CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC
     5521 TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG
     5581 GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC
     5641 AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG
     5701 TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT
     5761 ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT
     5821 CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG
     5881 CCTTTTGCTG GCCTTTTGCT CACATGTT
//
