LOCUS       Exported                1970 bp ds-DNA     circular SYN 29-NOV-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   AG Stein
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Dec 2, 2019 from SnapGene Viewer 5.0.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1..22
                     /label=control fw on bb
     primer_bind     62..86
                     /label=pet 24 bb fw
     primer_bind     96..112
                     /label=linker cds mfe1 fw
     misc_feature    97..148
                     /label=gblock linker mcs
     primer_bind     144..166
                     /label=pL2 on Btsa1 fw
     primer_bind     complement(146..168)
                     /label=pL2 amplify rv
     primer_bind     complement(147..172)
                     /label=pL2 rv 2xG
     primer_bind     151..197
                     /label=pL2 SOC 2xG
     CDS             161..167
                     /codon_start=2
                     /label=linker
                     /translation="QW"
     misc_feature    167
                     /label=cutting site 1 1
     misc_feature    168
                     /label=cutting site 1 1
     primer_bind     173..192
                     /label=pL2 amplify fw
     primer_bind     216..238
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(269..294)
                     /label=pBR for Eco long
     primer_bind     complement(276..294)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward
                     primer"
     CDS             complement(427..1242)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1292..1319
                     /label=front of ori fw
     primer_bind     complement(1324..1344)
                     /label=front of ori rv
     rep_origin      1370..1958
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1859..1878
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
       61 ttatcccctg attctgtgga taaccgtatt accgccaatt ggctcgccgc agccgaacga
      121 ccgagcgcag cgagtcaccc gaagaccatt ttggacgcca gcagtgggtg ggcattgcat
      181 ccagccccga gcaaaaggag acccctgact agtctccggg agctgcatgt gtcagaggtt
      241 ttcaccgtca tcaccgaaac gcgcgacacg aaagggcctc gtgatacgcc tatttttata
      301 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggccttttcg gggaaatgtg
      361 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaat
      421 taattcttag aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt
      481 atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca
      541 gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat
      601 acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt
      661 gacgactgaa tccggtgaga atggcaaaag tttatgcatt tctttccaga cttgttcaac
      721 aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg
      781 tgattgcgcc tgagccagac gaaatacgcg gtcgctgtta aaaggacaat tacaaacagg
      841 aatcgaatgc aaccggcgca ggaacaccgc cagcgcatca acaatatttt cacctgaatc
      901 aggatattct tctaatacct ggaatgctgt tttccctggg atcgccgtgg tgagtaacca
      961 tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag
     1021 ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt
     1081 cagaaacaac tctggcgcat cgggcttccc atacaatcga tagattgtcg cacctgattg
     1141 cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa
     1201 tcgcggccta gagcaagacg tttcccgttg aatatggctc ataacacccc ttgtattact
     1261 gtttatgtaa gcagacagtt ttattgtgaa ttctcatgac caaaatccct taacgtgagt
     1321 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     1381 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     1441 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     1501 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     1561 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     1621 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     1681 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     1741 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     1801 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     1861 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     1921 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
//
