LOCUS       Exported                1972 bp ds-DNA     circular SYN 29-NOV-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   AG Stein
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Dec 2, 2019 from SnapGene Viewer 5.0.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     53..80
                     /label=front of ori fw
     primer_bind     complement(85..105)
                     /label=front of ori rv
     rep_origin      131..718
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     620..639
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     731..752
                     /label=control fw on bb
     primer_bind     complement(759..780)
                     /label=amp bb gblock2 rev
     primer_bind     826..842
                     /label=linker cds mfe1 fw
     misc_feature    827..878
                     /label=gblock linker mcs
     primer_bind     complement(876..898)
                     /label=pL2 amplify rv
     primer_bind     877..930
                     /label=pL2 SOC 1xP
     primer_bind     complement(882..905)
                     /label=pL2 rv 1xP
     CDS             891..898
                     /codon_start=2
                     /label=linker
                     /translation="QW"
     misc_feature    897..898
                     /label=cutting site 1 1
     CDS             900..902
                     /codon_start=1
                     /label=polyP linker
                     /translation="P"
     primer_bind     906..925
                     /label=pL2 amplify fw
     primer_bind     949..971
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1002..1027)
                     /label=pBR for Eco long
     primer_bind     complement(1009..1027)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward
                     primer"
     CDS             complement(join(1160..1972,1..3))
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 cataacaccc cttgtattac tgtttatgta agcagacagt tttattgtga attctcatga
       61 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
      121 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
      181 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
      241 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
      301 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
      361 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
      421 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
      481 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
      541 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
      601 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
      661 acctctgact tgagcgtcga tttttgtgat ctcgtcaggg gggcggagcc tatggaaaaa
      721 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
      781 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccaatt ggctcgccgc
      841 agccgaacga ccgagcgcag cgagtcaccc gaagaccatt ttggacgcca gcagtgggtc
      901 cggggcattg catccagccc cgagcaaaag gagacccctg actagtctcc gggagctgca
      961 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgac acgaaagggc ctcgtgatac
     1021 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggccttt
     1081 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     1141 tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat
     1201 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
     1261 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
     1321 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
     1381 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc
     1441 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac
     1501 cgttattcat tcgtgattgc gcctgagcca gacgaaatac gcggtcgctg ttaaaaggac
     1561 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac cgccagcgca tcaacaatat
     1621 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttccct gggatcgccg
     1681 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
     1741 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac
     1801 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg
     1861 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
     1921 tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ct
//
