LOCUS       Exported                2008 bp ds-DNA     circular SYN 29-NOV-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   AG Stein
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Dec 2, 2019 from SnapGene Viewer 5.0.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     primer_bind     1..22
                     /label=control fw on bb
     primer_bind     complement(29..50)
                     /label=amp bb gblock2 rev
     primer_bind     96..112
                     /label=linker cds mfe1 fw
     misc_feature    97..148
                     /label=gblock linker mcs
     primer_bind     complement(125..169)
                     /label=link mcs 2.0 rv
     primer_bind     complement(146..168)
                     /label=pL2 amplify rv
     CDS             161..167
                     /codon_start=2
                     /label=linker
                     /translation="QW"
     misc_feature    167..168
                     /label=cutting site 1 1
     CDS             169..170
                     /codon_start=2
                     /label=linker
                     /translation=""
     primer_bind     212..231
                     /label=pL2 amplify fw
     primer_bind     255..277
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(315..333)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward
                     primer"
     CDS             complement(466..1281)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1331..1358
                     /label=front of ori fw
     primer_bind     complement(1363..1383)
                     /label=front of ori rv
     rep_origin      1409..1996
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1898..1917
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
       61 ttatcccctg attctgtgga taaccgtatt accgccaatt ggctcgccgc agccgaacga
      121 ccgagcgcag cgagtcaccc gaagaccatt ttggacgcca gcagtgggtg gatccggagg
      181 tagcggtgga agtggaggat ctggatcagg gcattgcatc cagccccgag caaaaggaga
      241 cccctgacta gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
      301 cgcgacacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
      361 ggtttcttag acgtcaggtg gccttttcgg ggaaatgtgc gcggaacccc tatttgttta
      421 tttttctaaa tacattcaaa tatgtatccg ctcatgaatt aattcttaga aaaactcatc
      481 gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa
      541 aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc
      601 ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc
      661 gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa
      721 tggcaaaagt ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc
      781 atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagccagacg
      841 aaatacgcgg tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag
      901 gaacaccgcc agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg
      961 gaatgctgtt ttccctggga tcgccgtggt gagtaaccat gcatcatcag gagtacggat
     1021 aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc
     1081 atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc
     1141 gggcttccca tacaatcgat agattgtcgc acctgattgc ccgacattat cgcgagccca
     1201 tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctag agcaagacgt
     1261 ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag cagacagttt
     1321 tattgtgaat tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     1381 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     1441 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     1501 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     1561 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     1621 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     1681 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     1741 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     1801 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     1861 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     1921 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatct cgtcaggggg
     1981 gcggagccta tggaaaaacg ccagcaac
//
