LOCUS       Exported                1990 bp ds-DNA     circular SYN 29-NOV-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   AG Stein
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Dec 2, 2019 from SnapGene Viewer 5.0.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     primer_bind     53..80
                     /label=front of ori fw
     primer_bind     complement(85..105)
                     /label=front of ori rv
     rep_origin      131..718
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     620..639
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     731..752
                     /label=control fw on bb
     primer_bind     complement(759..780)
                     /label=amp bb gblock2 rev
     primer_bind     826..842
                     /label=linker cds mfe1 fw
     misc_feature    827..878
                     /label=gblock linker mcs
     primer_bind     complement(876..898)
                     /label=pL2 amplify rv
     CDS             897..929
                     /codon_start=1
                     /label=Feature 4
                     /translation="GPPPPPPPGHC"
     primer_bind     924..943
                     /label=pL2 amplify fw
     primer_bind     967..989
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1020..1045)
                     /label=pBR for Eco long
     primer_bind     complement(1027..1045)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward
                     primer"
     CDS             complement(join(1178..1990,1..3))
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 cataacaccc cttgtattac tgtttatgta agcagacagt tttattgtga attctcatga
       61 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
      121 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
      181 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
      241 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
      301 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
      361 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
      421 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
      481 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
      541 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
      601 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
      661 acctctgact tgagcgtcga tttttgtgat ctcgtcaggg gggcggagcc tatggaaaaa
      721 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
      781 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccaatt ggctcgccgc
      841 agccgaacga ccgagcgcag cgagtcaccc gaagaccatt ttggacgcca gcagtgggtc
      901 cgcctccacc acctccgcca gggcattgca tccagccccg agcaaaagga gacccctgac
      961 tagtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgacac
     1021 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     1081 agacgtcagg tggccttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     1141 aatacattca aatatgtatc cgctcatgaa ttaattctta gaaaaactca tcgagcatca
     1201 aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt
     1261 tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc
     1321 ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa
     1381 taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa
     1441 gtttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat
     1501 cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagccaga cgaaatacgc
     1561 ggtcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacaccg
     1621 ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg
     1681 ttttccctgg gatcgccgtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct
     1741 tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa
     1801 catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc
     1861 catacaatcg atagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc
     1921 catataaatc agcatccatg ttggaattta atcgcggcct agagcaagac gtttcccgtt
     1981 gaatatggct
//
