LOCUS       Exported                1997 bp ds-DNA     circular SYN 29-NOV-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   AG Stein
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Dec 2, 2019 from SnapGene Viewer 5.0.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1..22
                     /label=control fw on bb
     primer_bind     complement(29..50)
                     /label=amp bb gblock2 rev
     primer_bind     96..112
                     /label=linker cds mfe1 fw
     misc_feature    97..148
                     /label=gblock linker mcs
     primer_bind     complement(146..168)
                     /label=pL2 amplify rv
     CDS             161..168
                     /codon_start=2
                     /label=linker
                     /translation="QW"
     misc_feature    167..168
                     /label=cutting site 1 1
     misc_feature    170..196
                     /label=1xNLS Wilson poly PT
                     /note="Paper name: Engineered DNA ligases with improved 
                     activities in vitro"
     misc_feature    198
                     /label=cutting site 1 1
     primer_bind     200..219
                     /label=pL2 amplify fw
     primer_bind     243..265
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(303..321)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward
                     primer"
     CDS             complement(454..1269)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1319..1346
                     /label=front of ori fw
     primer_bind     complement(1351..1371)
                     /label=front of ori rv
     rep_origin      1397..1985
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1886..1905
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
       61 ttatcccctg attctgtgga taaccgtatt accgccaatt ggctcgccgc agccgaacga
      121 ccgagcgcag cgagtcaccc gaagaccatt ttggacgcca gcagtgggta cccctacgcc
      181 aactcctaca cccactgggc attgcatcca gccccgagca aaaggagacc cctgactagt
      241 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgacacgaaa
      301 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
      361 gtcaggtggc cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
      421 cattcaaata tgtatccgct catgaattaa ttcttagaaa aactcatcga gcatcaaatg
      481 aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg
      541 taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc
      601 tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag
      661 gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagttt
      721 atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact
      781 cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gccagacgaa atacgcggtc
      841 gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga acaccgccag
      901 cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt
      961 ccctgggatc gccgtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat
     1021 ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc
     1081 attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata
     1141 caatcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata
     1201 taaatcagca tccatgttgg aatttaatcg cggcctagag caagacgttt cccgttgaat
     1261 atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta ttgtgaattc
     1321 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1381 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1441 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1501 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     1561 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1621 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1681 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1741 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     1801 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     1861 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     1921 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     1981 ggaaaaacgc cagcaac
//
