LOCUS       Exported                1999 bp ds-DNA     circular SYN 29-NOV-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   AG Stein
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Dec 2, 2019 from SnapGene Viewer 5.0.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     primer_bind     1..22
                     /label=control fw on bb
     primer_bind     complement(29..50)
                     /label=amp bb gblock2 rev
     primer_bind     62..86
                     /label=pet 24 bb fw
                     /note="Nutzlich fur getrennten Gibson auf pET24"
     primer_bind     96..112
                     /label=linker cds mfe1 fw
     misc_feature    97..148
                     /label=gblock linker mcs
     primer_bind     complement(125..169)
                     /label=link mcs 2.0 rv
     primer_bind     144..166
                     /label=pL2 on Btsa1 fw
     primer_bind     complement(146..168)
                     /label=pL2 amplify rv
     CDS             167..202
                     /codon_start=1
                     /label=Feature 4
                     /translation="GGASPAAPAPAG"
     primer_bind     203..222
                     /label=pL2 amplify fw
     primer_bind     246..268
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(299..324)
                     /label=pBR for Eco long
     primer_bind     complement(306..324)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward
                     primer"
     CDS             complement(457..1272)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1322..1349
                     /label=front of ori fw
     primer_bind     complement(1354..1374)
                     /label=front of ori rv
     rep_origin      1400..1987
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1889..1908
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
       61 ttatcccctg attctgtgga taaccgtatt accgccaatt ggctcgccgc agccgaacga
      121 ccgagcgcag cgagtcaccc gaagaccatt ttggacgcca gcagtgggtg gagcatctcc
      181 tgcagctcca gctcctgcag ggcattgcat ccagccccga gcaaaaggag acccctgact
      241 agtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgacacg
      301 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
      361 gacgtcaggt ggccttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
      421 atacattcaa atatgtatcc gctcatgaat taattcttag aaaaactcat cgagcatcaa
      481 atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt
      541 ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg
      601 gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat
      661 aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag
      721 tttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc
      781 actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagccagac gaaatacgcg
      841 gtcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacaccgc
      901 cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt
      961 tttccctggg atcgccgtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt
     1021 gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac
     1081 atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc
     1141 atacaatcga tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc
     1201 atataaatca gcatccatgt tggaatttaa tcgcggccta gagcaagacg tttcccgttg
     1261 aatatggctc ataacacccc ttgtattact gtttatgtaa gcagacagtt ttattgtgaa
     1321 ttctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1381 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1441 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1501 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     1561 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1621 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1681 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1741 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1801 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1861 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1921 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatc tcgtcagggg ggcggagcct
     1981 atggaaaaac gccagcaac
//
