LOCUS       Exported                2014 bp ds-DNA     circular SYN 29-NOV-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   AG Stein
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Dec 2, 2019 from SnapGene Viewer 5.0.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(21..43)
                     /label=pL2 amplify rv
     CDS             36..42
                     /codon_start=2
                     /label=linker
                     /translation="QW"
     misc_feature    42..43
                     /label=cutting site 1 1
     CDS             44
                     /codon_start=2
                     /label=linker
                     /translation=""
     CDS             45..86
                     /codon_start=1
                     /label=Feature 2
                     /translation="LGGGSGGGSGGGSE"
     misc_feature    91
                     /label=cutting site 1 1
     primer_bind     93..112
                     /label=pL2 amplify fw
     primer_bind     136..158
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(196..214)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward
                     primer"
     CDS             complement(347..1162)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1212..1239
                     /label=front of ori fw
     primer_bind     complement(1244..1264)
                     /label=front of ori rv
     rep_origin      1290..1877
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1779..1798
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1890..1911
                     /label=control fw on bb
     primer_bind     complement(1918..1939)
                     /label=amp bb gblock2 rev
     primer_bind     1985..2001
                     /label=linker cds mfe1 fw
     misc_feature    join(1986..2014,1..23)
                     /label=gblock linker mcs
ORIGIN
        1 cgcagcgagt cacccgaaga ccattttgga cgccagcagt gggtcttgga ggtggatccg
       61 gtggcggatc cggaggcgga tccgagacag ggcattgcat ccagccccga gcaaaaggag
      121 acccctgact agtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
      181 gcgcgacacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
      241 tggtttctta gacgtcaggt ggccttttcg gggaaatgtg cgcggaaccc ctatttgttt
      301 atttttctaa atacattcaa atatgtatcc gctcatgaat taattcttag aaaaactcat
      361 cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa
      421 aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat
      481 cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct
      541 cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga
      601 atggcaaaag tttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt
      661 catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagccagac
      721 gaaatacgcg gtcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca
      781 ggaacaccgc cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct
      841 ggaatgctgt tttccctggg atcgccgtgg tgagtaacca tgcatcatca ggagtacgga
      901 taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct
      961 catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat
     1021 cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta tcgcgagccc
     1081 atttataccc atataaatca gcatccatgt tggaatttaa tcgcggccta gagcaagacg
     1141 tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa gcagacagtt
     1201 ttattgtgaa ttctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     1261 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     1321 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     1381 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     1441 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     1501 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     1561 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     1621 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     1681 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     1741 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     1801 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatc tcgtcagggg
     1861 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     1921 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     1981 ccgccaattg gctcgccgca gccgaacgac cgag
//
