LOCUS       Exported                7167 bp ds-DNA     circular SYN 25-NOV-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7167)
  AUTHORS   Charu
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Dec 2, 2019 from SnapGene Viewer 5.0.6
            https://www.snapgene.com
COMMENT     ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..7167
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..141
                     /label=L-ITR
                     /note="/vntifkey=21"
     promoter        156..1419
                     /label=EF-1a
                     /note="/vntifkey=29"
     misc_feature    1426..1459
                     /label=lox2722
                     /note="/vntifkey=21"
     misc_feature    1510..1543
                     /label=loxN
                     /note="/vntifkey=21"
     misc_feature    1550..1563
                     /label=CE donor
                     /label=CE\donor
                     /note="/vntifkey=21"
     misc_feature    complement(1564..1909)
                     /label=sRGECO Exon 1
                     /label=sRGECO\ex1
                     /note="/vntifkey=21"
     primer_bind     complement(1636..1655)
                     /label=sRGECO E1F
     misc_feature    1910..1915
                     /label=KOZAK
                     /note="/vntifkey=21"
     misc_feature    complement(1920..1953)
                     /label=lox 2722 Rev
                     /label=lox\2722\Rev
                     /note="/vntifkey=21"
     misc_feature    complement(2004..2037)
                     /label=lox N Rev
                     /label=lox\N\Rev
                     /note="/vntifkey=21"
     misc_feature    2044..2077
                     /label=F3
                     /note="/vntifkey=21"
     misc_feature    2128..2161
                     /label=F5
                     /note="/vntifkey=21"
     misc_feature    complement(2170..3077)
                     /label=sRGECO Exon 2
                     /label=sRGECO\ex2
                     /note="/vntifkey=21"
     primer_bind     2752..2771
                     /label=sRGECO E2R
     misc_feature    2773..2775
                     /label=E215D
                     /note="/vntifkey=21"
     misc_feature    complement(3078..3145)
                     /label=CE donor/acceptor
                     /label=CE\donor/acceptor
                     /note="/vntifkey=21"
     misc_feature    complement(3152..3185)
                     /label=F3 Rev
                     /label=F3\Rev
                     /note="/vntifkey=21"
     misc_feature    complement(3236..3269)
                     /label=F5 Rev
                     /label=F5\Rev
                     /note="/vntifkey=21"
     misc_feature    3284..3892
                     /label=WPRE
                     /note="/vntifkey=21"
     polyA_signal    3912..4390
                     /label=hGH polyA
                     /label=hGH\polyA
                     /note="/vntifkey=25"
     misc_feature    4430..4570
                     /label=R-ITR
                     /note="/vntifkey=21"
     rep_origin      4662..4968
                     /direction=RIGHT
                     /label=f1 Origin
                     /label=f1\Origin
                     /note="/vntifkey=33"
     misc_feature    5487..6344
                     /label=AmpR
                     /note="/vntifkey=21"
     rep_origin      6495..7162
                     /direction=RIGHT
                     /label=pUC ori
                     /label=pUC\ori
                     /note="/vntifkey=33"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaagct ttgcaaagat ggataaagtt
      181 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat
      241 tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
      301 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
      361 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
      421 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
      481 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
      541 tacttccact ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      601 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      661 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      721 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      781 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      841 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      901 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      961 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     1021 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     1081 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     1141 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     1201 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1261 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1321 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1381 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatccataac ttcgtatagg
     1441 atactttata cgaagttatg tagctggagc aatttgaaaa gattgttgta ttacctctag
     1501 ctgcgcttaa taacttcgta tagtatacct tatacgaagt tatgtcgacc ctcctgtact
     1561 cacctgtacc tcccttgtac agctcgtcca tgccgccggt ggagtggcgg ccctcggcgc
     1621 gttcgcactg ttccacgatg gtgtagtcct cgttgtggga cacgatgtcc aacttgatgt
     1681 ccacgatgta ggcgccgggc agctgcacgg gcttcttggc cttgtaggtg gtcttgacct
     1741 cggcggcgta gtggccgccg tccttcagcc tcagcccctt cttgatctcg ctcttcaggg
     1801 cgccgtcctc ggggtacatc cgctcggaaa ccacgggtga gctcagccga cctatagctc
     1861 tgactgcgtg acctgcctta ttccacttac gacgtgatga gtccaccatg gtggctagca
     1921 taacttcgta taaagtatcc tatacgaagt tatttgcctt aacccagaaa ttatcactgt
     1981 tattctttag ataggtgcaa agaataactt cgtataaggt atactatacg aagttatggt
     2041 accgaagttc ctattcttca aatagtatag gaacttcaat tgttattggt gcaaagaatc
     2101 acttccttaa gtctttatac tgcccaggaa gttcctattc ttcaaaaggt ataggaactt
     2161 cggcgcgccc tacttcgctg tcatcatttg tacaaactct tcgtagttta cctgaccatc
     2221 cccatcgatg tctgctaccc tgatcatttc atcaacctcc tcatctgtta acttctctcc
     2281 aaggtctgtc atcacgtggc gaagctctgc tgcgccgatg tagccattgc cgtccttatc
     2341 aaacacgcgg aacgcttcgc gaatttcctc ttcactgtct gtgtcattca tttttcttgc
     2401 catcatcgtc aggaactcag ggaagtcgaa tgtgccgtca ccgtcggcat ctacttcatt
     2461 gatcatgtcc tgcagctctg cttctgtggg gttctgcccc agcgatcgga acaccgtccc
     2521 aagctccttg gttgttatcg tcccatcccc gtccttgtca aatagggaga aagcctcttt
     2581 aaattctgcg atctgctctt cagtcaggtc gtcacgcgta gcctcccagc ccatggtctt
     2641 cttctgcatt acggggccgt cgggggggaa gttggtgccg cgcagcttca ccttgtagat
     2701 gaatacgccg tcctgcaggg aggagtcctg gttcacgtga ataatgccgc cgtcctcgaa
     2761 gttcatcacg cggtcccacc tgaagccctc ggggaaggac agcttgaagt agtcggggat
     2821 gtcggctggg tgcttaatgt aggccttgga gccgtacatg aactgagggg acaggatgtc
     2881 ccaggcgaag ggcagggggc cacccttggt caccttcagc ttagcggtct gaaaggcctc
     2941 gtaggggcgg ccctcgccct cgccctcgat ctcgaactcg tggccgttca cggagccctc
     3001 catgtgcacc ttgaagcgca tgaactcctt gatgatggcc atgttatcct cctcgccctt
     3061 gctcaccaga ctcccgcctg tgggacgaca tgacttaacc agttaaccat gttctctgaa
     3121 catgagaagg gctggccact ctccatctag agaagttcct atactatttg aagaatagga
     3181 acttcttgcc ttaacccaga aattatcact gttattcttt agaatagtgc aaagagaagt
     3241 tcctatacct tttgaagaat aggaacttcg aattcgatat caagcttatc gataatcaac
     3301 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     3361 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     3421 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     3481 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     3541 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     3601 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     3661 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctatg
     3721 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     3781 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     3841 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgagcgctgc
     3901 tcgagagatc tacgggtggc atccctgtga cccctcccca gtgcctctcc tggccctgga
     3961 agttgccact ccagtgccca ccagccttgt cctaataaaa ttaagttgca tcattttgtc
     4021 tgactaggtg tccttctata atattatggg gtggaggggg gtggtatgga gcaaggggca
     4081 agttgggaag acaacctgta gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt
     4141 ggcacaatct tggctcactg caatctccgc ctcctgggtt caagcgattc tcctgcctca
     4201 gcctcccgag ttgttgggat tccaggcatg catgaccagg ctcagctaat ttttgttttt
     4261 ttggtagaga cggggtttca ccatattggc caggctggtc tccaactcct aatctcaggt
     4321 gatctaccca ccttggcctc ccaaattgct gggattacag gcgtgaacca ctgctccctt
     4381 ccctgtcctt ctgattttgt aggtaaccac gtgcggaccg agcggccgca ggaaccccta
     4441 gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca
     4501 aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc
     4561 tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     4621 cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     4681 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     4741 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     4801 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     4861 tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     4921 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     4981 tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     5041 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt
     5101 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     5161 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     5221 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     5281 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     5341 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     5401 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     5461 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     5521 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     5581 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     5641 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     5701 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     5761 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     5821 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     5881 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     5941 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     6001 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     6061 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     6121 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     6181 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     6241 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     6301 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     6361 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     6421 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     6481 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     6541 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     6601 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     6661 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     6721 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     6781 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     6841 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     6901 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     6961 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     7021 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     7081 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     7141 ccttttgctg gccttttgct cacatgt
//
