LOCUS       Exported                7169 bp ds-DNA     circular SYN 25-NOV-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7169)
  AUTHORS   Charu
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Dec 2, 2019 from SnapGene Viewer 5.0.6
            https://www.snapgene.com
COMMENT     ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..7169
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..141
                     /label=L-ITR
                     /note="/vntifkey=21"
     promoter        156..1419
                     /label=EF-1a
                     /note="/vntifkey=29"
     misc_feature    1426..1459
                     /label=lox2722
                     /note="/vntifkey=21"
     misc_feature    1510..1543
                     /label=loxN
                     /note="/vntifkey=21"
     misc_feature    1550..1555
                     /label=KOZAK
                     /note="/vntifkey=21"
     misc_feature    1556..1901
                     /label=sRGECO Exon 1
                     /label=sRGECO\ex1
                     /note="/vntifkey=21"
     misc_feature    1902..1915
                     /label=CE donor
                     /label=CE\donor
                     /note="/vntifkey=21"
     misc_feature    complement(1922..1955)
                     /label=lox 2722 Rev
                     /label=lox\2722\Rev
                     /note="/vntifkey=21"
     misc_feature    complement(2006..2039)
                     /label=lox N Rev
                     /label=lox\N\Rev
                     /note="/vntifkey=21"
     misc_feature    2046..2079
                     /label=F3
                     /note="/vntifkey=21"
     misc_feature    2130..2163
                     /label=F5
                     /note="/vntifkey=21"
     misc_feature    complement(2172..3079)
                     /label=sRGECO Exon 2
                     /label=sRGECO\ex2
                     /note="/vntifkey=21"
     misc_feature    2775..2777
                     /label=E215D
                     /note="/vntifkey=21"
     misc_feature    complement(3080..3147)
                     /label=CE donor/acceptor
                     /label=CE\donor/acceptor
                     /note="/vntifkey=21"
     misc_feature    complement(3154..3187)
                     /label=F3 Rev
                     /label=F3\Rev
                     /note="/vntifkey=21"
     misc_feature    complement(3238..3271)
                     /label=F5 Rev
                     /label=F5\Rev
                     /note="/vntifkey=21"
     misc_feature    3286..3894
                     /label=WPRE
                     /note="/vntifkey=21"
     polyA_signal    3914..4392
                     /label=hGH polyA
                     /label=hGH\polyA
                     /note="/vntifkey=25"
     misc_feature    4432..4572
                     /label=R-ITR
                     /note="/vntifkey=21"
     rep_origin      4664..4970
                     /direction=RIGHT
                     /label=f1 Origin
                     /label=f1\Origin
                     /note="/vntifkey=33"
     misc_feature    5489..6346
                     /label=AmpR
                     /note="/vntifkey=21"
     rep_origin      6497..7164
                     /direction=RIGHT
                     /label=pUC ori
                     /label=pUC\ori
                     /note="/vntifkey=33"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaagct ttgcaaagat ggataaagtt
      181 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat
      241 tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
      301 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
      361 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
      421 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
      481 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
      541 tacttccact ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      601 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      661 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      721 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      781 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      841 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      901 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      961 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     1021 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     1081 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     1141 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     1201 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1261 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1321 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1381 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatccataac ttcgtatagg
     1441 atactttata cgaagttatg tagctggagc aatttgaaaa gattgttgta ttacctctag
     1501 ctgcgcttaa taacttcgta tagtatacct tatacgaagt tatgtcgacg ccaccatggt
     1561 ggactcatca cgtcgtaagt ggaataaggc aggtcacgca gtcagagcta taggtcggct
     1621 gagctcaccc gtggtttccg agcggatgta ccccgaggac ggcgccctga agagcgagat
     1681 caagaagggg ctgaggctga aggacggcgg ccactacgcc gccgaggtca agaccaccta
     1741 caaggccaag aagcccgtgc agctgcccgg cgcctacatc gtggacatca agttggacat
     1801 cgtgtcccac aacgaggact acaccatcgt ggaacagtgc gaacgcgccg agggccgcca
     1861 ctccaccggc ggcatggacg agctgtacaa gggaggtaca ggtgagtaca ggagggctag
     1921 cataacttcg tataaagtat cctatacgaa gttatttgcc ttaacccaga aattatcact
     1981 gttattcttt agataggtgc aaagaataac ttcgtataag gtatactata cgaagttatg
     2041 gtaccgaagt tcctattctt caaatagtat aggaacttca attgttattg gtgcaaagaa
     2101 tcacttcctt aagtctttat actgcccagg aagttcctat tcttcaaaag gtataggaac
     2161 ttcggcgcgc cctacttcgc tgtcatcatt tgtacaaact cttcgtagtt tacctgacca
     2221 tccccatcga tgtctgctac cctgatcatt tcatcaacct cctcatctgt taacttctct
     2281 ccaaggtctg tcatcacgtg gcgaagctct gctgcgccga tgtagccatt gccgtcctta
     2341 tcaaacacgc ggaacgcttc gcgaatttcc tcttcactgt ctgtgtcatt catttttctt
     2401 gccatcatcg tcaggaactc agggaagtcg aatgtgccgt caccgtcggc atctacttca
     2461 ttgatcatgt cctgcagctc tgcttctgtg gggttctgcc ccagcgatcg gaacaccgtc
     2521 ccaagctcct tggttgttat cgtcccatcc ccgtccttgt caaataggga gaaagcctct
     2581 ttaaattctg cgatctgctc ttcagtcagg tcgtcacgcg tagcctccca gcccatggtc
     2641 ttcttctgca ttacggggcc gtcggggggg aagttggtgc cgcgcagctt caccttgtag
     2701 atgaatacgc cgtcctgcag ggaggagtcc tggttcacgt gaataatgcc gccgtcctcg
     2761 aagttcatca cgcggtccca cctgaagccc tcggggaagg acagcttgaa gtagtcgggg
     2821 atgtcggctg ggtgcttaat gtaggccttg gagccgtaca tgaactgagg ggacaggatg
     2881 tcccaggcga agggcagggg gccacccttg gtcaccttca gcttagcggt ctgaaaggcc
     2941 tcgtaggggc ggccctcgcc ctcgccctcg atctcgaact cgtggccgtt cacggagccc
     3001 tccatgtgca ccttgaagcg catgaactcc ttgatgatgg ccatgttatc ctcctcgccc
     3061 ttgctcacca gactcccgcc tgtgggacga catgacttaa ccagttaacc atgttctctg
     3121 aacatgagaa gggctggcca ctctccatct agagaagttc ctatactatt tgaagaatag
     3181 gaacttcttg ccttaaccca gaaattatca ctgttattct ttagaatagt gcaaagagaa
     3241 gttcctatac cttttgaaga ataggaactt cgaattcgat atcaagctta tcgataatca
     3301 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     3361 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     3421 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     3481 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     3541 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     3601 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     3661 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgccta
     3721 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     3781 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     3841 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgagcgct
     3901 gctcgagaga tctacgggtg gcatccctgt gacccctccc cagtgcctct cctggccctg
     3961 gaagttgcca ctccagtgcc caccagcctt gtcctaataa aattaagttg catcattttg
     4021 tctgactagg tgtccttcta taatattatg gggtggaggg gggtggtatg gagcaagggg
     4081 caagttggga agacaacctg tagggcctgc ggggtctatt gggaaccaag ctggagtgca
     4141 gtggcacaat cttggctcac tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct
     4201 cagcctcccg agttgttggg attccaggca tgcatgacca ggctcagcta atttttgttt
     4261 ttttggtaga gacggggttt caccatattg gccaggctgg tctccaactc ctaatctcag
     4321 gtgatctacc caccttggcc tcccaaattg ctgggattac aggcgtgaac cactgctccc
     4381 ttccctgtcc ttctgatttt gtaggtaacc acgtgcggac cgagcggccg caggaacccc
     4441 tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac
     4501 caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca
     4561 gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     4621 accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg
     4681 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     4741 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     4801 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     4861 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     4921 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     4981 tatctcgggc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     5041 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
     5101 tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     5161 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     5221 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     5281 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     5341 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     5401 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     5461 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     5521 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     5581 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     5641 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     5701 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     5761 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     5821 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     5881 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     5941 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     6001 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     6061 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     6121 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     6181 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     6241 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     6301 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     6361 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     6421 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     6481 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     6541 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     6601 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     6661 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     6721 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     6781 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     6841 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     6901 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     6961 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     7021 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     7081 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     7141 ggccttttgc tggccttttg ctcacatgt
//
