LOCUS       Exported                6626 bp ds-DNA     circular SYN 25-NOV-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6626)
  AUTHORS   Charu
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Dec 2, 2019 from SnapGene Viewer 5.0.6
            https://www.snapgene.com
COMMENT     ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..6626
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..141
                     /label=L-ITR
                     /note="/vntifkey=21"
     promoter        156..1419
                     /label=EF-1a
                     /note="/vntifkey=29"
     misc_feature    1426..1459
                     /label=lox2722
                     /note="/vntifkey=21"
     misc_feature    1510..1543
                     /label=loxN
                     /note="/vntifkey=21"
     misc_feature    1550..1563
                     /label=CE donor
                     /label=CE\donor
                     /note="/vntifkey=21"
     misc_feature    complement(1564..1905)
                     /label=mCherry Exon 1
                     /label=Mcherry\Exon1
                     /note="/vntifkey=21"
     misc_feature    1906..1911
                     /label=kozak
     misc_feature    complement(1918..1951)
                     /label=lox 2722 Rev
                     /label=lox\2722\Rev
                     /note="/vntifkey=21"
     misc_feature    complement(2002..2035)
                     /label=lox N Rev
                     /label=lox\N\Rev
                     /note="/vntifkey=21"
     misc_feature    2042..2075
                     /label=FRT
                     /note="/vntifkey=21"
     misc_feature    2126..2159
                     /label=F5
                     /note="/vntifkey=21"
     misc_feature    2168..2235
                     /label=CE bp & acceptor
                     /label=CE\bp\
                     /note="/vntifkey=21"
     misc_feature    2236..2604
                     /label=mCherry Exon 2
                     /label=mCherry\Exon2
                     /note="/vntifkey=21"
     misc_feature    complement(2611..2644)
                     /label=FRT rev
                     /label=FRT\rev
                     /note="/vntifkey=21"
     misc_feature    complement(2695..2728)
                     /label=F5 rev
                     /label=F5\rev
                     /note="/vntifkey=21"
     misc_feature    2743..3351
                     /label=WPRE
                     /note="/vntifkey=21"
     polyA_signal    3371..3849
                     /label=hGH polyA
                     /label=hGH\polyA
                     /note="/vntifkey=25"
     misc_feature    3889..4029
                     /label=R-ITR
                     /note="/vntifkey=21"
     rep_origin      4121..4427
                     /direction=RIGHT
                     /label=f1 Origin
                     /label=f1\Origin
                     /note="/vntifkey=33"
     misc_feature    4946..5803
                     /label=AmpR
                     /note="/vntifkey=21"
     rep_origin      5954..6621
                     /direction=RIGHT
                     /label=pUC ori
                     /label=pUC\ori
                     /note="/vntifkey=33"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaagct ttgcaaagat ggataaagtt
      181 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat
      241 tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
      301 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
      361 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
      421 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
      481 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
      541 tacttccact ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      601 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      661 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      721 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      781 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      841 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      901 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      961 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     1021 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     1081 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     1141 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     1201 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1261 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1321 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1381 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatccataac ttcgtatagg
     1441 atactttata cgaagttatg tagctggagc aatttgaaaa gattgttgta ttacctctag
     1501 ctgcgcttaa taacttcgta tagtatacct tatacgaagt tatgtcgacc ctcctgtact
     1561 cacctgggtc acggtcacca cgccgccgtc ctcgaagttc atcacgcgct cccacttgaa
     1621 gccctcgggg aaggacagct tcaagtagtc ggggatgtcg gcggggtgct tcacgtaggc
     1681 cttggagccg tacatgaact gaggggacag gatgtcccag gcgaagggca gggggccacc
     1741 cttggtcacc ttcagcttgg cggtctgggt gccctcgtag gggcggccct cgccctcgcc
     1801 ctcgatctcg aactcgtggc cgttcacgga gccctccatg tgcaccttga agcgcatgaa
     1861 ctccttgatg atggccatgt tatcctcctc gcccttgctc accatggtgg cactagtata
     1921 acttcgtata aagtatccta tacgaagtta tttgccttaa cccagaaatt atcactgtta
     1981 ttctttagat aggtgcaaag aataacttcg tataaggtat actatacgaa gttatggtac
     2041 cgaagttcct attctctaga aagtatagga acttcgcaga atggtagctg gattgtagct
     2101 gctattagca atatgaaacc tcttagaagt tcctattctt caaaaggtat aggaacttcg
     2161 gcgcgcctgg agagtggcca gcccttctca tgttcagaga acatggttaa ctggttaagt
     2221 catgtcgtcc cacaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
     2281 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     2341 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
     2401 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
     2461 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
     2521 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
     2581 ggcatggacg agctgtacaa gtaagctagc gaagttccta tactttctag agaataggaa
     2641 cttctaagca gacaatcttg taatacatca gcagctagta gattgctcca gctagaagtt
     2701 cctatacctt ttgaagaata ggaacttcga attcgatatc aagcttatcg ataatcaacc
     2761 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     2821 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     2881 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     2941 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     3001 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     3061 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     3121 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctatgt
     3181 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     3241 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     3301 ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc gagcgctgct
     3361 cgagagatct acgggtggca tccctgtgac ccctccccag tgcctctcct ggccctggaa
     3421 gttgccactc cagtgcccac cagccttgtc ctaataaaat taagttgcat cattttgtct
     3481 gactaggtgt ccttctataa tattatgggg tggagggggg tggtatggag caaggggcaa
     3541 gttgggaaga caacctgtag ggcctgcggg gtctattggg aaccaagctg gagtgcagtg
     3601 gcacaatctt ggctcactgc aatctccgcc tcctgggttc aagcgattct cctgcctcag
     3661 cctcccgagt tgttgggatt ccaggcatgc atgaccaggc tcagctaatt tttgtttttt
     3721 tggtagagac ggggtttcac catattggcc aggctggtct ccaactccta atctcaggtg
     3781 atctacccac cttggcctcc caaattgctg ggattacagg cgtgaaccac tgctcccttc
     3841 cctgtccttc tgattttgta ggtaaccacg tgcggaccga gcggccgcag gaacccctag
     3901 tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa
     3961 aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct
     4021 gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     4081 gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     4141 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     4201 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     4261 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt
     4321 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     4381 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     4441 ctcgggctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     4501 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt
     4561 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
     4621 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     4681 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     4741 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     4801 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     4861 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     4921 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     4981 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     5041 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     5101 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     5161 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     5221 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     5281 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     5341 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     5401 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     5461 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     5521 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     5581 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     5641 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     5701 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     5761 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     5821 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     5881 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     5941 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     6001 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     6061 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     6121 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     6181 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     6241 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     6301 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     6361 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     6421 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     6481 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     6541 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     6601 cttttgctgg ccttttgctc acatgt
//
