LOCUS       pAAV-nEF-Coff/Fon\hChR2(E123T-T159C)-EYFP-WPRE        6977 bp    DNA     circular     28-NOV-2016
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|-11506250|
COMMENT     VNTDBDATE|-11521941|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAAV-nEF-Coff/Fon hChR2(E123T-T159C)-EYFP-WPRE|
COMMENT     VNTAUTHORNAME|Charu|
COMMENT     VNTAUTHOREML|charur@stanford.edu|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pAAV-nEF-Coff/Fon hChR2(E123T-T159C)-EYFP-WPRE" 0 6977 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 1 "MAIN" 1 1 1 1 0 0 1 1 1 0 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) 
COMMENT        (CWordArray 47 49 50 48 51 52 81 0 1 3 4 82 5 83 2 6 7 9 61 102 10 11 12 
COMMENT         13 14 15 16 17 18 19 84 20 85 21 22 86 87 88 23 54 62 24 89 103 104 274 
COMMENT         25 26 55 91 27 28 29 30 31 92 32 34 35 33 53 58 93 56 94 37 105 57 38 
COMMENT         36 39 40 41 42 43 95 44 59 99 96 97 45 100 46) (CWordArray) 
COMMENT        (CStringList "ApaLI" "AvaI" "BamHI" "ClaI" "EcoRI" "HindIII" "NcoI" 
COMMENT         "PstI" "SmaI" "XmaI" "AarI" "AatII" "Acc65I" "AccI" "AceIII" "AciI" 
COMMENT         "AclI" "AcuI" "AfeI" "AflII" "AflIII" "AgeI" "AhdI" "AleI" "AloI" 
COMMENT         "AluI" "AlwI" "AlwNI" "ApaBI" "ApaI" "ApoI" "AscI" "AseI" "AsiSI" 
COMMENT         "AvaII" "AvrII" "BaeI" "BanI" "BanII" "BbeI" "Bbr7I" "BbsI" "BbvCI" 
COMMENT         "BbvI" "BccI" "BceAI" "BcefI" "BcgI" "BciVI" "BclI" "BfaI" "BfrBI" 
COMMENT         "BglI" "BglII" "BlpI" "Bme1580I" "BmgBI" "BmrI" "BmtI" "BplI" "BpmI" 
COMMENT         "Bpu10I" "BpuEI" "BsaAI" "BsaBI" "BsaHI" "BsaI" "BsaJI" "BsaWI" "BsaXI" 
COMMENT         "BscAI" "BseMII" "BseRI" "BseYI" "BsgI" "BsiEI" "BsiHKAI" "BsiWI" 
COMMENT         "BslI" "BsmAI" "BsmBI" "BsmFI" "BsmI" "Bsp1286I" "Bsp24I" "BspCNI" 
COMMENT         "BspEI" "BspHI" "BspMI" "BsrBI" "BsrDI" "BsrFI" "BsrGI" "BsrI" "BssHII" 
COMMENT         "BssSI" "BstAPI" "BstBI" "BstEII" "BstF5I" "BstKTI" "BstNI" "BstUI" 
COMMENT         "BstXI" "BstYI" "BstZ17I" "Bsu36I" "BtgI" "BthCI" "BtsI" "Cac8I" "CdiI" 
COMMENT         "ChaI" "CjeI" "CjePI" "Csp6I" "CviAII" "CviJI" "DdeI" "DpnI" "DraI" 
COMMENT         "DraIII" "DrdI" "EaeI" "EagI" "EarI" "EciI" "Eco57MI" "EcoHI" "EcoICRI" 
COMMENT         "EcoNI" "EcoO109I" "EcoRV" "EsaBC3I" "FalI" "FatI" "FauI" "FmuI" 
COMMENT         "Fnu4HI" "FokI" "FseI" "FspAI" "FspI" "GdiII" "HaeI" "HaeII" "HaeIII" 
COMMENT         "HaeIV" "HgaI" "HhaI" "Hin4I" "HincII" "HinfI" "HinP1I" "HpaI" "HpaII" 
COMMENT         "HphI" "Hpy188I" "Hpy188III" "Hpy8I" "Hpy99I" "HpyAV" "HpyCH4I" 
COMMENT         "HpyCH4III" "HpyCH4IV" "HpyCH4V" "HpyF10VI" "KasI" "KpnI" "LpnI" 
COMMENT         "MaeIII" "MboI" "MboII" "MfeI" "MluI" "MlyI" "MmeI" "MnlI" "MscI" 
COMMENT         "MseI" "MslI" "MspA1I" "MwoI" "NaeI" "NarI" "NciI" "NdeI" "NgoMIV" 
COMMENT         "NheI" "NlaIII" "NlaIV" "Nli3877I" "NotI" "NruI" "NsiI" "NspI" "PacI" 
COMMENT         "PciI" "PflMI" "PfoI" "PleI" "PmeI" "PmlI" "PpiI" "Ppu10I" "PpuMI" 
COMMENT         "PshAI" "PsiI" "Psp03I" "PspGI" "PspOMI" "PsrI" "PssI" "PvuI" "PvuII" 
COMMENT         "RleAI" "RsaI" "RsrII" "SacI" "SacII" "SalI" "SanDI" "SapI" "Sau96I" 
COMMENT         "SbfI" "ScaI" "SciI" "ScrFI" "SelI" "SexAI" "SfaNI" "SfcI" "SfiI" 
COMMENT         "SfoI" "SgrAI" "SimI" "SmlI" "SnaBI" "SpeI" "SphI" "SrfI" "Sse232I" 
COMMENT         "Sse8647I" "SspD5I" "SspI" "Sth132I" "StsI" "StuI" "StyD4I" "StyI" 
COMMENT         "SwaI" "TaiI" "TaqI" "TaqII" "TatI" "TauI" "TfiI" "TseI" "Tsp45I" 
COMMENT         "Tsp509I" "TspDTI" "TspGWI" "TspRI" "Tth111I" "Tth111II" "UnbI" "UthSI" 
COMMENT         "VpaK11AI" "XbaI" "XcmI" "XhoI" "XmnI" "ZraI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 1 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 6977 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 4 3314 0 4916 0 5413 0 
COMMENT            6659 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 10 41 0 52 0 215 0 317 0 
COMMENT            2660 0 3711 0 4016 0 4326 0 4337 0 4793 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 703 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 2 3100 0 3695 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 3081 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 2 404 0 3093 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 4 1763 0 2204 0 2258 0 2947 
COMMENT            0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 4 7 0 1349 0 2787 0 4379 0 
COMMENT            0 "") 
COMMENT        #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 4 43 0 54 0 4328 0 4339 0 0 
COMMENT            "") 
COMMENT        #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 4 41 0 52 0 4326 0 4337 0 0 
COMMENT            "") #10=(CRSite (CStringList) "AarI" "cacctgc" 12 16 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "AatII" "gacgtc" 6 0 2 3558 0 5166 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "Acc65I" "ggtacc" 2 0 1 2388 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "AccI" "gtmkac" 3 0 3 1465 0 1568 0 2494 0 0 
COMMENT             "") 
COMMENT        #14=(CRSite (CStringList) "AceIII" "cagctc" 14 18 10 856 0 1006 1 1189 0 
COMMENT             1722 0 1755 0 2053 0 3381 0 4842 1 5039 1 5781 1 0 "") 
COMMENT        #15=(CRSite (CStringList) "AciI" "ccgc" 2 4 77 38 0 143 1 147 0 270 1 
COMMENT             307 0 387 0 439 0 442 0 457 0 488 0 495 0 522 0 872 1 875 1 935 1 
COMMENT             1048 1 1186 1 1225 1 1292 1 1333 1 1771 1 1775 0 1837 0 1863 1 2082 
COMMENT             1 2131 1 2712 0 2799 1 2820 0 2937 0 3414 1 3427 0 3440 0 3550 1 
COMMENT             3591 1 3605 0 3607 1 3626 1 3636 0 3682 0 3690 0 3918 1 3978 0 4224 
COMMENT             1 4233 1 4237 0 4342 1 4392 1 4418 1 4431 0 4468 1 4482 1 4485 1 
COMMENT             4513 0 4540 0 4945 0 4971 0 4981 0 5020 0 5194 1 5241 0 5340 1 5449 
COMMENT             1 5526 1 5570 0 5691 1 5737 0 5928 1 6019 1 6381 0 6390 1 6525 0 
COMMENT             6635 1 6756 1 6775 1 6902 1 6930 1 0 "") 
COMMENT        #16=(CRSite (CStringList) "AclI" "aacgtt" 3 0 4 363 0 4899 0 5483 0 5856 
COMMENT             0 0 "") 
COMMENT        #17=(CRSite (CStringList) "AcuI" "ctgaag" 23 21 5 422 0 1159 1 1614 1 
COMMENT             5419 0 6431 1 0 "") 
COMMENT        #18=(CRSite (CStringList) "AfeI" "agcgct" 4 0 1 3706 0 0 "") 
COMMENT        #19=(CRSite (CStringList) "AflII" "cttaag" 2 0 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "AflIII" "acrygt" 2 0 2 151 0 6973 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "AgeI" "accggt" 2 0 1 2531 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "AhdI" "gacnnnnngtc" 7 0 5 71 0 2252 0 3838 0 
COMMENT             4312 0 6085 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "AleI" "cacnnnngtg" 6 0 5 1196 0 1373 0 1735 0 
COMMENT             1765 0 3782 0 0 "") 
COMMENT        #24=(CRSite (CStringList) "AloI" "gaacnnnnnntcc" 26 21 3 695 1 4200 0 
COMMENT             4725 1 0 "") 
COMMENT        #25=(CRSite (CStringList) "AluI" "agct" 3 0 36 12 0 400 0 406 0 544 0 
COMMENT             685 0 754 0 765 0 846 0 946 0 1020 0 1131 0 1179 0 1395 0 1515 0 
COMMENT             1526 0 1640 0 1712 0 1745 0 2043 0 2959 0 3095 0 3371 0 3939 0 4056 
COMMENT             0 4370 0 4599 0 4856 0 5034 0 5053 0 5732 0 5795 0 5895 0 6416 0 
COMMENT             6673 0 6719 0 6809 0 0 "") 
COMMENT        #26=(CRSite (CStringList) "AlwI" "ggatc" 10 11 17 167 0 672 1 698 1 711 
COMMENT             0 859 1 1058 0 1903 0 2848 1 3675 0 5446 0 5450 1 5767 0 6230 1 
COMMENT             6231 0 6327 1 6329 0 6415 0 0 "") 
COMMENT        #27=(CRSite (CStringList) "AlwNI" "cagnnnctg" 7 0 2 2599 0 6564 0 0 "") 
COMMENT        #28=(CRSite (CStringList) "ApaBI" "gcannnnntgc" 9 0 1 2600 0 0 "") 
COMMENT        #29=(CRSite (CStringList) "ApaI" "gggccc" 6 0 1 2011 0 0 "") 
COMMENT        #30=(CRSite (CStringList) "ApoI" "raatty" 2 0 4 3081 0 3124 0 4869 0 
COMMENT             4880 0 0 "") 
COMMENT        #31=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 1 828 0 0 "") 
COMMENT        #32=(CRSite (CStringList) "AseI" "attaat" 3 0 2 2354 0 5910 0 0 "") 
COMMENT        #33=(CRSite (CStringList) "AsiSI" "gcgatcgc" 6 0 1 169 0 0 "") 
COMMENT        #34=(CRSite (CStringList) "AvaII" "ggwcc" 2 0 7 894 0 2863 0 2873 0 3593 
COMMENT             0 4226 0 5721 0 5943 0 0 "") 
COMMENT        #35=(CRSite (CStringList) "AvrII" "cctagg" 2 0 0 0 "") 
COMMENT        #36=(CRSite (CStringList) "BaeI" "acnnnngtayc" 24 19 0 0 "") 
COMMENT        #37=(CRSite (CStringList) "BanI" "ggyrcc" 2 0 10 176 0 251 0 503 0 694 0 
COMMENT             1726 0 2084 0 2388 0 4382 0 4645 0 6132 0 0 "") 
COMMENT        #38=(CRSite (CStringList) "BanII" "grgcyc" 6 0 5 418 0 2011 0 2961 0 
COMMENT             3458 0 4615 0 0 "") 
COMMENT        #39=(CRSite (CStringList) "BbeI" "ggcgcc" 6 0 2 698 0 4386 0 0 "") 
COMMENT        #40=(CRSite (CStringList) "Bbr7I" "gaagac" 14 18 3 1791 1 3628 1 3910 0 
COMMENT             0 "") 
COMMENT        #41=(CRSite (CStringList) "BbsI" "gaagac" 9 13 3 1796 1 3633 1 3905 0 0 
COMMENT             "") #42=(CRSite (CStringList) "BbvCI" "cctcagc" 3 6 1 4008 0 0 "") 
COMMENT        #43=(CRSite (CStringList) "BbvI" "gcagc" 14 18 29 21 0 752 1 978 0 981 0 
COMMENT             1007 1 1014 1 1298 1 1404 0 1513 1 2587 0 2605 0 2759 1 2764 0 3173 
COMMENT             1 3429 1 3505 1 3601 1 3694 1 4357 1 4379 0 4516 0 5040 1 5651 1 
COMMENT             6042 0 6345 1 6551 1 6554 1 6644 0 6976 1 0 "") 
COMMENT        #44=(CRSite (CStringList) "BccI" "ccatc" 10 11 12 133 0 468 0 982 1 1252 
COMMENT             1 1716 1 2842 0 4248 1 4671 1 4703 0 5628 1 5915 1 6039 1 0 "") 
COMMENT        #45=(CRSite (CStringList) "BceAI" "acggc" 18 20 18 341 1 509 1 654 1 964 
COMMENT             1 1015 1 1057 1 1135 1 1225 1 1336 1 1629 1 1677 1 1689 1 1799 0 
COMMENT             2795 1 2938 0 3427 0 4659 0 6473 1 0 "") 
COMMENT        #46=(CRSite (CStringList) "BcefI" "acggc" 18 19 18 342 1 510 1 655 1 965 
COMMENT             1 1016 1 1058 1 1136 1 1226 1 1337 1 1630 1 1678 1 1690 1 1799 0 
COMMENT             2796 1 2938 0 3427 0 4659 0 6474 1 0 "") 
COMMENT        #47=(CRSite (CStringList) "BcgI" "cgannnnnntgc" 25 23 3 1643 1 4019 0 
COMMENT             5547 1 0 "") 
COMMENT        #48=(CRSite (CStringList) "BciVI" "gtatcc" 13 12 5 1477 1 2420 0 3174 1 
COMMENT             5248 0 6775 0 0 "") 
COMMENT        #49=(CRSite (CStringList) "BclI" "tgatca" 2 0 0 0 "") 
COMMENT        #50=(CRSite (CStringList) "BfaI" "ctag" 2 0 12 131 0 256 0 529 0 591 0 
COMMENT             1589 0 2513 0 3835 0 4249 0 4533 0 5892 0 6227 0 6480 0 0 "") 
COMMENT        #51=(CRSite (CStringList) "BfrBI" "atgcat" 4 0 1 4041 0 0 "") 
COMMENT        #52=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 3 43 0 4342 0 5967 0 
COMMENT             0 "") 
COMMENT        #53=(CRSite (CStringList) "BglII" "agatct" 2 0 2 2698 0 3717 0 0 "") 
COMMENT        #54=(CRSite (CStringList) "BlpI" "gctnagc" 3 0 1 4052 0 0 "") 
COMMENT        #55=(CRSite (CStringList) "Bme1580I" "gkgcmc" 6 0 12 181 0 1139 0 1388 0 
COMMENT             1729 0 2011 0 2385 0 3318 0 3471 0 3789 0 4920 0 5417 0 6663 0 0 "") 
COMMENT        #56=(CRSite (CStringList) "BmgBI" "cacgtc" 4 4 0 0 "") 
COMMENT        #57=(CRSite (CStringList) "BmrI" "actggg" 12 11 3 289 0 3743 1 6045 0 0 
COMMENT             "") #58=(CRSite (CStringList) "BmtI" "gctagc" 6 0 1 2516 0 0 "") 
COMMENT        #59=(CRSite (CStringList) "BplI" "gagnnnnnctc" 25 0 1 3726 0 0 "") 
COMMENT        #60=(CRSite (CStringList) "BpmI" "ctggag" 23 21 6 871 1 1111 1 1444 1 
COMMENT             3765 1 3961 0 6016 0 0 "") 
COMMENT        #61=(CRSite (CStringList) "Bpu10I" "cctnagc" 3 6 7 545 1 929 1 947 1 
COMMENT             1987 1 2284 1 2544 1 4008 0 0 "") 
COMMENT        #62=(CRSite (CStringList) "BpuEI" "cttgag" 23 21 4 5481 0 6349 0 6590 1 
COMMENT             6888 0 0 "") 
COMMENT        #63=(CRSite (CStringList) "BsaAI" "yacgtr" 4 0 2 4221 0 4686 0 0 "") 
COMMENT        #64=(CRSite (CStringList) "BsaBI" "gatnnnnatc" 6 0 0 0 "") 
COMMENT        #65=(CRSite (CStringList) "BsaHI" "grcgyc" 3 0 8 57 0 695 0 1993 0 3555 
COMMENT             0 4323 0 4383 0 5163 0 5545 0 0 "") 
COMMENT        #66=(CRSite (CStringList) "BsaI" "ggtctc" 8 12 3 547 1 4114 0 6019 0 0 
COMMENT             "") 
COMMENT        #67=(CRSite (CStringList) "BsaJI" "ccnngg" 2 0 24 41 0 52 0 318 0 578 0 
COMMENT             1139 0 1194 0 1218 0 1381 0 1733 0 1763 0 1793 0 2204 0 2258 0 2947 
COMMENT             0 3409 0 3481 0 3513 0 3605 0 3765 0 3984 0 4142 0 4326 0 4337 0 
COMMENT             6813 0 0 "") 
COMMENT        #68=(CRSite (CStringList) "BsaWI" "wccggw" 2 0 5 173 0 2531 0 5789 0 
COMMENT             6620 0 6767 0 0 "") 
COMMENT        #69=(CRSite (CStringList) "BsaXI" "acnnnnnctcc" 22 19 3 1608 0 4198 0 
COMMENT             4725 1 0 "") 
COMMENT        #70=(CRSite (CStringList) "BscAI" "gcatc" 10 12 16 428 0 1062 1 1161 1 
COMMENT             1176 1 1662 0 3700 0 3738 0 3827 0 4382 1 4418 0 4934 1 5024 0 5387 
COMMENT             1 5632 0 5827 1 6879 1 0 "") 
COMMENT        #71=(CRSite (CStringList) "BseMII" "ctcag" 16 14 24 23 1 98 0 442 1 559 
COMMENT             0 610 0 943 0 961 0 2001 0 2234 1 2298 0 2558 0 2795 1 2872 0 3669 
COMMENT             0 4022 0 4066 0 4139 0 4286 1 4361 0 4937 0 5598 0 6115 1 6281 1 
COMMENT             6690 1 0 "") 
COMMENT        #72=(CRSite (CStringList) "BseRI" "gaggag" 17 15 4 1737 1 2693 1 3230 1 
COMMENT             3291 0 0 "") 
COMMENT        #73=(CRSite (CStringList) "BseYI" "cccagc" 2 6 4 2039 0 2707 0 4159 1 
COMMENT             6669 0 0 "") 
COMMENT        #74=(CRSite (CStringList) "BsgI" "gtgcag" 23 21 5 366 0 1006 1 1639 0 
COMMENT             2585 1 3966 0 0 "") 
COMMENT        #75=(CRSite (CStringList) "BsiEI" "cgrycg" 5 0 7 147 0 169 0 1775 0 4237 
COMMENT             0 5567 0 5716 0 6639 0 0 "") 
COMMENT        #76=(CRSite (CStringList) "BsiHKAI" "gwgcwc" 6 0 7 1891 0 2961 0 3318 0 
COMMENT             4920 0 5417 0 5502 0 6663 0 0 "") 
COMMENT        #77=(CRSite (CStringList) "BsiWI" "cgtacg" 2 0 0 0 "") 
COMMENT        #78=(CRSite (CStringList) "BslI" "ccnnnnnnngg" 8 0 31 59 0 131 0 186 0 
COMMENT             187 0 261 0 323 0 324 0 452 0 500 0 568 0 874 0 1224 0 1387 0 3295 
COMMENT             0 3346 0 3445 0 3514 0 3606 0 3617 0 3766 0 3984 0 4254 0 4326 0 
COMMENT             4467 0 4793 0 4794 0 5047 0 6495 0 6774 0 6940 0 6958 0 0 "") 
COMMENT        #79=(CRSite (CStringList) "BsmAI" "gtctc" 7 11 10 547 1 1821 0 2239 1 
COMMENT             3271 0 4072 1 4114 0 5048 0 5090 1 5243 1 6019 0 0 "") 
COMMENT        #80=(CRSite (CStringList) "BsmBI" "cgtctc" 8 12 3 4072 1 5048 0 5090 1 0 
COMMENT             "") 
COMMENT        #81=(CRSite (CStringList) "BsmFI" "gggac" 16 20 8 197 1 1414 0 1901 1 
COMMENT             2240 1 2876 0 3395 0 3555 1 3566 0 0 "") 
COMMENT        #82=(CRSite (CStringList) "BsmI" "gaatgc" 8 6 0 0 "") 
COMMENT        #83=(CRSite (CStringList) "Bsp1286I" "gdgchc" 6 0 18 181 0 418 0 1139 0 
COMMENT             1388 0 1729 0 1891 0 2011 0 2385 0 2961 0 3318 0 3458 0 3471 0 3789 
COMMENT             0 4615 0 4920 0 5417 0 5502 0 6663 0 0 "") 
COMMENT        #84=(CRSite (CStringList) "Bsp24I" "gacnnnnnntgg" 25 20 5 1987 0 3350 1 
COMMENT             5185 0 6279 1 6457 1 0 "") 
COMMENT        #85=(CRSite (CStringList) "BspCNI" "ctcag" 15 13 24 24 1 97 0 443 1 558 
COMMENT             0 609 0 942 0 960 0 2000 0 2235 1 2297 0 2557 0 2796 1 2871 0 3668 
COMMENT             0 4021 0 4065 0 4138 0 4287 1 4360 0 4936 0 5597 0 6116 1 6282 1 
COMMENT             6691 1 0 "") #86=(CRSite (CStringList) "BspEI" "tccgga" 2 0 0 0 "") 
COMMENT        #87=(CRSite (CStringList) "BspHI" "tcatga" 2 0 3 5140 0 5245 0 6253 0 0 
COMMENT             "") #88=(CRSite (CStringList) "BspMI" "acctgc" 11 15 1 1898 1 0 "") 
COMMENT        #89=(CRSite (CStringList) "BsrBI" "ccgctc" 4 4 3 4233 1 4542 0 5243 0 0 
COMMENT             "") 
COMMENT        #90=(CRSite (CStringList) "BsrDI" "gcaatg" 9 7 3 3352 1 5851 0 6025 1 0 
COMMENT             "") 
COMMENT        #91=(CRSite (CStringList) "BsrFI" "rccggy" 2 0 10 468 0 600 0 691 0 1611 
COMMENT             0 2067 0 2196 0 2531 0 3617 0 4581 0 6000 0 0 "") 
COMMENT        #92=(CRSite (CStringList) "BsrGI" "tgtaca" 2 0 1 840 0 0 "") 
COMMENT        #93=(CRSite (CStringList) "BsrI" "actgg" 7 5 18 284 0 305 0 944 0 1417 1 
COMMENT             1461 1 3146 0 3347 0 3749 1 3782 1 4775 0 5440 0 5610 1 5879 0 5922 
COMMENT             0 6040 0 6446 0 6558 1 6571 1 0 "") 
COMMENT        #94=(CRSite (CStringList) "BssHII" "gcgcgc" 2 0 5 15 0 100 0 828 0 4278 
COMMENT             0 4363 0 0 "") 
COMMENT        #95=(CRSite (CStringList) "BssSI" "cacgag" 2 6 5 1369 0 1890 1 5109 1 
COMMENT             5416 0 6800 0 0 "") 
COMMENT        #96=(CRSite (CStringList) "BstAPI" "gcannnnntgc" 8 0 1 2599 0 0 "") 
COMMENT        #97=(CRSite (CStringList) "BstBI" "ttcgaa" 3 0 1 3079 0 0 "") 
COMMENT        #98=(CRSite (CStringList) "BstEII" "ggtnacc" 2 0 1 4213 0 0 "") 
COMMENT        #99=(CRSite (CStringList) "BstF5I" "ggatg" 8 6 10 460 1 1149 0 1727 0 
COMMENT             2563 0 2834 1 3730 1 5016 1 5639 0 5926 0 6107 0 0 "") 
COMMENT        #100=(CRSite (CStringList) "BstKTI" "gatc" 4 0 28 162 0 169 0 680 0 706 
COMMENT              0 867 0 905 0 1053 0 1829 0 1898 0 1951 0 2701 0 2856 0 3670 0 
COMMENT              3720 0 4134 0 5405 0 5441 0 5458 0 5716 0 5762 0 5780 0 6121 0 
COMMENT              6226 0 6238 0 6316 0 6324 0 6335 0 6410 0 0 "") 
COMMENT        #101=(CRSite (CStringList) "BstNI" "ccwgg" 3 0 20 1141 0 1195 0 1270 0 
COMMENT              1382 0 1719 0 2079 0 2580 0 2727 0 2867 0 3257 0 3539 0 3761 0 
COMMENT              3767 0 3985 0 4034 0 4048 0 4102 0 6814 0 6827 0 6948 0 0 "") 
COMMENT        #102=(CRSite (CStringList) "BstUI" "cgcg" 3 0 31 17 0 102 0 153 0 270 0 
COMMENT              435 0 480 0 830 0 907 0 1225 0 1905 0 2152 0 2799 0 2822 0 2915 0 
COMMENT              3550 0 3607 0 3638 0 4280 0 4365 0 4458 0 4482 0 4502 0 4878 0 
COMMENT              4989 0 5092 0 5094 0 5194 0 5526 0 6019 0 6349 0 6930 0 0 "") 
COMMENT        #103=(CRSite (CStringList) "BstXI" "ccannnnnntgg" 9 0 3 2211 0 2684 0 
COMMENT              4159 0 0 "") 
COMMENT        #104=(CRSite (CStringList) "BstYI" "rgatcy" 2 0 13 159 0 677 0 703 0 
COMMENT              1050 0 2698 0 3667 0 3717 0 5438 0 5455 0 6223 0 6235 0 6321 0 
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COMMENT                             "CCANNNNNNGTNNNNNNNNNNNNNNNN" 
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COMMENT                             "CCANNNNNNNTCNNNNNNNNNNNNNNN" 
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COMMENT                              (CGroupPar (CParagraph 68169936 (8 0) 1 1 1 0 178) 
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COMMENT                            (CObjectList 
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COMMENT                            (CObjectList 
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FEATURES             Location/Qualifiers
     LTR             1..141
                     /vntifkey="19"
                     /label=L-ITR
     misc_feature    3094..3702
                     /vntifkey="21"
                     /label=WPRE
     polyA_signal    3722..4200
                     /vntifkey="25"
                     /label=hGH\polyA
     LTR             4240..4380
                     /vntifkey="19"
                     /label=R-ITR
     rep_origin      4472..4778
                     /vntifkey="33"
                     /label=f1\Origin
     misc_feature    5297..6154
                     /vntifkey="21"
                     /label=AmpR
     rep_origin      6305..6972
                     /vntifkey="33"
                     /label=pUC\ori
     promoter        156..701
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /vntifkey="29"
                     /label=EF1\promoter
     misc_feature    792..825
                     /vntifkey="21"
                     /label=F5
     misc_feature    708..741
                     /vntifkey="21"
                     /label=F3
     misc_feature    complement(2962..2995)
                     /vntifkey="21"
                     /label=F3\Rev
     misc_feature    complement(3046..3079)
                     /vntifkey="21"
                     /label=F5\Rev
     misc_feature    1469..1502
                     /vntifkey="21"
                     /label=lox2722
     misc_feature    1553..1586
                     /vntifkey="21"
                     /label=loxN
     misc_feature    complement(2477..2510)
                     /vntifkey="21"
                     /label=loxN\Rev
     misc_feature    complement(2393..2426)
                     /vntifkey="21"
                     /label=lox2722\Rev
     misc_feature    complement(834..1394)
                     /vntifkey="21"
                     /label=YFP\exon\3\with\STOP
     misc_feature    complement(1395..1402)
                     /vntifkey="21"
                     /label=CE\intron\acceptor
     misc_feature    complement(1404..1462)
                     /vntifkey="21"
                     /label=CE\intron\BP
     misc_feature    complement(2517..2531)
                     /vntifkey="21"
                     /label=CMV\intron\donor
     misc_feature    complement(2581..2583)
                     /vntifkey="21"
                     /label=E123T
     misc_feature    complement(2532..2949)
                     /vntifkey="21"
                     /label=ChR2\tc\Ex1
     misc_feature    complement(1778..2289)
                     /vntifkey="21"
                     /label=ChR2\tc\Ex2
     misc_feature    complement(2231..2233)
                     /vntifkey="21"
                     /label=T159C
     misc_feature    complement(1769..1777)
                     /vntifkey="21"
                     /label=Spacer
     misc_feature    complement(2306..2386)
                     /vntifkey="21"
                     /label=CMV\intron\branch\point
     misc_feature    complement(2290..2305)
                     /vntifkey="21"
                     /label=CMV\intron\acceptor
     misc_feature    complement(1593..1606)
                     /vntifkey="21"
                     /label=CE\intron\donor
     misc_feature    complement(1607..1762)
                     /vntifkey="21"
                     /label=YFP\START
BASE COUNT     1535 a      1864 c      1818 g      1760 t 
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 
      121 actccatcac taggggttcc tgcggccgca cgcgtaagga tctgcgatcg ctccggtgcc 
      181 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc 
      241 aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac 
      301 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg 
      361 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc 
      421 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc 
      481 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta 
      541 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc 
      601 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct 
      661 gttctgcgcc gttacagatc caagctgtga ccggcgccta cggatccgaa gttcctattc 
      721 ttcaaatagt ataggaactt cgcagaatgg tagctggatt gtagctgcta ttagcaatat 
      781 gaaacctctt agaagttcct attcttcaaa aggtatagga acttcggcgc gccttacttg 
      841 tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag caggaccatg 
      901 tgatcgcgct tctcgttggg gtctttgctc agggcggact ggtagctcag gtagtggttg 
      961 tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc 
     1021 tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt tcaccttgat gccgttcttc 
     1081 tgcttgtcgg ccatgatata gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc 
     1141 aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt caccagggtg 
     1201 tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg 
     1261 gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg 
     1321 tggtcggggt agcgggcgaa gcactgcagg ccgtagccga aggtggtcac gagggtgggc 
     1381 cagggcacgg gcagctgtgg gacgacatga cttaaccagt taaccatgtt ctctgaacat 
     1441 gagaagggct ggccactctc cagtcgacat aacttcgtat aggatacttt atacgaagtt 
     1501 atgcagaatg gtagctggat tgtagctgct attagcaata tgaaacctct taataacttc 
     1561 gtatagtata ccttatacga agttatacta gtcctcctgt actcaccttg ccggtggtgc 
     1621 agatgaactt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc 
     1681 tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga 
     1741 acagctcctc gcccttgctc accatggtgg cggccgctgg cacggctccg gcctcggctt 
     1801 cgtcttcgac gagagtctcg acctcgatct ccgttccgcc gatgttcagt ttggtggttt 
     1861 tgcggatatc tccgtgaatc aatatgtgct cgtggatcag gacgcgcagg tagtgtccca 
     1921 acaaccccca acaattttta ctcatcagat caataatcgt gtgacctacg gtggagccat 
     1981 agacgctcag gacgccaaaa ccttcgggcc ccaaaatgaa gagaattggg aacatacccc 
     2041 agctcacgaa aaacagccat gccatgccgg tcacgacctg gcggcaccga ccctttggca 
     2101 cagtatgata accctcgata tatgctttgg cggcgtgaaa aaatgtgttc gcgccatagc 
     2161 acaatccaag acaaaagaag atgactttaa catagccggt tgccatggcg ctggtagccc 
     2221 cccacacgat acacccgatg tctgagacaa ggagtcccat ggttctcctg ctgtagtcgt 
     2281 tgctcaggcc taaacacatg agaaatagag tcataagcac atgggtcaca tacaggagat 
     2341 atgtatataa cattaataca attttataaa aaaaaggggg ggcacaggta ccataacttc 
     2401 gtataaagta tcctatacga agttatttgc cttaacccag aaattatcac tgttattctt 
     2461 tagaatggtg caaagaataa cttcgtataa ggtatactat acgaagttat gctagcaacc 
     2521 ccgacactta ccggtgaggt tgctcaggtg gataaggatg acaggacaag tgagcagcca 
     2581 ggttgcatag cgcagccact gcacccggtg tcctgtggca aggtagagca tagagggatt 
     2641 cttaaactca aaaaagaact cgagaatcac cttaaccatt tcaatggcgc acacatagat 
     2701 ctcctcccag ccgcatgtag atttccaggt ttggtaggca tagaacatca gcagcaaaat 
     2761 gctgaatcct gctgcaagcc actgcaggac atttgacgcg gtctgagcgc cgttcgtgcc 
     2821 gcgagattca atccatccgg cacagtaaca ttgatcctca gggaccagga cggacccgtt 
     2881 caccaccaca ggattagtaa cgaacaaaag ttcgcgtccg acggcagaca aagcgccgcc 
     2941 atagtccatg gtggcgagct cgaagttcct atactatttg aagaatagga acttcttgcc 
     3001 ttaacccaga aattatcact gttattcttt agaatggtgc aaagagaagt tcctatacct 
     3061 tttgaagaat aggaacttcg aattcgatat caagcttatc gataatcaac ctctggatta 
     3121 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg 
     3181 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc 
     3241 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca 
     3301 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac 
     3361 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact 
     3421 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc 
     3481 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg 
     3541 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc 
     3601 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac 
     3661 gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgagcgctgc tcgagagatc 
     3721 tacgggtggc atccctgtga cccctcccca gtgcctctcc tggccctgga agttgccact 
     3781 ccagtgccca ccagccttgt cctaataaaa ttaagttgca tcattttgtc tgactaggtg 
     3841 tccttctata atattatggg gtggaggggg gtggtatgga gcaaggggca agttgggaag 
     3901 acaacctgta gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt ggcacaatct 
     3961 tggctcactg caatctccgc ctcctgggtt caagcgattc tcctgcctca gcctcccgag 
     4021 ttgttgggat tccaggcatg catgaccagg ctcagctaat ttttgttttt ttggtagaga 
     4081 cggggtttca ccatattggc caggctggtc tccaactcct aatctcaggt gatctaccca 
     4141 ccttggcctc ccaaattgct gggattacag gcgtgaacca ctgctccctt ccctgtcctt 
     4201 ctgattttgt aggtaaccac gtgcggaccg agcggccgca ggaaccccta gtgatggagt 
     4261 tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc 
     4321 gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg 
     4381 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc 
     4441 aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac 
     4501 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc 
     4561 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt 
     4621 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg 
     4681 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac 
     4741 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta 
     4801 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat 
     4861 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac 
     4921 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc 
     4981 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac 
     5041 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg 
     5101 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta 
     5161 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta 
     5221 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata 
     5281 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc 
     5341 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga 
     5401 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct 
     5461 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg 
     5521 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta 
     5581 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat 
     5641 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt 
     5701 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga 
     5761 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga 
     5821 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga 
     5881 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc 
     5941 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc 
     6001 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg 
     6061 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat 
     6121 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata 
     6181 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct 
     6241 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga 
     6301 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 
     6361 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 
     6421 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct 
     6481 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 
     6541 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 
     6601 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 
     6661 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 
     6721 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 
     6781 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 
     6841 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 
     6901 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 
     6961 gccttttgct cacatgt 
//
