LOCUS       Exported                6961 bp ds-DNA     circular SYN 25-NOV-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6961)
  AUTHORS   Charu
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Dec 3, 2019 from SnapGene Viewer 5.0.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6961
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..141
                     /label=L-ITR
                     /note="/vntifkey=19"
     promoter        156..701
                     /label=EF1 promoter
                     /label=EF1\promoter
                     /note="/ApEinfo_fwdcolor=cyan"
                     /note="/ApEinfo_revcolor=green"
                     /note="/vntifkey=29"
     misc_feature    708..741
                     /label=FRT
                     /note="/vntifkey=21"
     misc_feature    792..825
                     /label=F5
                     /note="/vntifkey=21"
     misc_feature    834..839
                     /label=KOZAK
                     /note="/vntifkey=21"
     misc_feature    840..1257
                     /label=ChR2(H134R) Exon 1
                     /label=ChR2\exon\1
                     /note="/vntifkey=21"
     misc_feature    1239..1241
                     /label=H134R
     misc_feature    1258..1272
                     /label=CMV intron donor
                     /label=CMV\intron\donor
                     /note="/vntifkey=21"
     misc_feature    1279..1312
                     /label=lox N
                     /label=lox\N
                     /note="/vntifkey=21"
     misc_feature    1363..1396
                     /label=lox 2722
                     /label=lox\2722
                     /note="/vntifkey=21"
     misc_feature    1403..1416
                     /label=CE intron donor
                     /label=CE\intron\donor
                     /note="/vntifkey=21"
     misc_feature    complement(1417..1755)
                     /label=mCherry Exon 1
                     /label=Mcherry\Exon1
                     /note="/vntifkey=21"
     misc_feature    1756..1764
                     /label=spacer
                     /note="/vntifkey=21"
     misc_feature    complement(1765..2276)
                     /label=ChR2(H134R) Exon 2
                     /label=ChR2\exon\2
                     /note="/vntifkey=21"
     misc_feature    2277..2292
                     /label=CMV intron acceptor
                     /label=CMV\intron\acceptor
                     /note="/vntifkey=21"
     misc_feature    complement(2293..2372)
                     /label=CMV bp
                     /label=CMV\bp
                     /note="/vntifkey=21"
     misc_feature    complement(2379..2412)
                     /label=lox N Rev
                     /label=lox\N\Rev
                     /note="/vntifkey=21"
     misc_feature    complement(2463..2496)
                     /label=lox 2722 Rev
                     /label=lox\2722\Rev
                     /note="/vntifkey=21"
     misc_feature    2503..2561
                     /label=CE bp
                     /label=CE\bp
                     /note="/vntifkey=21"
     misc_feature    2562..2570
                     /label=CE acceptor
                     /label=CE\acceptor
                     /note="/vntifkey=21"
     misc_feature    2571..2939
                     /label=mCherry Exon2
                     /label=mCherry\Exon2
                     /note="/vntifkey=21"
     misc_feature    complement(2946..2979)
                     /label=FRT rev
                     /label=FRT\rev
                     /note="/vntifkey=21"
     misc_feature    complement(3030..3063)
                     /label=F5 rev
                     /label=F5\rev
                     /note="/vntifkey=21"
     misc_feature    3078..3686
                     /label=WPRE
                     /note="/vntifkey=21"
     polyA_signal    3706..4184
                     /label=hGH polyA
                     /label=hGH\polyA
                     /note="/vntifkey=25"
     LTR             4224..4364
                     /label=R-ITR
                     /note="/vntifkey=19"
     rep_origin      4456..4762
                     /direction=RIGHT
                     /label=f1 Origin
                     /label=f1\Origin
                     /note="/vntifkey=33"
     misc_feature    5281..6138
                     /label=AmpR
                     /note="/vntifkey=21"
     rep_origin      6289..6956
                     /direction=RIGHT
                     /label=pUC ori
                     /label=pUC\ori
                     /note="/vntifkey=33"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaagga tctgcgatcg ctccggtgcc
      181 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
      241 aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
      301 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
      361 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc
      421 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc
      481 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta
      541 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc
      601 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct
      661 gttctgcgcc gttacagatc caagctgtga ccggcgccta cggatccgaa gttcctattc
      721 tctagaaagt ataggaactt cgcagagtag tagctggatt gtagctgcta ttagcaatct
      781 aaaacctctt agaagttcct attcttcaaa aggtatagga acttcggcgc gccgccacca
      841 tggactatgg cggcgctttg tctgccgtcg gacgcgaact tttgttcgtt actaatcctg
      901 tggtggtgaa cgggtccgtc ctggtccctg aggatcaatg ttactgtgcc ggatggattg
      961 aatctcgcgg cacgaacggc gctcagaccg cgtcaaatgt cctgcagtgg cttgcagcag
     1021 gattcagcat tttgctgctg atgttctatg cctaccaaac ctggaaatct acatgcggct
     1081 gggaggagat ctatgtgtgc gccattgaaa tggttaaggt gattctcgag ttcttttttg
     1141 agtttaagaa tccctctatg ctctaccttg ccacaggaca ccgggtgcag tggctgcgct
     1201 atgcagagtg gctgctcact tgtcctgtca tccttatccg cctgagcaac ctcaccggta
     1261 agtgtcgggg ttgctagcat aacttcgtat agtatacctt atacgaagtt attctttgca
     1321 ccattctaaa gaataacagt gataatttct gggttaaggc aaataacttc gtataggata
     1381 ctttatacga agttatggta cccctcctgt actcacctgg gtcacggtca ccacgccgcc
     1441 gtcctcgaag ttcatcacgc gctcccactt gaagccctcg gggaaggaca gcttcaagta
     1501 gtcggggatg tcggcggggt gcttcacgta ggccttggag ccgtacatga actgagggga
     1561 caggatgtcc caggcgaagg gcagggggcc acccttggtc accttcagct tggcggtctg
     1621 ggtgccctcg taggggcggc cctcgccctc gccctcgatc tcgaactcgt ggccgttcac
     1681 ggagccctcc atgtgcacct tgaagcgcat gaactccttg atgatggcca tgttatcctc
     1741 ctcgcccttg ctcacggcgg ccgctggcac ggctccggcc tcggcttcgt cttcgacgag
     1801 agtctcgacc tcgatctccg ttccgccgat gttcagtttg gtggttttgc ggatatctcc
     1861 gtgaatcaat atgtgctcgt ggatcaggac gcgcaggtag tgtcccaaca acccccaaca
     1921 atttttactc atcagatcaa taatcgtgtg acctacggtg gagccataga cgctcaggac
     1981 gccaaaacct tcgggcccca aaatgaagag aattgggaac ataccccagc tcacgaaaaa
     2041 cagccatgcc atgccggtca cgacctggcg gcaccgaccc tttggcacag tatgataacc
     2101 ctcgatatat gctttggcgg cgtgaaaaaa tgtgttcgcg ccatagcaca atccaagaca
     2161 aaagaagatg actttaacat agccggttgc catggcgctg gtagcccccc acacgatagt
     2221 cccgatgtct gagacaagga gtcccatggt tctcctgctg tagtcgttgc tcaggcctaa
     2281 acacatgaga aatagagtca taagcacatg ggtcacatac aggagatatg tatataacat
     2341 taatacaatt tataaaaaaa agggggggca caactagtat aacttcgtat aaggtatact
     2401 atacgaagtt attaagaggt ttcatattgc taatagcagc tacaatccag ctaccattct
     2461 gcataacttc gtataaagta tcctatacga agttatgtcg actggagagt ggccagccct
     2521 tctcatgttc agagaacatg gttaactggt taagtcatgt cgtcccacag gactcctccc
     2581 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
     2641 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg
     2701 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact
     2761 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
     2821 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac
     2881 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaag
     2941 ctagcgaagt tcctatactt tctagagaat aggaacttct aagcagacaa tcttgtaata
     3001 catcagcagc tagtagattg ctccagctag aagttcctat accttttgaa gaataggaac
     3061 ttcgaattcg atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga
     3121 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     3181 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     3241 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     3301 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     3361 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     3421 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     3481 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     3541 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     3601 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     3661 tgggccgcct ccccgcatcg ataccgagcg ctgctcgaga gatctacggg tggcatccct
     3721 gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg cccaccagcc
     3781 ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc tataatatta
     3841 tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc tgtagggcct
     3901 gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc actgcaatct
     3961 ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc cgagttgttg ggattccagg
     4021 catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt ttcaccatat
     4081 tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg cctcccaaat
     4141 tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctgatt ttgtaggtaa
     4201 ccacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct
     4261 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc
     4321 ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta
     4381 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac
     4441 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     4501 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     4561 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     4621 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
     4681 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     4741 ctcttgttcc aaactggaac aacactcaac cctatctcgg gctattcttt tgatttataa
     4801 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     4861 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc
     4921 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     4981 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     5041 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     5101 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     5161 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     5221 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     5281 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     5341 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     5401 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     5461 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     5521 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     5581 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     5641 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     5701 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     5761 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     5821 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     5881 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     5941 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     6001 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     6061 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     6121 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     6181 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     6241 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     6301 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     6361 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     6421 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     6481 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6541 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6601 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6661 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     6721 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6781 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6841 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6901 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6961 t
//
