LOCUS       Exported                6961 bp ds-DNA     circular SYN 25-NOV-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6961)
  AUTHORS   Charu
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Dec 2, 2019 from SnapGene Viewer 5.0.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6961
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..141
                     /label=L-ITR
     promoter        156..701
                     /label=nEF promoter
     misc_feature    708..741
                     /label=F3
     misc_feature    792..825
                     /label=F5
     misc_feature    complement(834..1202)
                     /label=mCherry Exon 3
     misc_feature    1203..1210
                     /label=CE acceptor
     misc_feature    complement(1212..1270)
                     /label=CE bp
     misc_feature    1277..1310
                     /label=lox2722
     misc_feature    1361..1394
                     /label=loxN
     misc_feature    1401..1414
                     /label=CE donor
     misc_feature    complement(1415..1753)
                     /label=mCherry Exon 2
     misc_feature    1754..1762
                     /label=spacer
     misc_feature    complement(1763..2274)
                     /label=ChR2(H134R) Exon 1
     misc_feature    2275..2290
                     /label=CMV acceptor
     misc_feature    complement(2291..2370)
                     /label=CMV bp
     misc_feature    2350..2352
                     /label=SNP TAT -> ATA
     misc_feature    2359..2360
                     /label=SNP AAG -> AG
     misc_feature    complement(2377..2410)
                     /label=lox2722 Rev
     misc_feature    complement(2461..2494)
                     /label=loxN Rev
     misc_feature    2501..2515
                     /label=CMV donor
     misc_feature    complement(2516..2933)
                     /label=ChR2(H134R) Exon 1
     misc_feature    2532..2534
                     /label=H134R
     misc_feature    2934..2939
                     /label=KOZAK
     misc_feature    complement(2946..2979)
                     /label=F3 Rev
     misc_feature    complement(3030..3063)
                     /label=F5 Rev
     misc_feature    3078..3686
                     /label=WPRE
     polyA_signal    3706..4184
                     /label=hGH polyA
     LTR             4224..4364
                     /label=R-ITR
     rep_origin      4456..4762
                     /direction=RIGHT
                     /label=f1 Origin
     misc_feature    5281..6138
                     /label=AmpR
     rep_origin      6289..6956
                     /direction=RIGHT
                     /label=pUC ori
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaagga tctgcgatcg ctccggtgcc
      181 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
      241 aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
      301 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
      361 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc
      421 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc
      481 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta
      541 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc
      601 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct
      661 gttctgcgcc gttacagatc caagctgtga ccggcgccta cggatccgaa gttcctattc
      721 ttcaaatagt ataggaactt cgcagagtag tagctggatt gtagctgcta ttagcaatct
      781 aaaacctctt agaagttcct attcttcaaa aggtatagga acttcggcgc gccttacttg
      841 tacagctcgt ccatgccgcc ggtggagtgg cggccctcgg cgcgttcgta ctgttccacg
      901 atggtgtagt cctcgttgtg ggaggtgatg tccaacttga tgttgacgtt gtaggcgccg
      961 ggcagctgca cgggcttctt ggccttgtag gtggtcttga cctcagcgtc gtagtggccg
     1021 ccgtccttca gcttcagcct ctgcttgatc tcgcccttca gggcgccgtc ctcggggtac
     1081 atccgctcgg aggaggcctc ccagcccatg gtcttcttct gcattacggg gccgtcggag
     1141 gggaagttgg tgccgcgcag cttcaccttg tagatgaact cgccgtcctg cagggaggag
     1201 tcctgtggga cgacatgact taaccagtta accatgttct ctgaacatga gaagggctgg
     1261 ccactctcca gtcgacataa cttcgtatag gatactttat acgaagttat gcagaatggt
     1321 agctggattg tagctgctat tagcaatatg aaacctctta ataacttcgt atagtatacc
     1381 ttatacgaag ttatactagt cctcctgtac tcacctgggt cacggtcacc acgccgccgt
     1441 cctcgaagtt catcacgcgc tcccacttga agccctcggg gaaggacagc ttcaagtagt
     1501 cggggatgtc ggcggggtgc ttcacgtagg ccttggagcc gtacatgaac tgaggggaca
     1561 ggatgtccca ggcgaagggc agggggccac ccttggtcac cttcagcttg gcggtctggg
     1621 tgccctcgta ggggcggccc tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg
     1681 agccctccat gtgcaccttg aagcgcatga actccttgat gatggccatg ttatcctcct
     1741 cgcccttgct cacggcggcc gctggcacgg ctccggcctc ggcttcgtct tcgacgagag
     1801 tctcgacctc gatctccgtt ccgccgatgt tcagtttggt ggttttgcgg atatctccgt
     1861 gaatcaatat gtgctcgtgg atcaggacgc gcaggtagtg tcccaacaac ccccaacaat
     1921 ttttactcat cagatcaata atcgtgtgac ctacggtgga gccatagacg ctcaggacgc
     1981 caaaaccttc gggccccaaa atgaagagaa ttgggaacat accccagctc acgaaaaaca
     2041 gccatgccat gccggtcacg acctggcggc accgaccctt tggcacagta tgataaccct
     2101 cgatatatgc tttggcggcg tgaaaaaatg tgttcgcgcc atagcacaat ccaagacaaa
     2161 agaagatgac tttaacatag ccggttgcca tggcgctggt agccccccac acgatagtcc
     2221 cgatgtctga gacaaggagt cccatggttc tcctgctgta gtcgttgctc aggcctaaac
     2281 acatgagaaa tagagtcata agcacatggg tcacatacag gagatatgta tataacatta
     2341 atacaattta taaaaaaaag ggggggcaca ggtaccataa cttcgtataa agtatcctat
     2401 acgaagttat ttgccttaac ccagaaatta tcactgttat tctttagaat ggtgcaaaga
     2461 ataacttcgt ataaggtata ctatacgaag ttatgctagc aaccccgaca cttaccggtg
     2521 aggttgctca ggcggataag gatgacagga caagtgagca gccactctgc atagcgcagc
     2581 cactgcaccc ggtgtcctgt ggcaaggtag agcatagagg gattcttaaa ctcaaaaaag
     2641 aactcgagaa tcaccttaac catttcaatg gcgcacacat agatctcctc ccagccgcat
     2701 gtagatttcc aggtttggta ggcatagaac atcagcagca aaatgctgaa tcctgctgca
     2761 agccactgca ggacatttga cgcggtctga gcgccgttcg tgccgcgaga ttcaatccat
     2821 ccggcacagt aacattgatc ctcagggacc aggacggacc cgttcaccac cacaggatta
     2881 gtaacgaaca aaagttcgcg tccgacggca gacaaagcgc cgccatagtc catggtggcg
     2941 agctcgaagt tcctatacta tttgaagaat aggaacttct tgccttaacc cagaaattat
     3001 cactgttatt ctttagaatg gtgcaaagag aagttcctat accttttgaa gaataggaac
     3061 ttcgaattcg atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga
     3121 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     3181 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     3241 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     3301 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     3361 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     3421 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     3481 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     3541 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     3601 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     3661 tgggccgcct ccccgcatcg ataccgagcg ctgctcgaga gatctacggg tggcatccct
     3721 gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg cccaccagcc
     3781 ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc tataatatta
     3841 tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc tgtagggcct
     3901 gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc actgcaatct
     3961 ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc cgagttgttg ggattccagg
     4021 catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt ttcaccatat
     4081 tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg cctcccaaat
     4141 tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctgatt ttgtaggtaa
     4201 ccacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct
     4261 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc
     4321 ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta
     4381 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac
     4441 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     4501 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     4561 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     4621 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
     4681 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     4741 ctcttgttcc aaactggaac aacactcaac cctatctcgg gctattcttt tgatttataa
     4801 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     4861 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc
     4921 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     4981 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     5041 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     5101 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     5161 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     5221 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     5281 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     5341 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     5401 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     5461 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     5521 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     5581 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     5641 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     5701 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     5761 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     5821 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     5881 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     5941 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     6001 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     6061 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     6121 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     6181 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     6241 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     6301 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     6361 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     6421 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     6481 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6541 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6601 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6661 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     6721 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6781 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6841 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6901 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6961 t
//
