LOCUS Exported 6360 bp ds-DNA circular SYN 25-NOV-2019 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6360) AUTHORS Charu TITLE Direct Submission JOURNAL Exported Tuesday, Dec 3, 2019 from SnapGene Viewer 5.0.6 https://www.snapgene.com FEATURES Location/Qualifiers source 1..6360 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..141 /label=L-ITR promoter 156..701 /label=nEF promoter misc_feature 708..713 /label=kozak CDS 714..1586 /codon_start=1 /label=NpHR /translation="MTETLPPVTESAVALQAEVTQRELFEFVLNDPLLASSLYINIALA GLSILLFVFMTRGLDDPRAKLIAVSTILVPVVSIASYTGLASGLTISVLEMPAGHFAEG SSVMLGGEEVDGVVTMWGRYLTWALSTPMILLALGLLAGSNATKLFTAITFDIAMCVTG LAAALTTSSHLMRWFFYAISCACFLVVLYILLVEWAQDAKAAGTADMFNTLKLLTVVMW LGYPIVWALGVEGIAVLPVGVTSWGYSFLDIVAKYIFAFLLLNYLTSNESVVSGSILDV PSASGTPADD" misc_feature 714..963 /label=Exon 1 primer_bind 782..801 /label=NpHR exon 1 misc_feature 964..1877 /label=Exon 2 primer_bind complement(1036..1055) /label=NpHR exon 2 misc_feature 1248..1250 /label=W179F misc_feature 1587..1646 /label=TS misc_feature 1656..1664 /label=linker misc_feature 1665..1721 /label=p2A misc_feature 1719 /label=cleavage misc_feature 1722..2462 /label=EYFP misc_feature 1878..2459 /label=Exon 3 primer_bind complement(1950..1969) /label=YFP exon2 R misc_feature 2113 /label=SNP G -> A enhancer 2437..2456 /label=ER export misc_feature 2477..3085 /label=WPRE polyA_signal 3105..3583 /label=hGH polyA LTR 3623..3763 /label=R-ITR rep_origin 3855..4161 /direction=RIGHT /label=f1 Origin misc_feature 4680..5537 /label=AmpR rep_origin 5688..6355 /direction=RIGHT /label=pUC ori ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtaagga tctgcgatcg ctccggtgcc 181 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc 241 aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac 301 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg 361 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc 421 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc 481 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta 541 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc 601 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct 661 gttctgcgcc gttacagatc caagctgtga ccggcgccta cggatccgcc accatgacag 721 agaccctgcc tcccgtgacc gagagtgccg tggcccttca agccgaggtt acccaaaggg 781 agttgttcga gttcgtgctg aacgaccctt tgcttgcaag cagtctctat atcaacatcg 841 cacttgcagg actgagtata ctgctgttcg tttttatgac ccgaggactc gatgatccac 901 gggcaaaact tattgctgtg tcaaccatcc ttgtgcctgt cgtcagcatt gcctcctaca 961 ctggattggc gagcggcctg acaatttccg ttcttgaaat gccagcgggc cattttgcag 1021 aaggcagctc agtgatgctg ggaggagaag aggtagatgg tgtagtcacc atgtggggac 1081 ggtatctcac ctgggcactt tccacgccca tgattctcct cgctctgggt ctcctggccg 1141 gaagcaatgc tacaaagctc ttcacagcta tcactttcga tatcgctatg tgcgtgactg 1201 gccttgccgc ggccctgact acctcctccc acctcatgag atggttcttt tacgctatca 1261 gttgtgcatg ctttctggtg gtcttgtata tcctgctggt ggagtgggca caggacgcca 1321 aagccgcggg aaccgctgac atgttcaata ccctgaagct gttgacagta gtgatgtggc 1381 tggggtatcc aattgtgtgg gctcttggag tcgagggtat cgcggtgttg cccgttgggg 1441 tgacgagctg gggatattct ttcctggata tcgtggcaaa gtacattttc gcattcttgc 1501 tcctgaacta tctgacgtca aacgaatctg tcgtgtccgg cagcattttg gatgttccat 1561 ctgcttctgg gaccccggct gatgataaga gcaggatcac cagcgagggc gagtacatcc 1621 ccctggacca gatcgacatc aacgtgggcg cgcccggctc cggagccacg aacttctctc 1681 tgttaaagca agcaggagac gtggaagaaa accccggtcc cgtgagcaag ggcgaggagc 1741 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt 1801 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca 1861 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttcggctacg 1921 gcctgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg 1981 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca 2041 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg 2101 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca 2161 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga 2221 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc 2281 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac cagtccgccc 2341 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg 2401 ccgggatcac tctcggcatg gacgagctgt acaagttctg ctacgagaac gaggtgtaat 2461 gagaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat 2521 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc 2581 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct 2641 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca 2701 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt 2761 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg 2821 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga 2881 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt 2941 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc 3001 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt 3061 gggccgcctc cccgcatcga taccgagcgc tgctcgagag atctacgggt ggcatccctg 3121 tgacccctcc ccagtgcctc tcctggccct ggaagttgcc actccagtgc ccaccagcct 3181 tgtcctaata aaattaagtt gcatcatttt gtctgactag gtgtccttct ataatattat 3241 ggggtggagg ggggtggtat ggagcaaggg gcaagttggg aagacaacct gtagggcctg 3301 cggggtctat tgggaaccaa gctggagtgc agtggcacaa tcttggctca ctgcaatctc 3361 cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc gagttgttgg gattccaggc 3421 atgcatgacc aggctcagct aatttttgtt tttttggtag agacggggtt tcaccatatt 3481 ggccaggctg gtctccaact cctaatctca ggtgatctac ccaccttggc ctcccaaatt 3541 gctgggatta caggcgtgaa ccactgctcc cttccctgtc cttctgattt tgtaggtaac 3601 cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg 3661 cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc 3721 cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat 3781 tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg 3841 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta 3901 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt 3961 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg 4021 ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat 4081 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac 4141 tcttgttcca aactggaaca acactcaacc ctatctcggg ctattctttt gatttataag 4201 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg 4261 cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct 4321 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac 4381 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca 4441 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac 4501 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt 4561 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt 4621 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta 4681 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg 4741 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac 4801 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg 4861 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc 4921 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg 4981 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat 5041 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg 5101 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg 5161 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc 5221 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt 5281 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct 5341 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc 5401 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca 5461 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct 5521 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt 5581 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga 5641 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca 5701 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac 5761 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg 5821 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag 5881 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac 5941 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt 6001 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg 6061 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc 6121 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc 6181 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc 6241 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa 6301 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt //