LOCUS       Exported                7061 bp ds-DNA     circular SYN 25-NOV-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7061)
  AUTHORS   Charu
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Dec 3, 2019 from SnapGene Viewer 5.0.6
            https://www.snapgene.com
COMMENT     ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..7061
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..141
                     /label=L-ITR
     promoter        156..701
                     /label=nEF promoter
     misc_feature    708..741
                     /label=F3
     misc_feature    792..825
                     /label=F5
     misc_feature    complement(834..1415)
                     /label=EYFP Exon 3
     enhancer        837..857
                     /label=ER export
     misc_feature    1416..1423
                     /label=CE intron acceptor
     misc_feature    complement(1425..1483)
                     /label=CE intron BP
     misc_feature    1490..1523
                     /label=lox2722
     misc_feature    1574..1607
                     /label=loxN
     misc_feature    1614..1694
                     /label=CMV intron branch point
     misc_feature    1695..1710
                     /label=CMV intron acceptor
     misc_feature    1711..2333
                     /label=NpHR Exon 2
     misc_feature    1995..1997
                     /label=W179F
     misc_feature    2334..2393
                     /label=TS
     misc_feature    2403..2411
                     /label=linker
     misc_feature    2412..2468
                     /label=p2A
     misc_feature    2466
                     /label=cleavage
     misc_feature    2469..2624
                     /label=EYFP Exon 2
     misc_feature    2625..2638
                     /label=CE intron donor
     misc_feature    complement(2645..2678)
                     /label=lox2722 Rev
     misc_feature    complement(2729..2762)
                     /label=loxN Rev
     misc_feature    2769..2783
                     /label=CMV intron donor
     misc_feature    complement(2784..3033)
                     /label=NpHR Exon 1
     misc_feature    3034..3039
                     /label=kozak
     misc_feature    complement(3046..3079)
                     /label=F3 Rev
     misc_feature    complement(3130..3163)
                     /label=F5 Rev
     misc_feature    3178..3786
                     /label=WPRE
     polyA_signal    3806..4284
                     /label=hGH polyA
     misc_feature    4324..4464
                     /label=R-ITR
     rep_origin      4556..4862
                     /direction=RIGHT
                     /label=f1 Origin
     misc_feature    5381..6238
                     /label=AmpR
     rep_origin      6389..7056
                     /direction=RIGHT
                     /label=pUC ori
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaagga tctgcgatcg ctccggtgcc
      181 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
      241 aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
      301 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
      361 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc
      421 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc
      481 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta
      541 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc
      601 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct
      661 gttctgcgcc gttacagatc caagctgtga ccggcgccta cggatccgaa gttcctattc
      721 ttcaaatagt ataggaactt cgcagagtag tagctggatt gtagctgcta ttagcaatct
      781 aaaacctctt agaagttcct attcttcaaa aggtatagga acttcggcgc gccttacacc
      841 tcgttctcgt agcagaactt gtacagctcg tccatgccga gagtgatccc ggcggcggtc
      901 acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac
      961 tggtagctca ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc
     1021 tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag
     1081 ttcaccttga tgccgttctt ctgcttgtcg gccatgatat agacgttgtg gctgttgtag
     1141 ttgtactcca gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc
     1201 tcgatgcggt tcaccagggt gtcgccctcg aacttcacct cggcgcgggt cttgtagttg
     1261 ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg
     1321 aagaagtcgt gctgcttcat gtggtcgggg tagcgggcga agcactgcag gccgtagccg
     1381 aaggtggtca cgagggtggg ccagggcacg ggcagctgtg ggacgacatg acttaaccag
     1441 ttaaccatgt tctctgaaca tgagaagggc tggccactct ccagtcgaca taacttcgta
     1501 taggatactt tatacgaagt tatgcagaat ggtagctgga ttgtagctgc tattagcaat
     1561 atgaaacctc ttaataactt cgtatagtat accttatacg aagttatact agttgtgccc
     1621 cccctttttt ttataaaatt gtattaatgt tatatacata tctcctgtat gtgacccatg
     1681 tgcttatgac tctatttctc atgtgtttag gattggcgag cggcctgaca atttccgttc
     1741 ttgaaatgcc agcgggccat tttgcagaag gcagctcagt gatgctggga ggagaagagg
     1801 tagatggtgt agtcaccatg tggggacggt atctcacctg ggcactttcc acgcccatga
     1861 ttctcctcgc tctgggtctc ctggccggaa gcaatgctac aaagctcttc acagctatca
     1921 ctttcgatat cgctatgtgc gtgactggcc ttgccgcggc cctgactacc tcctcccacc
     1981 tcatgagatg gttcttttac gctatcagtt gtgcatgctt tctggtggtc ttgtatatcc
     2041 tgctggtgga gtgggcacag gacgccaaag ccgcgggaac cgctgacatg ttcaataccc
     2101 tgaagctgtt gacagtagtg atgtggctgg ggtatccaat tgtgtgggct cttggagtcg
     2161 agggtatcgc ggtgttgccc gttggggtga cgagctgggg atattctttc ctggatatcg
     2221 tggcaaagta cattttcgca ttcttgctcc tgaactatct gacgtcaaac gaatctgtcg
     2281 tgtccggcag cattttggat gttccatctg cttctgggac cccggctgat gataagagca
     2341 ggatcaccag cgagggcgag tacatccccc tggaccagat cgacatcaac gtgggcgcgc
     2401 ccggctccgg agccacgaac ttctctctgt taaagcaagc aggagacgtg gaagaaaacc
     2461 ccggtcccgt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     2521 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     2581 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caaggtgagt acaggagggg
     2641 taccataact tcgtataaag tatcctatac gaagttattt gccttaaccc agaaattatc
     2701 actgttattc tttagaatgg tgcaaagaat aacttcgtat aaggtatact atacgaagtt
     2761 atgctagcaa ccccgacact tacctgtgta ggaggcaatg ctgacgacag gcacaaggat
     2821 ggttgacaca gcaataagtt ttgcccgtgg atcatcgagt cctcgggtca taaaaacgaa
     2881 cagcagtata ctcagtcctg caagtgcgat gttgatatag agactgcttg caagcaaagg
     2941 gtcgttcagc acgaactcga acaactccct ttgggtaacc tcggcttgaa gggccacggc
     3001 actctcggtc acgggaggca gggtctctgt catggtggcg agctcgaagt tcctatacta
     3061 tttgaagaat aggaacttct tgccttaacc cagaaattat cactgttatt ctttagaatg
     3121 gtgcaaagag aagttcctat accttttgaa gaataggaac ttcgaattcg atatcaagct
     3181 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     3241 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     3301 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     3361 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     3421 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     3481 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     3541 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     3601 gctgctcgcc tatgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     3661 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     3721 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     3781 ataccgagcg ctgctcgaga gatctacggg tggcatccct gtgacccctc cccagtgcct
     3841 ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt
     3901 tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta
     3961 tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca
     4021 agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg
     4081 attctcctgc ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc
     4141 taatttttgt ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac
     4201 tcctaatctc aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga
     4261 accactgctc ccttccctgt ccttctgatt ttgtaggtaa ccacgtgcgg accgagcggc
     4321 cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg
     4381 aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg
     4441 agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt
     4501 gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt
     4561 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     4621 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     4681 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     4741 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     4801 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     4861 aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggc
     4921 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     4981 aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     5041 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     5101 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     5161 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     5221 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     5281 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     5341 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     5401 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     5461 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     5521 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     5581 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     5641 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     5701 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     5761 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     5821 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     5881 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     5941 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     6001 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     6061 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     6121 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     6181 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     6241 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     6301 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     6361 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     6421 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     6481 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     6541 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     6601 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     6661 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     6721 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     6781 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     6841 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     6901 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     6961 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     7021 tttacggttc ctggcctttt gctggccttt tgctcacatg t
//
