LOCUS       Exported                7064 bp ds-DNA     circular SYN 25-NOV-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7064)
  AUTHORS   Charu
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Dec 3, 2019 from SnapGene Viewer 5.0.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7064
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..141
                     /label=L-ITR
                     /note="/vntifkey=19"
     promoter        156..701
                     /label=nEF promoter
                     /label=EF1\promoter
                     /note="/ApEinfo_fwdcolor=cyan"
                     /note="/ApEinfo_revcolor=green"
                     /note="/vntifkey=29"
     misc_feature    708..741
                     /label=FRT
                     /note="/vntifkey=21"
     misc_feature    792..825
                     /label=F5
                     /note="/vntifkey=21"
     misc_feature    834..839
                     /label=KOZAK
                     /note="/vntifkey=21"
     misc_feature    840..1089
                     /label=NpHR Exon 1
                     /label=NpHR\Ex1
                     /note="/vntifkey=21"
     misc_feature    1090..1104
                     /label=CMV intron donor
                     /label=CMV\intron\donor
                     /note="/vntifkey=21"
     misc_feature    1111..1144
                     /label=lox N
                     /label=lox\N
                     /note="/vntifkey=21"
     misc_feature    1195..1228
                     /label=lox 2722
                     /label=lox\2722
                     /note="/vntifkey=21"
     misc_feature    1235..1248
                     /label=CE intron donor
                     /label=CE\intron\donor
                     /note="/vntifkey=21"
     misc_feature    complement(1249..1404)
                     /label=EYFP Exon 2
                     /label=YFP\Intron1
                     /note="/vntifkey=21"
     misc_feature    1405..1461
                     /label=p2A
                     /note="/vntifkey=21"
     misc_feature    1407
                     /label=cleavage
                     /note="/vntifkey=21"
     misc_feature    1462..1470
                     /label=linker
                     /note="/vntifkey=21"
     misc_feature    complement(1480..1539)
                     /label=TS
                     /note="/vntifkey=21"
     misc_feature    complement(1540..2162)
                     /label=NpHR Exon 2
                     /label=NpHR\Ex2
                     /note="/vntifkey=21"
     misc_feature    1876..1878
                     /label=W179F
                     /note="/vntifkey=21"
     misc_feature    2163..2178
                     /label=CMV intron acceptor
                     /label=CMV\intron\acceptor
                     /note="/vntifkey=21"
     misc_feature    complement(2179..2259)
                     /label=CMV bp
                     /label=CMV\bp
                     /note="/vntifkey=21"
     misc_feature    complement(2266..2299)
                     /label=lox N Rev
                     /label=lox\N\Rev
                     /note="/vntifkey=21"
     misc_feature    complement(2350..2383)
                     /label=lox 2722 Rev
                     /label=lox\2722\Rev
                     /note="/vntifkey=21"
     misc_feature    2390..2448
                     /label=CE bp
                     /label=CE\bp
                     /note="/vntifkey=21"
     misc_feature    2449..2457
                     /label=CE acceptor
                     /label=CE\acceptor
                     /note="/vntifkey=21"
     misc_feature    2458..3039
                     /label=EYFP Exon 3
                     /label=YFP\exon\3\with\STOP
                     /note="/vntifkey=21"
     enhancer        3016..3036
                     /label=ER export
                     /label=ER\export
                     /note="/vntifkey=9"
     misc_feature    complement(3049..3082)
                     /label=FRT rev
                     /label=FRT\rev
                     /note="/vntifkey=21"
     misc_feature    complement(3133..3166)
                     /label=F5 rev
                     /label=F5\rev
                     /note="/vntifkey=21"
     misc_feature    3181..3789
                     /label=WPRE
                     /note="/vntifkey=21"
     polyA_signal    3809..4287
                     /label=hGH polyA
                     /label=hGH\polyA
                     /note="/vntifkey=25"
     LTR             4327..4467
                     /label=R-ITR
                     /note="/vntifkey=19"
     rep_origin      4559..4865
                     /direction=RIGHT
                     /label=f1 Origin
                     /label=f1\Origin
                     /note="/vntifkey=33"
     misc_feature    5384..6241
                     /label=AmpR
                     /note="/vntifkey=21"
     rep_origin      6392..7059
                     /direction=RIGHT
                     /label=pUC ori
                     /label=pUC\ori
                     /note="/vntifkey=33"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaagga tctgcgatcg ctccggtgcc
      181 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
      241 aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
      301 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
      361 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc
      421 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc
      481 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta
      541 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc
      601 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct
      661 gttctgcgcc gttacagatc caagctgtga ccggcgccta cggatccgaa gttcctattc
      721 tctagaaagt ataggaactt cgcagagtag tagctggatt gtagctgcta ttagcaatct
      781 aaaacctctt agaagttcct attcttcaaa aggtatagga acttcggcgc gccgccacca
      841 tgacagagac cctgcctccc gtgaccgaga gtgccgtggc ccttcaagcc gaggttaccc
      901 aaagggagtt gttcgagttc gtgctgaacg accctttgct tgcaagcagt ctctatatca
      961 acatcgcact tgcaggactg agtatactgc tgttcgtttt tatgacccga ggactcgatg
     1021 atccacgggc aaaacttatt gctgtgtcaa ccatccttgt gcctgtcgtc agcattgcct
     1081 cctacacagg taagtgtcgg ggttgagctc ataacttcgt atagtatacc ttatacgaag
     1141 ttattctttg caccattcta aagaataaca gtgataattt ctgggttaag gcaaataact
     1201 tcgtatagga tactttatac gaagttatgg tacccctcct gtactcacct tgccggtggt
     1261 gcagatgaac ttcagggtca gcttgccgta ggtggcatcg ccctcgccct cgccggacac
     1321 gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca ccaccccggt
     1381 gaacagctcc tcgcccttgc tcacgggacc ggggttttct tccacgtctc ctgcttgctt
     1441 taacagagag aagttcgtgg ctccggagcc gggcgcgccc acgttgatgt cgatctggtc
     1501 cagggggatg tactcgccct cgctggtgat cctgctctta tcatcagccg gggtcccaga
     1561 agcagatgga acatccaaaa tgctgccgga cacgacagat tcgtttgacg tcagatagtt
     1621 caggagcaag aatgcgaaaa tgtactttgc cacgatatcc aggaaagaat atccccagct
     1681 cgtcacccca acgggcaaca ccgcgatacc ctcgactcca agagcccaca caattggata
     1741 ccccagccac atcactactg tcaacagctt cagggtattg aacatgtcag cggttcccgc
     1801 ggctttggcg tcctgtgccc actccaccag caggatatac aagaccacca gaaagcatgc
     1861 acaactgata gcgtaaaaga accatctcat gaggtgggag gaggtagtca gggccgcggc
     1921 aaggccagtc acgcacatag cgatatcgaa agtgatagct gtgaagagct ttgtagcatt
     1981 gcttccggcc aggagaccca gagcgaggag aatcatgggc gtggaaagtg cccaggtgag
     2041 ataccgtccc cacatggtga ctacaccatc tacctcttct cctcccagca tcactgagct
     2101 gccttctgca aaatggcccg ctggcatttc aagaacggaa attgtcaggc cgctcgccaa
     2161 tcctaaacac atgagaaata gagtcataag cacatgggtc acatacagga gatatgtata
     2221 taacattaat acaattttat aaaaaaaagg gggggcacaa ctagtataac ttcgtataag
     2281 gtatactata cgaagttatt aagaggtttc atattggtaa tagcagctac aatccagcta
     2341 ccattctgca taacttcgta taaagtatcc tatacgaagt tatgtcgact ggagagtggc
     2401 cagcccttct catgttcaga gaacatggtt aactggttaa gtcatgtcgt cccacagctg
     2461 cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgcagtg cttcgcccgc
     2521 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     2581 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     2641 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     2701 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     2761 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     2821 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     2881 ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag
     2941 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     3001 gacgagctgt acaagttctg ctacgagaac gaggtgtaat gagctagcga agttcctata
     3061 ctttctagag aataggaact tctaagcaga caatcttgta atacatcagc agctagtaga
     3121 ttgctccagc tagaagttcc tatacctttt gaagaatagg aacttcgaat tcgatatcaa
     3181 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     3241 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     3301 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     3361 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     3421 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     3481 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     3541 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     3601 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     3661 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     3721 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
     3781 tcgataccga gcgctgctcg agagatctac gggtggcatc cctgtgaccc ctccccagtg
     3841 cctctcctgg ccctggaagt tgccactcca gtgcccacca gccttgtcct aataaaatta
     3901 agttgcatca ttttgtctga ctaggtgtcc ttctataata ttatggggtg gaggggggtg
     3961 gtatggagca aggggcaagt tgggaagaca acctgtaggg cctgcggggt ctattgggaa
     4021 ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa
     4081 gcgattctcc tgcctcagcc tcccgagttg ttgggattcc aggcatgcat gaccaggctc
     4141 agctaatttt tgtttttttg gtagagacgg ggtttcacca tattggccag gctggtctcc
     4201 aactcctaat ctcaggtgat ctacccacct tggcctccca aattgctggg attacaggcg
     4261 tgaaccactg ctcccttccc tgtccttctg attttgtagg taaccacgtg cggaccgagc
     4321 ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca
     4381 ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga
     4441 gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc
     4501 tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc
     4561 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     4621 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     4681 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     4741 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     4801 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     4861 aacaacactc aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc
     4921 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     4981 attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     5041 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     5101 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     5161 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     5221 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     5281 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     5341 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     5401 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     5461 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     5521 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     5581 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     5641 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     5701 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     5761 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     5821 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     5881 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     5941 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     6001 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     6061 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     6121 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     6181 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     6241 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     6301 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     6361 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     6421 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     6481 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     6541 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     6601 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     6661 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     6721 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     6781 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     6841 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     6901 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     6961 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     7021 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//
