LOCUS Exported 6417 bp ds-DNA circular SYN 25-NOV-2019 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6417) AUTHORS Charu TITLE Direct Submission JOURNAL Exported Monday, Dec 2, 2019 from SnapGene Viewer 5.0.6 https://www.snapgene.com COMMENT ORIGDB|GenBank FEATURES Location/Qualifiers source 1..6417 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 1..141 /label=L-ITR /note="/vntifkey=21" promoter 156..701 /label=nEF promoter /label=EF1\promoter /note="/ApEinfo_fwdcolor=cyan" /note="/ApEinfo_revcolor=green" /note="/vntifkey=29" misc_feature 720..725 /label=kozak misc_feature 726..1652 /label=ChRmine /label=E19 /note="/vntifkey=21" misc_feature 726..1060 /label=Exon 1 misc_feature 1061..2008 /label=Exon 2 misc_feature 1662..1721 /label=TS /note="/vntifkey=21" misc_feature 1731..1739 /label=linker /note="/vntifkey=21" misc_feature 1740..1796 /label=p2A /note="/vntifkey=21" misc_feature 1794 /label=cleavage /note="/vntifkey=21" misc_feature 1797..2519 /label=mScarlet /note="/vntifkey=21" misc_feature 2009..2519 /label=Exon 3 misc_feature 2079..2081 /label=E95 enhancer 2493..2513 /label=ER export /label=ER\export /note="/vntifkey=9" misc_feature 2534..3142 /label=WPRE /note="/vntifkey=21" polyA_signal 3162..3640 /label=hGH polyA /label=hGH\polyA /note="/vntifkey=25" misc_feature 3680..3820 /label=R-ITR /note="/vntifkey=21" rep_origin 3912..4218 /direction=RIGHT /label=f1 Origin /label=f1\Origin /note="/vntifkey=33" misc_feature 4737..5594 /label=AmpR /note="/vntifkey=21" rep_origin 5745..6412 /direction=RIGHT /label=pUC ori /label=pUC\ori /note="/vntifkey=33" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtaagga tctgcgatcg ctccggtgcc 181 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc 241 aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac 301 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg 361 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc 421 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc 481 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta 541 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc 601 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct 661 gttctgcgcc gttacagatc caagctgtga ccggcgccta cggatccccg ggtaccggtg 721 ccaccatggc acacgcacca ggcaccgacc agatgttcta cgtgggcaca atggacggct 781 ggtatctgga taccaagctg aactccgtgg ccatcggcgc ccactggtct tgctttatcg 841 tgctgacaat caccacattc tacctgggct atgagtcctg gacatctcgg ggccctagca 901 agagaacctc cttttacgcc ggctatcagg aggagcagaa cctggccctg tttgtgaatt 961 tctttgccat gctgagctac ttcggcaaga tcgtggccga cacactgggc cacaacttcg 1021 gcgatgtggg ccccttcatc atcggcttcg gcaattacag gtatgccgac tacatgctga 1081 cctgcccaat gctggtgtac gatctgctgt atcagctgag ggccccctat cgcgtgtctt 1141 gtagcgccat catctttgcc atcctgatgt ctggcgtgct ggccgagttc tacgcagagg 1201 gcgaccctag gctgaggaat ggcgcctacg cctggtatgg ctttggctgt ttctggttta 1261 tcttcgccta ctctatcgtg atgagcatcg tggccaagca gtatagccgg ctggcccagc 1321 tggcccagga tacaggcgcc gagcacagcc tgcacgtgct gaagttcgcc gtgtttacct 1381 tctccatgct gtggattctg tttcccctgg tgtgggccat ctgccctaga ggcttcggct 1441 ggatcgacga taactggaca gaggtggccc actgcgtgtg cgacatcgtg gccaagtcct 1501 gttacggctt tgccctggcc cggttcagaa agacctatga tgaggagctg tttcggctgc 1561 tggagcagct gggacacgac gaggatgagt tccagaagct ggagctggat atgaggctga 1621 gcagcaatgg cgagcgcctg cggagactgt ctgcggccgc caagagcagg atcaccagcg 1681 agggcgagta catccccctg gaccagatcg acatcaacgt gggcgcgccc ggctccggag 1741 ccacgaactt ctctctgtta aagcaagcag gagacgtgga agaaaacccc ggtcccatgg 1801 tgagcaaggg cgaggcagtg atcaaggagt tcatgcggtt caaggtgcac atggagggct 1861 ccatgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca 1921 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttctcc tgggacatcc 1981 tgtcccctca gttcatgtac ggctccaggg ccttcaccaa gcaccccgcc gacatccccg 2041 actactataa gcagtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg 2101 acggcggcgc cgtgaccgtg acccaggaca cctccctgga ggacggcacc ctgatctaca 2161 aggtgaagct ccgcggcacc aacttccctc ctgacggccc cgtaatgcag aagaagacaa 2221 tgggctggga agcgtccacc gagcggttgt accccgagga cggcgtgctg aagggcgaca 2281 ttaagatggc cctgcgcctg aaggacggcg ggcgctacct ggcggacttc aagaccacct 2341 acaaggccaa gaagcccgtg cagatgcccg gcgcctacaa cgtggaccgc aagttggaca 2401 tcacctccca caacgaggac tacaccgtgg tggaacagta cgaacgctcc gagggccgcc 2461 actccaccgg cggcatggac gagctgtaca agttctgcta cgagaacgag gtgtaatgag 2521 aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga 2581 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt 2641 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt 2701 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg 2761 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg 2821 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc 2881 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat 2941 catcgtcctt tccttggctg ctcgcctatg ttgccacctg gattctgcgc gggacgtcct 3001 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg 3061 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg 3121 ccgcctcccc gcatcgatac cgagcgctgc tcgagagatc tacgggtggc atccctgtga 3181 cccctcccca gtgcctctcc tggccctgga agttgccact ccagtgccca ccagccttgt 3241 cctaataaaa ttaagttgca tcattttgtc tgactaggtg tccttctata atattatggg 3301 gtggaggggg gtggtatgga gcaaggggca agttgggaag acaacctgta gggcctgcgg 3361 ggtctattgg gaaccaagct ggagtgcagt ggcacaatct tggctcactg caatctccgc 3421 ctcctgggtt caagcgattc tcctgcctca gcctcccgag ttgttgggat tccaggcatg 3481 catgaccagg ctcagctaat ttttgttttt ttggtagaga cggggtttca ccatattggc 3541 caggctggtc tccaactcct aatctcaggt gatctaccca ccttggcctc ccaaattgct 3601 gggattacag gcgtgaacca ctgctccctt ccctgtcctt ctgattttgt aggtaaccac 3661 gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc 3721 gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg 3781 gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt 3841 ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc 3901 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac 3961 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg 4021 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt 4081 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc 4141 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct 4201 tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga 4261 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga 4321 attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg 4381 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg 4441 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt 4501 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc 4561 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc 4621 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc 4681 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga 4741 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt 4801 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag 4861 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag 4921 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta 4981 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg 5041 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca 5101 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag 5161 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc 5221 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg 5281 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc 5341 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg 5401 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg 5461 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga 5521 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac 5581 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa 5641 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca 5701 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 5761 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 5821 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 5881 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc 5941 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 6001 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 6061 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 6121 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 6181 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 6241 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 6301 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 6361 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt //