LOCUS       Exported                7109 bp ds-DNA     circular SYN 25-NOV-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7109)
  AUTHORS   Charu
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Dec 3, 2019 from SnapGene Viewer 5.0.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7109
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..141
                     /label=L-ITR
                     /note="/vntifkey=19"
     promoter        156..701
                     /label=nEF promoter
                     /label=EF1\promoter
                     /note="/ApEinfo_fwdcolor=cyan"
                     /note="/ApEinfo_revcolor=green"
                     /note="/vntifkey=29"
     misc_feature    708..741
                     /label=FRT
                     /note="/vntifkey=21"
     misc_feature    792..825
                     /label=F5
                     /note="/vntifkey=21"
     misc_feature    834..839
                     /label=KOZAK
                     /note="/vntifkey=21"
     misc_feature    840..1174
                     /label=ChRmine Exon 1
                     /label=Mo\20\Ex1
                     /note="/vntifkey=21"
     misc_feature    1175..1189
                     /label=CMV intron donor
                     /label=CMV\intron\donor
                     /note="/vntifkey=21"
     misc_feature    1196..1229
                     /label=lox N
                     /label=lox\N
                     /note="/vntifkey=21"
     misc_feature    1280..1313
                     /label=lox 2722
                     /label=lox\2722
                     /note="/vntifkey=21"
     misc_feature    1320..1333
                     /label=CE intron donor
                     /label=CE\intron\donor
                     /note="/vntifkey=21"
     misc_feature    complement(1334..1545)
                     /label=oScarlet Exon 2
                     /label=oScarlet\Ex1
                     /note="/vntifkey=21"
     misc_feature    1546..1602
                     /label=p2A
                     /note="/vntifkey=21"
     misc_feature    1548
                     /label=cleavage
                     /note="/vntifkey=21"
     misc_feature    1603..1611
                     /label=linker
                     /note="/vntifkey=21"
     misc_feature    complement(1621..1680)
                     /label=TS
                     /note="/vntifkey=21"
     misc_feature    complement(1690..2281)
                     /label=ChRmine Exon 2
                     /label=Mo\20\Ex2
                     /note="/vntifkey=21"
     misc_feature    2282..2297
                     /label=CMV intron acceptor
                     /label=CMV\intron\acceptor
                     /note="/vntifkey=21"
     misc_feature    complement(2298..2378)
                     /label=CMV intron branch point
                     /label=CMV\intron\branch\point
                     /note="/vntifkey=21"
     misc_feature    complement(2385..2418)
                     /label=loxN
                     /note="/vntifkey=21"
     misc_feature    complement(2469..2502)
                     /label=lox2722
                     /note="/vntifkey=21"
     misc_feature    2509..2567
                     /label=CE intron BP
                     /label=CE\intron\BP
                     /note="/vntifkey=21"
     misc_feature    2569..2576
                     /label=CE intron acceptor
                     /label=CE\intron\acceptor
                     /note="/vntifkey=21"
     misc_feature    2577..3060
                     /label=oScarlet Exon 3
                     /label=oScarlet\exon\2
                     /note="/vntifkey=21"
     misc_feature    2647..2649
                     /label=E95D
                     /note="/vntifkey=21"
     enhancer        3061..3081
                     /label=ER export
                     /label=ER\export
                     /note="/vntifkey=9"
     misc_feature    3082..3084
                     /label=Stop
                     /note="/vntifkey=21"
     misc_feature    complement(3094..3127)
                     /label=FRT rev
                     /label=FRT\rev
                     /note="/vntifkey=21"
     misc_feature    complement(3178..3211)
                     /label=F5 rev
                     /label=F5\rev
                     /note="/vntifkey=21"
     misc_feature    3226..3834
                     /label=WPRE
                     /note="/vntifkey=21"
     polyA_signal    3854..4332
                     /label=hGH polyA
                     /label=hGH\polyA
                     /note="/vntifkey=25"
     LTR             4372..4512
                     /label=R-ITR
                     /note="/vntifkey=19"
     rep_origin      4604..4910
                     /direction=RIGHT
                     /label=f1 Origin
                     /label=f1\Origin
                     /note="/vntifkey=33"
     misc_feature    5429..6286
                     /label=AmpR
                     /note="/vntifkey=21"
     rep_origin      6437..7104
                     /direction=RIGHT
                     /label=pUC ori
                     /label=pUC\ori
                     /note="/vntifkey=33"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaagga tctgcgatcg ctccggtgcc
      181 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
      241 aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
      301 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
      361 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc
      421 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc
      481 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta
      541 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc
      601 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct
      661 gttctgcgcc gttacagatc caagctgtga ccggcgccta cggatccgaa gttcctattc
      721 tctagaaagt ataggaactt cgcagagtag tagctggatt gtagctgcta ttagcaatct
      781 aaaacctctt agaagttcct attcttcaaa aggtatagga acttcggcgc gccgccacca
      841 tggcacacgc accaggcacc gaccagatgt tctacgtggg cacaatggac ggctggtatc
      901 tggataccaa gctgaactcc gtggccatcg gcgcccactg gtcttgcttt atcgtgctga
      961 caatcaccac attctacctg ggctatgagt cctggacatc tcggggccct agcaagagaa
     1021 cctcctttta cgccggctat caggaggagc agaacctggc cctgtttgtg aatttctttg
     1081 ccatgctgag ctacttcggc aagatcgtgg ccgacacact gggccacaac ttcggcgatg
     1141 tgggcccctt catcatcggc ttcggcaatt acaggtaagt gtcggggttg agctcataac
     1201 ttcgtatagt ataccttata cgaagttatt ctttgcacca ttctaaagaa taacagtgat
     1261 aatttctggg ttaaggcaaa taacttcgta taggatactt tatacgaagt tatggtaccc
     1321 ctcctgtact cacctggagc cgtacatgaa ctgaggggac aggatgtccc aggagaaggg
     1381 cagggggcca cccttggtca ccttcagctt ggcggtctgg gtgccctcgt aggggcggcc
     1441 ctcgccctcg ccctcgatct cgaactcgtg gccgttcatg gagccctcca tgtgcacctt
     1501 gaaccgcatg aactccttga tcactgcctc gcccttgctc accatgggac cggggttttc
     1561 ttccacgtct cctgcttgct ttaacagaga gaagttcgtg gctccggagc cgggcgcgcc
     1621 cacgttgatg tcgatctggt ccagggggat gtactcgccc tcgctggtga tcctgctctt
     1681 ggcggccgca gacagtctcc gcaggcgctc gccattgctg ctcagcctca tatccagctc
     1741 cagcttctgg aactcatcct cgtcgtgtcc cagctgctcc agcagccgaa acagctcctc
     1801 atcataggtc tttctgaacc gggccagggc aaagccgtaa caggacttgg ccacgatgtc
     1861 gcacacgcag tgggccacct ctgtccagtt atcgtcgatc cagccgaagc ctctagggca
     1921 gatggcccac accaggggaa acagaatcca cagcatggag aaggtaaaca cggcgaactt
     1981 cagcacgtgc aggctgtgct cggcgcctgt atcctgggcc agctgggcca gccggctata
     2041 ctgcttggcc acgatgctca tcacgataga gtaggcgaag ataaaccaga aacagccaaa
     2101 gccataccag gcgtaggcgc cattcctcag cctagggtcg ccctctgcgt agaactcggc
     2161 cagcacgcca gacatcagga tggcaaagat gatggcgcta caagacacgc gatagggggc
     2221 cctcagctga tacagcagat cgtacaccag cattgggcag gtcagcatgt agtcggcata
     2281 cctaaacaca tgagaaatag agtcataagc acatgggtca catacaggag atatgtatat
     2341 aacattaata caattttata aaaaaaaggg ggggcacaac tagtataact tcgtataagg
     2401 tatactatac gaagttatta agaggtttca tattgctaat agcagctaca atccagctac
     2461 cattctgcat aacttcgtat aaagtatcct atacgaagtt atgtcgactg gagagtggcc
     2521 agcccttctc atgttcagag aacatggtta actggttaag tcatgtcgtc ccacagggcc
     2581 ttcaccaagc accccgccga catccccgac tactataagc agtccttccc cgagggcttc
     2641 aagtgggacc gcgtgatgaa cttcgaggac ggcggcgccg tgaccgtgac ccaggacacc
     2701 tccctggagg acggcaccct gatctacaag gtgaagctcc gcggcaccaa cttccctcct
     2761 gacggccccg taatgcagaa gaagacaatg ggctgggaag cgtccaccga gcggttgtac
     2821 cccgaggacg gcgtgctgaa gggcgacatt aagatggccc tgcgcctgaa ggacggcggg
     2881 cgctacctgg cggacttcaa gaccacctac aaggccaaga agcccgtgca gatgcccggc
     2941 gcctacaacg tggaccgcaa gttggacatc acctcccaca acgaggacta caccgtggtg
     3001 gaacagtacg aacgctccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     3061 ttctgctacg agaacgaggt gtaatgagct agcgaagttc ctatactttc tagagaatag
     3121 gaacttctaa gcagacaatc ttgtaataca tcagcagcta gtagattgct ccagctagaa
     3181 gttcctatac cttttgaaga ataggaactt cgaattcgat atcaagctta tcgataatca
     3241 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     3301 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     3361 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     3421 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     3481 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     3541 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     3601 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     3661 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     3721 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     3781 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgagcgct
     3841 gctcgagaga tctacgggtg gcatccctgt gacccctccc cagtgcctct cctggccctg
     3901 gaagttgcca ctccagtgcc caccagcctt gtcctaataa aattaagttg catcattttg
     3961 tctgactagg tgtccttcta taatattatg gggtggaggg gggtggtatg gagcaagggg
     4021 caagttggga agacaacctg tagggcctgc ggggtctatt gggaaccaag ctggagtgca
     4081 gtggcacaat cttggctcac tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct
     4141 cagcctcccg agttgttggg attccaggca tgcatgacca ggctcagcta atttttgttt
     4201 ttttggtaga gacggggttt caccatattg gccaggctgg tctccaactc ctaatctcag
     4261 gtgatctacc caccttggcc tcccaaattg ctgggattac aggcgtgaac cactgctccc
     4321 ttccctgtcc ttctgatttt gtaggtaacc acgtgcggac cgagcggccg caggaacccc
     4381 tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac
     4441 caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca
     4501 gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     4561 accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg
     4621 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     4681 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     4741 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     4801 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     4861 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     4921 tatctcgggc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     4981 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
     5041 tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     5101 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     5161 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     5221 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     5281 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     5341 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     5401 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     5461 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     5521 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     5581 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     5641 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     5701 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     5761 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     5821 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     5881 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     5941 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     6001 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     6061 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     6121 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     6181 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     6241 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     6301 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     6361 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     6421 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     6481 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     6541 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     6601 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     6661 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     6721 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     6781 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     6841 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     6901 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     6961 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     7021 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     7081 ggccttttgc tggccttttg ctcacatgt
//
