LOCUS       Exported                6320 bp ds-DNA     circular SYN 25-NOV-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6320)
  AUTHORS   Charu
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Dec 2, 2019 from SnapGene Viewer 5.0.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6320
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..141
                     /label=L-ITR
                     /note="/vntifkey=21"
     promoter        156..701
                     /label=nEF promoter
                     /label=EF1\promoter
                     /note="/ApEinfo_fwdcolor=cyan"
                     /note="/ApEinfo_revcolor=green"
                     /note="/vntifkey=29"
     misc_feature    708..741
                     /label=lox2722
                     /note="/vntifkey=21"
     misc_feature    792..825
                     /label=loxN
                     /note="/vntifkey=21"
     misc_feature    834..848
                     /label=CMV intron donor
                     /label=CMV\intron\donor
                     /note="/vntifkey=21"
     misc_feature    complement(849..1007)
                     /label=EYFP Exon 1
                     /label=eYFP\exon\1
                     /note="/vntifkey=21"
     misc_feature    1005..1007
                     /label=START
                     /note="/vntifkey=21"
     misc_feature    1008..1013
                     /label=kozak
     misc_feature    complement(1020..1053)
                     /label=lox 2722 Rev
                     /label=lox\2722\Rev
                     /note="/vntifkey=21"
     misc_feature    complement(1104..1137)
                     /label=lox N Rev
                     /label=lox\N\Rev
                     /note="/vntifkey=21"
     misc_feature    1144..1224
                     /label=CMV bp
                     /label=CMV\bp
                     /note="/vntifkey=21"
     misc_feature    1233..1266
                     /label=F3
                     /note="/vntifkey=21"
     misc_feature    1317..1350
                     /label=F5
                     /note="/vntifkey=21"
     misc_feature    1357..1370
                     /label=CE intron donor
                     /label=CE\intron\donor
                     /note="/vntifkey=21"
     misc_feature    complement(1371..1517)
                     /label=EYFP Exon 2
                     /label=ex2
                     /note="/vntifkey=21"
     misc_feature    1518..1533
                     /label=CMV intron acceptor
                     /label=CMV\intron\acceptor
                     /note="/vntifkey=21"
     misc_feature    complement(1540..1573)
                     /label=F3 Rev
                     /label=F3\Rev
                     /note="/vntifkey=21"
     misc_feature    complement(1624..1657)
                     /label=F5 Rev
                     /label=F5\Rev
                     /note="/vntifkey=21"
     misc_feature    1664..1722
                     /label=CE bp
                     /label=CE\bp
                     /note="/vntifkey=21"
     misc_feature    1723..1756
                     /label=vLoxP
                     /note="/vntifkey=21"
     misc_feature    1807..1840
                     /label=vLox2272
                     /note="/vntifkey=21"
     misc_feature    complement(1847..2260)
                     /label=EYFP Exon 3
                     /label=ex3
                     /note="/vntifkey=21"
     misc_feature    2261..2268
                     /label=CE intron acceptor
                     /label=CE\intron\acceptor
                     /note="/vntifkey=21"
     misc_feature    complement(2305..2338)
                     /label=vLoxP R
                     /label=vLoxP\R
                     /note="/vntifkey=21"
     V_region        complement(2389..2422)
                     /label=vLox2272 Rev
                     /label=vLox2272\Rev
                     /note="/vntifkey=45"
     misc_feature    2437..3045
                     /label=WPRE
                     /note="/vntifkey=21"
     polyA_signal    3065..3543
                     /label=hGH polyA
                     /label=hGH\polyA
                     /note="/vntifkey=25"
     misc_feature    3583..3723
                     /label=R-ITR
                     /note="/vntifkey=21"
     rep_origin      3815..4121
                     /direction=RIGHT
                     /label=f1 Origin
                     /label=f1\Origin
                     /note="/vntifkey=33"
     misc_feature    4640..5497
                     /label=AmpR
                     /note="/vntifkey=21"
     rep_origin      5648..6315
                     /direction=RIGHT
                     /label=pUC ori
                     /label=pUC\ori
                     /note="/vntifkey=33"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaagga tctgcgatcg ctccggtgcc
      181 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
      241 aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
      301 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
      361 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc
      421 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc
      481 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta
      541 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc
      601 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct
      661 gttctgcgcc gttacagatc caagctgtga ccggcgccta cggatccata acttcgtata
      721 ggatacttta tacgaagtta tgtagctgga gcaatttgaa aagattgttg tattacctct
      781 agctgcgctt aataacttcg tatagtatac cttatacgaa gttatggcgc gccaaccccg
      841 acacttacct tgccggtggt gcagatgaac ttcagggtca gcttgccgta ggtggcatcg
      901 ccctcgccct cgccggacac gctgaacttg tggccgttta cgtcgccgtc cagctcgacc
      961 aggatgggca ccaccccggt gaacagctcc tcgcccttgc tcaccatggt ggcaccggta
     1021 taacttcgta taaagtatcc tatacgaagt tatttgcctt agttattctt agatagaccc
     1081 agaaattatc acttgtgcaa agaataactt cgtataaggt atactatacg aagttatgag
     1141 ctctgtgccc cccctttttt ttataaaatt gtattaatgt tatatacata tctcctgtat
     1201 gtgacccatg tgcttatgac tctattaatt aagaagttcc tattcttcaa atagtatagg
     1261 aacttcgcag aatggtagct ggattgtagc tgctattagc aatatgaaac ctcttagaag
     1321 ttcctattct tcaaaaggta taggaacttc actagtcctc ctgtactcac cttgaagaag
     1381 atggtgcgct cctggacgta gccttcgggc atggcggact tgaagaagtc gtgctgcttc
     1441 atgtggtcgg ggtagcgggc gaagcactgc aggccgtagc cgaaggtggt cacgagggtg
     1501 ggccagggca cgggcagcta aacacatgag aaagtcgacg aagttcctat actatttgaa
     1561 gaataggaac ttcttgcctt aacccagaaa ttatcactgt tattctttag aatggtgcaa
     1621 agagaagttc ctataccttt tgaagaatag gaacttcgct agctggagag tggccagccc
     1681 ttctcatgtt cagagaacat ggttaactgg ttaagtcatg tctcaatttc tgagaactgt
     1741 cattctcgga aattgagtag ctggttgaag attgctctag tattacagca atttgaactg
     1801 cgcttatcaa tttctgagaa gtgtctttct cggaaattga ggtaccttac ttgtacagct
     1861 cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc
     1921 gcttctcgtt ggggtctttg ctcagggcgg actggtagct caggtagtgg ttgtcgggca
     1981 gcagcacggg gccgtcgccg atgggggtgt tctgctggta gtggtcggcg agctgcacgc
     2041 tgccgtcctc gatgttgtgg cggatcttga agttcacctt gatgccgttc ttctgcttgt
     2101 cggccatgat atagacgttg tggctgttgt agttgtactc cagcttgtgc cccaggatgt
     2161 tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg gttcaccagg gtgtcgccct
     2221 cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc ctgtgggatc tagagtcaag
     2281 attgctccag caatcttgta atactcaatt tccgagaatg acagttctca gaaattgata
     2341 agcagacaat cttgtaatac atgcagctag tcaagattgc tccagctatc aatttccgag
     2401 aaagacactt ctcagaaatt gagaattcga tatcaagctt atcgataatc aacctctgga
     2461 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     2521 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     2581 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     2641 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     2701 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     2761 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     2821 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct atgttgccac
     2881 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     2941 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     3001 gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgagcgc tgctcgagag
     3061 atctacgggt ggcatccctg tgacccctcc ccagtgcctc tcctggccct ggaagttgcc
     3121 actccagtgc ccaccagcct tgtcctaata aaattaagtt gcatcatttt gtctgactag
     3181 gtgtccttct ataatattat ggggtggagg ggggtggtat ggagcaaggg gcaagttggg
     3241 aagacaacct gtagggcctg cggggtctat tgggaaccaa gctggagtgc agtggcacaa
     3301 tcttggctca ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc
     3361 gagttgttgg gattccaggc atgcatgacc aggctcagct aatttttgtt tttttggtag
     3421 agacggggtt tcaccatatt ggccaggctg gtctccaact cctaatctca ggtgatctac
     3481 ccaccttggc ctcccaaatt gctgggatta caggcgtgaa ccactgctcc cttccctgtc
     3541 cttctgattt tgtaggtaac cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg
     3601 agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg
     3661 cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc
     3721 aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac
     3781 gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     3841 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     3901 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     3961 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga
     4021 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     4081 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggg
     4141 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     4201 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg
     4261 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     4321 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     4381 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     4441 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     4501 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     4561 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     4621 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     4681 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     4741 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     4801 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     4861 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     4921 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     4981 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     5041 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     5101 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     5161 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     5221 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     5281 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     5341 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     5401 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     5461 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     5521 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     5581 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     5641 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     5701 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     5761 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     5821 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     5881 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     5941 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     6001 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     6061 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     6121 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     6181 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     6241 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     6301 ctggcctttt gctcacatgt
//
