LOCUS       Exported                7569 bp ds-DNA     circular SYN 25-NOV-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7569)
  AUTHORS   Charu
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Dec 2, 2019 from SnapGene Viewer 5.0.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7569
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..141
                     /label=L-ITR
                     /note="/vntifkey=21"
     promoter        156..1419
                     /label=EF-1a
                     /note="/vntifkey=29"
     misc_feature    1426..1459
                     /label=lox2722
                     /note="/vntifkey=21"
     misc_feature    1510..1543
                     /label=loxN
                     /note="/vntifkey=21"
     misc_feature    1552..1566
                     /label=CMV intron donor
                     /label=CMV\intron\donor
                     /note="/vntifkey=21"
     misc_feature    complement(1567..1944)
                     /label=GCaMP6m Exon 1
                     /label=GCamP\6m\Ex1
                     /note="/vntifkey=21"
     misc_feature    1945..1950
                     /label=kozak
     misc_feature    complement(1957..1990)
                     /label=lox 2722 Rev
                     /label=lox\2722\Rev
                     /note="/vntifkey=21"
     misc_feature    complement(2041..2074)
                     /label=lox N Rev
                     /label=lox\N\Rev
                     /note="/vntifkey=21"
     misc_feature    2081..2161
                     /label=CMV bp
                     /label=CMV\bp
                     /note="/vntifkey=21"
     misc_feature    2170..2203
                     /label=F3
                     /note="/vntifkey=21"
     misc_feature    2254..2287
                     /label=F5
                     /note="/vntifkey=21"
     misc_feature    2294..2307
                     /label=CE intron donor
                     /label=CE\intron\donor
                     /note="/vntifkey=21"
     misc_feature    complement(2308..2601)
                     /label=GCaMP6m Exon 2
                     /label=GCaMP\6m\Ex2
                     /note="/vntifkey=21"
     misc_feature    2329
                     /label=SNP C -> A; preserved codon
     misc_feature    2341
                     /label=SNP A->G; preserved codon
     misc_feature    2602..2617
                     /label=CMV intron acceptor
                     /label=CMV\intron\acceptor
                     /note="/vntifkey=21"
     misc_feature    complement(2624..2657)
                     /label=F3 Rev
                     /label=F3\Rev
                     /note="/vntifkey=21"
     misc_feature    complement(2708..2741)
                     /label=F5 Rev
                     /label=F5\Rev
                     /note="/vntifkey=21"
     misc_feature    2748..2806
                     /label=CE bp
                     /label=CE\bp
                     /note="/vntifkey=21"
     misc_feature    2807..2840
                     /label=vLoxP
                     /note="/vntifkey=21"
     misc_feature    2891..2924
                     /label=vLox2272
                     /note="/vntifkey=21"
     misc_feature    complement(2931..3509)
                     /label=GCaMP6m Exon 3
                     /label=GCaMP\6m\Ex3
                     /note="/vntifkey=21"
     misc_feature    3510..3517
                     /label=CE intron acceptor
                     /label=CE\intron\acceptor
                     /note="/vntifkey=21"
     misc_feature    complement(3554..3587)
                     /label=vLoxP R
                     /label=vLoxP\R
                     /note="/vntifkey=21"
     V_region        complement(3638..3671)
                     /label=vLox2272 Rev
                     /label=vLox2272\Rev
                     /note="/vntifkey=45"
     misc_feature    3686..4294
                     /label=WPRE
                     /note="/vntifkey=21"
     polyA_signal    4314..4792
                     /label=hGH polyA
                     /label=hGH\polyA
                     /note="/vntifkey=25"
     misc_feature    4832..4972
                     /label=R-ITR
                     /note="/vntifkey=21"
     rep_origin      5064..5370
                     /direction=RIGHT
                     /label=f1 Origin
                     /label=f1\Origin
                     /note="/vntifkey=33"
     misc_feature    5889..6746
                     /label=AmpR
                     /note="/vntifkey=21"
     rep_origin      6897..7564
                     /direction=RIGHT
                     /label=pUC ori
                     /label=pUC\ori
                     /note="/vntifkey=33"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaagct ttgcaaagat ggataaagtt
      181 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat
      241 tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
      301 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
      361 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
      421 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
      481 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
      541 tacttccact ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      601 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      661 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      721 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      781 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      841 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      901 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      961 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     1021 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     1081 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     1141 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     1201 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1261 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1321 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1381 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatccataac ttcgtatagg
     1441 atactttata cgaagttatg tagctggagc aatttgaaaa gattgttgta ttacctctag
     1501 ctgcgcttaa taacttcgta tagtatacct tatacgaagt tatggcgcgc caaccccgac
     1561 acttaccttg ctcaccatgc tccctccggt accgcccttg tacagctcgt ccatgccgag
     1621 agtgatcccg gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg
     1681 gtctttcgaa agtttggact gcacgctcag gtagtggttg tcgggcagca gcacggggcc
     1741 gtcgccgatg ggggtgttct gctggtagtg gtaggcgagc tgcacgccgc cgtcctcgat
     1801 gttgtggcgg atcttgaagt tcgccttgat gccgttcttc tgcttgtcgg ccttgatata
     1861 gacgttctcg agtgagctca gccgacctat agctctgact gcgtgacctg tcttattcca
     1921 cttacgacgt gatgagtcca ccatggtggc accggtataa cttcgtataa agtatcctat
     1981 acgaagttat ttgccttagt tattcttaga tagacccaga aattatcact tgtgcaaaga
     2041 ataacttcgt ataaggtata ctatacgaag ttatgagctc tgtgcccccc ctttttttta
     2101 taaaattgta ttaatgttat atacatatct cctgtatgtg acccatgtgc ttatgactct
     2161 attaattaag aagttcctat tcttcaaata gtataggaac ttcgcagaat ggtagctgga
     2221 ttgtagctgc tattagcaat atgaaacctc ttagaagttc ctattcttca aaaggtatag
     2281 gaacttcact agtcctcctg tactcacctt gaagaagatg gtgcgctctt ggatgtagcc
     2341 ctcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcggctgaa
     2401 gcactgcacg ccgtaggtca gggtggtcac gagggtgggc cagggcacgg gcagcttgcc
     2461 ggtggtgcag atgaacttca gggtcagctt gccgtaggtg gcatcgccct caccctcgcc
     2521 ggacacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga tgggcaccac
     2581 cccggtgaac agctcctcgc cctaaacaca tgagaaagtc gacgaagttc ctatactatt
     2641 tgaagaatag gaacttcttg ccttaaccca gaaattatca ctgttattct ttagaatggt
     2701 gcaaagagaa gttcctatac cttttgaaga ataggaactt cgctagctgg agagtggcca
     2761 gcccttctca tgttcagaga acatggttaa ctggttaagt catgtctcaa tttctgagaa
     2821 ctgtcattct cggaaattga gtagctggtt gaagattgct ctagtattac agcaatttga
     2881 actgcgctta tcaatttctg agaagtgtct ttctcggaaa ttgaggtacc tcacttcgct
     2941 gtcatcattt gtacaaactc ttcgtagttt acctgaccat ccccatcgat gtctgcttcc
     3001 ctgatcattt catcaacctc ttcatctgtt aacttctctc caaggtttgt catcacgtgg
     3061 cgaagctctg ctgcactgat gtagccattg ccatccttat caaacacacc gaacgcttct
     3121 ctaatttctt cttccgtgtc cctgtagctc ccttttcttg ccatcattgt caggaactca
     3181 gggaagtcga ttgtgccgtc accgtcggca tctacttcat tgatcatgtc ctgcagctct
     3241 gcttctgtgg ggttctgccc cagagaccgc atcaccgtcc ccagctcctt ggttgttatt
     3301 gtcccatccc cgtccttgtc aaatagggag aaagcctctt taaattctgc gatctgctct
     3361 tcagtcagtt ggtccggcag gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc
     3421 ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc
     3481 tcggcgcggg tcttgtagtt gccgtcgtcc tgtgggatct agagtcaaga ttgctccagc
     3541 aatcttgtaa tactcaattt ccgagaatga cagttctcag aaattgataa gcagacaatc
     3601 ttgtaataca tgcagctagt caagattgct ccagctatca atttccgaga aagacacttc
     3661 tcagaaattg agaattcgat atcaagctta tcgataatca acctctggat tacaaaattt
     3721 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     3781 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     3841 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     3901 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     3961 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     4021 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     4081 tgtcggggaa atcatcgtcc tttccttggc tgctcgccta tgttgccacc tggattctgc
     4141 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     4201 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     4261 tctccctttg ggccgcctcc ccgcatcgat accgagcgct gctcgagaga tctacgggtg
     4321 gcatccctgt gacccctccc cagtgcctct cctggccctg gaagttgcca ctccagtgcc
     4381 caccagcctt gtcctaataa aattaagttg catcattttg tctgactagg tgtccttcta
     4441 taatattatg gggtggaggg gggtggtatg gagcaagggg caagttggga agacaacctg
     4501 tagggcctgc ggggtctatt gggaaccaag ctggagtgca gtggcacaat cttggctcac
     4561 tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct cagcctcccg agttgttggg
     4621 attccaggca tgcatgacca ggctcagcta atttttgttt ttttggtaga gacggggttt
     4681 caccatattg gccaggctgg tctccaactc ctaatctcag gtgatctacc caccttggcc
     4741 tcccaaattg ctgggattac aggcgtgaac cactgctccc ttccctgtcc ttctgatttt
     4801 gtaggtaacc acgtgcggac cgagcggccg caggaacccc tagtgatgga gttggccact
     4861 ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg
     4921 ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg
     4981 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac
     5041 catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     5101 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     5161 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     5221 gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta
     5281 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     5341 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcgggc tattcttttg
     5401 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     5461 aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta
     5521 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     5581 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     5641 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     5701 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     5761 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     5821 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     5881 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     5941 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     6001 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     6061 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     6121 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     6181 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     6241 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     6301 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     6361 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     6421 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     6481 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     6541 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     6601 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     6661 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     6721 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     6781 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     6841 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     6901 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     6961 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     7021 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     7081 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     7141 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     7201 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     7261 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     7321 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     7381 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     7441 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     7501 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     7561 ctcacatgt
//
