LOCUS JL637-CMV_Npu-AB 6732 bp DNA circular UNA 28-NOV-2019 DEFINITION . ACCESSION urn.local...bg-b93xovj VERSION urn.local...bg-b93xovj KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers Promoter 275..356 /Imported_from="Pla sMapper" /Transferred_From="CMV prom" /Transferred_Similarity="97.56%" /label="CMV prom" Promoter 377..395 /Imported_from="Pla sMapper" /Transferred_From="T7 prom" /Transferred_Similarity="100.00%" /label="T7 prom" CDS 409..465 /created_by="jonlevy" /Transferred_From="N-terminal BPNLS" /Transferred_Similarity="100.00%" /label="BPNLS" CDS 466..1557 /created_by="jonlevy" /modified_by="jonlevy" /label="ABEmax" CDS 1558..1653 /created_by="jonlevy" /modified_by="jonlevy" /label="GGS-XTEN-GGS Linker" CDS 1654..3369 /note="hSpCas9" /modified_by="jonlevy" /label="hSpCas9" CDS 3370..3675 /created_by="jonlevy" /modified_by="jonlevy" /label="NpuN" CDS 3676..3738 /created_by="jonlevy" /modified_by="jonlevy" /Transferred_From="C-terminal BPNLS" /Transferred_Similarity="100.00%" /label="BPNLS" Terminator 3790..4017 /Imported_from="Pla sMapper" /Transferred_From="bGH_PA term" /Transferred_Similarity="100.00%" /label="bGH_PA term" Promoter complement(4137..4166) /Imported_from="Pla sMapper" /Transferred_From="lac prom" /Transferred_Similarity="100.00%" /label="lac prom" misc_feature complement(4475..5094) /Imported_from="Pla sMapper" /Transferred_From="pBR322 origin" /Transferred_Similarity="99.52%" /label="pBR322 origin" /note="Geneious type: Origin of Replication" misc_feature 4684^4685 /Original_Bases="A" /label="A" /note="Geneious type: Editing History Deletion" misc_feature 4686 /Original_Bases /label /note="Geneious type: Editing History Insertion" misc_feature 4931 /Original_Bases="G" /label="G" /note="Geneious type: Editing History Replacement" Marker complement(5249..6109) /Imported_from="Pla sMapper" /Transferred_From="amp marker" /Transferred_Similarity="99.88%" /label="amp marker" Promoter complement(6151..6179) /Imported_from="Pla sMapper" /Transferred_From="amp prom" /Transferred_Similarity="100.00%" /label="amp prom" promoter 6500..356 /created_by="User" /modified_by="User" /Transferred_From="CMV_Full" /Transferred_Similarity="99.83%" /label="CMV_Full" misc_feature 6689 /Original_Bases="C" /label="C" /note="Geneious type: Editing History Replacement" ORIGIN 1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca 421 gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtctctga agtcgagttt 481 agccacgagt attggatgag gcacgcactg accctggcaa agcgagcatg ggatgaaaga 541 gaagtccccg tgggcgccgt gctggtgcac aacaatagag tgatcggaga gggatggaac 601 aggccaatcg gccgccacga ccctaccgca cacgcagaga tcatggcact gaggcaggga 661 ggcctggtca tgcagaatta ccgcctgatc gatgccaccc tgtatgtgac actggagcca 721 tgcgtgatgt gcgcaggagc aatgatccac agcaggatcg gaagagtggt gttcggagca 781 cgggacgcca agaccggcgc agcaggctcc ctgatggatg tgctgcacca ccccggcatg 841 aaccaccggg tggagatcac agagggaatc ctggcagacg agtgcgccgc cctgctgagc 901 gatttcttta gaatgcggag acaggagatc aaggcccaga agaaggcaca gagctccacc 961 gactctggag gatctagcgg aggatcctct ggaagcgaga caccaggcac aagcgagtcc 1021 gccacaccag agagctccgg cggctcctcc ggaggatcct ctgaggtgga gttttcccac 1081 gagtactgga tgagacatgc cctgaccctg gccaagaggg cacgcgatga gagggaggtg 1141 cctgtgggag ccgtgctggt gctgaacaat agagtgatcg gcgagggctg gaacagagcc 1201 atcggcctgc acgacccaac agcccatgcc gaaattatgg ccctgagaca gggcggcctg 1261 gtcatgcaga actacagact gattgacgcc accctgtacg tgacattcga gccttgcgtg 1321 atgtgcgccg gcgccatgat ccactctagg atcggccgcg tggtgtttgg cgtgaggaac 1381 gcaaaaaccg gcgccgcagg ctccctgatg gacgtgctgc actaccccgg catgaatcac 1441 cgcgtcgaaa ttaccgaggg aatcctggca gatgaatgtg ccgccctgct gtgctatttc 1501 tttcggatgc ctagacaggt gttcaatgct cagaagaagg cccagagctc caccgactcc 1561 ggaggatcta gcggaggctc ctctggctct gagacacctg gcacaagcga gagcgcaaca 1621 cctgaaagca gcgggggcag cagcgggggg tcagacaaga agtacagcat cggcctggcc 1681 atcggcacca actctgtggg ctgggccgtg atcaccgacg agtacaaggt gcccagcaag 1741 aaattcaagg tgctgggcaa caccgaccgg cacagcatca agaagaacct gatcggagcc 1801 ctgctgttcg acagcggcga aacagccgag gccacccggc tgaagagaac cgccagaaga 1861 agatacacca gacggaagaa ccggatctgc tatctgcaag agatcttcag caacgagatg 1921 gccaaggtgg acgacagctt cttccacaga ctggaagagt ccttcctggt ggaagaggat 1981 aagaagcacg agcggcaccc catcttcggc aacatcgtgg acgaggtggc ctaccacgag 2041 aagtacccca ccatctacca cctgagaaag aaactggtgg acagcaccga caaggccgac 2101 ctgcggctga tctatctggc cctggcccac atgatcaagt tccggggcca cttcctgatc 2161 gagggcgacc tgaaccccga caacagcgac gtggacaagc tgttcatcca gctggtgcag 2221 acctacaacc agctgttcga ggaaaacccc atcaacgcca gcggcgtgga cgccaaggcc 2281 atcctgtctg ccagactgag caagagcaga cggctggaaa atctgatcgc ccagctgccc 2341 ggcgagaaga agaatggcct gttcggaaac ctgattgccc tgagcctggg cctgaccccc 2401 aacttcaaga gcaacttcga cctggccgag gatgccaaac tgcagctgag caaggacacc 2461 tacgacgacg acctggacaa cctgctggcc cagatcggcg accagtacgc cgacctgttt 2521 ctggccgcca agaacctgtc cgacgccatc ctgctgagcg acatcctgag agtgaacacc 2581 gagatcacca aggcccccct gagcgcctct atgatcaaga gatacgacga gcaccaccag 2641 gacctgaccc tgctgaaagc tctcgtgcgg cagcagctgc ctgagaagta caaagagatt 2701 ttcttcgacc agagcaagaa cggctacgcc ggctacattg acggcggagc cagccaggaa 2761 gagttctaca agttcatcaa gcccatcctg gaaaagatgg acggcaccga ggaactgctc 2821 gtgaagctga acagagagga cctgctgcgg aagcagcgga ccttcgacaa cggcagcatc 2881 ccccaccaga tccacctggg agagctgcac gccattctgc ggcggcagga agatttttac 2941 ccattcctga aggacaaccg ggaaaagatc gagaagatcc tgaccttccg catcccctac 3001 tacgtgggcc ctctggccag gggaaacagc agattcgcct ggatgaccag aaagagcgag 3061 gaaaccatca ccccctggaa cttcgaggaa gtggtggaca agggcgcttc cgcccagagc 3121 ttcatcgagc ggatgaccaa cttcgataag aacctgccca acgagaaggt gctgcccaag 3181 cacagcctgc tgtacgagta cttcaccgtg tataacgagc tgaccaaagt gaaatacgtg 3241 accgagggaa tgagaaagcc cgccttcctg agcggcgagc agaaaaaggc catcgtggac 3301 ctgctgttca agaccaaccg gaaagtgacc gtgaagcagc tgaaagagga ctacttcaag 3361 aaaatcgagt gcctgtccta cgagacagag atcctgacag tggagtatgg cctgctgcca 3421 atcggcaaga tcgtggagaa gaggatcgag tgtaccgtgt actctgtgga taacaatggc 3481 aacatctata cacagcccgt ggcacagtgg cacgataggg gagagcagga ggtgttcgag 3541 tattgcctgg aggacggcag cctgatcagg gcaaccaagg accacaagtt catgacagtg 3601 gatggccaga tgctgcccat cgacgagatt ttcgagcggg agctggacct gatgagagtg 3661 gataacctgc ctaatagcgg aggcagtaaa agaacagcag acgggagtga gtttgagccc 3721 aagaaaaaga gaaaggtgta accggtcatc atcaccatca ccattgagtt taaacccgct 3781 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc 3841 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg 3901 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca 3961 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt 4021 ctgaggcgga aagaaccagc tggggctcga taccgtcgac ctctagctag agcttggcgt 4081 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 4141 tacgagccgg aagcataaag tgtaaagcct agggtgccta atgagtgagc taactcacat 4201 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 4261 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 4321 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 4381 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 4441 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 4501 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 4561 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 4621 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 4681 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 4741 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 4801 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 4861 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 4921 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 4981 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 5041 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 5101 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 5161 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 5221 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 5281 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 5341 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 5401 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 5461 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 5521 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 5581 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 5641 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 5701 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 5761 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 5821 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 5881 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 5941 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 6001 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 6061 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 6121 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 6181 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 6241 acgtcgacgg atcgggagat cgatctcccg atcccctagg gtcgactctc agtacaatct 6301 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg 6361 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga 6421 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc 6481 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata 6541 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 6601 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 6661 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac 6721 atcaagtgta tc //