LOCUS       JL618-CMV_Npu-AB        6012 bp    DNA     circular UNA 28-NOV-2019
DEFINITION  .
ACCESSION   urn.local...by-b93y06c
VERSION     urn.local...by-b93y06c
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     Promoter        377..395
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7 prom"
                     /Transferred_Similarity="100.00%"
                     /label="T7 prom"
     CDS             409..465
                     /created_by="jonlevy"
                     /Transferred_From="N-terminal BPNLS"
                     /Transferred_Similarity="100.00%"
                     /label="BPNLS"
     CDS             466..570
                     /created_by="jonlevy"
                     /modified_by="jonlevy"
                     /label="NpuC"
     CDS             <571..2955
                     /codon_start=1
                     /note="hSpCas9"
                     /modified_by="jonlevy"
                     /label="hSpCas9"
     CDS             2956..3018
                     /created_by="jonlevy"
                     /label="BPNLS"
     Terminator      3070..3297
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="bGH_PA term"
                     /Transferred_Similarity="100.00%"
                     /label="bGH_PA term"
     Promoter        complement(3417..3446)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="lac prom"
                     /Transferred_Similarity="100.00%"
                     /label="lac prom"
     misc_feature    complement(3755..4374)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="pBR322 origin"
                     /Transferred_Similarity="99.52%"
                     /label="pBR322 origin"
                     /note="Geneious type: Origin of Replication"
     misc_feature    3964^3965
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    3966
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    4211
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     Marker          complement(4529..5389)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="amp marker"
                     /Transferred_Similarity="99.88%"
                     /label="amp marker"
     Promoter        complement(5431..5459)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="amp prom"
                     /Transferred_Similarity="100.00%"
                     /label="amp prom"
     promoter        5780..356
                     /created_by="User"
                     /modified_by="User"
                     /Transferred_From="CMV_Full"
                     /Transferred_Similarity="99.83%"
                     /label="CMV_Full"
     misc_feature    5969
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
ORIGIN      
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca
      421 gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcatcaa gattgctaca
      481 cggaaatacc tgggaaagca gaacgtgtac gacatcggcg tggagcggga tcacaacttc
      541 gccctgaaga atggctttat cgccagcaat tgcttcgact ccgtggaaat ctccggcgtg
      601 gaagatcggt tcaacgcctc cctgggcaca taccacgatc tgctgaaaat tatcaaggac
      661 aaggacttcc tggacaatga ggaaaacgag gacattctgg aagatatcgt gctgaccctg
      721 acactgtttg aggacagaga gatgatcgag gaacggctga aaacctatgc ccacctgttc
      781 gacgacaaag tgatgaagca gctgaagcgg cggagataca ccggctgggg caggctgagc
      841 cggaagctga tcaacggcat ccgggacaag cagtccggca agacaatcct ggatttcctg
      901 aagtccgacg gcttcgccaa cagaaacttc atgcagctga tccacgacga cagcctgacc
      961 tttaaagagg acatccagaa agcccaggtg tccggccagg gcgatagcct gcacgagcac
     1021 attgccaatc tggccggcag ccccgccatt aagaagggca tcctgcagac agtgaaggtg
     1081 gtggacgagc tcgtgaaagt gatgggccgg cacaagcccg agaacatcgt gatcgaaatg
     1141 gccagagaga accagaccac ccagaaggga cagaagaaca gccgcgagag aatgaagcgg
     1201 atcgaagagg gcatcaaaga gctgggcagc cagatcctga aagaacaccc cgtggaaaac
     1261 acccagctgc agaacgagaa gctgtacctg tactacctgc agaatgggcg ggatatgtac
     1321 gtggaccagg aactggacat caaccggctg tccgactacg atgtggacca tatcgtgcct
     1381 cagagctttc tgaaggacga ctccatcgac aacaaggtgc tgaccagaag cgacaagaac
     1441 cggggcaaga gcgacaacgt gccctccgaa gaggtcgtga agaagatgaa gaactactgg
     1501 cggcagctgc tgaacgccaa gctgattacc cagagaaagt tcgacaatct gaccaaggcc
     1561 gagagaggcg gcctgagcga actggataag gccggcttca tcaagagaca gctggtggaa
     1621 acccggcaga tcacaaagca cgtggcacag atcctggact cccggatgaa cactaagtac
     1681 gacgagaatg acaagctgat ccgggaagtg aaagtgatca ccctgaagtc caagctggtg
     1741 tccgatttcc ggaaggattt ccagttttac aaagtgcgcg agatcaacaa ctaccaccac
     1801 gcccacgacg cctacctgaa cgccgtcgtg ggaaccgccc tgatcaaaaa gtaccctaag
     1861 ctggaaagcg agttcgtgta cggcgactac aaggtgtacg acgtgcggaa gatgatcgcc
     1921 aagagcgagc aggaaatcgg caaggctacc gccaagtact tcttctacag caacatcatg
     1981 aactttttca agaccgagat taccctggcc aacggcgaga tccggaagcg gcctctgatc
     2041 gagacaaacg gcgaaaccgg ggagatcgtg tgggataagg gccgggattt tgccaccgtg
     2101 cggaaagtgc tgagcatgcc ccaagtgaat atcgtgaaaa agaccgaggt gcagacaggc
     2161 ggcttcagca aagagtctat cctgcccaag aggaacagcg ataagctgat cgccagaaag
     2221 aaggactggg accctaagaa gtacggcggc ttcgacagcc ccaccgtggc ctattctgtg
     2281 ctggtggtgg ccaaagtgga aaagggcaag tccaagaaac tgaagagtgt gaaagagctg
     2341 ctggggatca ccatcatgga aagaagcagc ttcgagaaga atcccatcga ctttctggaa
     2401 gccaagggct acaaagaagt gaaaaaggac ctgatcatca agctgcctaa gtactccctg
     2461 ttcgagctgg aaaacggccg gaagagaatg ctggcctctg ccggcgaact gcagaaggga
     2521 aacgaactgg ccctgccctc caaatatgtg aacttcctgt acctggccag ccactatgag
     2581 aagctgaagg gctcccccga ggataatgag cagaaacagc tgtttgtgga acagcacaag
     2641 cactacctgg acgagatcat cgagcagatc agcgagttct ccaagagagt gatcctggcc
     2701 gacgctaatc tggacaaagt gctgtccgcc tacaacaagc accgggataa gcccatcaga
     2761 gagcaggccg agaatatcat ccacctgttt accctgacca atctgggagc ccctgccgcc
     2821 ttcaagtact ttgacaccac catcgaccgg aagaggtaca ccagcaccaa agaggtgctg
     2881 gacgccaccc tgatccacca gagcatcacc ggcctgtacg agacacggat cgacctgtct
     2941 cagctgggag gtgactctgg cggctcaaaa agaaccgccg acggcagcga attcgagccc
     3001 aagaagaaga ggaaagtcta accggtcatc atcaccatca ccattgagtt taaacccgct
     3061 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     3121 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     3181 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     3241 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     3301 ctgaggcgga aagaaccagc tggggctcga taccgtcgac ctctagctag agcttggcgt
     3361 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     3421 tacgagccgg aagcataaag tgtaaagcct agggtgccta atgagtgagc taactcacat
     3481 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     3541 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     3601 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     3661 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     3721 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     3781 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     3841 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     3901 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     3961 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     4021 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     4081 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     4141 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     4201 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     4261 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     4321 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     4381 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     4441 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     4501 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     4561 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     4621 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     4681 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     4741 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     4801 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     4861 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     4921 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     4981 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     5041 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     5101 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     5161 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     5221 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     5281 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     5341 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     5401 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     5461 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     5521 acgtcgacgg atcgggagat cgatctcccg atcccctagg gtcgactctc agtacaatct
     5581 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
     5641 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
     5701 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc
     5761 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
     5821 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     5881 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     5941 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     6001 atcaagtgta tc
//
