LOCUS       Exported                8633 bp ds-DNA     circular SYN 02-DEC-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pSHDY_Prha-mVenus_119rhaS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8633)
  AUTHORS   Anna Behle
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Dec 2, 2019 from SnapGene 3.1.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8633
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(7..798)
                     /label=SmR
                     /note="SmR"
     misc_feature    1004..1025
                     /note="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        1026..1147
                     /note="Prha"
     misc_feature    1042..1057
                     /note="CRP binding site"
     misc_feature    1061..1077
                     /note="RhaS O1"
     misc_feature    1094..1109
                     /note="RhaS O2"
     RBS             1156..1165
                     /note="RBS_*"
     CDS             1172..1888
                     /codon_start=1
                     /note="mVenus_SynOpt2_NheI"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     terminator      1950..2021
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2037..2064
                     /note="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     misc_feature    2071..2091
                     /note="BioBrick suffix"
                     /note="universal suffix for all parts"
     CDS             complement(2292..3143)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 from Salmonella enterica serovar 
                     Typhimurium"
                     /note="RSF1010 RepC"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(3130..3969)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 from Salmonella enterica serovar 
                     Typhimurium"
                     /note="RSF1010 RepA"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(4480..6609)
                     /codon_start=1
                     /note="mobA"
                     /translation="MAIYHLTAKTGSRSGGQSARAKADFIQREGKYARDMDEVLHAESG
                     HMPEFVERPADYWDAADLYERANGRLFKEVEFALPVELTLDQQKALASEFAQHLTGAER
                     LPYTLAIHAGGGENPHCHLMISERINDGIERPAAQWFKRYNGKTPEKGGAQKTEALKPK
                     AWLEQTREAWADHANRALERAGHDARIDHRTLEAQGIERLPGVHLGPNVVEMEGRGIRT
                     DRADVALNIDTANAQIIDLQEYREAIDHERNRQSEEIQRHQRVSGADRTAGPEHGDTGR
                     RSPAGHEPDPAGQRGAGGGVAESPAPDRGGMGGAGQRVAGGSRRGEQRRAERPERVAGV
                     ALEAMANRDAGFHDAYGGAADRIVALARPDATDNRGRLDLAALGGPMKNDRTLQAIGRQ
                     LKAMGCERFDIGVRDATTGQMMNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHG
                     LVLVDDLSEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELRGQIARTLASEY
                     DADPASADSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAGPALVQQAGQQI
                     EQAQRQQEKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDDLSKCDFIAAQK
                     LASRGRSAEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQLARAELARAPA
                     PRQRGMDRGGPDFSM"
     CDS             complement(4480..5451)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 from Salmonella enterica serovar 
                     Typhimurium"
                     /note="RSF1010 RepB"
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             complement(5448..5861)
                     /codon_start=1
                     /note="mobB"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
     CDS             complement(5448..5861)
                     /codon_start=1
                     /note="mobB"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
     unsure          6535..6537
                     /note="Y25F"
                     /note="Modification from Taton 2014 to reduce 
                     self-mobilization/ nicked plasmid for more efficient 
                     cloning"
     oriT            6698..6854
     CDS             complement(6808..7092)
                     /codon_start=1
                     /note="mobC"
                     /translation="SARLILLAWRQTEQGAVVVAFKVRIHCRHEPILRPLAAVHLGQNH
                     GPHQHLAPCFVLALLLLFPCPHPLNFGIDSRSLFFELGQPIRRLVAPLNH"
     rep_origin      7119..7513
                     /note="RSF1010 oriV"
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010 from Salmonella enterica serovar Typhimurium; 
                     requires the RSF1010 RepA/B/C proteins for replication"
     misc_feature    7539..7559
                     /note="NeoBrick Prefix"
     promoter        7560..7594
                     /note="J23119 promoter"
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     misc_feature    7595..7606
                     /note="BBa_0034"
     gene            7613..8449
                     /locus_tag="ER3413_RS20180"
                     /old_locus_tag="ER3413_4026"
                     /note="ER3413_RS20180"
     CDS             7613..8449
                     /codon_start=1
                     /transl_table=11
                     /locus_tag="ER3413_RS20180"
                     /old_locus_tag="ER3413_4026"
                     /product="transcriptional activator RhaS"
                     /note="RhaS"
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Protein Homology."
                     /inference="COORDINATES:same species:RefSeq"
                     /protein_id="WP_000217137.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGT
                     GIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQ
                     ELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQ
                     ENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQLYLNR
                     LRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ"
     gene            8433..8449
                     /locus_tag="ER3413_RS20185"
                     /old_locus_tag="ER3413_4028"
                     /note="ER3413_RS20185"
     gene            8450..8614
                     /gene="NA"
                     /locus_tag="slr0271"
                     /product="unknown protein"
                     /note="NA"
                     /note="unknown protein"
                     /db_xref="GeneID:954620"
     terminator      8494..8565
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8581..8608
                     /note="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     misc_feature    8615..1
                     /note="NeoBrick Suffix"
ORIGIN
        1 tagacattat ttgccgacta ccttggtgat ctcgcctttc acgtagtgga caaattcttc
       61 caactgatct gcgcgcgagg ccaagcgatc ttcttcttgt ccaagataag cctgtctagc
      121 ttcaagtatg acgggctgat actgggccgg caggcgctcc attgcccagt cggcagcgac
      181 atccttcggc gcgattttgc cggttactgc gctgtaccaa atgcgggaca acgtaagcac
      241 tacatttcgc tcatcgccag cccagtcggg cggcgagttc catagcgtta aggtttcatt
      301 tagcgcctca aatagatcct gttcaggaac cggatcaaag agttcctccg ccgctggacc
      361 taccaaggca acgctatgtt ctcttgcttt tgtcagcaag atagccagat caatgtcgat
      421 cgtggctggc tcgaagatac ctgcaagaat gtcattgcgc tgccattctc caaattgcag
      481 ttcgcgctta gctggataac gccacggaat gatgtcgtcg tgcacaacaa tggtgacttc
      541 tacagcgcgg agaatctcgc tctctccagg ggaagccgaa gtttccaaaa ggtcgttgat
      601 caaagctcgc cgcgttgttt catcaagcct tacggtcacc gtaaccagca aatcaatatc
      661 actgtgtggc ttcaggccgc catccactgc ggagccgtac aaatgtacgg ccagcaacgt
      721 cggttcgaga tggcgctcga tgacgccaac tacctctgat agttgagtcg atacttcggc
      781 gatcaccgct tccctcatga tgtttaactt tgttttaggg cgactgccct gctgcgtaac
      841 atcgttgctg ctccataaca tcaaacatcg acccacggcg taacgcgctt gctgcttgga
      901 tgcccgaggc atagactgta ccccaaaaaa acagtcataa caagccatga aaaccgccac
      961 tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac caggaattcg cggccgcttc
     1021 tagagccaca attcagcaaa ttgtgaacat catcacgttc atctttccct ggttgccaat
     1081 ggcccatttt cctgtcagta acgagaaggt cgcgaattca ggcgcttttt agactggtcg
     1141 taatgaatac tagagtagtg gaggttacta gatggctagc aaaggagagg aactgtttac
     1201 cggtgtggta cccattttag tagaattgga tggtgatgtg aacggccaca agttcagcgt
     1261 ttccggggaa ggcgaagggg atgcaaccta tggaaagcta actttgaaac tcatttgcac
     1321 taccggtaaa ctccccgtcc cctggcctac gttagtcacg accctcggtt atggtctgca
     1381 atgttttgcc cgttatcccg atcatatgaa acaacacgat ttctttaaat ctgccatgcc
     1441 agagggatat gttcaagaac ggacaatttt ctttaaggat gatggtaatt ataaaacccg
     1501 tgcagaagtc aaatttgaag gtgatacact cgtgaatcgc atcgaactaa aaggtattga
     1561 ctttaaggag gatggaaaca ttctcggcca taagttagaa tacaattaca acagccataa
     1621 tgtgtacatt actgcggata aacaaaaaaa cggtatcaaa gccaacttta aaattcgaca
     1681 taacatcgaa gatggtggcg ttcagttagc ggatcactac caacaaaata ctcccattgg
     1741 agacggacca gtcctgctgc ctgataacca ttatctgtct tatcaatcca aactgtccaa
     1801 ggatccgaat gaaaagcggg atcacatggt tctgttggag tttgtgaccg cggcaggtat
     1861 tactctcgga atggacgaac tatacaaaag gcctgccgcc aacgacgaaa actacgctgc
     1921 atcagtttaa tagtactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     1981 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
     2041 caccttcggg tgggcctttc tgcgtttata tactagtagc ggccgctgca ggagcagaag
     2101 agcatacatc tggaagcaaa gccaggaaag cggcctatgg agctgtgcgg cagcgctcag
     2161 taggcaattt ttcaaaatat tgttaagcct tttctgagca tggtattttt catggtatta
     2221 ccaattagca ggaaaataag ccattgaata taaaagataa aaatgtcttg tttacaatag
     2281 agtggggggg gtcagcctgc cgccttgggc cgggtgatgt cgtacttgcc cgccgcgaac
     2341 tcggttaccg tccagcccag cgcgaccagc tccggcaacg cctcgcgcac ccgctggcgg
     2401 cgcttgcgca tggtcgaacc actggcctct gacggccaga catagccgca caaggtatct
     2461 atggaagcct tgccggtttt gccggggtcg atccagccac acagccgctg gtgcagcagg
     2521 cgggcggttt cgctgtccag cgcccgcacc tcgtccatgc tgatgcgcac atgctggccg
     2581 ccacccatga cggcctgcgc gatcaagggg ttcagggcca cgtacaggcg cccgtccgcc
     2641 tcgtcgctgg cgtactccga cagcagccga aacccctgcc gcttgcggcc attctgggcg
     2701 atgatggata ccttccaaag gcgctcgatg cagtcctgta tgtgcttgag cgccccacca
     2761 ctatcgacct ctgccccgat ttcctttgcc agcgcccgat agctaccttt gaccaccatg
     2821 gcatcagcgg tgacggcctc ccacttgggt tccaggaaca gccggagctg ccgtccgcct
     2881 tcggtcttgg gttccgggcc aagcactagg ccattaggcc cagccatggc caccagccct
     2941 tgcaggatgc gcagatcatc agcgcccagc ggctccgggc cgctgaactc gatccgcttg
     3001 ccgtcgccgt agtcatacgt cacgtccagc ttgctgcgct tgcgctcgcc ccgcttgagg
     3061 gcacggaaca ggccgggggc cagacagtgc gccgggtcgt gccggacgtg gctgaggctg
     3121 tgcttgttct taggcttcac cacggggcac ccccttgctc ttgcgctgcc tctccagcac
     3181 ggcgggcttg agcaccccgc cgtcatgccg cctgaaccac cgatcagcga acggtgcgcc
     3241 atagttggcc ttgctcacac cgaagcggac gaagaaccgg cgctggtcgt cgtccacacc
     3301 ccattcctcg gcctcggcgc tggtcatgct cgacaggtag gactgccagc ggatgttatc
     3361 gaccagtacc gagctgcccc ggctggcctg ctgctggtcg cctgcgccca tcatggccgc
     3421 gcccttgctg gcatggtgca ggaacacgat agagcacccg gtatcggcgg cgatggcctc
     3481 catgcgaccg atgacctggg ccatggggcc gctggcgttt tcttcctcga tgtggaaccg
     3541 gcgcagcgtg tccagcacca tcaggcggcg gccctcggcg gcgcgcttga ggccgtcgaa
     3601 ccactccggg gccatgatgt tgggcaggct gccgatcagc ggctggatca gcaggccgtc
     3661 agccacggct tgccgttcct cggcgctgag gtgcgcccca agggcgtgca ggcggtgatg
     3721 aatggcggtg ggcgggtctt cggcgggcag gtagatcacc gggccggtgg gcagttcgcc
     3781 cacctccagc agatccggcc cgcctgcaat ctgtgcggcc agttgcaggg ccagcatgga
     3841 tttaccggca ccaccgggcg acaccagcgc cccgaccgta ccggccacca tgttgggcaa
     3901 aacgtagtcc agcggtggcg gcgctgctgc gaacgcctcc agaatattga taggcttatg
     3961 ggtagccatt gattgcctcc tttgcaggca gttggtggtt aggcgctggc ggggtcacta
     4021 cccccgccct gcgccgctct gagttcttcc aggcactcgc gcagcgcctc gtattcgtcg
     4081 tcggtcagcc agaacttgcg ctgacgcatc cctttggcct tcatgcgctc ggcatatcgc
     4141 gcttggcgta cagcgtcagg gctggccagc aggtcgccgg tctgcttgtc cttttggtct
     4201 ttcatatcag tcaccgagaa acttgccggg gccgaaaggc ttgtcttcgc ggaacaagga
     4261 caaggtgcag ccgtcaaggt taaggctggc catatcagcg actgaaaagc ggccagcctc
     4321 ggccttgttt gacgtataac caaagccacc gggcaaccaa tagcccttgt cacttttgat
     4381 caggtagacc gaccctgaag cgcttttttc gtattccata aaaccccctt ctgtgcgtga
     4441 gtactcatag tataacaggc gtgagtacca acgcaagcac tacatgctga aatctggccc
     4501 gcccctgtcc atgcctcgct ggcggggtgc cggtgcccgt gccagctcgg cccgcgcaag
     4561 ctggacgctg ggcagaccca tgaccttgct gacggtgcgc tcgatgtaat ccgcttcgtg
     4621 gccgggcttg cgctctgcca gcgctgggct ggcctcggcc atggccttgc cgatttcctc
     4681 ggcactgcgg ccccggctgg ccagcttctg cgcggcgata aagtcgcact tgctgaggtc
     4741 atcaccgaag cgcttgacca gcccggccat ctcgctgcgg tactcgtcca gcgccgtgcg
     4801 ccggtggcgg ctaagctgcc gctcgggcag ttcgaggctg gccagcctgc gggccttctc
     4861 ctgctgccgc tgggcctgct cgatctgctg gccagcctgc tgcaccagcg ccgggccagc
     4921 ggtggcggtc ttgcccttgg attcacgcag cagcacccac ggctgataac cggcgcgggt
     4981 ggtgtgcttg tccttgcggt tggtgaagcc cgccaagcgg ccatagtggc ggctgtcggc
     5041 gctggccggg tcggcgtcgt actcgctggc cagcgtccgg gcaatctgcc cccgaagttc
     5101 accgcctgcg gcgtcggcca ccttgaccca tgcctgatag ttcttcgggc tggtttccac
     5161 taccagggca ggctcccggc cctcggcttt catgtcatcc aggtcaaact cgctgaggtc
     5221 gtccaccagc accagaccat gccgctcctg ctcggcgggc ctgatataca cgtcattgcc
     5281 ctgggcattc atccgcttga gccatggcgt gttctggagc acttcggcgg ctgaccattc
     5341 ccggttcatc atctggccgg tggtggcgtc cctgacgccg atatcgaagc gctcacagcc
     5401 catggccttg agctgtcggc ctatggcctg caaagtcctg tcgttcttca tcgggccacc
     5461 aagcgcagcc agatcgagcc gtcctcggtt gtcagtggcg tcaggtcgag caagagcaac
     5521 gatgcgatca gcagcaccac cgtaggcatc atggaagcca gcatcacggt tagccatagc
     5581 ttccagtgcc acccccgcga cgcgctccgg gcgctctgcg cggcgctgct cacctcggcg
     5641 gctacctccc gcaactcttt ggccagctcc acccatgccg cccctgtctg gcgctgggct
     5701 ttcagccact ccgccgcctg cgcctcgctg gcctgctggg tctggctcat gacctgccgg
     5761 gcttcgtcgg ccagtgtcgc catgctctgg gccagcggtt cgatctgctc cgctaactcg
     5821 ttgatgcctc tggatttctt cactctgtcg attgcgttca tggtctattg cctcccggta
     5881 ttcctgtaag tcgatgatct gggcgttggc ggtgtcgatg ttcagggcca cgtctgcccg
     5941 gtcggtgcgg atgccccggc cttccatctc caccacgttc ggccccaggt gaacaccggg
     6001 caggcgctcg atgccctgcg cctcaagtgt tctgtggtca atgcgggcgt cgtggccagc
     6061 ccgctctaat gcccggttgg catggtcggc ccatgcctcg cgggtctgct caagccatgc
     6121 cttgggcttg agcgcttcgg tcttctgtgc cccgcccttc tccggggtct tgccgttgta
     6181 ccgcttgaac cactgagcgg cgggccgctc gatgccgtca ttgatccgct cggagatcat
     6241 caggtggcag tgcgggttct cgccgccacc ggcatggatg gccagcgtat acggcaggcg
     6301 ctcggcaccg gtcaggtgct gggcgaactc ggacgccagc gccttctgct ggtcgagggt
     6361 cagctcgacc ggcagggcaa attcgacctc cttgaacagc cgcccattgg cgcgttcata
     6421 caggtcggca gcatcccagt agtcggcggg ccgctcgacg aactccggca tgtgcccgga
     6481 ttcggcgtgc aagacttcat ccatgtcgcg ggcatacttg ccttcgcgct ggatgaagtc
     6541 ggccttggcc ctggccgatt ggccgcccga cctgctgccg gttttcgccg taaggtgata
     6601 aatcgccatg ctgcctcgct gttgcttttg cttttcggct ccatgcaatg gccctcggag
     6661 agcgcaccgc ccgaagggtg gccgttaggc cagtttctcg aagagaaacc ggtaagtgcg
     6721 ccctccccta caaagtaggg tcgggattgc cgccgctgtg cctccatgat agcctacgag
     6781 acagcacatt aacaatgggg tgtcaagatg gttaagggga gcaacaaggc ggcggatcgg
     6841 ctggccaagc tcgaagaaca acgagcgcga atcaatgccg aaattcagcg ggtgcgggca
     6901 agggaacagc agcaagagcg caagaacgaa acaaggcgca aggtgctggt gggggccatg
     6961 attttggcca aggtgaacag cagcgagtgg ccggaggatc ggctcatggc ggcaatggat
     7021 gcgtaccttg aacgcgacca cgaccgcgcc ttgttcggtc tgccgccacg ccagaaggat
     7081 gagccgggct gaatgatcga ccgagacagg ccctgcgggg ctgcacacgc gcccccaccc
     7141 ttcgggtagg gggaaaggcc gctaaagcgg ctaaaagcgc tccagcgtat ttctgcgggg
     7201 tttggtgtgg ggtttagcgg gctttgcccg cctttccccc tgccgcgcag cggtggggcg
     7261 gtgtgtagcc tagcgcagcg aatagaccag ctatccggcc tctggccggg catattgggc
     7321 aagggcagca gcgccccaca agggcgctga taaccgcgcc tagtggatta ttcttagata
     7381 atcatggatg gatttttcca acaccccgcc agcccccgcc cctgctgggt ttgcaggttt
     7441 gggggcgtga cagttattgc aggggttcgt gacagttatt gcaggggggc gtgacagtta
     7501 ttgcaggggt tcgtgacagt tagtacggga gtgacggggg taccgaggtc gatgtcgact
     7561 tgacagctag ctcagtccta ggtataatgc tagcaaagag gagaaatact agatgaccgt
     7621 attacatagt gtggattttt ttccgtctgg taacgcgtcc gtggcgatag aaccccggct
     7681 cccgcaggcg gattttcctg aacatcatca tgattttcat gaaattgtga ttgtcgaaca
     7741 tggcacgggt attcatgtgt ttaatgggca gccctatacc atcaccggtg gcacggtctg
     7801 tttcgtacgc gatcatgatc ggcatctgta tgaacatacc gataatctgt gtctgaccaa
     7861 tgtgctgtat cgctcgccgg atcgatttca gtttctcgcc gggctgaatc agttgctgcc
     7921 acaagagctg gatgggcagt atccgtctca ctggcgcgtt aaccacagcg tattgcagca
     7981 ggtgcgacag ctggttgcac agatggaaca gcaggaaggg gaaaatgatt taccctcgac
     8041 cgccagtcgc gagatcttgt ttatgcaatt actgctcttg ctgcgtaaaa gcagtttgca
     8101 ggagaacctg gaaaacagcg catcacgtct caacttgctt ctggcctggc tggaggacca
     8161 ttttgccgat gaggtgaatt gggatgccgt ggcggatcaa ttttctcttt cactgcgtac
     8221 gctacatcgg cagcttaagc agcaaacggg actgacgcct cagctatacc tgaaccgcct
     8281 gcgactgatg aaagcccgac atctgctacg ccacagcgag gccagcgtta ctgacatcgc
     8341 ctatcgctgt ggattcagcg acagtaacca cttttcgacg ctttttcgcc gagagtttaa
     8401 ctggtcaccg cgtgatattc gccagggacg ggatggcttt ctgcaataat aacactgata
     8461 gtgctagtgt agatcactac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa
     8521 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact
     8581 ggctcacctt cgggtgggcc tttctgcgtt tatactcgag agcggccgaa gct
//
