LOCUS       Exported                5285 bp ds-DNA     circular SYN 12-JAN-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 5285)
  AUTHORS   DOminik Niopek
  TITLE     Direct Submission
  JOURNAL   Exported Jan 12, 2018 from SnapGene 4.1.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5285
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     primer_bind     625..647
                     /label=T7 Primer
                     /note="ApEinfo_fwdcolor=pink
                     vntifkey=28
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
     misc_feature    690..830
                     /label=AAV-2 ITR
                     /note="ApEinfo_fwdcolor=pink
                     vntifkey=21
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
     promoter        849..1470
                     /label=RSV
                     /note="ApEinfo_fwdcolor=pink
                     vntifkey=29
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
     CDS             1492..2226
                     /codon_start=1
                     /label=gfp
                     /note="ApEinfo_fwdcolor=pink
                     vntifkey=4
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
                     /translation="TGRHHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGRGRCHLR
                     QADPEVHLHHRQAARALAHPRDHPDLRRAVLQPLPRPHEAARLLQVRHARRLRPGAHHL
                     LQGRRQLQDPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLQQPQRLYHGRQAE
                     ERHQGELQDPPQHRGRQRAARRPLPAEHPHRRRPRVAARQPLPEHPVRPEQRPQREARS
                     HGPAGVRDRRRDHSRHGRAVQVK"
     polyA_site      2233..2276
                     /label=minimal polyA
                     /note="ApEinfo_fwdcolor=pink
                     vntifkey=26
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
     primer_bind     2297..2321
                     /label=#843 MH_SpScaffoldFE_XbaI_re
     polyA_signal    2297..2302
                     /label=6xT
                     /note="ApEinfo_fwdcolor=pink
                     vntifkey=25
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
     misc_feature    2303..2385
                     /label=F+E Sp scaffold
                     /note="ApEinfo_fwdcolor=#0080c0
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
     misc_feature    2303..2385
                     /label=misc_feature
                     /note="3'overhang=GGCC
                     ugene_name=Fragment 3
                     5'overhang=GTAC
                     3'type=sticky
                     fragment_source=_0443 pBS H1 GFP
                     ugene_group=fragments
                     ApEinfo_fwdcolor=pink
                     3'strand=rev-compl
                     3'term=NotI
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink
                     5'term=KpnI
                     5'strand=rev-compl
                     5'type=sticky"
     promoter        2415..2886
                     /label=U6
                     /note="ApEinfo_fwdcolor=pink
                     vntifkey=29
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
     misc_feature    2415..2659
                     /label=short U6 Variant FZ
     promoter        complement(2419..2659)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     primer_bind     complement(2611..2642)
                     /label=727
     primer_bind     complement(2864..2886)
                     /label=#842 MH_U6_MluI_fw
     misc_feature    2907..3047
                     /label=AAV-4 ITR
                     /note="ApEinfo_fwdcolor=pink
                     vntifkey=21
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
     primer_bind     3096..3115
                     /label=T3 Primer
                     /note="ApEinfo_fwdcolor=pink
                     vntifkey=28
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
     CDS             complement(4297..5157)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5158..5262)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctctc
      661 tagaatgcag gggggggggg gggggggggc cactccctct ctgcgcgctc gctcgctcac
      721 tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag
      781 cgagcgagcg cgcagagagg gagtggccaa ctccatcact aggggttcct agatctgata
      841 tcgtcgacca attctcatgt ttgacagctt atcatcgcag atccgtatgg tgcactctca
      901 gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg
      961 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca
     1021 attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca
     1081 gatatacgcg tatctgaggg gactagggtg tgtttaggcg aaaagcgggg cttcggttgt
     1141 acgcggttag gagtcccctc aggatatagt agtttcgctt ttgcataggg agggggaaat
     1201 gtagtcttat gcaaatactc ttggtagtct tgcaacatgg taaggatgag ttagcaacat
     1261 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
     1321 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattc
     1381 cgcattgcag agatattgta tttaagtgcc tagctcgata caataaacgc catttgacca
     1441 ttcaccacat tggtgtgcac ctccaagctg ggtaccgcgg gcccgggatc caccggtcgc
     1501 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     1561 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     1621 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     1681 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     1741 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     1801 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     1861 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     1921 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     1981 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     2041 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgttgc tgcccgacaa
     2101 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     2161 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     2221 gtaaaggcta gcaataaagg atcgtttatt ttcattggaa gcgtgtgttg gttttttgat
     2281 cactcgaggc ggccgcaaaa aaagcaccga ctcggtgcca ctttttcaag ttgataacgg
     2341 actagcctta tttaaacttg ctatgctgtt tccagcatag ctcttaaaca accctaaccc
     2401 taaccctaac ggtgtttcgt cctttccaca agatatataa agccaagaaa tcgaaatact
     2461 ttcaagttac ggtaagcata tgatagtcca ttttaaaaca taattttaaa actgcaaact
     2521 acccaagaaa ttattacttt ctacgtcacg tattttgtac taatatcttt gtgtttacag
     2581 tcaaattaat tctaattatc tctctaacag ccttgtatcg tatatgcaaa tatgaaggaa
     2641 tcatgggaaa taggccctct tcctgcccga ccttggcgcg cgctcggcgc gcggtcacgc
     2701 tccgtcacgt ggtgcgtttt gcctgcgcgt ctttccactg gggccggggc ggcgcggagg
     2761 gttcagacgc gggctcttac tcaaacgctg atccccgaaa ctaccgagca gtgtctgcgc
     2821 gacgcgaaag catttttgga gcgaggggcc atggtgccca tgggattccc acgggtgttg
     2881 gactcgggcg cgccgatatc agatctggca aacctagatg atggaggtac ccactccctc
     2941 tatgcgcgct cgctcactca ctcggccctg ccggccagag gccggcagtc tggagacctt
     3001 tggtctccag ggccgagtga gtgagcgagc gcgcatagag ggagtgggta ggacgcgtcc
     3061 tgcaggatgc atactagtgg tacccagctt ttgttccctt tagtgagggt taattgcgcg
     3121 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     3181 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     3241 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     3301 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     3361 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     3421 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     3481 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     3541 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     3601 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     3661 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     3721 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     3781 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     3841 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     3901 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     3961 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     4021 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     4081 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     4141 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     4201 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     4261 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     4321 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     4381 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     4441 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     4501 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     4561 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     4621 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     4681 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     4741 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     4801 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     4861 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     4921 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     4981 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     5041 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     5101 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     5161 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     5221 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     5281 gccac
//
