LOCUS Exported 11937 bp ds-DNA circular SYN 06-DEC-2019 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS LentiGuide-Puro-P2A-EGFP_mRFPstuf SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11937) AUTHORS . TITLE Direct Submission JOURNAL Exported Saturday, Dec 7, 2019 from SnapGene Viewer 5.0.5 https://www.snapgene.com FEATURES Location/Qualifiers source 1..11937 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 87..1265 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" CDS 1278..1874 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" CDS 1884..1940 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" CDS 1941..2657 /codon_start=1 /product="enhanced GFP" /label=EGFP /note="mammalian codon-optimized" /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" misc_feature 2673..3261 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" LTR 3333..3566 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 3644..3765 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin 3805..3940 /label=SV40 ori /note="SV40 origin of replication" promoter complement(3961..3979) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(3989..4005) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" rep_origin 4147..4602 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 4628..4732 /gene="bla" /label=AmpR promoter CDS 4733..5593 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 5764..6352 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 6640..6661 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 6676..6706 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6714..6730 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 6738..6754 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" promoter 6775..6793 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 6821..7047 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 7048..7228 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 7275..7400 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 7893..8126 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." promoter 8533..8773 /label=U6 promoter /note="RNA polymerase III promoter for human U6 snRNA" misc_feature 8778..11640 /label=Stuffer Sequence protein_bind 9440..9461 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 9476..9506 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9514..9530 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." RBS 9548..9559 /note="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)" CDS 9566..10243 /codon_start=1 /product="monomeric derivative of DsRed (Campbell et al., 2002)" /label=mRFP1 /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS TGA" terminator 10288..10359 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" misc_feature 11598..11640 /label=Stuffer misc_RNA 11637..11712 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" misc_feature 11768..11885 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" ORIGIN 1 cccgggtgca aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct 61 tctaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat 121 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgatccggt gcctagagaa 181 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg 241 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt 301 ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg 361 gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc 421 ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt 481 cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt 541 ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat 601 gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc 661 acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca 721 catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc 781 aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg 841 cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc 901 ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac 961 ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt 1021 accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag 1081 gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag 1141 ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat 1201 cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt 1261 cgtgacgtac ggccaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg 1321 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc 1381 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca 1441 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gccgtggcgg 1501 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca 1561 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc 1621 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggagtctcg cccgaccacc 1681 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg 1741 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg 1801 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc 1861 gcaagcccgg tgccggatcc ggcgcaacaa acttctctct gctgaaacaa gccggagatg 1921 tcgaagagaa tcctggaccg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca 1981 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg 2041 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc 2101 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct 2161 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc 2221 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt 2281 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg 2341 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg 2401 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg 2461 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc 2521 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga 2581 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg 2641 acgagctgta caagtaaacg cgttaagtcg acaatcaacc tctggattac aaaatttgtg 2701 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt 2761 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata 2821 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg 2881 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc 2941 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct 3001 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt 3061 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg 3121 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc 3181 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct 3241 ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg cagctgtaga 3301 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag 3361 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga 3421 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct 3481 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt 3541 ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc ttattattca 3601 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc 3661 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 3721 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta 3781 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct 3841 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct 3901 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc 3961 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg 4021 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc 4081 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg 4141 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg 4201 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc 4261 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc 4321 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta 4381 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta 4441 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg 4501 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa 4561 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg 4621 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct 4681 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat 4741 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc 4801 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg 4861 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg 4921 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga 4981 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta 5041 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc 5101 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc 5161 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg 5221 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc 5281 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca 5341 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct 5401 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat 5461 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg 5521 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat 5581 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact 5641 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat 5701 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 5761 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct 5821 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg 5881 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca 5941 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc 6001 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga 6061 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac 6121 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga 6181 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag 6241 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg 6301 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag 6361 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc 6421 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc 6481 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc 6541 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag 6601 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca 6661 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag 6721 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa 6781 ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc 6841 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag 6901 caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa 6961 cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta 7021 tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg 7081 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg 7141 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc 7201 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag 7261 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag 7321 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga 7381 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa 7441 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca 7501 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac 7561 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat 7621 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg 7681 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg 7741 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat 7801 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga 7861 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga 7921 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta 7981 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat 8041 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa 8101 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc 8161 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat 8221 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc 8281 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat 8341 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta 8401 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata 8461 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg 8521 aggggaccca gagagggcct atttcccatg attccttcat atttgcatat acgatacaag 8581 gctgttagag agataattag aattaatttg actgtaaaca caaagatatt agtacaaaat 8641 acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa 8701 atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc 8761 ttgtggaaag gacgaaacac cggagacggt tgtaaatgag cacacaaaat acacatgcta 8821 aaatattata ttctatgacc tttataaaat caaccaaaat cttcttttta ataactttag 8881 tatcaataat tagaattttt atgttccttt ttgcaaactt ttaataaaaa tgagcaaaat 8941 aaaaaaacgc tagttttagt aactcgcgtt gttttcttca cctttaataa tagctactcc 9001 accacttgtt cctaagcggt cagctcctgc ttcaatcatt ttttgagcat cttcaaatgt 9061 tctaactcca ccagctgctt taactaaagc attgtcttta acaactgact tcattagttt 9121 aacatcttca aatgttgcac ctgattttga aaatcctgtt gatgttttaa caaattctaa 9181 tccagcttca acagctattt cacaagcttt catgatttct tcttttgtta ataaacaatt 9241 ttccataata catttaacaa catgtgatcc agctgctttt tttacagctt tcatgtcttc 9301 taaaactaat tcataatttt tgtcttttaa tgcaccaata tttaatacca tatcaatttc 9361 tgttgcacca tctttaattg cttcagaact ggcacgacag gtttcccgac tggaaagcgg 9421 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac 9481 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacat 9541 actagagaaa gaggagaaat actagatggc ttcctccgag gacgttatca aagagttcat 9601 gcgtttcaaa gttcgtatgg aaggttccgt taacggtcac gagttcgaaa tcgaaggtga 9661 aggtgaaggt cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg 9721 tccgctgccg ttcgcttggg acatcctgtc cccgcagttc cagtacggtt ccaaagctta 9781 cgttaaacac ccggctgaca tcccggacta cctgaaactg tccttcccgg aaggtttcaa 9841 atgggaacgt gttatgaact tcgaggacgg tggtgttgtt accgttaccc aggactcctc 9901 cctgcaagac ggtgagttca tctacaaagt taaactgcgt ggtaccaact tcccgtccga 9961 cggtccggtt atgcagaaaa aaaccatggg ttgggaagct tccaccgaac gtatgtaccc 10021 ggaggacggt gctctgaaag gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca 10081 ctacgacgct gaagttaaaa ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc 10141 ttacaaaacc gacatcaaac tggacatcac ctcccacaac gaggactaca ccatcgttga 10201 acagtacgaa cgtgctgaag gtcgtcactc caccggtgct taataacgct gatagtgcta 10261 gtgtagatcg ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 10321 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggtgta 10381 gctgttgtgc atgcacctag aggaaaacct acaacatttg ttattcctac atttgtgcct 10441 tttaataatt ctttacaata gcttgttcaa tatgaattaa cacaaactgt tgcaaaatca 10501 aattcaattg cttcatcaca taattgttta atttcagctt tcgtagcatc ttgttttaat 10561 aatgtgtgat ctatatattt gtttagtttc attttttctc ctatatattc atttttaatt 10621 ttaattcttt aataatttcg tctactttaa ctttagcgtt ttgaacagat tcaccaacac 10681 ctataaaata aatttttagt ttaggttcag ttccacttgg gcgaacagca aatcatgact 10741 tatcttctaa ataaaatttt agtaagtctt gtcctggcat attatacatt ccatcgatgt 10801 agtcttcaac attaacaact ttaagtccag caatttgagt taagggtgtt gctctcaatg 10861 atttcattaa tggttcaatt tttaatttct tttcttctgg tttaaaattc aagtttaaag 10921 tgaaagtgta atatgcaccc atttctttaa ataaatcttc taaatagtct actaatgttt 10981 tattttgttt tttataaaat caagcagcct ctgctattaa tatagaagct tgtattccat 11041 ctttatctct agctgagtca tcaattacat atccataact ttcttcataa gcaaaaacaa 11101 aatttaatcc gttatcttct tctttagcaa tttctctacc cattcattta aatccagtta 11161 aagtttttac aatattaact ccatattttt catgagcgat tctatcaccc aaatcacttg 11221 ttacaaaact tgaatataga gccggatttt ttggaatgct atttaagcgt tttagatttg 11281 ataattttca atcaattaaa attggtcctg tttgatttcc atctaatctt acaaaatgac 11341 catcatgttt tattgccatt ccaaatctgt cagcatctgg gtcattcata ataataatat 11401 ctgcatcatg tttaatacca tattcaagcg gtatttttca tgcaggatca aattctggat 11461 ttggatttac aacattttta aatgtttcat cttcaaatgc atgctcttca acctcaataa 11521 cgttatatcc tgattcacgt aatatttttg gggtaaattt agttcctgtt ccattaactg 11581 cgctaaaaat aatttttaaa tcttttttag cttcttgctc ttttttgtac gtctctgttt 11641 tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca 11701 ccgagtcggt gcttttttaa gcttggcgta actagatctt gagacaaatg gcagtattca 11761 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag 11821 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa 11881 attttcgggt ttattacagg gacagcagag atccactttg gcgccggctc gaggggg //